Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Synergetic effect of soluble whey protein hydrolysate and Panax ginseng berry extract on muscle atrophy in hindlimb-immobilized C57BL/6 mice

  • Han, Min Ji (Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University) ;
  • Shin, Ji Eun (Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University) ;
  • Park, Seok Jun (Health & Nutrition R&D Group, Maeil Dairies Co., Ltd.) ;
  • Choung, Se-Young (Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University)
  • Received : 2021.05.12
  • Accepted : 2021.06.24
  • Published : 2022.03.01
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Abstract

Background: Sarcopenia, defined as loss of muscle mass and strength with age, becomes a public health concern as the elderly population increases. This study aimed to determine whether the mixture of soluble whey protein hydrolysate (WPH) and Panax ginseng berry extract (GBE) has a synergetic effect on sarcopenia and, if so, to identify the relevant mechanisms and optimal mixing ratio. Methods: In the first experiment, C57BL/6 mice were hindlimb immobilized for one-week and then administered WPH 800 mg/kg, GBE 100 mg/kg, WPH 800 mg/kg+ GBE 100 mg/kg mixture, and Fructus Schisandrae extract (SFE) 200 mg/kg for two weeks. In the second experiment, experimental design was same, but mice were administered three different doses of WPH and GBE mixture (WPH 800 mg/kg+ GBE 100 mg/kg, WPH 800 mg/kg+ GBE 90 mg/kg, WPH 1000 mg/kg+ GBE 75 mg/kg). Results: In the first experiment, we confirmed the synergetic effect of WPH and GBE on muscle mass and identified that GBE was more effective on the protein synthesis side, and WPH tended to be slightly more effective for protein degradation. In the second experiment, among three different ratios, the WPH 800 mg/kg+ GBE 100 mg/kg was most effective for muscle mass and strength. The mixtures activated muscle protein synthesis via PI3K/Akt/mTORc1 pathway and inhibited muscle protein degradation via suppressing ubiquitin-proteasome system (UPS) and autophagy-lysosome system (ALS), and these effects were more GBE dose-dependent than WPH. Conclusion: The WPH and GBE mixture having a synergetic effect is a potential agent to prevent sarcopenia.

Keywords

Acknowledgement

This research was supported by the Bio and Medical Technology Development Program of the National Research Foundation of Korea funded by the Ministry of Science & ICT (the Bio & Medical Technology Development Program, 2018M3A9F3081447).

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