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Depressor Responses of Rabbits to Atropine (Atropine의 가토혈압하강작용(家兎血壓下降作用)에 관하여)

  • Kim, Jeong-Ja
    • The Korean Journal of Pharmacology
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    • v.11 no.1 s.17
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    • pp.19-26
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    • 1975
  • 1. Atropine has recently been known to possess a sympathetic ganglion blocking effect. If atropine blocks the sympathetic ganglia innervating the blood vessels, the drug should cause depressor responses. The author attempted to verify this assumption in urethane-anesthetized rabbits having atropinesterase. 2. Ten and $50{\mu}g/kg$ of atropine produced little variation of the blood pressure; $250{\mu}g/kg$ slight depressor responses; $1,250{\mu}g/kg$ distinct ones. Under hexamethonium-infusion, 10 and $50{\mu}g/kg$ produced observable depressor responses; 250 and $1,250{\mu}g/kg$ produced more pronounced ones. 3. In experiments examining influence of phenoxybenzamine and bretylium on the atropine responses, the lowered blood pressure by these agents was raised by simultaneous infusion of angiotensin with hexamethonium. The depressor responses to atropine (10, 50 and $250{\mu}g/kg$) were slight after the administration of phenoxybenzamine and bretylium. 4. Propranolol did not affect the depressor responses to atropine. 5. In spinalized rabbits the lowered blood pressure was raised by the angiotensin-infusion. In these animals receiving the simultaneous hexamethonium-infusion, atropine (10, 50 and $250{\mu}g/kg$) produced little depressor responses. 6. From these results it is inferred that atropine produced the depressor responses by blocking the sympathetic ganglia innervating the blood vessels.

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Optimum stocking density of juvenile abalone, Haliotis discus hannai in recirculating culture system (순환여과 사육시스템에서 참전복의 적정 사육밀도)

  • 손맹현;조기채;김경길;전임기
    • Journal of Aquaculture
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    • v.16 no.4
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    • pp.257-261
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    • 2003
  • The effects of different stocking densities on the growth of juvenile abalone, Haliotis discus hannai and water quality in the recirculating system with rotating biological contactor, were assessed. The trials were conducted using total weight 32 g juveniles abalone for 60 days at stocking densities of 5, 10, 15 kg/$m^2$. The animals were fed sufficient amounts of the pellet diet of 30.4% protein during the experimental period. Daily feeding rate (DFR) and survival rate (SR) of 5 kg/$m^2$ showed 0.76% and 85.5% those were not significantly differed 10 kg/$m^2$ showing 0.75% and 96.0% (P>0.05). DFR and SR of 15 kg/$m^2$ showing 0.38% and 31.2% were significantly lower than 5 and 10 kg/m2 (P<0.05). Daily growth rate (DGR) and feed efficiency (FE) showed the highest as 0.19% and 24.2% in 5 kg/$m^2$ the lowest as 0.05% and 14.3% in 15 kg/$m^2$ (P<0.05). According to, these results was an inverse relationship between growth and stocking density. The optimum density of juvenile abalone from this experiment is 5 to 10 kg/$m^2$ in the recirculating system.

A Toxicological Study of Young Fronds of Bracken Fern (Pteridium aquilinum var latiusculum) Collected in Kwang Ju Area (한국산 고사리의 독성조사에 관한 연구)

  • Sheo, Hwa-Joong;Lee, Myung-Yul
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.18 no.3
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    • pp.255-264
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    • 1989
  • The results of toxicity test using mice and rats for ethanol extract of Bracken Fern collected in Kwang Ju area were shown as follows ; Up to the dose of 10g per kg of mice administered intraperitoneally there was no lethal toxicity so that it was impossible to calculate the median lethal dose $(LD_{50})$. For the first 7 days experiment all rats administered frond extract grouping in 40mg, 400mg, and 1200mg per kg of rat as the daily oral doses did not show any characterized sign in the weight gain rate, anatomical findings, and biochemical studies. For 3 weeks following the first week the weight gain rates of all test group were reduced to $4.2{\sim}7%$ below the weight gain rate of control. In this period serum GPT, GOT, and Alkaline phosphatase value were increased significantly indicating the symptoms of Bracken Fern poisonings. The pathological findings of all test groups for 28 days showed acute and chronic intestinal lesion and liver damage with steatosis especially in 1200g/kg rat groups. In this experiment the Bracken poisonings appeared slowly in rats of 400mg/kg and 1200mg/kg for two weeks and in rats of 40mg/kg for 3 weeks, showing the symptoms of lowering of weight gain rate, subacute hepatitis, hepatic steatosis and enteritis in 28 days experiment.

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Allometric Equations for Crown Fuel Biomass of Pinus koraiensis Stands in Korea (잣나무림의 수관연료량 추정을 위한 상대생장식 개발)

  • Kim, Sungyong;Jang, Mina;Lee, Byungdoo;Lee, Youngjin
    • Journal of Korean Society of Forest Science
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    • v.104 no.1
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    • pp.104-110
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    • 2015
  • The objective of this study was to develop allometric equations for the estimation of crown fuel biomass of Pinus koraiensis in Korea. A total of twenty four representative sample trees were destructively sampled in Gapyeong, Hongcheon, and Jeongseon. Crown fuels were weighed separately for each fuel category by size class and by living and dead. The results of this study showed that the needles contributed the largest biomass (16.6 kg, 34.7%), followed by live branches with size ranging from 2~4 cm (9.0 kg, 18.9%), 1~2 cm (6.6 kg, 13.8%), <0.5 cm (5.1 kg, 10.6%), 0.5~1 cm (4.9 kg, 10.3%), and dead branches (3.2 kg, 6.8%), while the live branches with 4 cm (2.4 kg, 4.9%) as the lowest. The adjusted coefficient of determination values were the highest ($R^2_{adj}=0.6021{\sim}9742$) and standard error of estimate were the lowest (S.E.E.=0.2018~0.7271) in allometric equation $lnWt={\beta}_0+{\beta}_1lnD$. The available fuels that are consumed during crown fires (i.e., needles and twigs with diameter less than 1 cm) comprised 55.6% of the total crown fuel biomass.

Arsenic Speciation and Risk Assesment of Hijiki (Hizikia fusiforme) by HPLC-ICP-MS (HPLC-ICP-MS를 이용한 톳의 비소 화학종 분석 및 위해성 평가)

  • Ryu, Keun-Young;Shim, Sung-Lye;Hwang, In-Min;Jung, Min-Seok;Jun, Sam-Nyeo;Seo, Hye-Young;Park, Jong-Seok;Kim, Hee-Yeon;Om, Ae-Sun;Park, Kyung-Su;Kim, Kyong-Su
    • Korean Journal of Food Science and Technology
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    • v.41 no.1
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    • pp.1-6
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    • 2009
  • This study investigated arsenic speciation and risk assesment in 30 samples of hijiki purchased from local market in 10 Korean cities. The mean arsenic concentration of the hijiki samples was 45.65 mg/kg (dryness; moisture content of 91.1${\pm}$1.6%), and the major arsenic compound was arsenate [As(V)]. The concentrations of As(V) and As(III), as inorganic arsenic compounds, were detected to be 40.36 mg/kg and 0.37 mg/kg, respectively, and made up 88.6% (40.46 mg/kg) of the arsenic in the hijiki. Among the samples, the highest inorganic arsenic concentration was identified at 9.19 mg/kg (wet), and for an adult with a body weight of 60 kg was within an acceptable level as 0.7% (6.43 mg/60 kg/week) when compared with the provisional tolerable weekly intake (PTWI) (900 mg/60 kg/week), and would be considered safe with respect to health-hazardous effects.

The Formaion of N-nitrosamine in Soy Sauce, Soybean Paste and Beer under Simulated Gastric Digestion (간장, 된장 및 맥주의 인공소화시 N-nitrosamine의 생성)

  • Kim, Kyung-Ran;Lee, Soo-Jung;Shin, Jung-Hye;Seo, Jong-Kwon;Shon, Mi-Yae;Sung, Nak-Ju
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.3
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    • pp.378-383
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    • 2002
  • The aim of the study was to analyze N-nitrosamine (NA) and its precursors in serveral fermented foods which were treated with nitrite, thiocyanate and ascorbic acid under simulated gastric digestion. Every analyzed sample contained nitrate, with levels ranging from 0.3 to 1.3 mg/kg, but nitrite was present at very low levels of less than 0.3 mg/kg. And other precursors of amines such as dimethylamine and trimethylamine were detected less than 0.5 mg/kg in every samples. N-nitrosodimethylamine (NDMA) was detected in the levels of <0.5 ∼ 2.7 ug/kg in soy sauce,1.5∼3.1 ug/kg in soybean paste and <0.5∼1.8 ug/kg in beer, while NDMA levels increased by 1.1∼4.5 times in the fermented foods which were digested under simulated gastric conditions.

Effect of Wollastonite and Nitrogen Application on the Growth of Rice Plant (퇴화염토에서 수도생육에 대한 질소와 규회석의 시용효과)

  • 김규진;이주열
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.26 no.4
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    • pp.279-286
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    • 1981
  • This study was conducted to find out the effect of wollastonite application in degraded saline soil condition on the growth of japonica rice variety. The variety, Palgweng was tested with 4 levels of wollastonite (200, 400, 600 and 400/200 kg/l0a) and two nitrogen levels (12 and 16 kg/l0a). The effect of wollastonite was higher in 16 than 12 kg of nitrogen application. The wollastonite application reduced rice leaf blast and dead left at lower nodal position, and increased percentage of effective tillers, percentage of ripened grains and 1, 000 grain weight. The SiO$_2$ content of upper leaf was higher than lower leaf in 200 kg of wollastonite application, but there was no difference under high wollastonite application (400 kg/l0a). Highly significant positive correlationships were obtained between SiO_2 content of plant at heading and percentage of ripened grains, (r=0.613) and SiO_2 content of flag leaf and percentage of ripened grains (r=0.407). Wollastonite application did not affect the development and degeneration of primary and secondary rachis branches of panicles, but it increased the number of spikelets. The highest grain yield was obtained with 16 kg of nitrogen and 600 kg of wollastonite.

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Air Drying Technology for Dewatered Cake from Wastewater and Waterworks Sludge (상.하수 슬러지 탈수케이크의 공기건조에 관한 연구)

  • Lee, Jung-Eun;Cho, Eun-Man;Kim, Bong-Hwan
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.11
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    • pp.1154-1161
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    • 2006
  • Air drying equipment was built as a device for reduction of dewatered cake from wastewater and waterworks sludge and to reproduce it by reusable matter. Dewatered cake was supplied into the air drying equipment which operated by air velocity of 80 m/sec, air rate 30 $m^3/min$ and air temperature of $40^{\circ}C$, and dried to produce the dried powder. The air drying equipment was composed of the air ejector which made high-speed fluid field, and cyclone which made circling fluid field. Dewatered cake was crushed at the high-speed zone as first step, and formed into dried powder of sphere shape by the collision between particles at the circling fluid zone.. Wastewater sludge with water content of 82.5 wt% was supplied 1.0 kg/min into air drying equipment and produced the dried powder which had the water content of 62.3 wt% and mass median diameter of 2.4 mm after process. At that time, it was analyzed that water removal rate was 0.1 $H_2O{\Delta}kg/min{\cdot}DS$ kg and air consumption was 170 $m^3/DS$ kg. Under same experimental conditions, when waterworks sludge was dried, water content of dried powder decrease to 47.5 wt% and mass median diameter decrease 2.1 mm and water removal rate increase 0.13 $H_2O{\Delta}kg/min{\cdot}DS$ kg. Air consumption increase 180 $m^3/DS$ kg with comparison to the results of wastewater sludge. Therefore, this technology was evaluated that drying the dewatered cake of waterworks sludge was more efficient than wastewater sludge, and also economical sludge handling technology due to drying the cake by only air.

Determination of Nitrogen Balance of Agricultural Land among OECD Nutrient Balance Indexes (OCED 농업양분지표중 질소 균형지표 설정)

  • Lee, Chun-Soo;Kim, Pil-Joo;Park, Yang-Ho;Kwak, Han-Kang
    • Korean Journal of Soil Science and Fertilizer
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    • v.33 no.5
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    • pp.347-355
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    • 2000
  • To determine nitrogen balance (Input-Output) of Korea that was asked by Joint Working Party of the Committee for Agriculture and the Environment Policy Committee, OECD, nitrogen input and output were separately investigated as followings: nitrogen input included the amounts of chemical fertilizer consumption, cattle manure production, and biological nitrogen fixation in the national scale, and nitrogen output summed amounts withdrawn by crop and pasture harvesting, and crop residue removal, and lost by denitrification. In 1997, nitrogen balance of Korea was $158kg\;ha^{-1}$ and $211kg\;ha^{-1}$ on including and excluding denitrification loss, respectively. N balance excluded N loss by denitrification and N withdrawal by crop residues on nitrogen output was $250kg\;ha^{-1}$, which OECD asked to except two items from N balance determination because participants were not enough their data. Nitrogen balance was increased to 1.7~2.3 times in 1997 compared with 70 and $162kg\;ha^{-1}$ in 1985, which calculated on the condition of including denitrification and excluding denitrification and removal of crop residues in nitrogen output, respectively. This increase was caused mainly by increasing livestock manure production and chemical fertilizer consumption together with agricultural land area decrease. Nitrogen input was composed with 59% of chemical fertilizer. 42% of cattle manure and 5% of others in 1997, and output was with 73% of crop production, 23% of crop residue withdrawal and 4% of pasture production. Average nitrogen balance excluded N loss by denitrification and N withdrawals by crop residues in 1995~1997 calculated by OECD was $253kg\;ha^{-1}$, which was the second highest rank in OECD participants following $262kg\;ha^{-1}$ of Netherlands. Japanese N balance that has similar farming system with us was $135kg\;ha^{-1}$.

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In vivo Antitumor Activity and Acute, Subacute Toxicity of Keumsa (Phellinus linteus) Extracts (금사목질진흙버섯 자실체 추출물의 in vivo 항암활성 및 급성, 아 급성 독성 시험)

  • Kim, Jong-Myeung;Park, Jun-Duck;Park, Dong-Chan;Kim, Byung-Oh
    • Journal of Life Science
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    • v.23 no.11
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    • pp.1388-1396
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    • 2013
  • This study used an S-180 cell-injected mouse model to evaluate the antitumor effects of the acute and subacute toxicity of Keumsa (Phellinus linteus) extract intravenously administrated in ICR mice. When administered intravenously (31.3-250 mg/kg body weight), Keumsa (Phellinus linteus) extract significantly inhibited the growth of the solid tumor cell. The antitumor activity of Keumsa (Phellinus linteus) extract increased in a dose-dependent manner. The highest dose (250 mg/kg body weight) was highly effective, reducing tumor formation by 42.7% compared with the control group. In the acute toxicity test, $LD_{50}$ of the Keumsa (Phellinus linteus) extract showed 632.84 mg/kg (♂) and 814.48 mg/kg (♀) after intravenous administration. In addition, liver and spleen weight were increased in a dose-dependent manner. In the subacute toxicity test, the mice were intravenously administered over the course of 28 days. The $LD_{50}$ of the Keumsa (Phellinus linteus) extract showed 355.41 mg/kg (♂) and 383.53 mg/kg (♀) after intravenous administration. The liver and spleen weight also increased in a dose-dependent manner. In the case of the group that received more than 125 mg/kg of intravenous administration, exercise capacity, such as jumping ability and agility, were significantly increased. These results suggest that Keumsa (Phellinus linteus) extract can be regarded as a potent enhancer of the innate immune response, and it can be considered as a new natural product with low toxicity that may be used as a candidate for antitumor action.