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Calculation of Free Nicotine by Determination of pH and Nicotine in Tobacco  

Lee Jeong-Min (KT&G Central Research Institute)
Jang Gi-Chul (KT&G Central Research Institute)
Hwang Keon-Joong (KT&G Central Research Institute)
Kim Yong-Ha (KT&G Central Research Institute)
Rhee Moon-Soo (KT&G Central Research Institute)
Publication Information
Journal of the Korean Society of Tobacco Science / v.27, no.2, 2005 , pp. 219-225 More about this Journal
Abstract
Characterizing nicotine delivery from tobacco products is important in the understanding of their addictive potential. Most previous studies report total nicotine and have not differentiated between nicotine in its protonated or free-base form. The amount of free nicotine calculated by determining pH and nicotine contents. The pH and nicotine contents in smokeless tobacco product, tobacco products and tobacco leaves were analyzed by Health Canada-Official Method T-310 and CORESTA Recommended Method $N^{\circ}62$. The content of free nicotine was calculated according to the Henderson-Hasselbalch equation and the value of $\alpha_{fb}$(the fraction of nicotine that is in the free base form) by using a pKa value of 8.02 for nicotine. The percentage of free nicotine then was calculated by dividing the free nicotine content by total nicotine content. The pH value and percentage of free nicotine ranged from 5.01 to 5.45 and $0.10\%\;to\;0.27\%$ in cut tobacco and 5.10 to 7.10 and $0.12\%\;to\;10.73\%$ in tobacco leaves, respectively.
Keywords
free nicotine; nicotine; pH; Henderson-Hasselbalch equation;
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  • Reference
1 CDC (1999) Determination of nicotine, pH and moisture content of six US commercial moist snuff products. MMWR Weekly 48(19): 398-401
2 Pankow, J.F. (2001) A consideration of the role of gas/particle partitioning in the deposition of nicotine and other tobacco smoke compounds in the respiratory tract. Chem. Res. Toxicol. 14(11): 1465-1481   DOI   PUBMED   ScienceOn
3 Pankow, J.F., Barsanti, K.C. and Peyton, D.H. (2003) Fraction of free-base nicotine in fresh smoke particulate matter from the Eclipse 'cigarette' by $^{1}H$ NMR spectroscopy. Chem. Res. Toxicol. 16: 23-27   DOI   ScienceOn
4 Williamson R.E. and Chaplin J.F. (1981) Levels of chemical constituents in cured leaves of four burley tobacco cultivars according to stalk position. Tobacco Science 25: 75-78
5 Rogers, J.D. and Mitchem A.R. (1976) Physical properties of leaf as indicators of chemical and smoking properties. Recent Advanced in Tobacco Science 2: 112-126
6 Akehurst B. C. (1981) Tobacco. p. 589, 649. 2nd ed., Longman Groupe Limited, Harlow U.K
7 WHO (2003) WHO Framework Convention On Tobacco Control, World Health Organization, Geneva, Switzerland
8 Morie, GP (1972) Fraction of protonated and unprotonated nicotine in tobacco smoke at various pH values. Tobacco Science 16: 167
9 Pankow, J.F., Mader, B.T., Isabelle, L.M., Luo, W., Pavlick, A. and Liang, C. (1997) Conversion of nicotine in tobacco smoke to its volatile and available free-base form through the action of gaseous ammonia. Environ. Sci. Technol. 31: 2428-2433   DOI   ScienceOn
10 Pankow, J.F., Tavakoli, A.D., Luo, W. and Isabelle, L.M. (2003) Percent free base nicotine in the tobacco smoke particulate matter selected commercial and reference cigarettes. Chem. Res. Toxicol. 16: 1014-1018   DOI   ScienceOn
11 Cochran, E.W., Joseph, M.J., Stinson, S.L. and Summers, S.S. (2003) Application of a diffusion-denuder method for the investigation of the effects of 'smoke pH' on vapor-phase nicotine yields from different types of cigarettes. Beitr. Tabakforsch. Int. 2(6): 365-372
12 CORESTA (2005) Determination of nicotine in tobacco and tobacco products by gas chromatographic analysis. CORESTA Recommended Method N$^{\circ}$62
13 Health Canada (1999) Determination of whole tobacco pH. Health Canada-Official Method T-310
14 WHO (2004) WHO Library Cataloguing-in Publication Data, World Health Organization, Geneva, Switzerland
15 Moore, K. (1993) Calculations of free-Base and protonated forms of nicotine, Tobacco Document On-line RJR-508728766-508728768
16 Watson, C.H., Trommel, J.S., and Ashley, D.L. (2004) Solid-phase microextraction-based approach to determine free-base nicotine in trapped mainstream cigarette smoke total particulate matter. J. Agric. Food Chem. 52: 7240-7245   DOI   ScienceOn
17 Whidby, J.F. and Seeman, J.I. (1976) The configuration of nicotine. A nuclear magnetic resonance study. J. Org. Chem. 41: 1585-1590   DOI   PUBMED