Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Cytogenetical Study of Cynanchum wilfordii and Cynanchum auriculatum using Fluorescence In Situ Hybridization (FISH)

rDNA FISH를 이용한 큰조롱과 넓은잎큰조롱의 세포유전학적 연구

  • Kim, Su Hyun (Department of Applied Plant Science, Kangwon National University) ;
  • Kim, Woo Cheol (Department of Applied Plant Science, Kangwon National University) ;
  • Kim, Hyun Hee (Department of Life Science, Sahmyook University) ;
  • Heo, Kweon (Department of Applied Plant Science, Kangwon National University)
  • 김수현 (강원대학교 생물자원과학과) ;
  • 김우철 (강원대학교 생물자원과학과) ;
  • 김현희 (삼육대학교 생명과학과) ;
  • 허권 (강원대학교 생물자원과학과)
  • Received : 2020.07.09
  • Accepted : 2020.08.26
  • Published : 2020.10.30
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Abstract

Background: Dried tuberous roots of Cynanchum wilfordii are known to relieve menopause symptoms. However, the dried roots of C. wilfordii are morphologically similar to those of C. auriculatum, which makes it difficult to distinguish when used as a medicine. Various comparative studies have focused on chemical or molecular analysis of these roots. However, the differences between the two species at the cytogenetic level based on chromosome structure and composition remain to be elucidated. Methods and Results: For chromosome slides, the roots were fixed in 8-hydroxyquinoline, digested with enzyme mixture, and spread on slides. 5S and 45S rDNA were used as cytogenetic markers for the analysis of nuclear genomes by FISH. The chromosome number of the two species was 2n = 22, with a relatively short length, 1.13 ㎛ - 4.24 ㎛ and 1.00 ㎛ - 3.42 ㎛ with respect to each other. Both species represent one pair of 5S and 45S rDNA signal on chromosome 1, at the proximal region and peri-centromeric region, respectively. Conclusions: These preliminary cytogenetic data using FISH in C. wilfordii and C. auriculatum could be valuable for the comprehension of Cynanchum genome history.

Keywords

References

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