• Horticultural Science & Technology
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• v.34 no.2
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• pp.331-341
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• 2016

We analyzed the functional fragrant components of three species of Cymbidium oriental orchids using gas chromatography-mass spectrometry (GC/MS). For the comparative analysis, C. goeringii 'Minchunran', 'Jugeumhwa', C. forrestii 'Chwigae', 'Songmae', 'Yongja', and C. faberi 'Choemae', 'Namyangmae', 'Hwaja' were investigated. Major fragrant components detected by GC/MS were selected on the basis of more than 3% value according to the analysis of peak area (%). We found that ${\alpha}$-bergamotene, which has a cytotoxic effect on breast cancer, cervical cancer, and glioblastoma, and nerolidol, which induces apoptosis of human hepatoma cells (HepG2), inhibits the growth of Streptococcus mutans and babesiosis, and has antibacterial properties, are common substances produced by C. goeringii L. Nerolidol and ${\beta}$-bisabolene, which is cytotoxic and suppresses the growth of malignant melanoma cells (B16-F10), HepG2, and leukemia cells (HL-60, K562), are major substances in C. forrestii R. Furthermore, ${\alpha}$-pinene, which inhibits the growth of gliobastoma cells (SF-767) and inhibits the anti-inflammatory action of hepatoma cells (BEL-7402); 1,8-cineole, which is a potential therapeutic agent for the treatment of gastric ulcers; and 1,3,7-octatriene, which functions as a pheromone, are the most common substances in C. faveri R. Thus, substances identified as major fragrant components in oriental orchid species have multiple beneficial applications in human health. This research forms the basis for further studies of the roles of major fragrant components in oriental orchids.

• Horticultural Science & Technology
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• v.34 no.2
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• pp.324-330
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• 2016

To determine whether FT-IR spectral analysis based on multivariate analysis for whole cell extracts can be used to discriminate between artichoke (Cynara cardunculus var. scolymus L.) plants at the metabolic level, leaves of ten artichoke plants were subjected to Fourier transform infrared(FT-IR) spectroscopy. FT-IR spectral data from leaves were analyzed by principal component analysis (PCA), partial least square discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA). FT-IR spectra confirmed typical spectral differences between the frequency regions of 1,700-1,500, 1,500-1,300 and $1,100-950cm^{-1}$, respectively. These spectral regions reflect the quantitative and qualitative variations of amide I, II from amino acids and proteins ($1,700-1,500cm^{-1}$), phosphodiester groups from nucleic acid and phospholipid ($1,500-1,300cm^{-1}$) and carbohydrate compounds ($1,100-950cm^{-1}$). PCA revealed separate clusters that corresponded to their species relationship. Thus, PCA could be used to distinguish between artichoke species with different metabolite contents. PLS-DA showed similar species classification of artichoke. Furthermore these metabolic discrimination systems could be used for the rapid selection and classification of useful artichoke cultivars.

• Horticultural Science & Technology
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• v.34 no.5
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• pp.719-726
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• 2016

The effect of high and low temperatures on seedling quality while raising of paprika (Capsicum annuum L.) seedlings, and their early development after planting was investigated. The control raising seedling temperature (RST) was $23^{\circ}C$; high temperature, $31^{\circ}C$; and low temperature, $15^{\circ}C$ throughout the raising seedling period. At $15^{\circ}C$ and $30^{\circ}C$, plant height, stem diameter, fresh weight, dry weight, number of leaves, and seedling leaf area were significantly lower than those at $23^{\circ}C$. At 4 weeks after planting, seedling's growth characteristics showed a similar pattern. Compared to $23^{\circ}C$, seedlings raised at $15^{\circ}C$ and $30^{\circ}C$ had an increased dry weight and leaf area per unit time after planting than during the seedling raising period. At 4 weeks after planting, crop growth rate and leaf area index were unaffected by RST, and relative growth rate and net assimilation rate at RSTs of $15^{\circ}C$ and $31^{\circ}C$ were higher than those those at RST of $23^{\circ}C$. At an RST of $15^{\circ}C$, growth speed and net assimilation rates were higher after planting than before planting, according to increased photosynthetic rate. Thus, high and low temperatures during the seedling raising period significantly reduced seedling growth and plant growth after planting. After planting, seedlings raised at $15^{\circ}C$ recovered more quickly than did those raised at $31^{\circ}C$.

• Horticultural Science & Technology
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• v.34 no.5
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• pp.799-806
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• 2016

A standard rose cultivar, 'To Dios'(Rosa hybrida) was selected for use as a cut flower from the progeny of a cross between 'Vanilla perfume' and 'Marcia' at the research and development division of the Goyang-si Agricultural Technology Center in 2013. 'Vanilla perfume', an orange-colored (RHS Orange Group 27C) standard rose cultivar with 48 petals, was used as the female plant. 'Marcia', a white-colored (RHS Green white Group 157B) standard rose cultivar with 96 petals was used as the male parent. A cross was made in 2009 and seedlings were produced. Selections were made between 2010 and 2013, and a plant with good cut flower traits was finally selected and named 'To Dios'. 'To Dios' is a standard rose with large flowers of 11.2 cm in diameter and 128 peach-colored (RHS Red Group 36B) petals per flower. Vase life of this cultivar is up to 15 days. It takes 47 days from pruning to blooming and cut flower productivity is approximately $160stems/m^2$ per year. 'To Dios' was registered as a new cultivar No. 4875 with the Korea Seed & Variety Service on March 19, 2014.

• Horticultural Science & Technology
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• v.34 no.5
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• pp.701-707
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• 2016

The charantin contents of leaves, stems, female and male flowers, and fruits in bitter gourd (Momordica charantia) were analyzed at different harvest seasons to investigate the availability of potential edible parts other than fruits. The charantin contents of fruits ranged from 14.7 to $16.0mg{\cdot}g^{-1}$ dry weight (DW) and those of leaves ranged from 131.4 to $138.0mg{\cdot}g^{-1}DW$, which was eight times higher than in fruits. The charantin contents of female and male flowers and stems were also significantly higher than those of the fruits. The ratio of total charantin content was highest in leaves (48%) of DW, followed by female flowers (20-21%), male flowers (14%), stems (12-13%), and fruits (5%). The charantin contents of fruits harvested 14 days after fruit setting was higher in June to mid-July (20.2 to $23.3mg{\cdot}g^{-1}DW$) than in late July to late August (13.3 to $19.4mg{\cdot}g^{-1}DW$). The higher the fruit weight, the lower the charantin contents, which showed that a negative correlation exists between fruit weight and charantin contents in bitter gourd. These results suggest that to obtain bitter gourd fruits with high charantin contents, fruits should be harvested until mid-July when fruit growth is fast and temperature is high. In addition, leaves, stems, and female and male flowers of bitter gourd can be used as for food, which are known to reduce blood sugar level.

• Horticultural Science & Technology
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• v.34 no.4
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• pp.644-654
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• 2016

Supervised residue trials for mandipropamid in Korean cabbage(Brassica campestris L.) were conducted to establish its pre-harvest residue limit (PHRL), a criterion to ensure the safety of the terminal pesticide residue during cabbage production. Tissues of Korean cabbage were collected at 0, 1, 3, 5, 7, and 10 days after mandipropamid application and subjected to residue analysis. The analytical method was validated by recoveries ranging from 88.2-92.2% at two levels (0.4 and $2.0mg{\cdot}kg^{-1}$), and a limit of quantitation (LOQ) of $0.04mg{\cdot}kg^{-1}$. Mandipropamid residues in Korean cabbage gradually decreased over time. The dissipation rate of the residue would be affected by intrinsic degradation of the compound along with dilution resulting from the fast growth of Korean cabbage. The decay pattern was well fitted by simple first-order kinetics. Biological half-lives of mandipropamid in Korean cabbage ranged from 3.9-4.0 days in two field conditions. Calculated by the regression curve of mandipropamid dissipation, the PHRLs of mandipropamid in Korean cabbage were recommended as 11.07-12.19 and $5.76-6.05mg{\cdot}kg^{-1}$ for 10 and 5 days prior to harvest, respectively.

• Horticultural Science & Technology
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• v.34 no.4
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• pp.587-595
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• 2016

Salt stress in crops in reclaimed tidal lands can be reduced by applying soil amendments. To evaluate the effects of compost, gypsum, and phosphate on the growth of Chinese cabbage in saline-sodic soil conditions, we conducted a pot experiment in 2013 and 2014. The treatments consisted of a standard fertilizer application of a mix of compost and N-P-K fertilizer (S) and standard fertilizer applications with additional compost (S + C), gypsum (S + G), phosphate (S+P), and gypsum and phosphate (S + GP). The mean dry matter yield of cabbage in 2014 was three times as great as that in 2013, although soil EC (Electrical conductivity) in 2014 was not decreased. However, the mean ratio of sodium ion in soil solution ($SAR_{1:5}$) significantly decreased from $17.3{\pm}1.1$ in 2013 to $11.2{\pm}2.7$ in 2014. Application of gypsum had the greatest positive impact on the growth of Chinese cabbage. The S + G treatment increased dry matter yield by 7.0 (48.2) and 7.9 g/plant (16.6%) in 2013 and 2014, respectively, compared to the S treatment. Applying gypsum increased soil EC, but decreased $SAR_{1:5}$ by 14 and 38% in 2013 and 2014, respectively. The application of compost and phosphate had a small effect on the growth of Chinese cabbage. These results suggest that applying gypsum in reclaimed tidal lands can reduce the sodicity of the soil and improve crop growth.

• Horticultural Science & Technology
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• v.34 no.4
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• pp.564-577
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• 2016

Salinity stress limits plant cultivation in many areas worldwide; however, persimmon (Diospyros spp.) has high tolerance to salt. Five accessions of Diospyros [three of Diospyros lotus (accession numbers 824, 846, and 847); one of Diospyros kaki var. sylvestris (869); and one of Diospyros virginiana (844)] were chosen for analysis of salinity stress. We compared the effects of salt stress on plant growth, relative water content (RWC), malondialdehyde (MDA), electrolyte leakage (EL), hydrogen peroxide content ($H_2O_2$), and antioxidative enzyme activities (superoxide dismutase, SOD; catalase, CAT; peroxidase, POD; and ascorbate peroxidase, APX) in leaves of healthy potted seedlings from each of the five accessions after salt treatment for 25 days. Salt stress affected the growth of plants in all five accessions, with all three D. lotus accessions showing the most severe effect. Salt stress increased membrane lipid peroxidation in all accessions, but a stronger increase was observed in the three D. lotus accessions. Moreover, accumulation of $H_2O_2$ was faster in salt-sensitive D. lotus compared to salt-tolerant D. virginiana 844. The activities of all antioxidant enzymes increased in D. virginiana 844 and in D. kaki var. sylvestris 869; the activities of SOD, CAT, and APX were at similar levels in D. virginiana 844 and D. kaki var. sylvestris 869, but POD activity was stimulated to a greater extent in D. virginiana 844. The activities of all antioxidant enzymes (except POD) decreased in D. lotus 824 and increased (except for SOD) in D.lotus 846. The activities of SOD and APX decreased in D. lotus 847, whereas POD and CAT activities both increased. Relative water content decreased significantly in D. lotus. No significant changes in lipid peroxidation or relevant antioxidant parameters were detected in any of the accessions in controls treated with 0.0% NaCl. D. virginiana 844 had higher antioxidant capacity in response to salinity compared to other persimmon rootstocks. These results indicate that changes of these key physiological variables are related to salinity resistance in different accessions of persimmon.

• Horticultural Science & Technology
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• v.35 no.4
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• pp.446-455
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• 2017

We investigated the physicochemical and sensory characteristics of oriental melon (Cucumis melo L.) to provide a consumer-oriented quality index. Oriental melon fruits were harvested at 20, 25, or 30 days after fruit set (DAFS), and each group was sorted by size (small, medium, and large). Fruits harvested at 25 and 30 DAFS had higher CIE $a^*$ and $b^*$ values, higher soluble solids content (SSC), and lower CIE $L^*$, firmness, and titratable acidity (TA) values than fruits harvested at 20 DAFS. Fruits harvested at 25 and 30 DAFS scored more highly for overall acceptance. A significant correlation was found between physicochemical characteristics and overall acceptance. In the delayed-harvest sample, increased sweetness and yellowness, and decreased sensorial texture were associated with an increase in overall acceptance. In principal component analysis, F1 and F2 explained 62.16% and 17.91% of the total variance (80.07%), respectively. Regression analysis of overall acceptance and F1 gave a coefficient of determination ($r^2$) of 0.87. Our results show that consideration of the physicochemical characteristics (CIE value, SSC, pH, SSC/TA ratio, and firmness) and sensory characteristics (yellowness, placenta area condition, oriental melon odor, sweetness, oriental melon flavor, texture, and off odor) of oriental melon in this way can be used as quality indices to predict consumer acceptance.

• Horticultural Science & Technology
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• v.35 no.4
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• pp.393-401
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• 2017

One-year old pear (Pyrus pyrifolia L.) trees of a new commercial cultivar 'Manpungbae', recently developed in South Korea, were planted in 2001 (planting year 1) and trained to four trellis systems: Y-trellis, Y-II-trellis, pergola, and vase-pergola. To evaluate training systems in the local area (southern Korea), tree growth responses were compared for each trellis system from planting years 3 to 8. For trees trained to Y-trellis and pergola systems, a high proportion of land covered by tree canopy was maintained over the study duration, with 70-80% coverage in year 8. Eight-year cumulative yield per tree was increased in the vase-pergola system with a low planting density and an additional scaffold. Compared with other systems, cummulative yield use efficiency was greatest in Y-II-trellis and pergola systems in years 5 and 8. Compared with Y-II-trellis and vase-pergola systems, the 8-year cumulative yield per hectare was two fold greater for trees trained to the Y-trellis (103 tons) and pergola systems (101 tons). Use of the pergola system improved average fruit weight and fruit soluble solid contents, as well as net income in year 8. Given that we observed reduced fruit productivity and increased labor hours (pruning and orchard work), we consider the Y-trellis and pergola systems to be less valuable trellis systems than the others evaluated.