• IMMUNE NETWORK
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• 제10권6호
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• pp.212-218
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• 2010

Backgroud: The stem bark of Kalopanax pictus (KP) has been used in traditional medicine to treat rheumatoidal arthritis, neurotic pain and diabetes mellitus in China and Korea. In this study, the mechanism responsible for anti-inflammatory effects of KP was investigated. Methods: We examined the effects of KP on NO production, nitric oxide synthase (iNOS) and HO-1 expression, NF-${\kappa}B$, Nrf2 and MAPK activation in mouse peritoneal macrophages. Results: The aqueous extract of KP inhibited LPS-induced NO secretion as well as inducible iNOS expression, without affecting cell viability. KP suppressed LPS-induced NF-${\kappa}B$ activation, phosphorylation and degradation of $I{\kappa}B-{\alpha}$, phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) and c-Jun N-terminal kinase (JNK). Furthermore, KP induced HO-1 expression and Nrf2 nuclear translocation. Conclusion: These results suggest that KP has the inhibitory effects on LPS-induced NO production in macrophages through NF-${\kappa}B$ suppression and HO-1 induction.

• 한국가축번식학회지
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• 제22권4호
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• pp.405-410
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• 1998

본 연구는 독자 개발한 돼지액상정액희석제 Kp(한국생명과학연구소)에 pH를 안정화하여 보존 중 정자의 운동성을 유지시킬 수 있도록 EDTA, Tris, Citrate buffer의 적정 첨가농도를 결정하고자 실시하였다. Basic Kp와 BTS (Mini-tube, Germany; BT-Sg), BTS (Tri-bio, USA; BTSa), Modena (SGI, USA)를 17$^{\circ}C$ 에서 각각 보관한 경우 모든 희석액에서 시간 경과에 따라 pH가 증가하였고, Basic Kp 는 희석 당일의 pH가 다른 희석액에 비해 높게 나타났는데 이것은 돼지액상정액의 생리적인 pH인 6.8~7.5에 비해서도 높은 수준이었다. 정액내의 pH 저하를 방지해 주고 정액의 생리적인 pH를 유지하기 위하여 Basic Kp에 EDTA, Tris, Citrate buffer를 단계적으로 첨가하면서 시간 경과에 따른 pH 변화를 살펴 본 결과 1.25g /L EDTA, 1.42g /L Tris, 1.00g /L Citrate를 첨가한 경우 (Modified Kp) pH는 l일째 6.88에서 6일째 7.33으로 유지되었다. 특히, Modified Kp에 첨가된 buffer 의 농도는 Modena와 다른 희석제에 첨가된 농도에 비해 1/2 에서 1/4 정도로 낮은 수준이었다. Modified Kp 와 Basic Kp, BTSg, BTSa 및 Modena로 냉동-융해된 돼지 정자를 희석하여 보존한 경우 정자의 운동성은 Modified Kp가 다른 희석액에 비해 유의하게 높게 나타났다(87.0% vs. 71.0~48.0% in day 1; 13.3% vs. 6.3~0% of day 6), 이상의 결과를 종합해 볼 때, Modified Kp는 낮은 농도의 EDTA, Tris, Citrate buffer 첨가에도 불구하고 돼지정자의 생리적인 pH 수준을 잘 유지할 수 있었으며, 수입되어 냉동-융해된 돼지 정자에 사용하는 희석제 BTSg, BTSa, Modena 보다도 정자의 운동성에 효과적이었다.

• Journal of Microbiology
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• 제42권2호
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• pp.74-79
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• 2004

Ingestion of shellfish-associated Vibrio parahaemolyticus is the primary cause of potentially severe gas-troenteritis in many countries. However, only Kanagawa phenomenon (hemolysin) positive (KP$\^$+/) strains of V. parahaemolyticus are isolated from patients, whereas >99％ of strains isolated from the environment do not produce this hemolysin (i.e. are KP$\^$-/). The reasons for these differences are not known. Following a temperature downshift, Vibrio parahaemolyticus enters the viable but noncultur-able (VBNC) state wherein cells maintain viability but cannot be cultured on routine microbiological media. We speculated that KP$\^$+/ and KP$\^$-/ strains may respond differently to the temperature and salinity conditions of seawater by entering into this state which might account for the low numbers of cul-turable KP$\^$+/ strains isolated from estuarine waters. The response of eleven KP$\^$+/ and KP$\^$-/ strains of V. parahaemolyticus following exposure to a nutrient and temperature downshift in different salinities, similar to conditions encountered in their environment, was examined. The strains included those from which the KP$\^$+/ genes had been selectively removed or added. Our results indicated that the ability to produce hemolysin did not affect entrance into the VBNC state. Further, VBNC cells of both biotypes could be restored to the culturable state following an overnight temperature upshift.

• Asian-Australasian Journal of Animal Sciences
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• 제26권8호
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• pp.1080-1088
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• 2013

Our previous results showed that kisspeptin-10 (Kp-10) injections via intraperitoneal (i.p.) once daily for three weeks notably promoted the egg laying rate in quails. In order to investigate the mechanism behind the effects of Kp-10 on enhancing the egg laying rate in birds, this study focused on the alternations of lipids synthesis in liver after Kp-10 injections. 75 female quails (22 d of age) were allocated to three groups randomly, and subjected to 0 (control, Con), 10 nmol (low dosage, L) and 100 nmol (high dosage, H) Kp-10 injections via i.p. once daily for three weeks, respectively. At d 52, quails were sacrificed and sampled for further analyses. Serum $E_2$ concentration was increased by Kp-10 injections, and reached statistical significance in H group. Serum triglyceride (TG) concentrations were increased by 46.7% in L group and 36.8% in H group, respectively, but did not reach statistical significance, and TG contents in liver were significantly elevated by Kp-10 injections in a dose-dependent manner. Serum total cholesterol (Tch) concentrations significantly decreased in H group, while in H group the hepatic Tch content was markedly increased. The level of non-esterified fatty acid (NEFA), apolipoprotein A1 and B (apoA1 and apoB) were not altered by Kp-10 injections. The genes expression of sterol regulatory element binding protein-1 (SREBP-1), fatty acid synthetase (FAS), apolipoprotein VLDL-II (apoVLDL-II), cholesterol $7{\alpha}$-hydroxylase (CYP7A1) and vitellogenin II (VTG-II) were significantly up-regulated by high but not low dosage of Kp-10 injection compared to the control group. However, the expression of SREBP-2, acetyl-CoA carboxylase ($ACC_{\alpha}$), malic enzyme (ME), stearoyl-CoA (${\Delta}9$) desaturase 1 (SCD1), apolipoprotein A1 (apoA1), fatty acid binding protein 2 (FABP2), 3-hydroxyl-3-methyl glutaryl-coenzyme A reductases (HMGCR), estrogen receptor ${\alpha}$, ${\beta}$($ER{\alpha}$ and ${\beta}$) mRNA were not affected by Kp-10 treatment. In line with hepatic mRNA abundance, hepatic SREBP1 protein content was significantly higher in H group. Although the mRNA expression was not altered, the content of $ER{\alpha}$ protein in liver was also significantly increased in H group. However, SREBP-2 protein content in liver was not changed by Kp-10 treatment. In conclusion, exogenous Kp-10 consecutive injections during juvenile stage significantly advanced the tempo of egg laying in quails, which was associated with the significant elevation in hepatic lipids synthesis and transport.

• Journal of Animal Science and Technology
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• 제46권3호
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• pp.301-306
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• 2004

돼지 6번 염색체에서 근내지방 함량과 등지방 두께와 관련된 QTL이 탐색되어진 영역(6q28-6q32)에서 미세지도 작성을 위한 유용한 marker를 개발하기 위하여 실시하였다. 대량 염기서열 분석자료를 근거로, 반복염기서열 분석을 수행한 결과 KP0290F2(TTCC), KP0248C11(AAAT), KP1231C91(TAG), KP1231C92(TTG) 그리고 KP1231C93(GA)의 5 부위에서 다형성을 나타내었다. 이 부위들에 대한 랜드레이스, 재래돼지, 듀록, 요크셔, 버크셔, 오지산돈, 향돈 그리고 민돈의 8품종에 대한 유전자형 분석 결과 평균 대립유전자의 수는 2.13, 4.63, 7.38, 2.75 그리고 6.25로 나타났다. 그리고 8품종에 대한 KP0290F2, KP0248C11, KP1231C91, KP1231C92 그리고 KP1231C93에 대한 평균 heterozygosity 값을 산출한 결과, 0.2110, 0.6865, 0.8304, 0.4057 그리고 0.7051로 나타났으며, 5markers에 대한 8 품종의 평균 heterozygosity 값은 0.6313, 0.5662, 0.5814, 0.6957, 0.4517, 0.4847, 0.5758 그리고 0.5559로 나타났다. KP0248C11, KP1231C91 그리고 KP1231C93은 적절한 대립유전자 수를 나타내었고, 또한 hetetozygosity 값이 높게 나타났을 뿐만 아니라, 표준편차도 적게 나타난 점으로 미루어 보아 앞으로 유용한 marker로서 이용이 가능할 것이라 사료된다. 본 연구의 결과 개발된 marker는 SW71(98.6cM)과 SW1881(121.1 cM) 영역내에 존재하는 유용한 유전자를 발굴하기 위한 미세지도 작성에 유용하게 활용될 수 있는 marker로 판단되며, positional cloning에도 이용 할 수 있을 것이라 사료된다. 또한 돼지 게놈 연구가 완성되어 염기배열이 밝혀지기 전에 이용 가능한 표지인자들을 다량으로 확보 수 있을 것이라 사료된다.

• 대기
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• 제18권1호
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• pp.43-53
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• 2008

A new proposition on the definition of the tropical cyclone (TC) which influences the Korean Peninsula (KP) is presented. The definition is based upon the TC track and intensity, 34 wind swath considering the TC size, and the line of 200 nautical mile (NM) from the KP shore which is the boundary of the official warning of Korea Meteorological Administration. Four types are proposed. First type is TC that hits the KP inland. Second is TC that falls within the 200-NM boundary. Third type is TC that passes outside the 200-NM boundary but large enough to significantly influence the KP. Last, the cases for a TC which are downgraded to the midlatitude cyclone and hit the KP are included. 30-year reanalysis reveals that 21 tropical cyclones should be included in the TC list that influenced the KP during the period from 1977 to 2006, which corresponds to 3.93 TCs per year. Among them, number of type I, II, III and IV TCs turn are to be 36, 47, 10, and 16, respectively. The net increase found in the current reanalysis is 2, 5, 7, and 7 for each type.

• 한국정보기술학회 영문논문지
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• 제10권1호
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• pp.45-56
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• 2020

Despite extensive research, performance enhancement of keyphrase (KP) extraction remains a challenging problem in modern informatics. Recently, deep learning-based supervised approaches have exhibited state-of-the-art accuracies with respect to this problem, and several of the previously proposed methods utilize Bidirectional Encoder Representations from Transformers (BERT)-based language models. However, few studies have investigated the effective application of BERT-based fine-tuning techniques to the problem of KP extraction. In this paper, we consider the aforementioned problem in the context of scientific articles by investigating the fine-tuning characteristics of two distinct BERT models - BERT (i.e., base BERT model by Google) and SciBERT (i.e., a BERT model trained on scientific text). Three different datasets (WWW, KDD, and Inspec) comprising data obtained from the computer science domain are used to compare the results obtained by fine-tuning BERT and SciBERT in terms of KP extraction.

• 해운
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• 11호통권10호
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• pp.2-13
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• 2004

• 해운
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• 3호통권14호
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• pp.2-9
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• 2005

• 해운
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• 12호통권23호
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• pp.30-30
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• 2005