• Asian-Australasian Journal of Animal Sciences
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• v.29 no.10
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• pp.1500-1507
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• 2016

Myostatin (MSTN) can negatively regulate the growth and development of skeletal muscle, and natural mutations can cause "double-muscling" trait in animals. In order to block the inhibiting effect of MSTN on muscle growth, we transferred zinc-finger nucleases (ZFN) which targeted sheep MSTN gene into cultured fibroblasts. Gene targeted colonies were isolated from transfected fibroblasts by serial dilution culture and screened by sequencing. Two colonies were identified with mono-allele mutation and one colony with bi-allelic deletion. Further, we introduced the MSTN-ZFN mRNA into sheep embryos by microinjection. Thirteen of thirty-seven parthenogenetic embryos were targeted by ZFN, with the efficiency of 35%. Our work established the technical foundation for generation of MSTN gene editing sheep by somatic cloning and microinjection ZFN into embryos.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.43 no.1
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• pp.50-60
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• 2020

The omni-channel customer system is the communication system between enterprise and customer via multiple channels such as mail, email, SMS, and mobile. The omni-channel customer system complements each other channel through the integration of each channel. The purpose of this research is to derive key factors and calculate the weights that a financial enterprise considers when adopting the omni-channel customer system. For this research, we analyzed the request for proposal documents used for the omni-channel customer system implementation projects in the financial enterprise. Also, we derived, classified, and stratified the key factors to be considered for the introduction of the omni-channel customer system in the financial enterprise. As a result of analyzing the key factors, customer experience, operations, and security were identified as the components of the top category in introducing the omni-channel customer system in the financial sector. Furthermore, the weight for each key factor was calculated by using ANP. As a result of ANP, operations, customer experience, and security were important in order. Also, the degree of easiness for connecting with other systems and the various abilities for representing the contents of the omni-channels were derived as the important key factors.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.2 no.1
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• pp.43-50
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• 2009

This paper caters the need of acquiring the principal objects, characters, and scenes from a video in order to entertain the image based query. The movie frames are divided into frames with 2D representative images called "key frames". Various regions in a key frame are marked as key objects according to their textures and shapes. These key objects serve as a catalogue of regions to be searched and matched from rest of the movie, using viewpoint invariant regions calculation, providing the location, size, and orientation of all the objects occurring in the movie in the form of a set of structures collaborating as video profile. The profile provides information about occurrences of every single key object from every frame of the movie it exists in. This information can further ease streaming of objects over various network-based viewing qualities. Hence, the method provides an effective reduced profiling approach of automatic logging and viewing information through query by example (QBE) procedure, and deals with video streaming issues at the same time.

• Proceedings of the Korea Society of Information Technology Applications Conference
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• 2005.11a
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• pp.277-280
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• 2005

With the proliferation of mobile terminals, use of the Internet in mobile environments is becoming more common. To support mobility in these terminals, Mobile IPv4 is proposed and represents the standard in IPv4 environments. Authentication should be mandatory, because mobile terminals can utilize Internet services in any foreign domain. Mobile IPv4 provides symmetric key based authentication using the default HMAC-MD5. However, symmetric key based authentication creates a key distribution problem. To solve this problem, public key based authentication mechanisms have been proposed. In this paper, the performance of each of these mechanisms is evaluated. The results present that, among these mechanisms, partial certificate based authentication has superior performance, and certificate based authentication has the worst performance. Although current public key based authentication mechanisms have lower performance than symmetric key based authentication, this paper presents the possibility that public key based authentication mechanisms may be used for future mobile terminal authentication.

• Journal of the Korea Institute of Military Science and Technology
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• v.12 no.4
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• pp.476-482
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• 2009

As a result from Hasse's theorem it is not always possible to share a common key between any two ECC public keys. Even though ECC algorithm is more efficient than any other Encryption's with respect to the encryption strength per bit, ECC ElGamal algorithm can not be used to distribute a common key to ECC PKI owners. Approaching mathematical ways in a practical situation, we suggest possible conditions to share a common key with ECC PKI's. Using computer experiments, we also show that these suggestions are right. In the conditions, we can distribute a common key to proper peoples with ECC ElGamal algorithm.

• International Journal of Advanced Culture Technology
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• v.10 no.3
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• pp.346-351
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• 2022

This paper is to propose a curriculum of a subject of Enneagram which is to be open to develop key competency of university students. In the modern days of the 4th industrial revolution universities are being called for key competency-based education and innovation in education. Universities suggested various key competencies according to the university's founding philosophies. And they have been reorganizing the liberal arts curriculum in a way that it can reinforce key competencies of the students in the aspect of liberal arts education. In this paper, an example of C university which has 3 key competencies such as, citizenship, character, and creative intelligence was presented. Enneagram is a good candidate for developing self-development which is sub-competency of character.

• Current Optics and Photonics
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• v.7 no.4
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• pp.463-470
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• 2023

Hydroxygraphene as a kind of functionalized graphene has important applications in composite, photoelectric and biological materials. In the present study, THz and microfluidic technologies were implemented to study the THz transmission characteristics of hydroxygraphene with different concentrations and residence times in magnetic and electric fields. The results show that the THz transmission intensity decreases with the increase in sample concentration and duration of an applied electric field, while it increases by staying longer in the magnetic field. The phenomenon is analyzed and explained in terms of hydrogen bond, conductivity and scattering characteristics. The results establish a foundation for future research on the THz absorption characteristics of liquid graphene based on microfluidic technology in different external environments. It also provides technical support for the application and development of graphene in THz devices.

• Geomechanics and Engineering
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• v.22 no.6
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• pp.529-540
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• 2020

Water inrush is a major hazard for mining and excavation in deep coal seams or rock masses. It can be attributed to the coalescence of rock fractures in rock mass due to the interaction of fractures, hydraulic flow and stress field. One of the key technical challenges is to understand the course and mechanism of fluid flows in rock joint networks and fracture propagation and hence to take measures to prevent the formation of water inrush channels caused by possible rock fracturing. Several case observations of fluid flowing in rock joint networks and coupled fracture propagation in underground coal roadways are shown in this paper. A number of numerical simulations were done using the recently developed flow coupling function in FRACOD which simulates explicitly the fracture initiation and propagation process. The study has demonstrated that the shortest path between the inlet and outlet in joint networks will become a larger fluid flow channel and those fractures nearest to the water source and the working faces become the main channel of water inrush. The fractures deeper into the rib are mostly caused by shearing, and slipping fractures coalesce with the joint, which connects the water source and eventually forming a water inrush channel.

• Journal of Microbiology and Biotechnology
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• v.30 no.8
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• pp.1252-1260
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• 2020

(R)-2-(4-hydroxyphenoxy)propionic acid (HPOPA) is a key intermediate for the preparation of aryloxyphenoxypropionic acid herbicides (R-isomer). In order to improve the HPOPA production from the substrate (R)-2-phenoxypropionic acid (POPA) with Beauveria bassiana CCN-A7, static cultivation and H₂O₂ addition were attempted and found to be conducive to the task at hand. This is the first report on HPOPA production under static cultivation and reactive oxygen species (ROS) induction. On this premise, the cultivation conditions and fermentation medium compositions were optimized. As a result, the optimal carbon source, organic nitrogen source, and inorganic nitrogen source were determined to be glucose, peptone, and ammonium sulfate, respectively. The optimal inoculum size and fermentation temperature were 13.3% and 28℃, respectively. The significant factors including glucose, peptone, and H₂O₂, identified based on Plackett-Burman design, were further optimized through Central Composite Design (CCD). The optimal concentrations were as follows: glucose 38.81 g/l, peptone 7.28 g/l, and H₂O₂ 1.08 g/l/100 ml. Under the optimized conditions, HPOPA titer was improved from 9.60 g/l to 19.53 g/l, representing an increase of 2.03-fold. The results obtained in this work will provide novel strategies for improving the biosynthesis of hydroxy aromatics.

• Steel and Composite Structures
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• v.23 no.2
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• pp.187-194
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• 2017

To reuse a broken plain concrete (PC) arch, a retrofitting method was proposed to ensure excellent structural performances, in which carbon fiber reinforced polymers (CFRPs) were applied to repair and strengthen the damaged PC arch through bonding and wrapping techniques. Experiments were carried out to reveal the deformation and the load carrying capacity of the retrofitted composite arch. Based on the experiments, repairing and strengthening effects of the CFRP retrofitted broken arch were revealed. Simplified analysing model was suggested to predict the peak load of the CFRP retrofitted broken arch. According to the research, it is confirmed that absolutely broken PC arch can be completely repaired and reinforced, and even behaves more excellent than the intact PC arch when bonded together and strengthened with CFRP sheets. Using CFRP bonding/wrapping technique a novel efficient composite PC arch structure can be constructed, the comparison between rebar reinforced concrete (RC) arch and composite PC arch reveals that CFRP reinforcements can replace the function of steel bars in concrete arch.