• Asian Pacific Journal of Cancer Prevention
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• v.16 no.3
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• pp.1241-1245
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• 2015

Background: Thyroid carcinoma is the most common malignancy of the endocrine organs. Although the majority of thyroid cancer patients experience positive outcomes, anaplastic thyroid carcinoma is considered one of the most aggressive malignancies. Current therapeutic regimens do not confer a significant survival benefit, and new therapies are urgently needed. Oncolytic herpes simplex virus (oHSV) may represent a promising therapy for cancer. In the present study, we investigated the therapeutic effects of a third-generation HSV vector, $G47{\Delta}$, on various human thyroid carcinoma cell lines in vitro. Two subcutaneous (s.c.) models of anaplastic thyroid carcinoma were also established to evaluate the in vivo anti-tumor efficacy of $G47{\Delta}$. Materials and Methods: The human thyroid carcinoma cell line ARO, FRO, WRO, and KAT-5, were infected with $G47{\Delta}$ at different multiplicities of infection (MOIs) in vitro. The survival rates of infected cells were calculated each day. Two s.c. tumor models were established using ARO and FRO cells in Balb/c nude mice, which were intratumorally (i.t.) treated with either $G47{\Delta}$ or mock. Tumor volumes and mouse survival times were documented. Results: $G47{\Delta}$ was highly cytotoxic to different types of thyroid carcinomas. For ARO, FRO, and KAT-5, greater than 30% and 80% of cells were killed at MOI=0.01 and MOI=0.1, respectively on day 5. WRO cells displayed modest sensitivity to $G47{\Delta}$, with only 21% and 38% of cells killed. In the s.c. tumor model, both of the anaplastic thyroid carcinoma cell lines (ARO and FRO) were highly sensitive to $G47{\Delta}$; $G47{\Delta}$ significantly inhibited tumor growth and prolonged the survival of mice bearing s.c. ARO and FRO tumors. Conclusions: The oHSV $G47{\Delta}$ can effectively kill different types of human thyroid carcinomas in vitro. $G47{\Delta}$ significantly inhibited growth of anaplastic thyroid carcinoma in vivo and prolonged animal survival. Therefore, $G47{\Delta}$ may hold great promise for thyroid cancer patients.

• Journal of The Institute of Information and Telecommunication Facilities Engineering
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• v.5 no.1
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• pp.43-59
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• 2006

A compact and broadband $4\times1$ array antenna was developed for 3G smart antenna system testbed. The $4\times1$ uniform linear away antenna was designed to operate at 1.885 to 2.2GHz with a total bandwidth of 315MHz. The array elements were based on the novel broadband L-probe fed inverted hybrid E-H (LIEH) shaped microstrip patch, which offers 22% size reduction to the conventional rectangular microstrip patch antenna. For steering the antenna beam, a commercial variable attenuator (KAT1D04SA002), a variable phase shifter (KPH350SC00) with four units each, and the corporate 4-ways Wilkinson power divider which was fabricated in-house were integrated to form the beamforming feed network. The developed antenna has an impedance bandwidth of 17.32% $(VSWR\leq1.5)$, 21.78% $(VSWR\leq2)$ with respect to center frequency 2.02GHz and with an achievable gain of 11.9dBi. The design antenna offer a broadband, compact and mobile solution for a 3G smart antenna testbed to fully characterized the IMT-2000 radio specifications and system performances.

• Journal of The Institute of Information and Telecommunication Facilities Engineering
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• v.7 no.1
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• pp.41-58
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• 2007

A compact and broadband $4{\times}1$ array antenna was developed for 3G smart antenna system testbed. The $4{\times}1$ uniform linear array antenna was designed to operate at 1.885 to 2.2GHz with a total bandwidth of 315MHz. The array elements were based on the novel broadband L-probe fed inverted hybrid E-H (LIEH) shaped microstrip patch, which offers 22% size reduction to the conventional rectangular microstrip patch antenna. For steering the antenna beam, a commercial variable attenuator (KAT1D04SA002), a variable phase shifter (KPH350SC00) with four units each, and the corporate 4-ways Wilkinson power divider which was fabricated in-house were integrated to form the beamforming feed network. The developed antenna has an impedance bandwidth of 17.32% ($VSWR{\leq}1.5$), 21.78% ($VSWR{\leq}2$) with respect to center frequency 2.02GHz and with an achievable gain of 11.9dBi. The design antenna offer a broadband, compact and mobile solution for a 3G smart antenna testbed to fully characterized the IMT-2000 radio specifications and system performances.

• Journal of Microbiology
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• v.35 no.4
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• pp.277-283
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• 1997

Promoters inducible by paraquat, a superocide-generating agent, were isolated from Escherichia coli using a promoter-probing plasmid pRS415 with promoterless lacA gene. Twenty one promoters induced by paraquat were selected and further characterized. From sequence analysis, thirteen of the promoters were mapped to their specific loci on the Escherichia coli chromosome. Several promoters were mapped to the upstream of known genes such as usgl, katG, and mglB, whose relationships with superoxide response have not been previously reported. Other promoters were mapped to the upstream region of unknown open reading frames. Downstream of HC 96 promoter are uncharacterized ORFs whose sequences are homologous to ABC-transporter subunits. Downstream of HC84 promoter is an ORF encoding a transcriptional regulator-like protein, which contains a LysR family-specific HTH (helix-turn-helix) DNA bindign motif. We investigated whether these promoters belong to the soxRS regulon. All promoters except HC96 were found to belong to the soxRS regulon. The HC96 promoter was significantly induced by paraquat in the soxRS deletion mutant strain. The basal transcription level of three promoters (HE43, HC71, HD94) significantly increased at the stationary phase, implying that they are regulated by RpoS. However, paraquat inducibility of all promoters disappeared in the stationary phase, suggesting that SoxRS regulatory system is active only in rapidly growing cells.

• Physical Therapy Korea
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• v.15 no.1
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• pp.20-29
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• 2008

The purpose of this study was to assess the effect of a group self exercise program in improving the quality of life regarding depression and the activities of daily living (ADL) of chronic stroke survivors, as well as the motor functions such as the 3 meter round walk, upper extremity function, and static balance. The subjects were 12 post-stroke ambulatory community center participants. All subjects participated in one 90 minute session per week for 7 weeks and received a home exercise program in every session. They had to record and submit an exercise check list. Quality of life was measured with the Beck depression inventory and the 8-Item Short-Form (SF-8). Motor functions were measured with the manual function test (MF'T), the kinesthetic ability trainer (KAT 3000), and the modified Barthel index. The level of depression decreased somewhat, but there were no significant differences after intervention. However, quality of life related health (SF-8) improved significant1y. There were significant improvements in the time for the 3 meter round walk, the functions of the affected upper extremities, and static balance and ADL (p<.05). The findings of this study suggest that a group self exercise program can improve quality of life related health and motor functions in stroke survivors.

• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.6
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• pp.395-399
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• 2000

Five different freeze-dried recombinant bioluminescent bacteria were used for the detection of cellular stresses caused by endocrine disrupting chemicals. These strains were DPD2794 (recA::luxCDABE), which is sensitive to DNA damage, DPD2540 (fabA::luxCDABE), sensitive to cellular membrane damage, DPD2511 (katG::luxCDABE), sensitive to oxidative damage, and TV1061 (grpE::luxCDABE), sensitive to protein damage. GC2, which emits bioluminescence constitutively, was also used in this study. The toxicity of several chemicals was measured using GC2. Damage caused by known endocrine disrupting chemicals, such as nonyl phenol, bisphenol A, and styrene, was detected and classified according to toxicity mode, while others, such as phathalate and DDT, were not detected with the bacteria. These results suggest that endocrine disrupting chemicals are toxic in bacteria, and do not act via an estrogenic effect, and that toxicity monitoring and classification of some endocrine disrupting chemicals may be possible in the field using these freeze-dried recombinant bioluminescent bacteria.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.50 no.1
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• pp.35-40
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• 2024

Objectives: Oral carcinoma cuniculatum (OCC) is a rare variant of squamous cell carcinoma (SCC). It has similar clinicopathological characteristics to SCC and verrucous carcinoma (VC). We present a case series of OCC and analyse its unique features, diagnosis, and management. Patients and Methods: We retrospectively reviewed the medical records of oral cancer patients treated by Oral and Maxillofacial Surgery department from 2009 to 2020 with OCC biopsy findings. The clinicopathological characteristics and management of the OCC cases were analysed. Results: Four patients were identified with histologic findings of OCC, including three on the alveolar ridge mucosa and one on the tongue. Imaging revealed that two of the lesions located in the maxilla had osseous lysis. All four patients were all treated with radical excision, and the histopathology showed findings of SCC cuniculatum. It was decided that no further treatment was necessary. None of the patients has experienced recurrence during follow-up. Conclusion: OCC is a distinct entity that is more locally aggressive than VC but is associated with good prognosis. Radical surgical removal is considered appropriate for OCC. Emphasis should be given on an early diagnosis, as it remains challenging.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.2
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• pp.723-731
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• 2016

Background: Cancer is a major public health problem in many parts of the world. Gastrointestinal (GI) cancers are responsible for 20% of all cancer-related deaths. In Turkey, stomach cancers account for 8.9%, colon cancer for 6.9%, and pancreatic cancer for 5.9%. This study examined the anxiety-depression levels and the quality of life of patients with GI cancer. Materials and Methods: This descriptive study was carried out on 335 adult patients who had gastrointestinal cancer and who were hospitalized in medical oncology clinics. Data were collected by using hospital anxiety and depression scale, EORTC QLQ C-30 and a patient information form. Results: Patients who were male and secondary school graduates/graduates/postgraduates experienced more functional difficulties. Patients with poor economic status experienced more symptoms. Patient general wellbeing decreased with increase disease duration. The level of functional difficulties decreased with an increasing number of hospital stays. Anxiety scores increased with decreasing age. Both anxiety and depression scores increased with increasing disease duration. Patients who were female, single/widowed/divorced, and literate/elementary school graduates had higher anxiety and depression scores. Life quality decreased with increasing anxiety and depression. Conclusions: Patients should be supported to prevent anxiety and depression, and should be followed up with this in mind.

• Progress in Superconductivity and Cryogenics
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• v.8 no.2
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• pp.25-28
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• 2006

The magnet system of KSTAR(korean Superconducting Tokamak Advanced Research) is consisted of 16 TF (Toroidal Field) coils and 14 PF (Poroidal Field) coils. Internal cooling CICC(Cable in Conduit Conductor) type conductor is used for both of TF and PF coil systems. The conduit material for $Nb_3Sn$ cable is Incoloy 908 and 316LN stainless-steel was used as conduit material for NbTi cable. $Nb_3Sn$ CICC is used for all TF coils and PF1-5 coils while NbTi CICC is used for PF6 and 7 coils. $Nb_3Sn$ and NbTi strands were made for KSTAR superconducting strand. They are satisfied with KSTAR superconducotr requirements. The $Nb_3Sn$ strands supplied from three companies; MELCO (Mitsubishi Electric Co.), OAS (Outokumpu Advanced Superconductor) and KAT (Kiswire Advanced Technology) were used. A special CICC jacketing system is developed for the KSTAR CICC fabrication which uses the tube-mill process consisted of forming, welding, sizing and squaring procedures. The. procedures for cabling and jacketing of CICC for TF and PF coils and their results including the geometrical specification and characteristics of strands are described.

• Journal of Microbiology and Biotechnology
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• v.23 no.4
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• pp.451-458
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• 2013

The human gastric pathogen Helicobacter pylori (Hp) has been considered a microaerophile. However, we recently reported that, when supplied with 10% $CO_2$, Hp growth is stimulated by an atmospheric level of $O_2$, suggesting that Hp is a capnophilic aerobe. In this study, we investigated the effects of aerobic $O_2$ tension on Hp cells by comparing gene expression profiles of cultures grown under microaerobic and aerobic conditions in the presence of 10% $CO_2$. The results showed that overall differences in gene expression in Hp cells grown under the two $O_2$ conditions were predominantly growth-phase-dependent. At 6 h, numerous genes were down-regulated under the aerobic condition, accounting for our previous observation that Hp growth was retarded under this condition. At 36 h, however, diverse groups of genes involved in energy metabolism, cellular processes, transport, and cell envelope synthesis were highly up- or down-regulated under the aerobic condition, indicating a progression of the cultures from the log phase to the stationary phase. The expression of several oxidative stress-associated genes including tagD, katA, and rocF was induced in response to aerobic $O_2$ level, whereas trxA, trxB, and ahpC remained unchanged. Altogether, these data demonstrate that aerobic $O_2$ tension is not detrimental to Hp cells but stimulates Hp growth, supporting our previous finding that Hp may be an aerobic bacterium that requires a high $CO_2$ level for its growth.