• Title/Summary/Keyword: Karyotypes

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A cytotaxonomic study of Allium (Alliaceae) sect. Sacculiferum in Korea (한국산 부추속 산부추절의 세포분류학적 연구)

  • Ko, Eun-Mi;Choi, Hyeok-Jae;Oh, Byoung-Un
    • Korean Journal of Plant Taxonomy
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    • v.39 no.3
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    • pp.170-180
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    • 2009
  • Somatic chromosome counts and karyotype analyses were carried out for eight taxa of Korean Allium sect. Sacculiferum. The basic chromosome number of sect. Sacculiferum was x = 8, and they could be cytologically divided into two groups, that is, a diploid group (2n = 2x = 16) containing A. thunbergii var. thunbergii, A. thunbergii var. deltoides, A. thunbergii var. teretifistulosum, A. deltoidefistulosum, A. longistylum, A. linearifolium and A. taqueti, and a tetraploid group (2n = 4x = 32) with only A. sacculiferum. All observed chromosomes were classified into metacentric, submetacentric and subtelocentric. The metacentric ones appeared in all treated taxa. One or two pairs of submetacentric chromosomes were observed in most taxa except A. sacculiferum, the unique taxon with subtelocentric chromosomes. All taxa had a pair of homologous chromosomes with satellites, and the B-chromosomes found in A. thunbergii var. thunbergii, A. deltoidefistulosum, A. sacculiferum and A. longistylum, were metacentric or telocentric. The karyotypes of A. longistylum and A. linearifolium were firstly investigated in this study. In conclusion, the somatic chromosome numbers and karyotypes for members of the sect. Sacculiferum were valuable characters in identifying taxa, investigating interspecific relationships and delimiting taxa. In addition, A. thunbergii var. teretifolium, an invalid name (homonym), was renamed as A. thunbergii var. teretifistulosum H. J. Choi & B. U. Oh.

A Cytogenetic Study in Patients Referred for Suspected Chromosomal Abnormalities (염색체 이상 환자의 세포 유전학적 연구)

  • Oh, S.K.;Lee, H.H.;Moon, S.Y.;Chang, Y.S.
    • Clinical and Experimental Reproductive Medicine
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    • v.12 no.2
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    • pp.39-57
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    • 1985
  • Presented in this paper the data from a chromosome study of 397 patients referred for suspected chromosmal abnormalities. Karyotypes were obtained using short-term blood culture and direct method. Of these 238 patients had normal chromosome complements; 159 (40.1%) patients had chromosome abnormality. Among all patients with chromosome abnormalities, 82.4% (131/159) had aberrations of chromosome number, the others 17.60/0 (28/159\ had aberrations of chromosome structure. Ten had a chromosome rearrangement; Five of them were reciprocal and five Robertsonian translocations. Four patients with pericentric inversions and one with paracentric inversions and four with isochromosomes were observed. There were four patients with marker chromosome, two patients had a chromosome insertion; and three others. (additional abnormal chromosomes.) Thus the results of the present study indicate the importance of cytogenetic evaluation in clinically abnormal patients.

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Karyotype Analysis of Tobacco Moth, Ephestia elutezza H. (Lepidoptera : Pyralidae) and Cigarette Beetle, Lasioderma serricorne F. (Coleoptera : Anobiidae) (다색알락명나방(Ephestia elutella H.)과 궐련벌레(Lasioderma serricorne F.)의 핵형 분석)

  • Chae, Soon-Yong;Kim, Sang-Seock;Chang, Young-Dock
    • Journal of the Korean Society of Tobacco Science
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    • v.18 no.1
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    • pp.30-38
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    • 1996
  • Meiotic metaphase chromosomes of the testis of two storage insects, tobacco moth (Ephestia elutella H.) and cigarette beetle (Lasioderma serricorne F.) were examined to study their karyotypes. The number of haploid chromosomes of the tobacco moth was 31 and the karyotype was characterized by 29 metacentric and 2 submetacentric chromosomes. The metaphase chromosome length of tobacco moth ranged ca. 1.44-2.11${\mu}{\textrm}{m}$ and the average length was 1, 78${\mu}{\textrm}{m}$. The number of haploid chromosomes of the cigarette beetle at metaphase was 10 and the karyotype was characterized by 8 metacentric and 2 submetacentric chromosomes. The range of metaphase chromosome length of the cigarette beetle was ca. 1.79- 2.39${\mu}{\textrm}{m}$ and the average length 2.09${\mu}{\textrm}{m}$.

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Chromosomal Abnormalities in Pakistani Children with Acute Lymphoblastic Leukemia

  • Shaikh, Muhammad Shariq;Ali, Syed Sarwer;Khurshid, Mohammad;Fadoo, Zehra
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.9
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    • pp.3907-3909
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    • 2014
  • Background: Cytogenetic abnormalities have important implications in diagnosis and prognosis of acute leukemia and are now considered an important part of the diagnostic workup at presentation. Karyotype, if known at the time of diagnosis, guides physicians to plan appropriate management strategies for their patients. Aim and Objectives: To determine the cytogenetic profile of acute lymphoblastic leukemia (ALL) in Pakistani children in order to have insights regarding behavior of the disease. Materials and Methods: A retrospective analysis of all the cases of ALL (<15years old) diagnosed at Aga Khan University from January 2006 to June 2011 was performed. Cytogenetic analysis was made for all cases using the trypsin-Giemsa banding technique. Karyotypes were interpreted using the International System for Human Cytogenetic Nomenclature (ISCN) criteria. Results: A total of 153 patients were diagnosed as ALL during the study period, of which 127 samples successfully yielded metaphase chromosomes. The male to female ratio was 1.8:1. A normal karyotype was present in 51.2% (n=65) of the cases whereas 48.8% (n=62) had an abnormal karyotype. Most of the abnormal cases showed hyperdiploidy(13.4%) followed by t(9;22)(q34;q11.2) (7.08%). Conclusions: This study revealed a relative lack of good prognostic cytogenetic aberrations in Pakistani children with ALL.

Karyotypes of Genus Liobagrus (Pisces : Amblycipitidae) in Korea (한국산(韓國産) 퉁가리속(屬) 어류(魚類)의 핵형(核型) 분석(分析))

  • Son, Yeong-Mok;Lee, Ji-Hyun
    • Korean Journal of Ichthyology
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    • v.1 no.1_2
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    • pp.64-72
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    • 1989
  • Karyological characteristics were investigated in 3 species of the genus Liobagrus from Korea. The diploid chromosome number in L. andersoni was found to be 28, with 9 pairs of metacentrics and 5 pairs of submetacentric chromosomes, and arm number (AN) was 56. L. mediadiposalis was found with 2n of 42, consisting of 13 pairs of metacentrics and 8 pairs of submetacentric chromosomes (AN=84). In the case of L. obesus 2n was 20, with 20 metacentric chromosomes (AN=40), which was the lowest among the species of the order Siluriformes. Sexual dimorphism or intraspecific polymorphism of the chromosomes was not observed in any species examined.

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한국산 관박쥐(Rhinoiophus terrumequinum)의 핵형분석에 의한 분류학적 연구

  • 이정훈;손함원
    • The Korean Journal of Zoology
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    • v.31 no.4
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    • pp.309-317
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    • 1988
  • 내륙산 관박쥐와 제주산 관박쥐간의 동-종 여부를 조사하기 위하여 경남과 제주에서 채집한 이들 관박쥐들을 핵형분석하였다. 핵형분석의 결과, 2종 모두 염색체수는 2n=58이었으며 FN도 62였다. 상염색체에서는 중형의 2쌍과 미세형 1쌍이 중부염색체(metacentrics)였고, 대형에서 소형까지는 25쌍이 단부염색체facrocentrics)이었다. 성염색체에서 X염색체는 대형의 차중부염색체(submetacentrics)였고, Y염색체는 소형의 단부염색체(acrocentrics)이었다. 그리고 이들 염색체들은 동원체 부근에 이질염색체을 가지는 특이한 1쌍의 단부염색체(acrocentrics)가 존재하고 있었다. in this study, We analysed the karyotypes of the inland bat(Rhinolox)thus femequinum hora) and the Cheju-Island bat(Rhinorophus ferrumequinum quelpartis(\ulcorner)) collected in Kyungnam and Cheju provinces to identify the homogeneous between them. The results are as follows. The diploid number of chromosomes of them are equally 58 and the fundamental number 62. In the autosomes, metacentrics consist of two pairs of the middle form and a pair of the micro-form. And acrocentrics have 25 pairs of large and small form. In sex-chromosomes, X-chromosome is a large submetacentrics and Y is a small acrocentrics. And, these chromosomes possess a pair of particular acrocentrics having heterochromatin around centromere in both the inland bat(Rhinolophus fenmequinum korai ) and Cheju-Island bat(Rhinolophus fewmequinum querporis (\ulcorner)).

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갈겨니 (Zacco temmincki)의 進化에 관한 硏究 II: 갈겨니 2型의 核型分析

  • 이혜영;조정우;양서영
    • The Korean Journal of Zoology
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    • v.29 no.3
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    • pp.208-214
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    • 1986
  • Comparative study of karyotypes in two allotypes $(Mdh-1^{MM} and Mdh-1^{MS})$ of the dark chub (Zacco temmincki) was examined. Both types had diploid number of 48 but the 7th chromosome was strikingly different between them. The chromosomes of $Mdh-1^{MM}$ type was consisted of 6 pairs of metacentrics, 6 pairs of submetacentrics, and 12 pairs of acrocentrics whereas the chromosomes of $Mdh-1^{MS}$ type had 7 pairs of metacentrics, 5 pairs of submetacentrics and 12 pairs of acrocentrics. No hybrid type between these two types was found in sympatric area at Tongchon River Namhae. Probable reproductive isolation between them was discussed.

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Cytogenetic Analysis of Four Hosta Species Native to Korea

  • Kim, Hyun-Hee;Park, Young-Wook;Yoon, Pyung-Sub;Choi, Hae-Woon;Bang, Jae-Wook
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.5
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    • pp.397-400
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    • 2004
  • The chromosome numbers and karyotypes were investigated in four Korean native species of the genus Hosta. The chromosome complements were diploid of 2n=60 in H. japonica var. lancifolia Nakai and H. capitata Nakai, aneuploid of 2n=59 in H. minor (Bak.) Nakai, and modified triploid of 2n=92 in H. longipes (Fr. et Sav.) Matsumura. All the species carried four sets of distinctly large chromosomes of which the chromosome types were telocentrics or subtelocentrics with $4.4{\sim}7.2\;{\mu}m$ in length. The other chromosomes were meta-, submeta, subtelo-, or telocentric types and showed gradual length degradation in the range of $1.0{\sim}3.0\;{\mu}m$. The satellites appeared vestigially in a pair or a triplet set of chromosomes which depends on the species. New chromosome number and karyotype in H. longipes were the first report in this species. The structural rearrangement was suggested to explain the modified triploid composition of 2n=92.

Cytogenetic Studies of 384 Couples with Recurrent Abortion (반복유산을 경험한 384부부의 세포유전학적 연구)

  • Choi, Soo-Kyung;Min, Eung-Ki;Roh, Sung-Il;Paik, Yong-Kyun;Lyu, Myung-Soo
    • Clinical and Experimental Reproductive Medicine
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    • v.18 no.2
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    • pp.223-231
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    • 1991
  • During the years 1984 to 1989, in order to determine of chromosome abnormalities are associated with recurrent spontaneous abortions, cytogenetic studies were performed 384 couples. Abnormal karyotypes were found in 51(13.3%) couples. There was no apparent relation with the number of abortions. The abnormalities were as follows: 17(4.4%) balanced translocation; 15(3.9%) mosaicisms; 17(4.4%) pericentric inversion; 2(0.5%) addition or isochromosome. Chromosome abnormalities were observed in 34(67%) of the wives and 17(33%) of the husbands. In addition, we detected polymorphic variants of chromosomes in 89(23.2%) subjects. Reciprocal translocations(13/17) were more common than the robertsonian type(4/17). All of the mosaicisms were associated with the sex chromosomes in 10 females and 5 males subjects. Pericentric inversions were most common in chromosome 9. Compared to previously studied general populations, significantly higher frequencies of translocations, mosaicisms and inversions were found in couples with repetitive spontaneous abortion. This suggests that couples should have chromosome studies after two or more abortions.

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Interspecific Hybridization between Triploid Hybrid Fish, Cobitis sinensis - longicorpus and Two Diploid Species from Korea (잡종 3배체 어류 기름종개 - 왕종개 잡종군의 종간 교잡실험)

  • Kim, Ik-Soo;Lee, Eun-Hee
    • Korean Journal of Ichthyology
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    • v.7 no.1
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    • pp.71-78
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    • 1995
  • Cobitis sinensis - longicorpus complex were commonly found in the upper stream of the Nakdong River in Korea and consisted of mostly with diploid and triploid karyotype forms. Among them, the triploid females of C. sinensis - longicorpus were artificially crossed with diploid males from C. sinensis and diploid males from C. longicorpus, respectively. The progenies from each cross produced the diploid individuals that possessed the paternal characters in their karyotypes and their body color patterns. Based on this results, we can assume that the triploid females, C. sinensis - longicorpus have a peculiar reproductive mode eliminating an uneven genome by the primary meiotic division and then producing a haploid ovum by secondary meiosis.

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