• The Korean Journal of Physiology and Pharmacology
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• v.20 no.3
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• pp.253-259
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• 2016

Previously, we found that KTH-13 isolated from the butanol fraction of Cordyceps bassiana (Cb-BF) displayed anti-cancer activity. To improve its antiproliferative activity and production yield, we employed a total synthetic approach and derivatized KTH-13 to obtain chemical analogs. In this study, one KTH-13 derivative, 4-(tert-butyl)-2,6-bis(1-phenylethyl)phenol (KTH-13-t-Bu), was selected to test its anti-cancer activity. KTH-13-t-Bu diminished the proliferation of C6 glioma, MDA-MB-231, LoVo, and HCT-15 cells. KTH-13-t-Bu induced morphological changes in C6 glioma cells in a dose-dependent manner. KTH-13-t-Bu also increased the level of early apoptotic cells stained with annexin V-FITC. Furthermore, KTH-13-t-Bu increased the levels of cleaved caspase-3 and -9. In contrast, KTH-13-t-Bu upregulated the levels of pro- and cleaved forms of caspase-3, -8, and -9 and Bcl- 2. Phospho-STAT3, phospho-Src, and phospho-AKT levels were also diminished by KTH13-t-Bu treatment. Therefore, these results strongly suggest that KTH-13-t-Bu can be considered a novel anti-cancer drug displaying pro-apoptotic activity.

• Proceedings of the Polymer Society of Korea Conference
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• 2006.10a
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• pp.121-122
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• 2006

Paralleled with the development of new materials we need to develop methods and techniques to reveal the environmental interaction and impact of the new materials. Small changes in the chemical structure or product formulation may render the product less environmentally adaptable. Degradation products formed from PLLA were identified after aging in different environments and their assimilation in the biotic environment was shown. Green and degradable hydrogels could be designed from renewable hemicelluloses and lactic acid. Hemicelluloses are a renewable and highly interesting raw material source for new green polymers.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2020.11a
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• pp.224-225
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• 2020

TV홈쇼핑은 Full HD로 방송이 제작되고 있으며, 방송제작 시 스튜디오 스텐다드 카메라, jimmy jib 카메라 외에 추가로 다양한 카메라가 방송제작에 활용된다. 다양한 카메라 중 4K 카메라를 스튜디오 제작에 활용하여 제작의 퀄리티를 높이고 있다. 4K 카메라는 제작 회사 마다의 특성에 따라 각각 다른 화질과 다양한 기능을 제공한다. 본 논문은 4K 카메라의 여러 기능 중 고속촬영 기능의 화질 비교에 관한 것이다. 홈쇼핑 방송제작에 활용되는 4K 카메라의 다양한 활용성을 제시하고자 한다.

• Biomolecules & Therapeutics
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• v.24 no.4
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• pp.402-409
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• 2016

It has been found that 4-isopropyl-2,6-bis(1-phenylethyl)phenol (KTH-13), a novel compound isolated from Cordyceps bassiana, is able to suppress tumor cell proliferation by inducing apoptosis. To mass-produce this compound, we established a total synthesis method. Using those conditions, we further synthesized various analogs with structural similarity to KTH-13. In this study, we aimed to test their anti-cancer activity by measuring anti-proliferative and pro-apoptotic activities. Of 8 compounds tested, 4-methyl-2,6-bis(1-phenylethyl)phenol (KTH-13-Me) exhibited the strongest anti-proliferative activity toward MDA-MB 231 cells. KTH-13-Me also similarly suppressed the survival of various cancer cell lines, including C6 glioma, HCT-15, and LoVo cells. Treatment of KTH-13-Me induced several apoptotic signs in C6 glioma cells, such as morphological changes, induction of apoptotic bodies, and nuclear fragmentation and chromatin condensation. Concordantly, early-apoptotic cells were also identified by staining with FITC-Annexin V/PI. Moreover, KTH-13-Me highly enhanced the activation of caspase-3 and caspase-9, and decreased the protein level of Bcl-2. In addition, the phosphorylation levels of Src and STAT3 were diminished in KTH-13-Me-treated C6 cells. Therefore, these results suggest that KTH-13-Me can be developed as a novel anti-cancer drug capable of blocking proliferation, inducing apoptosis, and blocking cell survival signaling in cancer cells.

• Journal of Korean Tunnelling and Underground Space Association
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• v.11 no.4
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• pp.373-385
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• 2009

A new indexing methodology so called KTH-index was developed to quantitatively evaluate a potential level for tunnel collapse hazard, which has been successfully applied to tunnel construction sites to date. In this study, an attempt is made to apply this methodology for validating an outcome of tunnel design by checking the variation of KTH-index along longitudinal tunnel section. In this KTH-index simulation, it is the most important to determine the input factors reasonably. The design factor and construction condition are set up based on the designed outcome. Uncertain ground conditions are arranged based on borehole test and electro-resistivity survey data. Two scenarios for ground conditions, best and worst scenarios, are set up. From this simulation, it is shown that this methodology could be successfully applied for providing quantitative validity of a tunnel design and also potential hazard factors which should be carefully monitored in construction stage. The hazard factors would affect sensitively the hazard level of the tunnel site under consideration.

• Proceedings of the Korean Magnestics Society Conference
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• 2008.12a
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• pp.280.2-280.2
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• 2008

• Proceedings of the Korean Magnestics Society Conference
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• 2008.12a
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• pp.304.2-304.2
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• 2008

• Biomolecules & Therapeutics
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• v.23 no.4
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• pp.367-373
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• 2015

Cordyceps species including Cordyceps bassiana are a notable anti-cancer dietary supplement. Previously, we identified several compounds with anti-cancer activity from the butanol fraction (Cb-BF) of Cordyceps bassiana. To expand the structural value of Cb-BF-derived anti-cancer drugs, we employed various chemical moieties to produce a novel Cb-BF-derived chemical derivative, KTH-13-amine-monophenyl [4-isopropyl-2-(1-phenylethyl) aniline (KTH-13-AMP)], which we tested for anti-cancer activity. KTH-13-AMP suppressed the proliferation of MDA-MB-231, HeLa, and C6 glioma cells. KTH-13-AMP also dose-dependently induced morphological changes in C6 glioma cells and time-dependently increased the level of early apoptotic cells stained with annexin V-FITC. Furthermore, the levels of the active full-length forms of caspase-3 and caspase-9 were increased. In contrast, the levels of total forms of caspases-3, caspase-8, caspase-9, and Bcl-2 were decreased in KTH-13-AMP treated-cells. We also confirmed that the phosphorylation of STAT3, Src, and PI3K/p85, which is linked to cell survival, was diminished by treatment with KTH-13-AMP. Therefore, these results strongly suggest that this compound can be used to guide the development of an anti-cancer drug or serve as a lead compound in forming another strong anti-proliferative agent.

• Proceedings of the Korean Geotechical Society Conference
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• 2010.09a
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• pp.1294-1301
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• 2010

In this study, a case study, where a hazard management for tunnel collapses has been quantitatively undertaken based on the KICT Tunnel Hazard(KTH) index, is presented. From this, it was able to timely inform the field engineers when the more detailed investigation is required for checking if any risky factor is shown on the tunnel face. At the same time, variable additional information such as sensitivities of major influence factors are also provided to field engineers from the methodology given in this study. The additional information would be helpful for better understanding of tunnel hazard level at the current tunnelling stage and following the required actions for more detailed checks of risky factors.

• Animal cells and systems
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• v.14 no.3
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• pp.169-174
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• 2010

Cutlassfish (Trichiurus lepturus Linnaeus 1758) is a commercially important fish in Korea. In recent years, the catch of cutlassfish in the coastal waters of Korea has significantly declined. Its genetic characterization has been little studied. To assist conservation and management efforts, we isolated and characterized 10 microsatellite loci using an enrichment method based on magnetic/biotin capture of microsatellite sequences from a size-selected genomic library. To characterize each locus, 30 individuals from a natural T. lepturus population in the coastal waters of Jeju Island, Korea, were genotyped. All loci except two, KTh9B and KTh22A, were polymorphic, with an average of 14.3 alleles per locus (range, 10 22). The mean observed and expected heterozygosities were 0.80 (range, 0.50 0.97) a 0.82 (range, 0.68 0.95), respectively. A significant deviation from Hardy-Weinberg equilibrium was observed at three loci (KTh6B, KTh10, and KTh16). This high variability indicates that these microsatellites may be useful for high-resolution studies of population genetics.