• Korean Journal of Food Science and Technology
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• v.28 no.5
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• pp.953-958
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• 1996

A simple and rapid ELISA (enzyme-linked immunosorbent assay) system for fumonisins, a group of potentent carcinogen, was developed. To produce anti-fumonisin B1 (FB1) antibodies, FB1 conjugated to keyhole lympet hemocyanin (KLH) and Freund's adjuvant were immunized into rabbits subcutaneously 3 times. From one of the antisera showing high titer and good competition with the toxin in ELISA, polyclonal antibodies were purified. The cross-reactivities of the antibodies against fumonisin $B_1,\;B_2\;and\;B_3$ were 100%, 69%, and 166%, respectively. When competitive direct ELISA established by use of the antibody was applied to the spike test of $FB_1$ onto uncontaminated corns, the assay recovery was unstable unless 75% methanol extracts of corn were diluted to 1/100 with buffer. In that condition the mean ELISA recovery of FB1 from corns spiked $1-30\;{\mu}g/g$ was 67% and stable (coefficient of variation (CV) of each recovery percentage, 3.4%). The results suggest that the ELISA system established in this study needs no cleanup procedure and therefore would be powerful to screen a large number of corn samples contaminated with fumonisins.

• Journal of Acupuncture Research
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• v.22 no.3
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• pp.23-35
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• 2005

Objective : The aim of the study is to investigate the effect of electro-acupuncture (EA) on ST36 to modulate immune reaction in BALB/c mice immunized intraperitoneally with 2,4-dinitrophenylated keyhole limpet protein(DNP-KLH). Methods : Experimental mice were divided into four groups : 1) Normal group was not performed by any operation. 2) IM(Immunized) group was immunized intraperitoneally with DNP-KLH and aluminum hydroxide without electro-acupunture stimulation. 3) IM-EA(immunized-elctro- acupuncture) group was performed by successive electro-acupuncture on the ST36 acupoint after immunization. 4) IM-NA(immunized-naloxone) group was performed by immunization and electro-acupuncture with same method, but naloxone was injected intraperitoneally 30 minutes before eletro-acupuncture to inhibit the opiate receptor in spleen. Serum total immunoglobulin I(IgE) and antigen-specific IgE was measured in each group. The expression of interferon-${\gamma}$ and interleukin-4 mRNA in spleen was researched by real-time RT-PCR Results : Serum total-IgE and antigen-specific IgE were significantly decreased only in IM-EA group. The expression of interleukin-4 in spleen cell was significantly reduced not only in IM-EA group, but also in IM-EA group. Conclusions : Above results indicate that the mechanism of immunomodulatory effect of electro-acupuncture is related to opioid system especially in B-cell immune reaction. Further research on the T-cell immunity is necessary to explain the mechanism of immunomodulatory effect of electro-acupuncture.

• The Korean Journal of Pesticide Science
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• v.5 no.2
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• pp.1-12
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• 2001

A competitive indirect enzyme-linked immunosorbent assay (ci ELISA) for the organophosphorus insecticide acephate, O,S-dimethyl acetylphosphoramidothioate, was developed using a polyclonal antibody. Three different haptens mimicking the analyze and containing hexanoic acid moiety as a linker were synthesized, and then conjugated with the carrier proteins bovine serum albumin and keyhole limpet hemocyanin by the N-hydroxysuccinimide active ester method. Polyclonal antibodies raised against hapten-KLH conjugates in rabbits and the hapten-BSA conjugates as coating antigens were screened and selected for the assay in the homologous and/or heterologous ELISA system. The effects of various assay conditions, including blocking reagents, detergent content, organic solvents, pH, and preincubation of tile mixture of the polyclonal antibody and the analyze on the sensitivity were evaluated. The $IC_{50}$ value of acephate of 110 ng/mL was obtained in an optimized heterologous system using hapten-3-BSA as a coating antigen and a polyclonal antibody 8377, showing the detection range of 10-1000 ng/mL and the lowest detection limit of 4 ng/mL. The cross-reactivities of the structurally related insecticides, including methamidophos were less than 0.02%. These results indicate that the ELISA could be a convenient and alternative tool for monitoring acephate residues in agricultural products and environmental samples.

• Journal of Yeungnam Medical Science
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• v.4 no.2
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• pp.51-58
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• 1987

The goal of reduction mammaplasty is a breast with natural contour and volume, aesthetically situated scars, and a well-placed, sensate nipple and areola. The most successful techniques achieve this through the excision of tissue from the lower part of the breast based on some variation. However, the Mckissock's vertical bipedicle technique is the popular method for reduction mammaplasty. As an alternative modified Mckissock's method, we have found the use of only an inferior dermal flap with a keyhole pattern to be a simple and safe method for obtaining satisfactory aesthetic results. We have used inferior dermal flap for 4 patients recently and obtained the advantages as below compare to the Mckissock's method. 1. Rich blood supply to the broad based inferior flap. 2. More easy transposition of the nipple and areola. 3. More good operation field for resection of breast tissue. 4. Short operation time. 5. Can applied to the gigantomastia.

• Journal of Cadastre & Land InformatiX
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• v.44 no.1
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• pp.165-177
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• 2014

The KML verification tool supports people who want to produce the highest quality KML file. In other words, it validate that a given KML document is well-formed with respect to XML standard meaning, and conform not only to the KML schema and the specification. Then it's only to notify error code line. People who want to use the KML file written by others would like to know both whether the validity of that file and general summary of feature's location, shape, and number. In this study, we recommended the user-friendly KML validator using OpenLayers and reporting geometries and images of the KML file.

• Journal of Welding and Joining
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• v.33 no.6
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• pp.60-66
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• 2015

Molten zone shape of pulse laser welding is affected by welding conditions such as beam power, beam speed, irradiation time, pulse frequency, etc. and is divided into conduction type and keyhole type. It is necessary to design heat source model for irradiation of laser beam in the pulse laser welding. Shape variables and the maximum energy density value of the heat source model are different depending on the molten zone shape. In this paper, pulse laser welding simulation for joining of cylindrical part and circular cover was carried out. The heat source model for pulse laser beam with circular path was applied to the heat input boundary condition, radiative and conductive heat transfer were considered for the thermal boundary condition. For each phase, thermal and mechanical properties according to temperature were also applied to analysis. Analytical results were in good agreement with the molten zone size of specimen under the same welding conditions. So, the reliability of the welding simulation was verified. Finally, the improvements for reducing residual deformation after cover welding could be reviewed analytically.

• Journal of Welding and Joining
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• v.28 no.6
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• pp.51-57
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• 2010

Fiber laser is a heat source which is introduced recently, and so has a little researched data compare with conventional laser processing. Moreover basic data for welding monitoring are also insufficient. Therefore, in this study, the change of signal with measuring position and angle of plasma emission signals were analysed as a basic experiment for real time monitoring in fiber laser welding. As a result, the signals measured from the side, front and rear had the biggest intensity at $60^{\circ}$, and frequency peak to reflect the behavior of keyhole and swing of plasma by shield gas was detected at $45{\sim}60^{\circ}$. However, both intensity of signal and the result of FFT for monitoring were satisfied at the angle of $45^{\circ}$ from the side.

• Journal of Welding and Joining
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• v.34 no.5
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• pp.26-32
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• 2016

In this study, Hot-wire laser welding (HWLW) without keyhole which deposits filler material by feeding hot wire into the process zone has been performed to increase process performance. From the analysis of High Speed Imaging (HSI), for higher voltage, the process is prone to arc formation and drop transfer, which is disagreeable transfer mode. It is necessary that arc formation and drop (globular) transfer should be avoided by lower voltage. Therefore, continuous wire melting and transfer mode is preferred when adopting this process. The HWLW technique has high potential in terms of performance, precision, robustness and controllability for thick section of narrow gap.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.25 no.4
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• pp.654-661
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• 2001

In this study, fatigue growth behavior of the transverse crack, which was the most dangerous damage among the various types of rail defects, was investigated using the notched keyhole specimen under constant amplitude loadings. Fatigue limit of smooth specimen in rail steel at R=0 was 110MPa, and the fatigue crack initiation life in the region of the low stress amplitude (ie. long life) occupied the major portion of the total fatigue life. The fatigue strength under variable amplitude loading was converted to the equivalent fatigue strength based upon. Miners rule, which was estimated approximately 9% lower than that under constant amplitude loading. Also, in the low ΔK(sub)rms region ($\leq$21MPa√m), fatigue crack growth rate (da/dN) under constant amplitude loading was higher than that under variable amplitude loading, whereas the tendency was reversed in the high ΔK(sub)rms region. It is believed that this behavior is due to the transition of fracture appearance.

• Journal of the Optical Society of Korea
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• v.14 no.4
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• pp.431-437
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• 2010

A stack of gradient-index (GRIN) rod lenses cannot be used for coherent anti-Stokes Raman scattering (CARS) microendoscopy for insertion to internal organs through a surgical keyhole with minimal invasiveness. That's because GRIN lens has large amount of inherent chromatic aberrations in spite of absolutely requiring a common focus for pump and Stokes beam with each frequency of ${\omega}_p$ and ${\omega}_S$. For this endoscopic purpose, we need to develop a long slender probe-type objective, namely probe-type microscope objective (PMO). In this paper, we introduce the structure, the working principle, and the design techniques of PMO which is composed of a probe-type lens module (PLM) and an adaptor lens module (ALM). PLM is first designed for a long slender type and ALM is successively designed by using several design parameters from PLM for eliminating optical discords between scanning unit and PLM. A combined module is optimized again to eliminate some coupling disparities between PLM and ALM for the best PMO. As a result, we can obtain a long slender PMO with perfectly diffraction-limited performance for pump beam of 817 nm and Stokes beam of 1064 nm.