• The Plant Pathology Journal
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• v.21 no.3
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• pp.262-269
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• 2005

An antiviral producing bacterial strain was isolated from a ginseng rhizosphere in Kangwon province of Republic of Korea. In order to identify the bacterial strain, microbiological, physiological and biochemical tests were performed, along with RAPD, 16S rRNA, 16S-23S rRNA ITS (intergenic spacer region) and DNA-DNA hybridization analyses. The bacterium was found to be a strain of Pseudomonas fluorescens, which was designated as Gpf01. The strain was grown in Muller-Hinton (MH) broth, and the culture supernatant obtained was filtered through a $0.45{\mu}l$ filter. It was further boiled at $100^{\circ}C$ and tested in two experiments for its ability to control a yellow strain of Cucumber mosaic virus (CMV-Y). In the first experiment, boiled culture filtrate (RCF) was treated on one half of the leaves of Chenopodium amaranticolor followed by CMV- Y inoculation on both halves. In the second experiment, BCF was treated on the lower leaves of Nicotiana tobacum var. Xanthi-nc, with the CMV-Y mechanically inoculated onto the upper untreated leaves. In the first experiment, BCF treatment was able to considerably reduce the number of viral lesion, and in the second experiment, plants treated with BCF showed no visible viral symptoms compared to the Muller-Hinton (MH) media treated controls 15 days post inoculation (dpi), and remained symptomless throughout the study period. Thus, Gpf01, identified as P. fluorescence, was able to produce an antiviral component in the culture filtrate, which was found to be heat stable, non-phytotoxic and effective in local as well as systemic hosts of CMV.

• Journal of Korean Society on Water Environment
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• v.31 no.5
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• pp.543-555
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• 2015

The existing standard for soil erosion risk assessment has limitations in sustainable topsoil management since the fixed criteria are applied to determination of soil erosion risk areas regardless of land use types. It may not be necessary to apply soil erosion best management practices to agricultural areas with high potential of soil erosion because human or economic damage derived from soil erosion might be tiny in that region. Furthermore, the fixed criterion with absolute values can select too many hot spots of soil erosion to conduct efficient soil erosion management. Thus, objective of this study was to suggest the relative criteria using statistical analysis for efficient soil erosion management. In future, the relative indices for soil erosion prevention should be improved to provide a priority of soil erosion management considering economic damage from soil erosion or functional values of soil with quantitative soil erosion. Additional researches will be needed to reflect a regional characteristics and to consider various land use types and different criteria.

• Journal of The Korean Society of Agricultural Engineers
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• v.66 no.2
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• pp.25-34
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• 2024

The long-term monitoring of the Soyang Lake's water quality, covering 25% of the North Han River watershed, is crucial for effective management of both lake water quality and pollution sources in the broader region. This study utilized continuous monitoring data from the front of the Soyang Dam spanning 2003 to 2022, aiming to analyze trends and provide foundational insights for water quality management. Results revealed a slightly poor grade (IV) for total nitrogen (T-N) in both surface and mid-depth layers, indicating a need for concentrated T-N management. Trend analyses using the Mann-Kendall test and Sen's Slope depicted a decreasing trend in total phosphorus (T-P) for both layers, attributed to non-point source pollution reduction projects initiated after the Soyang Lake's designation as a pollution control area in 2007. The LOWESS analysis showed a T-P increase until 2006, followed by a decrease, influenced by the impact of Typhoon Ewiniar in that year. This 20-year overview establishes a comprehensive understanding of the Soyang Lake's water quality and trends, allowing for a seasonal and periodical analysis of water quality changes. The findings underscore the importance of continued monitoring and management strategies to address evolving water quality issues in the Soyang Lake over time.

• Journal of Microbiology
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• v.39 no.1
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• pp.31-36
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• 2001

A cDNA clone encoding the ribosomal protein S20 has been isolated from the Schizosaccharomyces pombe cDNA library by colony hybridization. The insert contained in the original plasmid pYJ10 was transferred intro shuttle vector pRS316 generate plasmid pYJll. The dDNA insert of plasmid pYJll, contains 484 nucleotides and encodes a protein of 118 amino acids with a calculated mass of 13,544 daltons. The deduced amino acid sequence of S. pombe ribosomal protein S20 is very homologous with fruit fly, rat, and budding yeast counterparts. It is also homologous with Xenopus S22 ribosomal protein. S. pombe ribosomal protein S20 appears to be relatively hydruphobic except the C-terminal region. The 728 bp upstream region of the S20 gene was amplified from chromosomal DNA and transferred into the BamHI/EcoRI site of the promoterles $\beta$-galactosidase gene of the vector YEp357R, which resulted in fusion plasmid pYS20. The synthesis of $\beta$-galactosidase from the fusion plasmid appeared to be the highest in the mid-exponential phase. The S. pombe cells with the fusion plasmid grown at 35$\^{C}$ gave lower $\beta$-galactosidase activity than the cells grown at 30$\^{C}$. Computer analysis showed the consensus sequence CAGTCACA in the upstream regions of various ribosomal protein genes in S. pombe, which would be involved in the coordinated expression of small ribosomal proteins.

• Molecules and Cells
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• v.20 no.1
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• pp.74-82
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• 2005

Glutaredoxins (Grxs) are thioloxidoreductases which are required for maintaining thiol/disulfide equilibrium in living cells. The Grx3 gene, which encodes one of the three monothiol Grxs in the fission yeast Schizosaccharomyces pombe, was characterized, and its transcriptional regulation studied. Genomic DNA encoding Grx3 was isolated by PCR, and a plasmid pTT3 carrying this DNA was produced. The DNA sequence has 1,267 bp, which would encode a monothiol Grx of 166 amino acids with a molecular mass of 18.3 kDa. The putative protein has 27% homology with Grx5, and contains many hydrophobic amino acid residues in its N-terminal region. S. pombe cells harboring pTT3 had increased Grx activity and enhanced survival on minimal medium plates containing aluminum (5 mM), BSO (0.05 mM), menadione (0.01 mM) or cadmium (0.2 mM). The 568 bp upstream region of Grx3 was fused into the promoterless b-galactosidase gene of the shuttle vector YEp367R to generate fusion plasmid pMJS10. Potassium chloride (KCl) and metals including aluminum and cadmium enhanced the synthesis of ${\beta}$-galactosidase from the fusion gene. The synthesis of ${\beta}$-galactosidase was also enhanced, in a Pap1-dependent manner, by fermentable carbon sources such as glucose (at low concentrations) and sucrose, but not by non-fermentable carbon sources such as ethanol and acetate. Grx3 mRNA increased in response to treatment with BSO. These observations indicate that S. pombe Grx3 is involved in the response to stress, and is regulated by stress.

• Korean Journal of Medicinal Crop Science
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• v.28 no.5
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• pp.325-330
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• 2020

Background: Dried tuberous roots of Cynanchum wilfordii are known to relieve menopause symptoms. However, the dried roots of C. wilfordii are morphologically similar to those of C. auriculatum, which makes it difficult to distinguish when used as a medicine. Various comparative studies have focused on chemical or molecular analysis of these roots. However, the differences between the two species at the cytogenetic level based on chromosome structure and composition remain to be elucidated. Methods and Results: For chromosome slides, the roots were fixed in 8-hydroxyquinoline, digested with enzyme mixture, and spread on slides. 5S and 45S rDNA were used as cytogenetic markers for the analysis of nuclear genomes by FISH. The chromosome number of the two species was 2n = 22, with a relatively short length, 1.13 ㎛ - 4.24 ㎛ and 1.00 ㎛ - 3.42 ㎛ with respect to each other. Both species represent one pair of 5S and 45S rDNA signal on chromosome 1, at the proximal region and peri-centromeric region, respectively. Conclusions: These preliminary cytogenetic data using FISH in C. wilfordii and C. auriculatum could be valuable for the comprehension of Cynanchum genome history.

• Journal of Forest and Environmental Science
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• v.11 no.1
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• pp.61-71
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• 1995

From Salix maximowiczii of the Korean Salix, morphological and palynological characters were investigated by stereoscopic, light, and scanning electron microscopes. Also, this study was eonducted to investigate the effect of meteorological factors on the disjunct distribution in Salix maximowiczii forests grown within the restricted region. 1. Morphological characters of S. maximowiczii clarified with the descriptions and figures. 2. The palynological description was made to the S. maximowiczii and identified distinctly species. 3. S. maximowiczii is distributed only in Baekdam and Hangyeryong valley of Mt. Sorak in Kangwon-do, and valley of height region in Kyeongseong-gun (Hamgyongbuk-do), Sinhueng-gun, and Pungsan-gun (Hamgyongnam-do) in North Korea. 4. Water factors was better than temperature factors in the influence of meteorological factors for disjunct distribution. 5. These ecological and taxonomical informations could be used in practical application for silvicultural prescription, such as erosion control forest, timber production, and development of green area in river bank.

• Korean Journal of Ecology and Environment
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• v.41 no.2
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• pp.247-263
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• 2008

A two-dimensional (2D) reservoir hydrodynamics and water quality model, CE-QUAL-W2, is employed to simulate the hydrothermal behavior and density current regime in Andong Reservoir. Observed data used for model forcing and calibration includes: surface water level, water temperature, dissolved oxygen and suspended solids concentration. The model was calibrated to the year of 2003 and verified with continuous run from 2000 till 2004. Without major adjustments, the model accurately simulated surface water levels including the events of large storm. Deep-water reservoirs, like Andong Reservoir, located in the Asian Monsoon region begin to stratify in summer and overturn in fall. This mixing pattern as well as the descending thermocline, onset and duration of stratification and timing of turnover phenomenon were well reproduced by the Andong Model. The temperature field and distinct thermocline are simulated to within $2^{\circ}C$ of observed data. The model performed well in simulating not only the dissolved oxygen profiles but also the metalimnetic dissolved minima phenomenon, a common1y occurring phenomenon in deep reservoirs of temperate regions. The Root Mean Square Error (RMSE) values of model calibration for surface water elevation, temperature and dissolved oxygen were 0.0095 m, $1.82^{\circ}C$, and $1.13\;mg\;L^{-1}$, respectively. The turbid storm runoff, during the summer monsoon, formed an intermediate layer of about 15 m thickness, moved along the metalimnion until being finally discharged from the dam. This mode of transport of density current, a common characteristic of various other large reservoirs in the Asian summer monsoon region, was well tracked by the model.

• Journal of Forest and Environmental Science
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• v.33 no.4
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• pp.348-354
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• 2017

Agroforestry has been practiced in arid and semi-arid regions for the purposes of preventing desertification and to increase income for locals. However, the intended effects of such practices have been limited due to strong winds and aridity. This study undertook multi-year monitoring of the productivity of income crops associated with windbreak planting in a semi-arid region of Mongolia, and explored strategies of windbreak planning to enhance the multi-purpose effects of agroforestry practices. The tree crown density of windbreak planting was on average 40% in one year after planting and 65% 2-3 years after, and thereby windspeeds were reduced by about 30% and 54%, respectively. Average windspeed reductions at leeward distances from the windbreak planting were approximately 60% within 3H (H=tree height), 50% at 5H, and 42% at 7-9H, presenting a pattern in which the farther the distance the less the reduction in windspeeds. The windbreak planting increased crop productivity by up to 6.8 times, compared to the productivity absent of windbreaks. Increases in the crown density as stated above resulted in increases of crop productivity by up to 3.6 times. Based on such results, this study proposed a model of windbreak planning as a typical land-use system of border windbreak planting or alternate windbreak planting of combining trees and income crops. The model also included tree planting with a crown density of 60% and allocation of income crops within a leeward distance of 5 times the height of the trees to reduce windspeeds by about 50%. The results from this study are applicable to practicing agroforestry not only at the study site but also in other regions worldwide where strong winds and aridity are problematic.

• Mycobiology
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• v.41 no.2
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• pp.86-93
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• 2013

Sequence characterized amplified region (SCAR) markers are one of the most effective and accurate tools for microbial identification. In this study, we applied SCAR markers for the rapid and accurate detection of Phytophthora katsurae, the casual agent of chestnut ink disease in Korea. In this study, we developed seven SCAR markers specific to P. katsurae using random amplified polymorphic DNA (RAPD), and assessed the potential of the SCAR markers to serve as tools for identifying P. katsurae. Seven primer pairs (SOPC 1F/SOPC 1R, SOPC 1-1F/SOPC 1-1R, SOPC 3F/SOPC 3R, SOPC 4F/SOPC 4R, SOPC 4F/SOPC 4-1R, SOPD 9F/SOPD 9R, and SOPD 10F/SOPD 10R) from a sequence derived from RAPD fragments were designed for the analysis of the SCAR markers. To evaluate the specificity and sensitivity of the SCAR markers, the genomic DNA of P. katsurae was serially diluted 10-fold to final concentrations from 1 mg/mL to 1 pg/mL. The limit of detection using the SCAR markers ranged from $100{\mu}g/mL$ to 100 ng/mL. To identify the limit for detecting P. katsurae zoospores, each suspension of zoospores was serially diluted 10-fold to final concentrations from $10{\times}10^5$ to $10{\times}10^1$ zoospores/mL, and then extracted. The limit of detection by SCAR markers was approximately $10{\times}10^1$ zoospores/mL. PCR detection with SCAR markers was specific for P. katsurae, and did not produce any P. katsurae-specific PCR amplicons from 16 other Phytophthora species used as controls. This study shows that SCAR markers are a useful tool for the rapid and effective detection of P. katsurae.