• Title/Summary/Keyword: Karyotype analysis

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Karyotype of Dwarf Loach, Kichulchoia brevifasciata (Pisces: Cobitidae) from Korea (미꾸리과 소형어류 좀수수치, Kichulchoia brevifasciata의 핵형)

  • Kim, Ik-Soo;Kim, Eun-Jin
    • Korean Journal of Ichthyology
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    • v.20 no.1
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    • pp.61-65
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    • 2008
  • The karyotype analysis of Kichulchoia brevifasciata was carried out, known as an end-angered small endemic cobitid fish of Korea. Five females and one male were collected from Geum-san-myeon, Goheung-gun, Jeollanam-do, Korea and its gill, kidney and digestive tract tissues were treated by flame-drying technique. The karyotype was found to be 2n= 48 from 33 cells; 16 meta-submetacentric and 32 subtelo-telocentric chromosomes; 64 FN (fundamental number). This karyotype showed a remarkably different karyotype formula from Niwaella multifasciata which is closely related to this species.

Electrophoretic Karyotyping by PFGE in the Genus Fusarium (Fusarium속에서 PFGE를 이용한 Electrophoretic Karyotyping)

  • Min, Byung-Re;Jung, Jin-Sook;Choi, Yong-Keel
    • The Korean Journal of Mycology
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    • v.26 no.2 s.85
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    • pp.135-143
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    • 1998
  • Contour-clamped homogeneous electric field gel electrophoresis was used to establish electrophoretic karyotype for 10 species of Fusarium sections Sporotrichiella, Liseola, Gibbosum, Discolor and Martiella. Intact chromosomal DNA was isolated from fungal protoplast and separated under various conditions according to their size in order to improve DNA separation. The numbers of chromosome-sized DNA molecules for individual species ranged from 5-13, with individual chromosomes ranging from 0.78 Mb to 7.20 Mb in size. The total genome DNA size of each species was estimated at about 18.32 Mb to 48.20 Mb. Comparison of karyotype profiles following Southern hybridization analysis with a randomly selected genomic probe of F. oxysporum formae speciales litii was carried out.

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The Karyotype of a Riceash, Oryzias latipes from Southern Korea (한국 남부지방에 서식하는 송사리 Oryzias latipes (Temminck et Schlegel)의 핵형)

  • 김익수;문교정
    • The Korean Journal of Zoology
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    • v.30 no.4
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    • pp.379-386
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    • 1987
  • Karyotype of the ricessh Orygias latipes (Temminck et Schlegel) was investigated using 151 specimens of eleven populations in the southern Korea. Based on the karyotype analysis, two distinct chromosomal groups were found. One group, occurring in the Kum River, the Mankyong River, the Dongjin River, the Ysngsan River and the SEmjin River had a diploid chromosome number of 46 and arm number of 70, which contained one pair of "large" metacentric chromosomes. The other group, occurring in the Tamjin River, the Hyongsan River andthe all four island populations observed had a diploid chromosome number of 48 and arm number of 68. These results showed that 0. latipes of 6 inland populations was identical with the Chinese population in diploid number, arm number and presence of "large" metacentric chromosome, while the present species of island populations ana populations of the Tamjn R. and Hyongsan R. was similar to the Japanese population in chromosome number and arm number. The karyotype variation in populations indicated that karyotypes will probably provide useful information in studying zoogeography of this species.phy of this species.

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A Case of Two SRY Genes in a Tuner's Syndrome Feature (터너증후군 의심환자에서 2개의 SRY 유전자 발현 1예)

  • Park, Sang-Muk;Kim, Yoon-Sik
    • Korean Journal of Clinical Laboratory Science
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    • v.42 no.3
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    • pp.111-115
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    • 2010
  • A 15-year-old female with primary amenorrhea and Tuner's syndrome feature was referred for a chromosome analysis. The karyotype of the patient was 45,X/46,X,der(Y) mosaicism under initial GTG-banding analysis. Fluorescence in situ hybridization (FISH) analysis with probe for CEP X probes and SRY probe (Vysis, Inc. Downers Grove, IL 60515, USA) was carried out. This probe is direct labeled with SpectrumOrange (SRY, Yp11.3) and is available as a single probe or mixed with the CEP X SpectrumGreen probe. SRY SpectrumOrange/CEP X SpectrumGreen hybridized to a specimen obtained from an two isodicentric Y chromosomes. The karyotype of the patient was ish Xcen(DXZ1x1)/Xcen(DXZ1x1), Yp11.3(SRYx2) by using FISH. This karyotype was considered a variant of Tuner syndrome with mixed gonadal dysgenesis (MGD), male pseudohermaphroitism (MPH) and apparently normal male.

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Karyotype Analysis of Eight Korean Native Species in the Genus Iris

  • Kim, Hyun-Hee;Park, Young-Wook;Yoon, Pyung-Sub;Choi, Hae-Woon;Bang, Jae-Wook
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.5
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    • pp.401-405
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    • 2004
  • Karyotypes were established in the eight Korean native species of the genus Iris. Chromosome numbers were 2n=50 in I. koreana and 2n=42 in I. uniflora var. carinata and their karyotype formulas were K = 2n = 50 = 14m + 28sm + 8st and K = 2n = 42 = 16m + 26sm, respectively. I. dichotoma and I. pseudoacorus were diploids of 2n=34. However, they showed different karyotype formulas: K = 2n = 34 = 26m + 6sm + 2st in I. dichotoma and K = 2n = 34 = 8m + 24sm + 2st in I. pseudoacorus. I. setosa, and I. pallasii var. chinensis carried the same chromosome numbers of 2n=40, but they showed different patterns of karyotype formula: K = 2n = 40 = 22m + 14sm + 4st in I. setosa and K = 2n = 40 = 26m + 12sm + 2st in I. pallasii var. chinensis. I. sanguinea was a diploid of 2n=28 and the karyotype formula was K = 2n = 28 = 14m + 14sm. I. ensata var. spontanea was a diploid of 2n=24 and the karyotype formula was K = 2n = 24 = 10m + 14sm. Each species showed characteristic chromosome composition with a pair of satellite chromosome except I. koreana with three pairs of satellite chromosomes. The chromosomes of I. dichotoma and I. uniflora were comparatively short, while the chromosomes of I. ensata were remarkably bigger than those of other species. These cytological data will give a useful information for the identification and breeding program of the Iris plants.

Computing of the Fuzzy Membership Function for Karyotype Classification (핵형 분류를 위한 퍼지 멤버쉽 함수의 처리)

  • Eom, Sang-Hee;Nam, Jae-Hyun
    • Journal of the Korea Society of Computer and Information
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    • v.11 no.6 s.44
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    • pp.1-8
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    • 2006
  • Many researchers have been studied for the automatic chromosome karyotype classification and analysis. For the automatic classify the each chromosome which is the image in microscope, it is necessary to process the sub-procedure, ie. image pre-processing, implementing karyotype classifier. The image pre-processing proceeded the each chromosome separation, the noise exception and the feature parameter extraction. The extracted morphological feature parameter were the centromeric index(C.I.), the relative length ratio(R.L.), and the relative area ratio(R.A.). In this paper, the fuzzy classifier was implemented for the human chromosome karyotype classification. The extracted morphological feature parameter were used in the input parameter of fuzzy classifier. We studied about the selection of the membership function for the optimal fuzzy classifier in each chromosome groups.

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Esthesioneuroblastoma in a boy with 47, XYY karyotype

  • Jo, Hee Cheol;Lee, Seong Wook;Jung, Hyun Joo;Park, Jun Eun
    • Clinical and Experimental Pediatrics
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    • v.59 no.sup1
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    • pp.92-95
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    • 2016
  • Neuroblastomas are sometimes associated with abnormal constitutional karyotypes, but the XYY karyotype has been rarely described in neuroblastomas. Here, we report a case of an esthesioneuroblastoma in a boy with a 47, XYY karyotype. A 6-year-old boy was admitted to our hospital because of nasal obstruction and palpable cervical lymph node, which he first noticed several days previously. A polypoid mass in the right nasal cavity was detected through sinuscopy. Biopsy of the right nasal polyp was performed. Based on the result, the patient was diagnosed with a high-grade esthesioneuroblastoma. Nuclear imaging revealed increased uptake in both the right posterior nasal cavity and the right cervical IB-II space, suggesting metastatic lymph nodes. Cytogenetic analysis revealed a 47, XYY karyotype. Twelve courses of concurrent chemotherapy were administered. Three years after the completion of chemotherapy, the patient had had no disease recurrence. He manifested behavioral violence and temper tantrums, so we started methylphenidate for correction of the behavior.

Karyotype Analysis of Liobagrus somjinensis, an Endemic Species in Korea (한국 고유종 섬진자가사리 Liobagrus somjinensis의 핵형 분석)

  • Cho, Yun Jeong;Park, Jong Young
    • Korean Journal of Ichthyology
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    • v.28 no.3
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    • pp.175-178
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    • 2016
  • The karyotype analysis of Liobagrus somjinensis, an endemic Korean freshwater fish with restricted waters and a new species of torrent catfish was carried out from nine females and eight males of Somjingang River, Sikjeong-dong, Namwon-si, Jeollabuk-do, Korea. The diploid number of chromosomes was 42, and its karyotype was composed of 28 metacentrics and 14 submetacentrics; 84 FN (fundamental number). Polyploidy and sex dimorphism were not observed in the present species. The chromosome number of L. somjinensis was the same as its congeners, but there was a difference in karyotypes.

Karyotype Analysis of an Endemic Korean Torrent Catfish Liobagrus hyeongsanensis(Siluriformes: Amblycipitidae) (한국 퉁가리속 고유종 동방자가사리 Liobagrus hyeongsanensis의 핵형 분석)

  • Cho, Yun Jeong;Park, Jong Young
    • Korean Journal of Ichthyology
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    • v.29 no.2
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    • pp.89-93
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    • 2017
  • For the karyotype analysis of Liobagrus hyeongsanensis, an endemic Korean torrent catfish, ten females and five males were used from Yangbuk-myeon, Gyeongju-si, Gyeongsangbukdo, Korea. The diploid number of chromosomes was 42, and its karyotype consisted of 30 metacentrics and 12 submetacentrics; 84 FN (fundamental number), with having no polyploidy and sexual dimorphism. The chromosome number was the same as other closely-related species, L. mediadiposalis and L. somjinensis, but their karyotypes showed a clear difference by species. Such result may be related to chromosomal rearrangements by Robertsonian rearrangement with geographical isolation.

A comparative Karyotype study on Korean Squirrels. II. Karyotype Analysis of Sciurus vulgaris coreae and Tamias sibiricus asiaticus by G-banding Method. (한국산 다람쥐 핵형의 비교연구 II. G-banding 방법에 의한 한국산 청서(Sciurus vulgaris coreae)와 다람쥐(Tamias sibiricus asiaticus)의 핵형분석)

  • 김종봉;이희영
    • The Korean Journal of Zoology
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    • v.33 no.3
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    • pp.255-259
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    • 1990
  • The Karyotypes of Korean Sciurus uulgaris coreae and Tamias sibiricus asiaticus were analyzed by the G-banding method. Chromosomes of two species could be identified by G-banding patterns. The banding patterns of chromosomes 9, 10, 12 and X of S. vulgaris coreae were identical to those of chromosomes 6, 9, 12 and X, respectively of T. sibiricus asiaticus. It was shown that chromosomes 4, 10, 7 and 17 of T. sibiricus asiaticus resulted from pericentric inversion of chromosomes 1, 7, 8 and 16 of S. vulgaris coreae. These results suggested that pericentric inversion was an important factor in the karyological differentiation of two species.

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