• Advances in Traditional Medicine
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• 제7권3호
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• pp.261-273
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• 2007

We investigated the antihypertensive effect of Pet ether extract (PE) of ginger rhizome; its toluene fraction (TF) and Korean ginseng extract (KGE) in deoxycorticosterone acetate (DOCA) - salt induced and fructose induced hypertensive rats. In DOCA model, DOCA (25 mg/kg, once a week; s.c) was administered in uninephrectomised animals for 4 w. PE (50 mg/kg/day; p.o), TF (10 mg/kg/day; p.o) and KGE (30 mg/kg/day; p.o) were evaluated for their antihypertensive effect. In the fructose model, drinking water was replaced with fructose (10%) for five weeks to induce hypertension. PE (50 mg/kg/day; p.o) and KGE (30 mg/kg/day; p.o) were assessed for its antihypertensive effect in fructose model. After completion of the treatment schedule, vascular reactivity to various agonists like 5-HT, noradrenaline, adrenaline, phenylbiguanide and acetylcholine were recorded in rats of both the models. A cumulative dose response curve (CDRC) of 5-HT was carried out in isolated rat fundus strip of the fructose induced hypertensive rats. Chronic administration of PE (50 mg/kg/day; p.o), TF (10 mg/kg/day; p.o), and KGE (30 mg/kg/day; p.o) significantly reduced the blood pressure in DOCA salt whereas PE (50 mg/kg/day; p.o) and KGE (30 mg/kg/day; p.o) reduced the blood pressure in fructose induced hypertensive rats. Treatment with PE (50 mg/kg/day; p.o) and KGE (30 mg/kg/day; p.o) in fructose model for five weeks shifted the CDRC towards the right on rat fundus. The mechanism of action may partly involve the serotonergic antagonistic property.

• 한국식품영양학회지
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• 제18권3호
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• pp.254-264
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• 2005

This study was conducted to estimate the contents of heavy metals 'Hg, Cd, Pb, As, Zn, Cu, Cr, Mn' in some vegetables which were produced in Korea. The levels of heavy metals were determined using a mercury analyzer, an ICP(inductively coupled plasma spectrometer) and an AAS(atomic absorption spectrophotometer) after wet digestion. The values of heavy metals "mean(mini.~maxi.)" ppm(mg/kg) in some vegetables(raw of perilla leaf, chard, small water dropwort, water dropwort, kale, bud of aralia, pumpkin(round type) and pumpkin(long type) were as follows : Hg : 0.0021(0.0006~0.0054)mg/kg, Cd : 0.0035(ND*~0.0377)mg/kg, Pb : 0.0191(0.0023~0.0928)mg/kg, As : 0.0757(ND~0.5294)mg/kg, Zn : 2.6299(0.4478~6.8567)mg/kg, Cu : 1.0261(0.2046~8.9417)mg/kg, Cr : 0.1535 (0.0240~0.4982)mg/kg, Mn : 3.2476(0.3283~9.8280)mg/kg. This results showed that Mn was generally simillar to previous reports and Cd, Hg, Pb were lower than other reseaches, but As was little higher or Zn, Cu, Cr were higher than the levels of those reported contents in some vegetables on domestic supermarkets in Korea, Although tolerable limit of Hg and Cd is not in a regulation of WHO/FAO, these mean levels(Pb, As, Zn, Cu) are lower than recommended levels of WHO/FAO, Pb "0.1~2.0" mg/kg, As "1.0" mg/kg, Zn "5.0" mg/kg and Cu "0.1~50"mg/kg from vegetables in 'the tolerable contents of food' by the FAO/WHO, therefor some vegetables has set to evaluate their safeties.

• 한국초지조사료학회지
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• 제15권3호
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• pp.164-168
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• 1995

A field experiment was carried out to determine the effects of nitrogen (N) fertilization levels after first harvest (0, 30, 60, 90 and 120 kglha) during spring season on the gms growth, dry matter (DM) yield, nutritive value, and efficiency of DM production per N kg applied in orchardgrass meadow. Additional N was applicated at early spring (70 kg), after second harvest (50 kg), and after fourth harvest (30 kg/ha) in all treatments, 1990. Growth and DM yield of orchardgrass at second harvest were significantly increased with increasing level of N after first harvest (P<0.05), but no yield differences were observed between application of N 60, N 90, and N 120 kg. The efficiency of DM increment per N kg applied was rapidly decreased with increasing level of N; that was 14.8, 12.0, 10.7 and 4.7 DM kg/N kgha in the plot of N 30, N 60, N 90 and N 120 kg, respectively. The content and yield of crude protein of orchardgrass at second harvest were significantly increased with increasing of N (P<0.05), ranged h r n 11.0% (N 0 kg) to 16.8% (N 120 kg), and from 195.0 kg (N 0 kg) to 508.2 kglha (N 120 kg), respectively. However, the content of neutral detergent fiber was not affected by N level after first harvest. From the above results, it is suggested that application of N fertilizer after first harvest was very effective for regrowth and nutritive value of orchardgrass during spring season, and the proper amount of N after first harvest was 60-90 kglha in this experiment.

• 한국자원식물학회지
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• 제15권1호
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• pp.67-71
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• 2002

삼백초의 3요소 시비 적량을 구명하고자 3요소 시비수준을 무비구 등 3요소별 4수준으로 11조합하여 시험을 수행한 결과를 요약하면 다음과 같다. 1. 토양의 이화학적 변화는 pH, 유기물, 마그네슘, CEC 그리고 EC는 시험 전에 비해 시험 후에 높은 경향을 보였으나, 인산, 칼리 그리고 칼슘은 상반된 경향이었다. 2. 건엽중은 무비구 281kg/10a에 비하여 3요소 시비구에서 26~108kg가 많았으며, 건근중은 무비구 610kg/10a에 비하여 3요소 시비구에서 19~157kg가 많았다. 3. 2년생 삼백초의 3요소 시비적량은 질소 7.1, 인산 2.5, 칼리 4.2kg/10a이었다.

• 한국축산식품학회지
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• 제35권5호
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• pp.646-652
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• 2015

The beef forequarter muscle comprises approximately 52% of carcass weight. The objective of this study was to evaluate the physiochemical characteristics and meat color from forequarter muscle of Holstein steers. Fifteen forequarter muscles were trimmed of external connective tissue and fat. An experimental group of eight Holstein steers was assessed using meat color, water-holding capacity, drip loss, and Warner-Bratzler shear force value at the same quality grade. The M. omotransversarius (0.45 kg) had the highest (p<0.05) lightness (L*) value, whereas the M. teres major (0.4 kg) and M. triceps brachii (caput laterale) (0.52 kg) had the lowest (p<0.05) values. The M. semispanitus capitus (1.48 kg), which is a neck muscle, had the highest values for both redness (a*) and yellowness (b*), whereas the lowest (p<0.05) values were for the M. teres major. The M. omotransversarius, M. latissimus dorsi (1.68 kg), and M. rhomboideus (1.2 kg) were ranked high (p<0.05) in water-holding capacity. The drip loss value was the highest for the M. longissimus dorsi thoracis (p<0.05; 1.86 kg), while the M. infraspinatus (2.28 kg), M. supraspinatus (1.38 kg), M. brachiocephalicus (1.01 kg), and M. pectoralis superficialis (1.18 kg) had the lowest (p<0.05). The Warner-Bratzler shear force value indicated that the M. pectoralis profundus (3.39 kg), M. omotransversarius, and M. brachiocephalicus were the toughest (p<0.05), whereas the M. subscapularis (0.86 kg), M. longissimus dorsi thoracis, M. teres major, and M. infraspinatus were the most tender cuts (p<0.05). Here, muscle type explained most of the variability in the forequarter physiochemical characteristics. Thus, our findings suggest that these muscle profile data will allow for more informed decisions when selecting individual muscles to produce value-added products from Holstein steers.

• The Korean Journal of Ecology
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• 제15권1호
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• pp.35-46
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• 1992

To elucidate nutrient cyclings such as nitrogen, phosphorus and potassium in mongolian oak(quercus mongolica) forest, nutrient elements of precipitation, throughfall, outflow, soil, various plant organ and litter were determined at mt.nambyeongsan, pyeongchang-gun, gangwon province in central part of korean peninsula. Annual precipitation input, throughfall and outflow of nutrientswere 10.3, 8.6 and 4.2 kg/ha for the N, 0.11, 0.24 and 0.02 kg/ha for the Pand 1.3, 10.9 and 1.2 kg/ha for the K, respectively. Inseasonal changes of nutrient concentrations, N, P and Kconcentrations which were rich in young leaves decreased steadily until autumn and decreased abruptly during autumnal yellowing. The standing N, P and K concent were 565, 37 and 257 kg/ha for standing phytomass of overstory, 33, 3 and 18 kg/ha for understory, 132, 3.6 and 14 kg/ha for litter on ground including deadwood and 20, 752, 14 and 420 kg/ha for the soil, respectively. The amounts of annual uptake, reture and retain were 174.2, 57.2, 117.2 kg/ha for the N, 9.9, 3.5, 6.4 kg/ha for the P and 73.2, 30.3, 42.9 kg/ha for the K, respectively. Reabsorption efficiency, ratio of the nutrient amount reabsorbed into woody organs to that in the mature leaves before shedding, was 71%(or 99.8 kg/ha in the amount), 69%(or 5.1 kg/ha) and 57%(or 33.1% kg/ha) and recycling coeffciently made with which the large amount of nutrients is absorbed through roots during growing season(UPTAKE) and reasorbed from the leaves before shedding(RETAIN) but the small amount of nutrients is returned through litterfall(RETURN).

• 한국생물환경조절학회:학술대회논문집
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• 한국생물환경조절학회 2001년도 봄 학술발표논문집
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• pp.69-70
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• 2001

포도주 부산물이 퇴비와 혼합처리 시용에 따른 고추생육에 미치는 영향을 구명하기 위해 평창군 비가림재배 시설하우스 농가에서 수행하였다. 초기 생육기인 정식 후 15에는 고추의 초장은 유의차가 인정되지 않았으나 정식 후 기간이 경과함에 따라 높은 증가추세를 나타내었으며, 경경의 경우 정식 후 45일, 75일에는 유의차(p>0.05)가 인정되었다. 대조구(돈분발효퇴비 2,000kg/10a)가 0.53cm에서 1.67cm로 1.14cm 증가하였는데, 처리구 T4(포도주부산물 400kg＋돈분발효퇴비 1,000kg/10a)는 0.5cm에서 1.74cm로 1.24cm 증가하여 대조구에 비하여 8.7% 높은 증가율을 보였다. 각 처리구의 엽록소 함량은 45일에 증가하다가 75일에 다시 감소하는 현상을 나타내었으며, 처리 15일에서 T1(포도주부산물 200kg＋돈분발효퇴비 2,000kg/10a)과 대조구 사이에 유의차가 인정되었으며, T1(포도주부산물 200kg＋돈분발효퇴비 2,000kg/10a)이 대조구(돈분발효퇴비 2,000kg/10a)보다 2.6이 높은 56.1로서 가장 높았으며, T4(포도주부산물 400kg＋돈분발효퇴비 1,000kg/10a)에서는 53.4로 대조구와 같은 수준을 나타내었다. 엽면적은 정식 후 45일에서 T4(포도주부산물 400kg＋돈분발효퇴비 1,000kg/10a)가 60.0$\textrm{cm}^2$로 가장 높게 나타났다. 홍고추의 주당 수량은 돈분발효퇴비와 포도주부산물을 같이 시비한 처리구가 대조구(돈분발효퇴비 2,000kg/10a)에 비하여 감소현상을 보였는데 이러한 현상은 생육후반기에 암모니아 가스에 의해 작물의 피해가 발생하여 수량이 감소된 것으로 사료된다.

• Journal of Ginseng Research
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• 제46권2호
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• pp.283-289
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• 2022

Background: Sarcopenia, defined as loss of muscle mass and strength with age, becomes a public health concern as the elderly population increases. This study aimed to determine whether the mixture of soluble whey protein hydrolysate (WPH) and Panax ginseng berry extract (GBE) has a synergetic effect on sarcopenia and, if so, to identify the relevant mechanisms and optimal mixing ratio. Methods: In the first experiment, C57BL/6 mice were hindlimb immobilized for one-week and then administered WPH 800 mg/kg, GBE 100 mg/kg, WPH 800 mg/kg+ GBE 100 mg/kg mixture, and Fructus Schisandrae extract (SFE) 200 mg/kg for two weeks. In the second experiment, experimental design was same, but mice were administered three different doses of WPH and GBE mixture (WPH 800 mg/kg+ GBE 100 mg/kg, WPH 800 mg/kg+ GBE 90 mg/kg, WPH 1000 mg/kg+ GBE 75 mg/kg). Results: In the first experiment, we confirmed the synergetic effect of WPH and GBE on muscle mass and identified that GBE was more effective on the protein synthesis side, and WPH tended to be slightly more effective for protein degradation. In the second experiment, among three different ratios, the WPH 800 mg/kg+ GBE 100 mg/kg was most effective for muscle mass and strength. The mixtures activated muscle protein synthesis via PI3K/Akt/mTORc1 pathway and inhibited muscle protein degradation via suppressing ubiquitin-proteasome system (UPS) and autophagy-lysosome system (ALS), and these effects were more GBE dose-dependent than WPH. Conclusion: The WPH and GBE mixture having a synergetic effect is a potential agent to prevent sarcopenia.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.615-620
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• 2003

Gyrodinium impudicum strain KG03의 세포외 다당류인 p-KG03은 황함유 다당류로 항바이러스 검색 결과, 조사대상 바이러스 중 특히 뇌심근 경색바이러스 (encephalomyocarditis virus; EMCV)에 항바이러스 활성을 가지는 것으로 조사되었다.

• Biomolecules & Therapeutics
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• 제5권1호
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• pp.12-22
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• 1997

Intravenous and oral acute toxicity tests in ICR mice and SD rats and percutaneous acute toxicity tests in SD rats and NZW rabbits were conducted to evaluate the toxicity of DA-5018 and DA-5018 cream, respectively Clinical signs observed in mice and rats after the administration of DA-5018 were similar regardless of administration route. The observed clinical signs were jumping, wild running, lacrimation, ataxia, reddening of extremities and ears, ventral or lateral recumbency, respiratory distress, cyanosis, convulsion and death. Pulmonary enlargement and hemorrhage were observed in the animals died immediately after the dosing of DA-5018. At terminal necropsy, pulmonary enlargement and hemorrhage, corneal opacity and focal scabbing and depilation around nose were seen. LD$_{50}$ Values of DA-5018 are 11.5 mg/kg (mice, male), 12.6 mg/kg (mice, female), 88.3 mg/kg (rat, male) and 73.2 mg/kg (rat, female) in oral toxicity tests and 11.0 mg/kg (mice, male), 18.7 mg/kg (mice, female), 0.12 mg/kg (rat, male) and 0.32 mg/kg (rat, female) in i.v. toxicity tests. In the percutaneous acute toxicity tests of DA-5018 cream, no deaths occured in all the tested groups during 14-day observation period. There were also no abnormalities in the general conditions, body weight changes and on necropsy findings in all groups. LD$_{50}$ values of 0.1 ~0.9% DA-5018 creams in male and female rats and rabbits are >2000 mg/kg./kg.