• Korean Journal of Poultry Science
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• v.23 no.2
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• pp.61-69
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• 1996

Primordial germ cells (PGCs) were manipulated as part of the system to produce transgenic chickens. PGCs were isolated from the germinal crescent of developmental stage 6 to 8 donor emhryos of the Korean Native Ogol Chickens (KNOC). These PGCs were transfected with plasmid DNA containing the lac Z gene by liposome mediated transfection methods. The lac Z gene was transfected and expressed in the PGCs. These transfected PGCs were injected into the germinal crescent of White Leghorn embryos (stage 6 to 8). The injected transfected PGCs migrated via the circulatory system into the future gonad and expression observed in the gonads of 3 day old chick. Of the 47 embryos and 3 day old chickens, one positive PGCs gonad from sacrificed young chickens was detected by appearance of blue cells. Plasmid DNA with the foreign gene was incorporated into the population of germ cells in the gonad. These results demonstrate that PGCs can he transfected and then transferred for colonization into the gonad, and show the potential to ultimately manipulate the genetic material of the chicken gernline.

• Proceedings of the Korea Society for Simulation Conference
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• 2002.05a
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• pp.21-32
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• 2002

In accordance with the development plans of Ulsan harbor, Ulsan new harbor will be contructed considering supporting Ulsan harbor as a safe berthing and departure at single buoy mooring(SBM). In this study, we used a full-mission ship handling simulator adopting 300,000 DWT VLCC manoeuvered at the planned Ulsan SBM. Five masters who have had a long experience of ship maneuvering were called to carry out the simulations, of which each scenario were tried one, completed total of 68 times. The marine traffic safety was assessed in terms of 1) the closest point of approach(CPA) to other SBM and breakwater in the vicinity and the probability of crossing the restricted area of the closest SBM and fairway limit, 2) subjective evaluation such as the mental burden and the maneuvering difficulty of shiphanders, and 3) the opinions of shiphandlers. From the result of this simulation, we have a conclusion as follows; First, because crude oil berthing angle is so small by current S-OiL Co. crude oil buoy by SK Co. No 3 crude oil buoy different view SK Co. No 3 crude oil buoy and interference of current KNOC crude oil buoy, Berthing is impossible, and Emergency departing is very dangerous too operation impossible. Second it is desirable that SK Co. No 2 and No 3 Single buoy Mooring that do different view controls position so that to be not put in straight line each other. Third, SK Co. No 1 and 2 single buoy mooring that do different view to Onsanhang berthing and departing is seized by single buoy mooring by external force ship that set sail does faith control need.

• Geophysics and Geophysical Exploration
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• v.2 no.3
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• pp.149-153
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• 1999

The study was performed as a part of 3-D exploration project of the South Con Son basin, where Korea National Oil Co. (KNOC) and SHELL Company are performing joint operation. In the structurally complex area, seismic facies or lap-out patterns, which are usually the tools for the conventional seismic stratigraphy developed by Exxon Group (Vail et at., 1977), are not easily identifiable. Therefore, stratigraphic informations are mainly extracted from seismic attribute maps of each sequence or systems tracts, and isopach maps in correlation with the stratigraphic information from the wells. The attribute maps of the sequence or systems tract boundaries and isopach map describe the variations of paleodepositional environments. The shape of the attribute maps of the boundaries is a reasonable description of the shape of the paleodepositional surface. With other maps such as isopach and structural maps, the variations of the parasequences in the systems tracts can be projected using the surface attribute maps. The reflection intensity attribute at each sequence or system tract boundary can be related to lithology, facies or porosity distributions. The azimuth attribute of source rock sequence can be used to identify the hydrocarbon migration patterns into the prospects. The overall risks of reservoir rocks, cap rocks, structure and hydrocarbon migrations were computed using the results of the study.

• Economic and Environmental Geology
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• v.48 no.4
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• pp.301-312
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• 2015

A commercial gas field was found in the southwestern continental shelf of the Ulleung Basin, East Sea in the late 1990s. To develop additional gas field, an exploration well was drilled through the coarse infill of submarine canyon near the gas field, but it was uneconomic to develop hydrocarbons. Using newly acquired deep seismic reflection and previous well data, we have identified additional geological structure which has hydrocarbon potentials below submarine canyons in the southwestern margin of the basin. Based on the interpretation of the deep seismic reflection and well data, the sequences of the study area can be classified into the syn-rift megasequence(MS1), post-rift megasequence(MS2), syn-compressional megasequence(MS3), and post-compressional megasequence(MS4) in relation to the tectonic events. MS1, deposited simultaneously with the basin formation before the middle Miocene, is characterized by chaotic seismic facies with low- to moderate-amplitude and low frequency reflections. MS2 comprises laterally continuous, low- to moderate-amplitude reflections, showing progradational stacking patterns due to high rates of sediment supply during basin expansion in the middle Miocene. MS3 is mainly composed of continuous reflections with high amplitude and moderate- to high-frequency which are interpreted as coarse-grained sediments. The coarse-grained sediments of MS3 sequence is widely truncated by several submarine canyons which filled with fine-grained sediment of MS4 to form a stratigraphic trap of hydrocarbon. Therefore, the reservoir and seal of the hydrocarbon trap in the study area are coarse-grained sediment of MS3 and submarine canyon filled with fine-grained sediment of MS4, respectively. A flat-spot seismic anomaly, which may indicate the presence of hydrocarbon, is observed within the stratigraphic trap.

• The Korean Journal of Petroleum Geology
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• v.11 no.1 s.12
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• pp.1-8
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• 2005

Seventeen exploratory wells have been drilled in the Block VI-1 of offshore Korea, which is located in the southern part or the Ulleung Basin. Gas show has been recognized from most of the wells, and gas and condensate have been accompanied in some wells. Commercial discovery of gas, accompanied by condensate, has been made from Gorae V well. The reservoir gases or the Dolgorae III, Gorae I, and Gorae V wells in the Ulleung Basin mainly consists of hydrocarbon gases (>93%). These gases are thermogenic wet gases which contain more than 96% of the methane and result from the cracking of petroleum or kerogen. Based on the chemistry and composition of the gases and stable isotope data, they seem to be generated from different source rocks. The condensates from the Gorae I and V wells are mostly generated from terrestrial organic matter. Lacustrine organic matter may not play an important role for the generation of these condensates. The condensates from the Gorae V wells consist predominantly of terrestrial organic matter but with minor subsidiary input from marine organic matter. The condensates from Gorse I and V wells may be generated from different source rocks. The thermal maturity level of the condensates from the Gorae V well ranges from early to middle oil generation zone and condensate from Gorae I reaches middle oil window. Correlation or the thermal maturation level of the condensates and organic matter in the sediments reveals that a depth of the generation of liquid hydrocarbons can be inferred to 3,000 m and 3,900 m for the Gorae V and I wells, respectively. Gorae V well, however, did not reach the target depth and the geochemical data of the Gorae I well were obscured due to the severe sediment caving in.