• 생약학회지
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• 제30권3호
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• pp.244-249
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• 1999

In order to find less toxic antiviral agents from Basidiomycetes, EA, the water soluble substance, was prepared from the carpophores of Elfvingia applanata(Pers.) Karst. Antiviral activity of EA against vesicular stomatitis virus [Indiana serotype, VSV(IND)] was examined in Vero cells using plaque reduction assay in vitro. And the combined antiviral effects of EA with interferon (IFN) alpha or gamma were examined on the multiplication of VSV(IND). EA caused a concentration-dependent reduction in the plaque formation of VSV(IND) with 50% effective concentration $(EC_{50})$ of $104.02\;{\mu}g/ml$. The results of combination assay were evaluated by the combination index (CI) that was analysed by the multiple drug effect analysis. All cases of the combination of EA with IFN alpha or IFN gamma showed potent synergism with CI values of $0.38{\sim}0.52$ for $50{\sim}90%$ effective levels.

• Geomechanics and Engineering
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• 제25권1호
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• pp.59-73
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• 2021

There frequently exists inadequacy regarding the number of boreholes installed along tunnel alignment. While geophysical imaging techniques are available for pre-tunnelling geological characterization, they aim to detect specific object (e.g., water body and karst cave). There remains great motivation for the industry to develop a real-time identification technology relating complex geological conditions with the existing tunnelling parameters. This study explores the potential for the use of machine learning-based data driven approaches to identify the change in geology during tunnel excavation. Further, the feasibility for machine learning-based anomaly detection approaches to detect the development of clayey clogging is also assessed. The results of an application of the machine learning-based approaches to Xi'an Metro line 4 are presented in this paper where two tunnels buried in the water-rich sandy soils at depths of 12-14 m are excavated using a 6.288 m diameter EPB shield machine. A reasonable agreement with the measurements verifies their applicability towards widening the application horizon of machine learning-based approaches.

• Geomechanics and Engineering
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• 제38권1호
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• pp.69-77
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• 2024

The development of shield-driven cross-river tunnels in China is witnessing a notable shift towards larger diameters, longer distances, and higher water pressures due to the more complex excavation environment. Complex geological formations, such as fault and karst cavities, pose significant construction risks. Real-time adjustment of shield tunneling parameters based on parameter prediction is the key to ensuring the safety and efficiency of shield tunneling. In this study, prediction models for the torque and thrust of the cutter plate of ultra-large diameter slurry shield TBMs is established based on integrated learning algorithms, by analyzing the real data of Heyan Road cross-river tunnel. The influence of geological complexities at the excavation face, substantial burial depth, and high water level on the slurry shield tunneling parameters are considered in the models. The results reveal that the predictive models established by applying Random Forest and AdaBoost algorithms exhibit strong agreement with actual data, which indicates that the good adaptability and predictive accuracy of these two models. The models proposed in this study can be applied in the real-time prediction and adaptive adjustment of the tunneling parameters for shield tunneling under complex geological conditions.

• 지구물리와물리탐사
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• 제12권2호
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• pp.215-220
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• 2009

시멘트 모르타르는 고속도로 건설 및 댐 사이트의 연약지반 보강과 석회암 공동의 지반보강에 널리 사용되고 있다. 모르타르의 물리적 성질은 많은 연구 결과로부터 잘 알려져 있지만, 연약지반의 개량 정도를 평가하기 위해서 알아야 할 가장 중요한 물성 중의 하나인 전기비저항에 대해서는 극히 일부의 연구만 수행되어 있다. 이 연구에서는 물과 포틀랜드 시멘트의 배합비(0.35, 0.45, 0.50, 0.65)를 달리하여 원통형의 모르타르 시료를 제작하여, 웨너 배열 방식으로 전기비저항을 측정하였다. 그 결과 모르타르 시료의 전기비저항은 경과시간에 따라 증가하였으며, 함수비와 물/시멘트의 배합비가 증가할수록 감소하였다. 이 결과로부터 모르타르의 전기비저항과 압축강도는 밀접한 관계를 가지고 있음을 알 수 있었다.

• 동굴
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• 제66호
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• pp.45-57
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• 2005

This Paper is a study on the duality of speleothem that appeared in 'Hyeob Jae Cave' which is designated as the natural monumen. No.236. It is located at Hyeob Jae Ri, Hanrim Eub, Bug Je-ju Gun, Je-judo. The findings are as follows. 1. The distribution range of the shelly sand which has maximum thickness of $10m{\pm}$ and average of $3m{\pm}$ was $3.2m^2$. 2. The desert hollow acted to promote the speleothem deposits in the lava tunnel with lava mound formed by lavapilz and artificial breaksand wall. 3. The main component of the pseudo limestone cavern was carbonate Calcium from shelly sand. And the deposition of speleothem in the Cave was accelerated after the volcanic erupsion of Biyang island in 1002. A.D. 4. The secondary depositions of Calcite recognized as speleothem up to now it can be used for the pseudo karst in general. 5. It seems that the variety of the cave deposits is decided depending upon the geology, land form, climate, vegetations and the structural environmental factors. 6. It seems that the wondering development of accretionary deposits caused by encrusting has a close relation with intermittent seepage of ground water. 7. Finally, we can acknowledge the coexistent duality of speleothem by shelly sand along with the joint and the lava stalactites formed at the same time with the lava tunnel on the ceiling where there was no seepage.

• 물과 미래
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• 제16권4호
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• pp.245-252
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• 1983

근년에 이르러 국내 지표수의 오염 현상은 사회적으로 매우 심각한 문제를 야기시키고 있어 수자원의 보호문제가 매우 중요한 시점에 이르렀다. 한강유역내에 발달 분포된 천층의 충적 퇴적층과 결정질암내에 저유된 지하수에 대해 동위원소를 이용한 환경 조사를 시행하였으며 결과를 분석한 바 다음과 같은 가정을 확인하였다. 1) 천층지하수의 함양원은 서울 도심지역은 하천수였고, 교외 외각의 농촌 지역은 강우의 직접 지하 침투에 의해 일어나고 있으며, 2) 충적 퇴적층으로 피복된 결정질 암은 상부충적퇴적층내에 저유된 천층 지하수가 충진원의 역할을 한다. 한강하류부의 해안 가까운 곳에 분포된 일부 충적층과 그 하부의 결정질암내에 저유된 지하수는 $H^3$의 함량이 매우 낮은치를 나타내는데 이는 핵실험이전의 기상수가 상당히 먼곳에서 충진되었기 때문으로 시료된다. 안정동위원소 조사에 의하면 한강상류에 분포된 석회암 대수층의 함양지역은 하류부의 충적층과는 달리 비교적 고지대였으며 석회암 대수층내에서 지하수의 체제 시간은 수개월 정도였다. 지하수의 포함된 극소량의 Pb, Mn, Zn 량과 충진메카니즘을 대비하려고 시도하였으나 명확한 관계가 나타나지 않았다.

• 한국균학회지
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• 제27권2호통권89호
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• pp.175-179
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• 1999

잔나비걸상 Elfvingia applananta 자실체의 수용성물질 EA의 vesicular stomatitis virus[New Jersey serotype, VSV(NJ)]에 대한 항바이러스효과를 plaque reduction assay에 따라 실험한 결과 EA는 용량의존적으로 plaque 형성을 억제하였으며 $EC_{50}$는 2.10 mg/ml이었다. 단백질성 항바이러스제인 interferon(IFN)과 EA와의 병용시험 결과 f(a)가 0.50에서 0.90인 유효농도범위에서 IFN alpha와 병용시 f(a)의 값이 커짐에 따라 상가작용 내지는 길항작용을 나타내었으며, IFN gamma와의 병용시에는 길항작용을 나타내었다. 따라서 IFN alpha와의 병용시 f(a)가 0.50내지 0.70의 유효농도 범위에서 상가효과가 있다.

• Archives of Pharmacal Research
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• 제24권1호
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• pp.74-78
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• 2001

A preparation of water soluble components (EA) was made from carpophores of Elfvingia applanata (Pers.) Karst and its in vitro antiviral activity on vesicular stomatitis virus [(Indiana serotype, VSV(IND)] was investigated by plaque reduction assay. EA exhibited potent antiviral activity on VSV(IND) growth and negligible cytotoxicity on Vero cells, 50% effective concentration ($EC_{50}C$/) of 104$ug\textrm\/ml$ and 50% cytotoxic concentration ($CC_{50}C$) of 3,793$ug\textrm\/ml$, respectively. Selectivity index (Sl $CC_{50}C$/$EC_{50}C$) of EA on Vero cell and VSV(IND) was about 36.5. EA did not display either a direct virucidal effect on V5V(IND) or induction of antiviral substance by Vero cells upon its treatment. Thus, the mode of antiviral activity of EA was studied at steps of viral adsorption onto cell. When both EA and virus were added to cell monolayers, titer of cell-free virus in culture supernatant increased in ca. 30-40% compared with that of control group and titer of cell-associated virus was 60-100% higher than that of control group. These results suggested that antiviral activity of EA on VSV(IND) might be due to the hindrance of viral entry to cells at eITher endocytosis or loss of envelope.

• 한국식품조리과학회지
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• 제21권6호통권90호
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• pp.759-768
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• 2005

This study was performed to determine the antioxidative effect of the hexane, dichloromethane, ethylacetate, butanol and water fractions of Ganoderma lucidum extracts on the inhibition of malondialdehyde(MDA) and bovine serum albumin(BSA) conjugation reaction, the inhibition of lipid peroxidation and the scavenging effect on 1,1-diphenyl-2-picryl-hydrazyl(DPPH) radical, the antimutagenic capacity as measured by the Ames test and the inhibitory effect on cancer cell. Ganoderma lucidum is believed to have possible antioxidative capacities, although the results have varied according to the assay method. The most effective antioxidative capacity was inhibition of lipid peroxidation. Among the five fractions, water fraction showed strong inhibition rates on MDA & BSA conjugation reaction, and ethylacetate fractions showed the most effective inhibition rate on lipid peroxidation and scavenging effect on DPPH radical. The indirect and direct antimutagenic effects of ethanol extracts of Ganoderma lucidum were examined by Ames test using Salmonella typhimurium TA98 and TA100. Among the samples, the water fraction did not have any antimutagenic effect. The inhibition rates on mutagenicity in the presence of 2.5 mg/plate were nearly $100\%$ for Salmonella typhimurium TA98 and TA100 except the hexane fraction of the direct mutagenicity mediated by 2-Nitrofluorene in Salmonella typimurium TA98($64.69\%$). Under the 2.5 mg/plate concentration, the inhibitory effects of hexane and dichloromethane fraction were superior to that of the other fractions on the direct mutagenicity for Salmonella typhimurium TA100 and indirect mutagenicity for Salmonella typhimurium TA98 and TA100. The inhibitory effect of Ganoderma lucidum extracts on cell proliferation in HeLa and MCF-7 was investigated by U test. The dichloromethane fraction showed highly antiproliferative effect in HeLa and MCF-7($IC_{50}$: 0.122 mg/mL, 0.272 mg/mL, respectively) cells while the water faction had a weak inhibitory effect($IC_{50}$: 0.691 mg/mL, 10.919 mg/mL respectively). These results suggest that Ganoderma lucidum may have antioxidative, antimutagenic and anticancer capacities and may be a candidate of the prevention and dietetic treatment of chronic diseases and the development of antioxidative, antimutagenic and anticancer functional food.

• 터널과지하공간
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• 제31권4호
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• pp.211-220
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• 2021

한국의 대심도 (>40m 깊이) 터널 공사 시에 터널 붕괴 사고가 종종 일어나고 있으며, 도심지 지하공간의 얕은 심도에 인공적으로 조성된 지반에는 자연 공동뿐만 아니라 상수도관, 하수도관, 전력구 및 지하철 건설로 인한 인위적인 공동들이 복잡하게 분포되어 있다. 대심도 터널 굴착을 위해서는 이러한 다공질의 특성을 보이는 다양한 지반의 특성 및 지질구조가 지반의 안전에 미치는 영향을 이해하여야 한다. 본 연구는 국내외 사례를 바탕으로 한국의 대심도 굴착에서 암반의 위험 산정을 위한 위험 인자를 분석하였다. 연구결과, 대심도 터널 굴착시 지반의 안정성에 영향을 주는 총 7개의 카테고리들과 총 38개의 인자들이 도출되었다. 가중치가 상대적으로 높은 인자들은 단층 및 단층점토, 차응력, 암종, 지하수 및 머드 유입, 암석의 일축압축강도, 터널 단면의 크기, 터널 상부 암반의 두께, 카르스트 및 계곡지형, 습곡, 석회암의 협재, 지하수위 변동, 터널 심도, 암맥, RQD, 절리 특성, 이방성, 암반파열(rockburst) 등으로 나타났다.