• 제목/요약/키워드: KatA

검색결과 59건 처리시간 0.025초

역교잡반응법을 이용한 아이소니아지드 및 리팜피신 신속감수성검사 (Rapid Drug Susceptibility Testing for Isoniazid and Rifampicin by Reverse Hybridization Assay)

  • 박영길;유희경;류성원;배길한
    • Tuberculosis and Respiratory Diseases
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    • 제55권5호
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    • pp.440-448
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    • 2003
  • 연구배경 : 다제내성균의 신속한 확인을 가능케 해주는 감수성검사법은 신속한 처방결정을 통해 환자의 치료 성공률을 향상 시킴과 동시에 다제내성균의 전파를 조기에 차단할 수 있게 되어, 국가 결핵관리 사업의 효율성을 증대시킬 수 있다. 방 법 : 아이나 또는 리팜피신 내성에 관련된 유전자 rpoB, katG, inhA, ahpC 등의 돌연변이를 검출할 수 있는 probe를 합성하였고, 총 502개의 내성균을 대상으로 중함효소연쇄반응 역교잡반응법으로 내성균 검출을 시도하였다. 결 과 : 264개의 리팜피신 내성균 중에서 Ser531Leu돌연변이가 46%를 차지하였고, codon 526에서는 32% 의 균주에서 돌연변이를 보였으며, codon 516에서는 10%의 균주가 돌연변이를 나타냈다. 469개의 아이나 내성균 중에서는 64%가 katG 유전자의 Ser315Thr 돌연변이를 나타내었고, 19%의 균은 inhA 유전자 promoter 돌연변이를 가지고 있었으며, ahpC유전자 돌연변이는 3%에 불과하였다. 역교잡반응법에 의한 검출율은 아이나 내성균 중 80%이상이었으며, 리팜피신 내성균에서도 92%이상을 검출할 수 있었다. 결 론 : 역교잡반응법에 의해 리팜피신 뿐만 아니라 아이나에 대한 감수성검사를 신속하게 수행할 수 있음을 확인하였고, 이 검사법은 특히 재발 또는 다제내성이 의심되는 환자의 처방 결정에 매우 유용한 수단이 될 수 있다.

밤나무 잉크병균, Phytophthora katsurae의 검출을 위한 Duplex PCR (A Duplex PCR for Detection of Phytophthora katsurae Causing Chestnut Ink Disease)

  • 이동현;이선근;김혜정;이상현;이상용;이종규
    • 식물병연구
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    • 제18권2호
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    • pp.73-79
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    • 2012
  • Phytophthora katsurae는 밤나무 잉크병의 원인이 되는 병원성 균류이다. P. katsurae를 검출하기 위하여 2개의 duplex PCR primer set을 제작하였다(SOPC 1F/1R+KatI 3F/5R, SOPC 1-1F/1-1R+KatI 3F/5R). SOPC 1F/1R과 SOPC 1-1F/1-1R primer pair는 sequence characteristic amplification regions(SCAR)를 이용하여 제작하였고, KatI 3F/5R primer pair는 internal transcribed spacer(ITS) 부위로부터 제작하였다. 추출한 genomic DNA를 1 mg/ml에서 1 ng/ml까지 10배씩 순차적으로 희석하여 균사량에 따른 Duplex PCR의 민감도를 확인한 결과, 2개의 primer set은 각각 1 ${\mu}g/ml$와 100 ng/ml까지 검출이 가능하였다. 유주포자를 $1{\times}10^6$부터 $1{\times}10^2$ cells/ml까지 10배씩 순차적으로 희석하고 genomic DNA를 추출하여 P. katsurae의 유주포자량에 의한 검출 한계를 확인한 결과, 2개의 primer set 모두 $1{\times}10^5$ cells/ml까지 검출할 수 있었다. 각각의 primer set은 PCR 검출을 실시하였을 때 P. katsurae 균주에서만 PCR 산물이 증폭된 반면에, Phytophthora 16종에서는 P. katsurae에 특이적인 PCR 증폭산물이 생성되지 않았다. 따라서, 2개의 primer set을 이용한 Duplex PCR은 P. katsurae의 신속하고 효과적인 검출에 유용하게 활용될 수 있을 것이다.

Relationships between Teaching Professional Rank, Course Taking, Teaching Experience and Knowledge of Algebra for Teaching

  • Huang, Rongjin;Li, Yeping;Kulm, Gerald;Willson, Victor
    • 한국수학교육학회지시리즈D:수학교육연구
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    • 제18권2호
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    • pp.129-148
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    • 2014
  • In this study, we examined the relationships among years of teaching experience, professional rank, number of courses taken, and knowledge of algebra for teaching (KAT). 338 in-service and 376 pre-service secondary mathematics teachers in China completed a KAT questionnaire. Various statistical techniques were employed to examine these relationships. The pre-service participants teachers performed statistically significantly higher in advanced mathematics knowledge than their in-service counterparts. Among the inservice teachers, senior teachers had scored higher in school mathematics and teaching mathematics, compared with junior teachers. Yet participants' advanced mathematics knowledge decreased as their professional rank advanced or their teaching experience increased. The number of courses taken has significantly positive correlation with school mathematics knowledge and advanced mathematics knowledge. The implications of these findings for mathematics teacher education are discussed.

영월하동 고씨동굴과 난고 김삿갓 시선의 문학외적 학술평가 (Art and Science Revaluation on Literary Outwards of Kim SaKat related with Gossi Cave in Youngworl)

  • 소대화
    • 동굴
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    • 제79호
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    • pp.17-30
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    • 2007
  • 난고 김삿갓의 생애 :선생은 안동 김씨의 시조인 고건 개국공신 신평의 후예로 순조 7년(1807년) 3월 13일 경기도 양주군 회천면 회암리에서 부 안근과 모 함평 이씨 사이의 2남으로 출생하였고, 본명은 병연(炳淵)이고 자는 성심(性深) 호는 난고(蘭皐) 별호는 김삿갓 또는 김립(金笠)이다. 순조 11년(1811년) "홍경래의 난"이 일어났을 때 조부 김익순이 선천부사로 있으면서 홍경래에게 항복, 역적으로 몰려 폐족처분을 받아 가족이 영월로 옮겨와 은둔생활을 하였다. 이러한 생활 속에서도 모친은 자식에게 조부의 사연을 감추고 글을 가르쳤으며, 20세 되던 해 영월 동헌에서 백일장에 응시하여 조부를 비판하는 글로 장원이 되었다. 그 후에 조부라는 사실을 알고 자책과 통한을 이기지 못하여 22세에 집을 나서 방랑생활을 하면서 서민들의 애환을 시로 읊어 서민문학의 큰 틀을 마련하였다. 1863년 전라남도 화순군 동복에서 작고하여 그곳에 묘를 썼으며, 3년 후 둘째 아들 익균이 지금의 묘로 옮겨 모셨다. (난고선생 묘 옆에 서있는 안내문에서)

Molecular Analysis of Isoniazid-Resistance Related Genes of Mycobacterium tuberculosis Isolated from Korea

  • Hwang Joo Hwan;Jeong Eun Young;Choi Yeon Im;Bae Kiho;Song Taek Sun;Cho Sang-Nae;Lee Hyeyoung
    • 대한의생명과학회지
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    • 제11권4호
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    • pp.455-463
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    • 2005
  • Resistance to isoniazid (INH), which is one of the most important drugs in Mycobacterium tuberculosis chemotherapy, has been associated with mutations in genes encoding the mycobacterial catalse-peroxidase (katG), the enoyl acyl carrier protein (ACP) reductase (inhA), alkyl hydroperoxide reductase (ahpC), beta-ketoacyl acyl carrier protein synthase (kasA), and NADH dehydrogenase (ndh). In this study, we examined INH-resistance related genes in 50 INH-resistant and 24 INH-susceptible isolates by PCR-sequence analysis. In brief, mutations at the katG gene were found at codon 315 alone (2/50), at codon 463 alone (19/50), and both at 315 and 463 (29/50). However, while mutations at codon 315 were only detected in INH-resistant isolates, mutations at codon 463 were also detected in INH-susceptible isolates indicating mutations at 463 alone do not seem to confer resistance to INH. Similar to the case of katG 463, some of inhA mutations were also found among INH-susceptible isolates. For example, whereas mutations at 8 upstream of the start codon (UPS) and 15 UPS of the inhA gene were detected only in INH-resistant isolates, mutations at 101, 115, and 125 UPS were detected only in INH-susceptible isolates. Many different kinds of mutations were detected in INH­resistant isolates at ahpC, oxyR gene, and intergenic region of the oxyR-ahpC genes. Howerver, the mutations were not found oxyR and the intergenic regions in INH-susceptible isolates. No mutations were found at either kasA or at ndh gene among INH-resistant isolates. In conclusion, some of mutations such as katG 315, inhA promotor region, and oxyR-ahpC seem to be strongly related to INH-resistance. Currently we are developing a molecular diagnostic method based on these results.

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Pyrosequencing 분석법을 이용한 Rifampicin과 Isoniazid 결핵약제내성의 빠른 검사법 (Pyrosequencing Based Detection of Rifampicin or Isoniazid Resistant in Mycobacterium tuberculosis)

  • 오서영;김효빈;신민식;김진욱;박성휘
    • 대한임상검사과학회지
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    • 제41권1호
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    • pp.24-30
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    • 2009
  • Rifampicin (RIF) and isoniazid (INH) are the most important drug for the treatment of Mycobacterium tuberculosis. Mutations correlated to rifampicin and isoniazid-resistance have been detected in rpoB gene and katG gene, respectively. Of the rifampicin-resistant isolates, 90% showed mutations in rpoB gene at codon 507 to 533. Isoniazid-resistant isolates analysed had a mutation in katG at codon 315. The aim of this study is to develop a pyrosequencing-based approach for rapid detection of ripampin or isoniazid resistant M. tuberculosis based on characterization of all possible mutation in the target region. For this study, the DNA selected from 35 cases of MTB PCR positive clinical sample such as bronchial washing, sputum, and pleural fluid. RIF or INH resistant was analyzed by pyrosequencing data of rpoB and katG gene. 28 (80%) and 7 (20%) of 35 MTB PCR positive DNAs were occured rifampicin-sensitivity and resistant, respectively. For INH, 30 (85.7%) and 5 (14.5%) cases were detected isoniazid-sensitivity and resistant, respectively. When pyrosequencing analysis was compared with ABI sequencing analysis, both analysis were presented same result, but pyrosequencing analysis was more rapid than ABI sequencing analysis. In conclusion, we found that pyrosequencing technology offers high accuracy, specificity, short turn around time and a high throughput in detection of rifampicin or isoniazid resistance in M. tuberculosis.

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Halliwick 10 Point Program이 균형능력에 미치는 영향 (The Effect of Halliwick 10 Point Program on the Balance Control)

  • 송명환;손민균;김태열;황태연
    • 대한임상전기생리학회지
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    • 제1권2호
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    • pp.49-60
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    • 2003
  • The purpose of this study was to evaluate the effect of Halliwick 10 point program on the balance control. Fourteen undergraduate students participated in the experiment, and classified 7 experiment and 7 control group, randomly. Halliwick 10 point program was applied in the experiment group for 4 weeks. Balance index was measured using KAT 2000. Balance index were measured before, during(2 weeks), and after(4 weeks) the training for 4 weeks. 2-way repeated measures ANOVA was used to further distinguish between the groups. The following results were obtained; 1. The total balance index scores from a KAT were significantly decreased after 4 weeks compared with before the training passing of time. However there was no significant difference between experiment and control group. 2. The left and anterior shifting balance index scores from a KAT were significantly decreased after 4 weeks compared with before the training passing of time. However there was no significant difference between experiment and control group. These results lead us to the conclusion that the balance ability of women twenties increase as the water is higher than that of the ground applied Halliwick 10 point program of the water specific therapy province. Therefore, A further direction of this study will be to provide more evidence for Halliwick 10 point program in the water specific therapy.

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간척지 식물군락형성 과정에 관한 연구 -목포지방을 중심으로- (An Ecological Study on the Process of Plant Community Formation in Tidal Land)

  • 김철수
    • Journal of Plant Biology
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    • 제14권4호
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    • pp.27-33
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    • 1971
  • Information this research an attempt was made to investigate the plant community structure and the process of its formation in the tidal area surrounding Mokpo City the examined area included the stand in Sam-Hak Do wherein sands have inflowed, and the stand in Kat-Ba-Woo which has been left as tidal soil land. Two hundred stands were sampled by making 100m line transects 5m apart and establishing 30cm$\times$30cm quadrates 10m apart on each tape. These data were used to establish frequency, cover degree, density, standing crop (g/$m^2$), contained Cl in the soil, and pH. Results of the research are as following: 1. There were 25 species of plants in the stands from Sam-Hak Do, 18 in the stands from Kat-Ba-Woo, and so 28 species in all. Regardings the importance value, the rank of dominant species is Salicornia herbacea L., Suaeda maritima Dumorties, Diplachne fusca L., Echinochola hispidula Nakai, Cyperus iria L., Setaria lutescens Hubbard in Sam-Hak Do, and Suaeda maritima Dumorties, Echinochloa hispidula Nakai, Aster tripolium L., Scripus maritimus L., Salicornia herbacea L., Diplachne fusca L., in the lands from Kat-Ba-Woo. Among them are five kinds of halophytes Salicornia herbacea L., Suaeda maritima Dumorties, Atriplex Gmelini C.A. Meyer, Aster tripolium L., and Limonium tetragonum Bullock; and two Kinds of naturalized plants Diplachan fusca L., Aster subulata. M. In the stands from Sam-Hak Do there was evidence of a secondary succession phenomena in the presence of Cyperaceae such as Cyperus liria L., Juncus decipiens Nakai, and Fimbristylis longispica Steudal, which could not be found in the stands from Kat-Ba-Woo. 2. On the number of species of vegetation, the further inland from the floodgate the higher the number; that is, the lower the content of Cl the higher the number of species. 3. On the distribution of the vegetation; comparing DFD index and Cl content, the main plants are Salicornia herbacea L., Suaeda maritima Dumorties, Atriplex gmelini C.A. Meyer, Diplachne fuscal, Echinochola hispidula Nakai where the Cl content of soil is more than 13.2%. 4. On the standing crop; Salicornia herbacea L., which has high resistance to salt, was half the total was production of 1090/$m^2$ while Suaeda maritima Dumorties was 1/4, Diplachne fusca L. 1/8, and Echinochloa the least. Analyzing the result, the research indicates that the main factor in plant community formation in tidal land is tolerance for Cl, and that a plan for utilizing the halophytes which are abundant in tidal land should be devised.

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KAT8/MOF-Mediated Anti-Cancer Mechanism of Gemcitabine in Human Bladder Cancer Cells

  • Zhu, Huihui;Wang, Yong;Wei, Tao;Zhao, Xiaoming;Li, Fuqiang;Li, Yana;Wang, Fei;Cai, Yong;Jin, Jingji
    • Biomolecules & Therapeutics
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    • 제29권2호
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    • pp.184-194
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    • 2021
  • Histone acetylation is a well-characterized epigenetic modification controlled by histone acetyltransferases (HATs) and histone deacetylases (HDACs). Imbalanced histone acetylation has been observed in many primary cancers. Therefore, efforts have been made to find drugs or small molecules such as HDAC inhibitors that can revert acetylation levels to normal in cancer cells. We observed dose-dependent reduction in the endogenous and exogenous protein expression levels of KAT8 (also known as human MOF), a member of the MYST family of HATs, and its corresponding histone acetylation at H4K5, H4K8, and H4K16 in chemotherapy drug gemcitabine (GEM)-exposed T24 bladder cancer (BLCA) cells. Interestingly, the reduction in MOF and histone H4 acetylation was inversely proportional to GEM-induced γH2AX, an indicator of chemotherapy drug effectiveness. Furthermore, pGL4-MOF-Luc reporter activities were significantly inhibited by GEM, thereby suggesting that GEM utilizes an MOF-mediated anti-BLCA mechanism of action. In the CCK-8, wound healing assays and Transwell® experiments, the additive effects on cell proliferation and migration were observed in the presence of exogenous MOF and GEM. In addition, the promoted cell sensitivity to GEM by exogenous MOF in BLCA cells was confirmed using an Annexin V-FITC/PI assay. Taken together, our results provide the theoretical basis for elucidating the anti-BLCA mechanism of GEM.

Analysis of the Stress Effects of Endocrine Disrupting Chemicals (EDCs) on Escherichia coli

  • Kim, Yeon-Seok;Min, Ji-Ho;Hong, Han-Na;Park, Ji-Hyun;Park, Kyeong-Seo;Gu, Man-Bock
    • Journal of Microbiology and Biotechnology
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    • 제17권8호
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    • pp.1390-1393
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    • 2007
  • In this study, three of the representative EDCs, $17{\beta}$-estradiol, bisphenol A, and styrene, were employed to find their mode of toxic actions in E. coli. To accomplish this, four different stress response genes, recA, katG, fabA, and grpE genes, were used as a representative for DNA, oxidative, membrane, or protein damage, respectively. The expression levels of these four genes were quantified using a real-time RT-PCR after challenge with three different EDCs individually. Bisphenol A and styrene caused high-level expression of recA and katG genes, respectively, whereas $17{\beta}$-estradiol made no significant changes in expression of any of those genes. These results lead to the classification of the mode of toxic actions of EDCs on E. coli.