• Biomolecules & Therapeutics
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• v.14 no.2
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• pp.114-118
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• 2006

Depression is a very difficult disease to be cured because several nervous systems are involved. In the present study, we evaluated the effects of Keum-Ryung-Ja-San (KRJS), a traditional herbal prescription, on depressive behaviors in mice using the forced swimming test. KRJS was given 1 h prior to the forced swimming test (50, 100, 200, an400 mg/kg, p.o.). The duration of immobility time in the forced swimming test was significantly reduced by KRJS treatment (200 mg/kg, P<0.05) and similar effects were observed with a classical antidepressant, imipramine (15 mg/kg, i.p.). With subchronic administrations of KRJS and its constituents at several doses for 1 week, a decreased duration of immobility time was observed with KRJS and Corydalis ternata (200 mg/kg, p.o. P<0.05). These results suggest that KRJS may have antidedpressive activities and CT may contribute to the antidepressive activity of KRJS.

• The Korea Journal of Herbology
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• v.25 no.2
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• pp.89-98
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• 2010

Objective : The aim of this study was to determine whether methanol extract of Keum-Ryung-Ja-San (KRJS) inhibit production of NO, $PGE_2$, iNOS, COX-2 and pro-inflammatory cytokines in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages. Methods : Cytotoxic activity of extracts on RAW 264.7 cells was measured using 5-(3-caroboxymeth-oxyphenyl)-2H-tetra-zolium inner salt (MTS) assay. The nitric oxide (NO) production was measured by Griess reagent system. And proinflammatory cytokines and PGE2 were measured by ELISA kit. The levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2(COX-2), $I{\kappa}-B-\alpha$ and nuclear NF-${\kappa}B$ p65 expression were detected by western blot. Results : Our results indicated that methanol extract of KRJS significantly inhibited the LPS-induced NO, $PGE_2$ production and iNOS, COX-2 expression accompanied by an attenuation of TNF-$\alpha$, IL-$1{\beta}$ and IL-6 production in RAW 264.7 cells. Moreover, methanol extract of KRJS treatment also blocked LPS-induced NF-${\kappa}B$ activation. Conclusion : These findings indicate that methanol extract of KRJS inhibits the production of pro-inflammatory mediators and cytokines via suppression of NF-${\kappa}B$ activation. Take together, these results indicate that methanol extract of KRJS has the potential for use as an agent of anti-chronic inflammatory diseases.