• Journal of the Korean Society of Tobacco Science
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• v.28 no.1
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• pp.51-57
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• 2006

This study was carried out to improve and evaluate the analytical method for determination of potassium in tobacco leaf by various pre-treatment techniques. The time requirment of various pre-treatments was about 10 hour for dry ashing and 6 hour for microwave digestion and 3 hour for sonication. The results of recover in both pre-treatment techniques, microwave digestion and sonication, is greater than 85 % stably with reproducibility(RSD %) on replicates of less than 3 %. However the mean values for microwave digestion were lower than certified standard value of NlST SRM. Compared to the other pre-treatment techniques, analytical results for sonication technique strictly improved the accuracy and precision. In conclusion, the use of the simple sonication technique seems to be efficient for the determination of potassium in tobacco leaf in consideration for both the accuracy and reproducibility.

• Journal of the Korean Society of Tobacco Science
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• v.28 no.1
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• pp.31-35
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• 2006

In this study, the effects of the relative humidity and storage time of blended tobaccos at total blending silo on cut tobacco equilibrium moisture contents, leaf moisture contents and migration of casing materials of tobacco types were investigated. To maintain the cutting moisture content(21 $\pm$ 1 %), it is necessary to keep 75 % relative humidity at 28 $^{\circ}C$ at total blending silo. The moisture content of reconstituted tobacco sheet was changed from $13{\sim}14$ % to $19{\sim}21$ % within 2 h after preblending. The contents of glycerin and fructose of tobacco types followed by storage time after preblending were not changed significantly. From these results, it is suggested that 2 h storage time after preblending was sufficient to maintain physical properties of cut tobaccos and tobacco taste and fragrance.

• Journal of the Korean Society of Tobacco Science
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• v.28 no.1
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• pp.17-22
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• 2006

This study was conducted to investigate the effect of soil, cultural practices and climatic conditions on some chemical constituents of flue-cured tobacco. Increasing the nicotine and total nitrogen contents may be useful to reduce the total sugar content of cured leaves in flue-cured tobacco. Delaying the transplanting date and increasing the soil nitrogen($N0_3-N$ and $N0_4-N$) content for 30 days after transplanting by fertilizing are desirable so as to increase the nicotine and total nitrogen contents of cured leaves. Those treatments will delay the ripeness, and elongate the duration of cultivation(day from transplanting to harvesting), and increase the fresh leaf weight. Moderate rainfall in April and May, lower relative humidity in June, and higher mean daily air temperature in June and July seem to be necessary for good leaf of flue-cured tobacco in Korea.

• Journal of the Korean Society of Tobacco Science
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• v.27 no.2
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• pp.189-194
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• 2005

The morphological properties of various structure materials of domestic and foreign reconstituted tobacco products(RTP) were investigated by using the Bauer-McNett classifier and the image analyzer. The results of the fiber classification showed the fraction of the bigger size structure materials was larger in a domestic RTP than that in two foreign RTPs. In case of fine fraction, the domestic RTP had bigger fine fraction than two foreign RTPs. Images of each structure materials showed the scrap in the foreign RTPs kept the original shape which were rare in the domestic RTP fractions. Those results deduced that the raw materials in a foreign RTP process might be treated separately depending on the mechanical and morphological properties, which could reduce the amount of fine generation and increase the efficiency in raw material treatment.

• Journal of the Korean Society of Tobacco Science
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• v.27 no.2
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• pp.219-225
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• 2005

Characterizing nicotine delivery from tobacco products is important in the understanding of their addictive potential. Most previous studies report total nicotine and have not differentiated between nicotine in its protonated or free-base form. The amount of free nicotine calculated by determining pH and nicotine contents. The pH and nicotine contents in smokeless tobacco product, tobacco products and tobacco leaves were analyzed by Health Canada-Official Method T-310 and CORESTA Recommended Method $N^{\circ}62$. The content of free nicotine was calculated according to the Henderson-Hasselbalch equation and the value of $\alpha_{fb}$(the fraction of nicotine that is in the free base form) by using a pKa value of 8.02 for nicotine. The percentage of free nicotine then was calculated by dividing the free nicotine content by total nicotine content. The pH value and percentage of free nicotine ranged from 5.01 to 5.45 and $0.10\%\;to\;0.27\%$ in cut tobacco and 5.10 to 7.10 and $0.12\%\;to\;10.73\%$ in tobacco leaves, respectively.

• Journal of the Korean Society of Tobacco Science
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• v.31 no.1
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• pp.58-67
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• 2009

Biomarkers could be critical and useful tools for assessing the biological effects of smoking and detecting differences between potentially reduced exposure product (PREP) and conventional cigarettes. Smoking-related biomarkers can be classified into three categories as biomarkers of exposure, biomarkers of effects, and biomarkers of potential harm. When compared with the biomarkers of effects or harm, the biomarkers of exposure for chemical constituents of cigarette smoke are well established and characterized. In addition, they could offer the important information in understanding how cigarette smoke interacts with biological molecules and causes the disease to human. Therefore, we provide an overview of 6 biomarkers of exposure (Nicotine and nicotine metabolites, Carboxyhaemoglobin, NNAL (4-(methylnitrosoamino)-1-(3-pyridyl)-1-butanol) and NNAL - glucuronide, 3-Hydroxypropyl-mercapturic acid, and Monohydroxy-butenyl-mercapturic acids, and Urine mutagenicity) which were validated through extensive research and clinical experience. These reliable biomarkers could help identify the efficacy of PREP by predicting early toxicological effects and lead to improve it.

• Journal of the Korean Society of Tobacco Science
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• v.31 no.1
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• pp.9-17
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• 2009

Activated carbon fiber (ACF) was surface modified by nitric acid to improve the adsorption efficiency of the propylamine. Functional groups and textural properties of modified ACF were investigated. The total surface acidity increased about 7 times to that of as-received ACF by modification with 1 M nitric acid solution, carboxylic and phenolic groups mainly increased. However, the specific surface areas and the total pore volumes of the modified ACFs were decreased by 5-8% due to the increased blocking (or demolition) of micropores in the presence of newly introduced complexes. Despite the decrease of textural properties, it was found that the amount of propylamine adsorbed by the modified ACFs was increased by approximately 17%. The oxygen and nitrogen contents on the modified ACF increased by 1.5 and 3 times compared with the as-received ACF. From the XPS results, it was observed that propylamine reacted with strong or weak acidic groups, such as -COOH or -OH on the ACF surfaces, resulting in the formation of pyrrolic-, pyridonic- or pyridine-like structures.

• Journal of the Korean Society of Tobacco Science
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• v.29 no.2
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• pp.118-124
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• 2007

GRLs(Guidance Residue Levels) of agricultural chemicals for tobacco are recommended by the CORESTA Agro-Chemical Advisory Committee guide. In the GRLs list, organochlorine group is one of pesticides commonly used on tobacco cultivation. In this model study, the quantitative correlation in the transfer rate of pesticide residue into tobacco smoke by spiking of organochlorine pesticides to cigarette and pyrolysates were investigated. The spiking concentration referred to the range of GRLs list and the organochlorine pesticides in mainstream smoke were analyzed by GC-MS. For the understanding of the composition variation versus temperature, the behavior of pesticides was investigated by pyrolysis-gas chromatography-mass spectrometry(Py-GC-MS). In this study, the transfer rate of pesticide residue into tobacco smoke at four different spiking concentration and the composition of pyrolysates were analyzed differently. At $10\;{\mu}g/cig$ spiking concentrations, the organochlorine pesticides were transferred into tobacco smoke in $0.02\;{\sim}\;10.19\;%$ each of component and the most of pesticides were pyrolyzed during smoking. It was found that the decomposition compounds from organochlorine pesticides were mainly composed of oxygenated and nitrogenous compounds. This study could estimate that the transfer rate of pesticides into tobacco smoke is very small amount.

• Journal of the Korean Society of Tobacco Science
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• v.29 no.2
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• pp.104-109
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• 2007

The amount of nitrate in the tobacco leaf has been shown to be correlated with the levels of alkaloids and nitrosamines. Also the nitrate content of the tobacco correlated closely with the smoke delivery of nitric oxide and tobacco-specific nitrosamines (TSNAs). These are related with the effect of the reconstituted tobacco leaf(Recon) using the tobacco stems. Adsorption process is gaining interest as one of the effective processes of advanced liquid treatment for liquid containing unnecessary materials. This study is focused on the evaluation of four anion exchangers, a cation exchanger and an activated carbon, as adsorbents for reduction of nitrate anion from Recon extract. In order to analyze the nitrate anion, the IC method used in this work was carried out with a Dionex ICS-2000 system. The effects of dosages of adsorbents and concentration of extract on the removal of nitrate anion were examined. Experimental results showed that for nitrate-anion exchanger, nitrate-cation exchanger and nitrate-activated carbon adsorption system, approximately 70 %, 10 %, and 4 % removal efficiencies were achieved at the Brix 10 and the 20 % addition. Although the activated carbon was little efficient for removal of nitrate ion, the removal of nicotine was very efficient at given conditions.

• Journal of the Korean Society of Tobacco Science
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• v.29 no.2
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• pp.80-84
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• 2007

This study was conducted to evaluate the prediction of sensory property of smoke from the leaf chemical property and characterize leaf chemical components for the best tobacco taste's leaves in burley tobacco. For analytical and sensory evaluations, sixteen grades were used. The major leaf chemical components to predict the sensory property of smoke were ether extract for tobacco-like, chloride for impact and total nitrogen/nicotine for irritation. Within ${\pm}20\;%$ range of difference, the predictable probabilities of sensory property of smoke from the leaf chemical properties were 100 % for tobacco-like, impact and irritation. As a result of K-means cluster analysis on the basis of tobacco taste, the desirable leaf chemical component contents were $6.5{\sim}6.8\;%$ in ether extract, $0.25{\sim}0.30\;%$ in chloride and $1.26{\sim}1.54$ in total nitrogen/nicotine ratio. This study suggest that the some regression equations may be useful to predict the sensory components of tobacco smoke from a few selected leaf chemical properties in burley tobacco and to select the burley tobacco leaves for enhance the tobacco taste of cigarette.