• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.2
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• pp.181-184
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• 2003

We investigated the effect of ethanol on the production of cellulose and acetic acid fermentation by Gluconacetobacter persimmonensis KJ145. Results showed that bacterial cellulose productivity was highest when 2% ethyl alcohol was added to apple-juice medium. For acetic acid production, 7% ethyl alcohol was needed. Optimal concentration of ethyl alcohol was 5% for simultaneous production of bacterial cellulose and acetic acid. For simultaneous production of bacterial cellulose and acetic acid, optimal nitrogen source and optimal concentration were corn steep liquor and 15% (w/v), respectively Optimal culture time for simultaneous production of bacterial cellulose and acetic acid was 14 days. At the optimal condition, Cluconacetobacter persimmonenis KJ145 produced 7.55 g/L of bacterial cellulose (dry weight).

• Journal of the Korean Society for Heat Treatment
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• v.6 no.3
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• pp.138-143
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• 1993

Effects of aging treatment on the microstructural evolution of a rapidly solidified Al-Li-Cu alloy were investigated by differential scanning calorimetry (DSC) and transmission electron microscopy(TEM). It was found that the precipitation sequence was: supersaturated solid solution ${\alpha}^{\prime}$ ${\rightarrow}$ metastable ${\delta}^{\prime}$ + stable precipitate $T_1$ ${\rightarrow}$ stable precipitates ${\delta}+T_1$. Two exothermic and two endothermic peaks are detected by DSC analysis. The two exothermic and endothermic peaks corresponded to ${\delta}^{\prime}$ and ${\delta}+T_1$ precipitation and dissolution reactions respectively. The enthalpy of ${\delta}^{\prime}$ precipitation decreased with increasing of aging temperature and time until the finishing point of precipitation. The activation energy for precipitation of ${\delta}^{\prime}$ was measured as 80KJ/mol and the energy for dissolution was 93KJ/mol. These values arc higher than those of AI-Li binary alloy. Peak hardness value (Hv 170) was obtamed at $210^{\circ}C$ for 1hr aging treatment which coincided with finishing point of ${\delta}^{\prime}$ precipitation.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1993.10a
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• pp.966-978
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• 1993

A power operated 90.5 hp electric motor) grain flour separator was designed and developed for separation of grain (wheat, corn, chickpea and soybean) flour into various fractions based on the size of the particles of the product. The separator agitating mechanism, feed control, cylindrical separator unit and an eccentric mechanism. The machine was tested for wheat ( variety ; Sujata) flour separation into four fractions, viz ; semolina, Gr-I and II, flour (coarse) and white (fine) flour. Wheat samples (6.8% m.c., db) were first pearled by CIAE pearler for 15.8% bran removal . The pearled wheat grains were then milled for semolina by a burre mill. The product and machine characteristics were determined at different capacities varying from 24 kg/h to 143 kg/h. It was found that 76 kg/h capacity gave reasonably best results in terms of purity and recovery of semolina vis-a-vis the market product. The energy requirement of the machine at no-load was found to be 230 W and at load c nditions, it varied between 36.3-6.4 KJ per kg of fead seperation. The macine could be used by small flour millers small/medium size traders and retailers and other processors for making available various flour products of different particle size in the market for ready use of the consumers.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1993.10a
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• pp.979-983
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• 1993

A power operated (0.5 hp electric motor) grain flour separator was designed and developed for separation of grain (wheat, corn, chickpea and soybean) flour it no various fractions based on the size of the particles of the product. The separator is made of mild steel and consists of a hopper, power driven agitating mechanism, feed control , cylindrical separator unit and an eccentric mechanism. The machine was tested for wheat (variety : Subjata) flour separation into four fraction, viz : semolina ; Gr-I and II, flour (coarse) and white (fine) flour. Wheat samples (6.8% m.c., db) were first pearled by CIAE pearler for 15.8% bran removal . The product and machine characteristics were determined at different capacities varying from 24 kg/h to 143 kg/h. It was found that 76 kg/h capacity gave reasonably best results in terms of purity and recovery of semolina vis-a-vis the market product. The energy requirement of the machine at no-load was found to be 230 w and at load conditio s, it varied between 36.3-6.4kj per kg of feed separation. The machine could be used by small flour millers, small/medium size traders and retailers and other processors for making available various flour products of different particle size in the market for ready use fo the consumers.

• Research in Plant Disease
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• v.14 no.3
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• pp.143-152
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• 2008

Mixed-planting of two rice cultivars, HP ('Hopyeongbyeo') and NP ('Nampyeongbyeo'), having a dissimilar susceptibility to rice blast was practiced for chemical-free control of rice blast in the field. The HP/NP combination was selected for applying under mechanized agricultural conditions. Because they have similar genetic characteristics such as seed germination and heading time, culm length, rice quality and size of rice grains except susceptibility to blast. Incidence of panicle blast was reduced 50.4 % compare with supposed blast incidence by HP/NP mixed-planting when the seeds of two cultivars were combined 1 to 1 as weight. Supposed blast incidence was estimated from reduction of rice blast caused by addition of a resistant cultivar NP. Race diversity of Magnaporthe oryzae was examined for correlation with control effect of HP/NP mixed-planting on rice blast. The population of dominant race KJ-101 was diminished and replaced with various co-existing races and eleven new races were appeared in mixed-planting plot. Total number of race isolated from mixed-planting plot was not largely different from mono-culture. However, detection frequency of the new race was increased and variation of the population size of each race was decreased in mixed-planting plots. It was shown that a biased community with a dominant race (KJ-101 or KI-181) was altered to a balanced one of coexisting races. From these results, it was supposed that the balanced diversity among co-existing races within a community might be correlated to control effect by HP/NP mixed-planting on rice blast. Further more, it should be studied that genetic characteristics of the individual race including a virulence on cv. HP and NP was examined for verifying a correlation of mixed-planting effect and race diversity.

• Journal of Aquaculture
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• v.10 no.2
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• pp.143-151
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• 1997

A six week feeding trial was conducted to determine the amount of fish meal analog (FMA) that can be replacing fish meal protein (FM) in Korean Rockfish. Seven experimental diets were formulated on isonitrogenous 52% crude protein and isocaloric basic 16.8KJ/g diet. Also, foreign commercial fish meal analog (CFMA) and attractants (ATT) were tested in this experiment. Percentage of the graded level of replacement of FM by FMA/CFMA on the basis of crude protein were as following : Diet 1, 100%FM ; Diet by 2, 60%FM : 40% CFMA ; Diet 3, 60%FM : 40$ CFMA+ATT ; Diet 4, 80%FM : 20%FMA ; Diet 5, 80%FM : 20% FMA+ATT ; Diet 6, 60%FM : 40%FMA+ATT ; Diet 7, 40%FM : 60% FMA+ATT. The FMA was made by mixing six animal protein source such as th blood meal, squid liver powder, meat and bond meal, leather meal, feather meal, poultry by-product and 3 essential amino acids (Met, Lys, Ile). Weight gain, feed efficiency, specific growth rate and protein efficiency ratio of fish fed diets 4, 5 and 6 were not significantly different (P>0.05) from those of fish fed the control (100% FM), while those of fish fed diets 2 and 3 were significantly lower (P<0.05) than those of fish fed the diet 6. There was no significant ATT effects in this study (P>0.05). Significant differences were found in hepatosomatic index, hemoglobin and condition factor. Therefore, these, results indicated that FMA can be used up to 40% as a substitute of fish meal protein in Korean Rockfish diets.

• Molecules and Cells
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• v.20 no.3
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• pp.385-391
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• 2005

As a first step towards identifying genes involving in the signal transduction pathways mediating rice blast resistance, we isolated 3 mutants lines that showed enhanced susceptibility to rice blast KJ105 (91-033) from a T-DNA insertion library of the japonica rice cultivar, Hwayeong. Since none of the susceptible phenotypes co-segregated with the T-DNA insertion we adapted a map-based cloning strategy to isolate the gene(s) responsible for the enhanced susceptibility of the Hwayeong mutants. A genetic mapping population was produced by crossing the resistant wild type Hwayeong with the susceptible cultivar, Nagdong. Chi-square analysis of the $F_2$ segregating population indicated that resistance in Hwayeong was controlled by a single major gene that we tentatively named Pi-hy. Randomly selected susceptible plants in the $F_2$ population were used to build an initial map of Pi-hy. The SSLP marker RM2265 on chromosome 2 was closely linked to resistance. High resolution mapping using 105 $F_2$ plants revealed that the resistance gene was tightly linked, or identical, to Pib, a resistance gene with a nucleotide binding sequence and leucine-rich repeats (NB-LRR) previously isolated. Sequence analysis of the Pib locus amplified from three susceptible mutants revealed lesions within this gene, demonstrating that the Pi-hy gene is Pib. The Pib mutations in 1D-22-10-13, 1D-54-16-8, and 1C-143-16-1 were, respectively, a missense mutation in the conserved NB domain 3, a nonsense mutation in the 5th LRR, and a nonsense mutation in the C terminus following the LRRs that causes a small deletion of the C terminus. These findings provide evidence that NB domain 3 and the C terminus are required for full activity of the plant R gene. They also suggest that alterations of the resistance gene can cause major differences in pathogen specificity by affecting interactions with an avirulence factor.

• Proceedings of the Mineralogical Society of Korea Conference
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• 2001.06a
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• pp.141-142
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• 2001

남서부 경기육괴의 편마암류로부터 분리된 저어콘(zircon) 입자를 대상으로, 이온현미분석기(ion microprobe)를 사용한 U-Pb 연대를 구하였다. 그 결과는 후기 원생대(약 820 Ma) 뿐만 아니라 오르도비스기에 상당한 화성활동이 한반도에 있었음을 지시한다. 우리 나라 후기 원생대의 화성-변성 활동에 대해 알려져 있는 바는 극히 제한적이어서 후속연구가 필수적이며, 이러한 연구는 한반도의 지체구조적 변천사를 로디니아 초대륙(Rodinia supercontinent)의 생성-분리와 관련해 재조명할 수 있는 기회를 제공할 것이다. 또한 오르도비스기의 화성작용은 그동안 논란이 되어 왔던 소위 “칼레도니아(Caledonian)” 변동 (cf. 조문섭, 2000)에 대한 또 다른 증거를 제공해준다. 저어콘의 연대측정은 서호주의 커튼공업대학교에 설치되어 있는 SHRIMP-II(Sensitive High-Resolution Ion Microprobe-II; 고감도-고분해능 이온현미분석기)를 사용하였으며, 시료 준비 및 분석방법은 기존에 보고된 바와 같다 (e.g., Kinny et al., 1999). 분석된 3개의 암석 시료(1006-5, 8, 9)는 경기육괴의 남서부에 위치한 홍성 지역의 정편마암들이다. 1006-8 시료는 Turek and Kim (1996)이 전통적인 방법을 사용해 687$\pm$5 Ma의 U-Pb 저어콘 연대를 보고한 바 있는 화강암질 편마암 (시료번호, KJ43)에 해당된다. 두 개의 다른 시료는 1006-8 주변에서 산출하는 전형적인 경기육괴의 편마암류로서 화강암질 정편마암이다. 이들 시료로부터 분리된 저어콘 입자들은 대부분 화성기원의 누대구조와 자형의 결정형태를 보여준다. 과성장띠(overgrouth rims)는 1006-5 시료에서 흔하게, 그리고 1006-9 시료에서 매우 드물게 관찰된다. 음극선발광(cathodoluminescence) 영상의 해석을 통해 저어콘 결정의 성장사를 유추하였으며, 이를 바탕으로 이온현미분석 점(spot)을 정하였다. U-Pb-Th 자료는 퍼스(Perth) 저어콘 스탠다드 (CZ3, 564 Ma, $^{206}$Pb/$^{238}$U=0.0914)를 사용하였다. 아래에 기술하는 연대는 모두 $^{206}$Pb/$^{238}$U 연대에 해당된다. 두 개의 화강암질 편마암 시료로부터 구한 U-Pb 저어콘 연대는 각각 812 $\pm$ 14 Ma(1006-8)와 822 $\pm$ 17 Ma(1006-9)로 분석오차 내에서 서로 일치한다. 이 결과는 춘천 및 전곡 지역의 석류석 각섬암에서 보고된 Sm-Nd 전암연대(852 $\pm$ 24 Ma 및 824 $\pm$ 143 Ma; Lee and Cho, 1995; Ree et al., 1996)와 잘 부합한다. 따라서 후기 원생대 기간 중 화성활동이 한반도에서 광범위하게 일어났음을 시사한다. 한편, 1006-9 시료에서는 예외적으로 한 개의 저어콘 입자 주변부(rim)에서 매우 얇은 과성장띠가 관찰되었으며, 두 개의 점 분석으로부터 구한 U-Pb 저어콘 연대는 약 235 Ma이다. 이 띠는 또한 변성기원의 저어콘에서 흔히 관찰되는 작은 W (<0.05) 비를 보인다. 1006-5 시료는 위 두 시료로부터 수 km 떨어진 지점에서 채집하였으나, 저어콘 연대는 상이한 기록을 보여준다. 즉 매우 작은 Th/U (<0.01) 값을 갖는 저어콘의 주변부에서 223 $\pm$ 5 Ma의 연대가 잘 정의되며, 이는 1006-9 시료에서 관찰된 결과와 함께 트라이아스기의 고온변성작용이 백립암상에 가까운, 매우 높은 온도에 달하였음을 지시한다. 한편 저어콘의 중심부는 335-473 Ma의 비교적 넓은 연대 분포를 보인다. 이는 저어콘이 실제 성장한 연대를 지시하기보다는 트라이아스기의 변성작용에 따른 납손실(Pb loss) 그리고 누대 규모보다 더 큰 빔 크기(beam size, 약 30 $\mu\textrm{m}$)의 영향일 것으로 해석된다. 또한 저어콘이 다양한 외래물질로부터 기원했다는 증거가 관찰되지 않으므로, 이 정편마암의 모암은 오르도비스기(약 430-470 Ma)에 관입하였을 것으로 생각된다. 따라서 그동안 논란이 되어 왔던 소위 “칼레도니아” 변동이 한반도 내에 실존하였을 가능성을 시사한다. 이상의 결과를 종합하여 볼 때, 경기육괴의 변성암류는 후기 원생대 이후 다양한 저어콘의 성장사를 기록하고 있음을 알 수 있다: 즉 (1) 후기원생대(약 820 Ma)의 화성작용; (2) 오르도비스기(약 450 Ma)의 화성작용: 그리고 (3) 트라이아스기 (약 223 Ma)의 부분용융을 수반한 고온 변성작용으로 대표된다. 이러한 지질연대는, 옥천변성대에서 얻어진 756 Ma의 저어콘 연대(Lee et al., 1998)와 더불어, 친링-다비-수루(Qinling-Dabie-Sulu) 대륙 충돌대와 양쯔 지괴에서 보고된 지질연대 결과와 잘 부합한다. 따라서 지구연대학적으로 경기육괴가 북중국보다는 대륙충돌대를 포함하는 남중국지괴에 속할 것으로 결론지을 수 있다.