• Journal of Animal Reproduction and Biotechnology
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• v.34 no.4
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• pp.280-291
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• 2019

Potential utility of 14 candidate housekeeping genes as normalization reference for RT-qPCR analysis with developmental samples (fertilized eggs to late veliger larvae) in Pacific abalone Haliotis discus hannai was evaluated using four different statistical algorithms (geNorm, NormFinder, BestKeeper and comparative ΔCT method). Different algorithms identified different genes as the best candidates, and geometric mean-based final ranking from the most to the least stable expression was as follow: RPL5, RPL4, RPS18, RPL8, RPL7, UBE2, RPL7A, GAPDH, RPL36, PPIB, EF1A, ACTB and B-TU. The findings were further validated via relative quantification of metallothionein (MT) transcripts using the stable and unstable reference genes, and expression levels of MT were greatly influenced according to the choice of reference genes. In overall, our data suggest that RPL5 and RPS18, either singly or in combination, are appropriate for normalizing gene expression in developmental samples of this abalone species, whereas ACTB, B-TU and EF1A are less stable and not recommended. In addition, our findings propose that standard deviations in geometric ranking as well as geometric mean itself should also be taken into account for the final selection of reference gene(s). This study could be a useful basis to facilitate the generation of accurate and reliable RT-qPCR data with developmental samples in this abalone species.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.51 no.1
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• pp.95-106
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• 2018

To evaluate appropriate reference genes for the normalization of quantitative reverse transcription PCR (RT-qPCR) data with embryonic and larval samples from Russian sturgeon Acipenser gueldenstaedtii, the expression stability of eight candidate housekeeping genes, including beta-actin (ACTB), elongation factor-1A (EF1A), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), histone 2A (H2A), ribosomal protein L5 (RPL5), ribosomal protein L7 (RPL7), succinate dehydrogenase (SDHA), and ubiquitin-conjugating enzyme E2 (UBE2A), were tested using embryonic samples from 12 developmental stages and larval samples from 11 ontogenic stages. Based on the stability rankings from three statistic software packages, geNorm, NormFinder, and BestKeeper, the expression stability of the embryonic subset was ranked as UBE2A>H2A>SDHA>GAPDH>RPL5>EF1A>ACTB>RPL7. On the other hand, the ranking in the larval subset was determined as UBE2A>GAPDH>SDHA>RPL5>RPL7>H2A>EF1A>AC TB. When the two subsets were combined, the overall ranking was UBE2A>SDHA>H2A>RPL5>GAPDH>EF1A>ACTB>RPL7. Taken together, our data suggest that UBE2A and SDHA are recommended as suitable references for developmental and ontogenic samples of this sturgeon species, whereas traditional housekeepers such as ACTB and GAPDH may not be suitable candidates.

• Journal of the Korean Society for Library and Information Science
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• v.25
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• pp.265-294
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• 1993

At the present. one of the very important functions of the information center or library is to provide information service on time and to the right person. But it is impossible if the shape of the material is not complete. So it is important to keep the material of information complete. This study is on deacidification of paper materials for enhancing useful life of information. This study can be summerized as follews; The material needs deacidification is all of machine-made paper except neutral paper, and hand made paper which are below pH 6.5. The best way for the deacidification for the small amount of materals is to use aqueous deacidifiers such as Calcium Carbonate, Magnesuim Carbonate and Borax. For mass deacidification process, Methoxy Magnesuim Methyle Carbonate and Magnesuim Oxide, called

• Journal of Fashion Business
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• v.12 no.6
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• pp.107-123
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• 2008

The purpose of this research is to compare and analyze the brassiere patterns sold in Shanghai and Korea and to present basic materials needed in developing the brassiere patterns in Chinese adult women market. The study subject which is to find the difference in brassiere patterns of Korea and China has been selected of 3 Korean brands and 4 Chinese brands. 1. For the brassiere patterns sold in Korea, it were using the same size for the width of Hook&Eye, space between the shoulder lace on the back, slope of wing and the location of the Keeper was similar. 2. For the cup supporting base, Chinese brassiere covers the lower part of the breast and as it is not comfortable and has a phenomenon of loosening up, there were severe puckering. For issues in the wearing experiment, amendments in patterns with the cup part was required. 3. Chinese brassieres were big differences in wing angles and for Aimer, it had the steepest trend in wings and thus the wings headed down and for Gujin, as the angle of the wing was smaller than $90^{\circ}$, it was heading upward. 4. Upon comparing the patterns of the Korean and Chinese brassiere, although most of the sizes excluding the wing angle had similar sizes, there was big differences in the wing angle. The reason why the brassiere patterns sold in China had bigger wing angle was due to the fact that more Chinese women had sway back body type than the Korean women and should reflect such difference in body size into making the brassiere patterns for each women.

• Proceedings of the Korea Technology Innovation Society Conference
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• 2017.11a
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• pp.515-534
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• 2017

본 연구는 Kingdon의 다중흐름모형(MSF: Multiple Stream Framework)을 통하여 정부연구개발예산 중 미래성장동력 부문에 대한 예산결정 과정을 살펴보았다. 연구 대상은 대통령 의지로 의제가 발기된 정책의제 국가전략프로젝트이며 비합리적 의사결정에 대한 견제수단으로 활용되는 예비타당성조사가 예산의 결정단계로 이어지는 일련의 과정을 살펴보았다. 연구의의는 3개 흐름에서 행해지는 비합리적 의사결정 케이스를 확인하고, 정부 R&D 사업의 예산결정과정에서 온-오프 스위치 역할을 하고 있는 게이트 키퍼(gate keeper)인 사업 평가자(PM), 구체적 사업계획안을 만드는 부처의 사업기획자, 그리고 자문역할을 하는 전문가집단을 정책혁신가로 바라볼 수 있다는 의의를 발견했다. 공공부문의 정책혁신가는 사업을 기획한 부처와 같이 자기(부처)이익을 추구하지는 않지만 공공가치를 추구하는 독립적인 위치에 있었고, 어느 일면에는 과학기술분야의 성장과 발전을 바라고 있었다. 다시 말해, 연구는 정책혁신가라는 존재가 특정한 개인 또는 집단일 뿐만 아니라 2개 집단 이상의 복수의 정책혁신가가 있을 수 있는 가능성을 확인하였다. 특히, 예비타당성조사에서 PM이 흐름 간의 중개를 상향적 직접적으로 수행하지 않지만, 하향적으로 사업기획자 등 주체들 간 의견을 조정하고 합의를 이끌어 내는 모습은 중요 활동을 하고 있음이 확인되었다. 연구는 정책혁신가의 새로운 형태를 발견하였지만, 정책결정의 전 주기가 아닌 정부예산 이후 국회예산이라는 특정 시기를 대상으로 하였는데, 분석 과정에서 예산과 시간의 제약으로 사업기획을 담당했던 부처 및 기재부공무원, 해당기술 분야 연구자, 국회 관계자 등 관련 주체들의 견해를 고루 담아내지 못한 아쉬움이 있다. 향후 다양한 참여자들의 의견을 수렴할 수 있다면 정책 결정 과정에 정책혁신가의 다층적(Multiple) 존재 여부와 특성을 면밀히 들여다 볼 수 있을 것이다.

• Journal of Korean Society of Archives and Records Management
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• v.17 no.4
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• pp.155-175
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• 2017

This study explores the present state of the National Archives of Korea (NAK) and the branch archives and proposes development programs to reestablish their roles. With regard to the NAK, I discussed five issues, and for the branch archives, I discussed three. The issues on NAK are as follows: 1) Is NAK the nation's record keeper?; 2) Does NAK promote the establishment of local archives?; 3) Does NAK supervise and oversee the records management of all public institutions?; 4) What is the raison $d^{\prime}{\hat{e}}tre$ and the vision of NAK?; and 5) Does NAK perform a simple administrative service? Meanwhile, the issues on the branch archives are as follows: 1) Is the branch archives a permanent records management institution?; 2) Are the names of the branch archives appropriate?; and 3) Are the branch archives simple document warehouses?

• Korea Journal of Hospital Management
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• v.25 no.1
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• pp.13-20
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• 2020

Purposes: This study has the purpose to the improvement of health promotion for local residents through delivery of high-quality medical service by improving imbalance of medical use and seeking an improvement plan for accessibility of effective medical service by understanding the pattern of medical use by region. Methodology: As for the method, this study derived results at the significance level of p<0.05 through chi square test(χ² test) and Generalized Estimating Equation(GEE) SAS 9.4 version by using the data of the 7th Korean Longitudinal Study of Ageing 2018. Findings: Study results show that local residents use medical service such as hospitalization care and outpatient visit more as compared to the residents in Gyeonggi-do/large or medium-sized cities. The more the number of chronic disease, the more they select hospitalization care rather than outpatient visit. Results also show that patients engaged in labour tend to select outpatient treatment rather than hospitalization treatment. Meanwhile, severity of disease turned out to be higher amongst medical care beneficiaries than that of national health insurance patients when comparing the types of medical security. Practical Implications: In stead of solving the problem of the difference in medical use by region from the aspect of income and economic level, an integrative solution shall be provided putting viewpoint on the social phenomenon suited to the changes of the times. This study suggests a plan for using a health and medical community care center that acts as a gate keeper of regional medical service.

• The Journal of Advanced Prosthodontics
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• v.8 no.1
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• pp.9-15
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• 2016

PURPOSE. Although magnetic attachment is used frequently for overdenture, it is reported that attractive force can be decreased by abrasion and corrosion. The purpose of this study was to establish the clinical basis about considerations and long term prognosis of overdenture using magnetic attachments by investigating the change in attractive force of magnetic attachment applied to the patients. MATERIALS AND METHODS. Among the patients treated with overdenture using magnetic attachments in Dankook University Dental Hospital, attractive force records of 61 magnetic attachments of 20 subjects who re-visited from July 2013 to June 2014 were analyzed. Dental magnet tester (Aichi Micro Intelligent Co., Aichi, Japan) was used for measurement. The magnetic attachments used in this study were Magfit IP-B Flat, Magfit DX400, Magfit DX600 and Magfit DX800 (Aichi Steel Co., Aichi, Japan) filled with Neodymium (NdFeB), a rare-earth magnet. RESULTS. Reduction ratio of attractive force had no significant correlation with conditional variables to which attachments were applied, and was higher when the maintenance period was longer (P<.05, r=.361). Reduction ratio of attractive force was significantly higher in the subject group in which attachments were used over 9 years than within 9 years (P<.05). Furthermore, 16.39% of total magnetic attachments showed detachment of keeper or assembly. CONCLUSION. Attractive force of magnetic attachment is maintained regardless of conditional variables and reduction ratio increased as the maintenance period became longer. Further study on adhesive material, attachment method and design improvement to prevent detachment of magnetic attachment is needed.

• Journal of the Korean Institute of Landscape Architecture
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• v.23 no.1
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• pp.51-65
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• 1995

Conservation of the natural environiment In chosun-Dynasty, Korea, is analysed on the basis of the of official records of the king's offices(Chosun-wangjo-sillok). In the popular ideologies of the dynasty, Seongleehak(philosophy of humanity arid natural laws) and Pungsu (geomancy) , the naturnal enviroment is defined as an entity con-sisting of Cheon (heaven) , San(mnountains) , Su (water), Geumsu (animals)and Chomok (plants) .the notion of Tacksi-Tackmul(being careful to take natural resources at the right time) was one of the Cheonmyung (eavenly decrees) . It was believed that violation of this principle resulted in natural disasters. Sasan(four mountains surrounding Hansungbu-, the capital city, now Seoul) , were strictly preserved according to geomancy practices. In areas other than the capital city, Sanlimcheontack (mountains, forests, rivers and ponds) were con-served for sustained production of building materials especially pinetrees for ship building, foods, firewoods, horses, and orter useful natural resources. Various conservation policy insturuments were adopted. Prunning trees was permitted only in October. Capturing fishes was allowed only after the spawning period. Protection areas were designated in Sasan, Seongjoesiplee (surroundings of the capital city) , Geumsan(protected mountains), Kangmujang(hunting and army training grounds), Sijang(firewood areas), Mokmajang(horse ranches) and Neungyuk(royal tombs) . Activities prohibited for conservation purposes included cutting timbers, burning, building houses or tombs, dumping wastes, farming and breaking up fresh land, grazing, hunting and trespassing. Positive actions for conservation were rituals to Heaven ennoblement of natural elements such as mountians or rivers, planting trees, Boto (supplementing soil on low ridges) , Josan (mounding) and making ponds. Boto, Josan and making ponds were to make ideal terrains for geomancy. Many government bodies and civil servants were engaged in the conservation activities. For example, Sanjik(forest keeper) was a special position with responsibility for forest management.

• Journal of Korean society of Dental Hygiene
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• v.6 no.4
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• pp.403-417
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• 2006

The purpose of this study is that level of community members about dental hygienist's job of cognition and perception. The subjects of this survey were 500 of community members located in Gumi, Seoul, Incheon, Wonju.(response rate 92%) The results of study could be summarized as follows: 1. Women, 30 age group, officer and house keeper who had were experienced scaling higher level cognition of dental hygienist than another groups. 2. The group that had experienced oral examination, oral health consult, and the young group who had not experienced prosthodontic treatment had higher perception that dental hygienist main role is oral health care education. 3. The student who had experienced scaling had higher perception that dental hygienist's main role is oral disease prevention. 4. Over 30 age, women group who had experienced dental treatment between 6 month and 2 years had higher perception that dental hygienist main role is dental assistance At the conclusion of this investigation, We investigated that person's perception and cognition is promoted through scaling. Therefore we have to increase opportunity of oral health prevention and education role. In addition, we should notify dental hygienist with uniform, name tag and information about dental hygienist.