• The Journal of Korean Medicine
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• v.40 no.3
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• pp.35-58
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• 2019

Objectives: The aim of this study was to investigate the anti-inflammatory effects of Curcuma longa rhizoma extract in an experimental rat model of osteoarthritis. Methods: Osteoarthritis was induced in rats by injecting monosodium iodoacetate (MIA) into the knee joint cavity of rats. The rats were divided into 5 groups (Normal, Control, positive comparison, low (CL) and high (CH) concentration groups). Rats in the low concentration (CL) group had MIA-induced osteoarthritis; they were treated with Curcuma longa rhizoma extract at a dose of 50mg/kg body weight. Rats in the high concentration (CH) group had MIA-induced osteoarthritis; they were treated with Curcuma longa rhizoma extract at a dose of 100mg/kg body weight. Hind paw weight distribution and ROS levels were measured. At the end of all treatments, changes in alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), and creatinine levels were analyzed. In addition, inflammatory protein levels were evaluated by western blot analysis. Results: In this study, hind paw weight distribution significantly improved in the CL and CH groups, while. Reactive oxygen species (ROS) production significantly decreased in both. The levels of ALT, AST, BUN, and creatinine did not significantly change in either group. The production of nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4), $p47^{phox}$, and Ras-related C3 botulinum toxin substrate 1 (RAC1) decreased in both. Catalase, heme oxygenase-1 (HO-1) and superoxide dismutase (SOD) significantly increased in the CL and CH groups, respectively. Nuclear factor erythroid 2 (Nrf2) increased, but there were no significant differences between the experimental and control groups. Inflammatory cytokines, including nuclear factor-kappa Bp65 (NF-${\kappa}Bp65$), interleukin-1beta (IL-$1{\beta}$), and tumor necrosis factor-alpha (TNF-${\alpha}$), decreased significantly in both the CL and CH groups. Conclusions: Our results showed that Curcuma longa rhizoma extract has anti-inflammatory effects. Anti-inflammatory activity is regulated by the inhibition of inflammatory cytokines and mediators, such as NF-${\kappa}B$, therefore, it suppresses cartilage damage as well.

• The Journal of Internal Korean Medicine
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• v.31 no.3
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• pp.513-525
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• 2010

Objectives : This study was carried out to investigate the effects of Goihwa-san(GHS) against ulcerative colitis induced by DSS (dextran sulfate sodium). Method : The sample group was divided into three. The control group consisted of mice that were not inflammation-induced. The pathological group was composed of untreated colitis elicited mice. The experimental group was administered GHS after colitis elicitation. The effects on ulcerative colitis were evaluated by the morphological change of colonic mucosa, the anti-oxidant effect, HSP 70, NF-${\kappa}$B, COX-1, COX-2 and iNOS. Results : In terms of immunohistochemical changes, the distribution of COX-1 in mice treated with GHS increased noticeably more than that in the pathological group. The distributions of HSP70, NF-${\kappa}$B, COX-2, iNOS in mice treated with GHS more decreased than those in the pathological group. Regeneration of surface epithelial cell and goblet cell in mucosa was observed by optical microscope. The colonic lengths in GHS-treated mice were more elongated than those of DSS only treated mice. Conclusion : GHS is a candidate treatment for ulcerative colitis.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.23
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• pp.10381-10385
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• 2015

SKI-II has been reported as an inhibitor of sphingosine kinase 1 and has been extensively used to prove the involvement of sphingosine kinase and sphingosine-1-phosphate (Sphk1) in cellular processes. In the current study, we investigated the effects of SKI-II and its potential mechanisms in human gastric cancer SGC7901 cells. After treatment with SKI-II, cell growth, cell cycle distribution, apoptosis, expression of Sphk1, NF-${\kappa}B$, Bcl-2, Bax and p27 were assessed by MTT assay, flow cytometry, electron microscopy, immunocytochemistry and Western-blot assay, respectively. Our results showed that SKI-II markedly inhibited SGC7901 cell survival in a dose-dependent manner, reduced cell proliferation with accumulation of cells in the G0/G1 phase and induced apoptosis in the tumor cells. Furthermore, Western blotting and immunocytochemistry showed that the expression of p27 and Bax was increased significantly, but the expression of NF-${\kappa}B$, Bcl-2 and Sphk1 decreased by different degrees. These results indicate that SKI-II induced cell growth arrest and apoptosis. The increased apoptotic sensitivity of SGC7901 was correlated with NF-${\kappa}B$ or Bcl-2/Bax activation.

• Journal of Sasang Constitutional Medicine
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• v.11 no.1
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• pp.159-168
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• 1999

Purpose : Sasang Constitutional Medicine explains the pathology peculiar to constitution and suggests treatment for each constitution. In Sasang Constitutional Medicine hwnan beings are classified on four groups; Taeyangin, Taeumin, 5oyangin, Soeumin. These four constitution has their's own symptoms and treatments. In treatment, control of mind inclination, that is to say, moderation takes a very important role. But the study on heredity in Sasang Constitutional Medicine has not done not biological study but also statistical study. So we used several statistical methods and analyzed 163 samples. Methods : We implemented Fishers exact test for adjusting chi-squared test, kappa coefficient to estimate agreement of parent's and children's constitutions, and finally plotted bi-plot using correspondence analysis. Results : From Fisher's exact test result, we could know that parent's and children'S constitution's distribution had significant difference. In kappa coefficient, mother and daughter's estimated value produced highest result. In correspondence analysis we only plotted the case of mother and son for easy interpretation. Conclusion : In the study of heredity of SaSang constitution, we cannot know exactly the heredity of constitution in terms of biology or genetics. But this research can be helpful for further analysis, that is, a study of biological or genetical aspects. And we could conclude that in statistical aspects the heredity in SaSang constitution is meaningful.

• 한국전산유체공학회:학술대회논문집
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• 2007.10a
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• pp.187-193
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• 2007

The flight vehicles have cavities such as wheel wells and bomb bays. The flow around a cavity is characterized as unsteady flow because of the formation and dissipation of vortices due to the interaction between the freestream shear layer and cavity internal flow, the generation of shock and expansion waves. Resonance phenomena can damage the structures around the cavity and negatively affect aerodynamic performance and stability. In the present study, numerical analysis was performed for cavity flows by the unsteady compressible three dimensional Reynolds-Averaged Navier-Stokes (RANS) equations with Wilcox's ${\kappa}\;-\;{\omega}$ turbulence model. The cavity has the aspect ratios of 2.5, 3.5 and 4.5 for two-dimensional case, same aspect ratios with the W/D ratio of 2 for three-dimensional case. The Mach and Reynolds numbers are 0.53 and 1,600,000 respectively. The flow field is observed to oscillate in the "shear layer mode" with a feedback mechanism. Based on the SPL(Sound Pressure Level) analysis of the pressure variation at the cavity trailing edge, the dominant frequency was analyzed and compared with the results of Rossiter's formula. The MPI(Message Passing Interface) parallelized code was used for calculations by PC-cluster.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.20 no.5
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• pp.1661-1670
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• 1996

Incompressible flow over a backward-facing step is computed by low Reynolds number turbulence models in order to compare with direct simulation results. In this study, selected low Reynolds number 1st and 2nd (Algebraic Stress Model : ASM) moment closure turbulence models are adopted and compared with each other. Each turbulence model predicts different flow characteristics, different re-attachment point, velocity profiles and Reynolds stress distribution etc. Results by .kappa.-.epsilon. turbulence models indicate that predicted re-attachment lengths are shorter than those by standard model. Turbulent intensity and eddy viscosity by low Reynolds number .kappa.-.epsilon. models are still greater than DNS results. The results by algebraic stress model (ASM) are more reasonable than those by .kappa.-.epsilon. models. The convective scheme is QUICK (Quadratic Upstream Interpolation for Convective Kinematics) and SIMPLE algorithm is adopted. Reynolds number based on step height and inlet free stream velocity is 5100.

• The Journal of Internal Korean Medicine
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• v.25 no.3
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• pp.497-507
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• 2004

This study was carried out to investigate the effects of Ikwitang extract on indomethacin-induced gastric mucosal lesions in mice. Experimental groups were classified into a non-treated group(NM group), a non-administered group(CON group), the Misoprostol-administered group(MI group) and the Ikwitang-extract-administered group(IW group). This study examined the morphological change, distribution of mast cells, mucous surface cells, acid mucose secreted cells, and apoptic cells, BrdU, COX-1, Hsp70, $NF-{\kappa}B\;p50$, PKC, COX-2 and IL-12B of gastric mucosa. Results : The results of this study were as follows: 1. In the Misoprostol-administered group and the Ikwitang-extract-administered group, the hemorrhagic erosion of gastric mucous and infiltrated mast cells decreased. 2. Mucous surface cells and acid mucose secreted cells abserved in the Misoprostol-administered group and the Ikwitang-extract administered group. 3. The distribution of apoptic cells, Hsp70, $NF-{\kappa}B\;p50$, PKC, COX-2, and IL-12B increased in the Control group, but decreased in the Misoprostol-administered group and Ikwitang-extract-administered group. 4. Cells proliferation of gastric mucosa and the COX-l positive cells decreased in the Control group, but increased in the Misoprostol-administered group and the Ikwitang-extract -administered group. The above results suggest the lkwitang extract had beneficial effects on indomethacin induced gastric mucosal lesions.

• Korean Journal of Agricultural Science
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• v.20 no.1
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• pp.56-67
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• 1993

To applying of genetic markers of milk proteins as dairy cow registration and selection aids for genetic improvement, genopypes controlling the 4 milk protein loci, ${\alpha}S1$-casein (${\alpha}S1$-CN), ${\beta}$-casein(${\beta}$-CN), ${\kappa}$-casein(${\kappa}$-CN), and ${\beta}$-lactoglobulin(${\beta}$-LG), from a total of 159 Holstein lactating cows reared at National Animal Breeding Station in 1992 were detected by polyacrylamide gel(PAGE) electrophoresis, and associations between genetic polymorphisms of milk proteins and milk compositions were analyzed. The observed distribution of phenotypes for ${\alpha}S1$-CN, ${\beta}$-CN, ${\kappa}$-CN and ${\beta}$-LG were agreement with those expected under the assumption of genetic equilibrium. The observed genotypic frequencies of the ${\alpha}S1$-CN BB, ${\beta}$-CN AA, ${\kappa}$-CN AA and ${\beta}$-LG AB genotypes were founded to be very high as 79.87%, 84.28%, 71.70% and 49.10%, respectively. Gene frequencies were 0.899 and 0.101 for ${\alpha}S1-CN^B$ and ${\alpha}S1-CN^C$, 0.921 and 0.079 for ${\beta}-CN^A$ and ${\beta}-CN^B$, 0.837 and 0.163 for ${\kappa}-CN^A$ and ${\kappa}-CN^B$, 0.378 and 0.622 for ${\beta}-LG^A$ and ${\beta}-CN^B$. According to the results of analysis of variance, the genotypes of the ${\alpha}S1-CN$, ${\beta}-CN$, ${\kappa}-CN$ and ${\beta}-LG$ were significantly difference for fat, protein and total solid percentage in milk compositions. On milk compositions, the ${\kappa}$-CN BB genotype was very high fat and protein percentage more than ${\kappa}$-CN AA and AB genotypes, and ${\beta}$-LG AA genotype was very high fat percentage more than ${\beta}$-LG AB and BB genotype at 5% level of significant difference, respectively. As a consequence, the fat and protein percentage may be improved to select to ${\kappa}$-CN BB and ${\beta}$-LG AA genotypes.

• Journal of Korea Water Resources Association
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• v.30 no.1
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• pp.3-13
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• 1997

Modifications of Prandtl's mixing length theory were used to obtain a power velocity distribution in which the coefficient and exponent are variable over a range from 1/4 to 1/7. A simple suspended-sediment concentration distribution was developed which can be associated with this modified velocity distribution. Using nominal values of ${\beta}$=1.0, $\kappa$=0.4 and visual accumulation tube values of fall velocity, the comparison between theory and field measurements by the USGS on the Rio Grande is fair. Doubling the value of the exponent results in a good comparison. Further research is needed to be able to better choose ${\beta}$, $\kappa$, and fall velocity values, but such research will not be able to account for the effects of large-scale turbulence and secondary flows. In a pragmatic sense, a special set of fairly detailed measurements can establish coefficients and exponents for any gaging site.

• 한국전산유체공학회:학술대회논문집
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• 2001.10a
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• pp.44-50
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• 2001

A numerical study was made to choose the better turbulence model for the flow in the discharge flow path from a diffuser to a wall. In this study standard $\kappa-\epsilon$ model(SKE), RNG $\kappa-\epsilon$ model(RNG), and Reynolds stress model(RSM) were applied. In case of the flow with relatively high Reynolds number at a diffuser inlet, the pressure loss coefficients by RNG have a tendency to be near to those by SKE at small ratio(below about 0.35) of $h/D_o$, but to those by RSM at large ratio(above about 0.35). At large ratio RNG begins to enlarge the effects of rapid strain and streamline curvature. RNG & RSM are recommended as the appropriate turbulence models for this case. But it is noticeable that the velocity gradient pattern in RNG is same as in SKE, and also that the total pressure distribution in RNG is same as in RSM only at swirling flow area, same as in SKE only at main flow area.