• 제목/요약/키워드: KJ

검색결과 357건 처리시간 0.03초

벼 도열병에 대한 품종의 지속저항성 발현요인에 관한 연구 3. 품종과 도열병균 레이스의 상호관계 (Factors Affecting the Expression of Durable Resistance of Rice Cultivars to Blast Caused by Pyricularia grisea Sacc)

  • 라동수;오정행;한성숙
    • 한국식물병리학회지
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    • 제13권2호
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    • pp.90-94
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    • 1997
  • Interaction of races of rice blast fungus and cultivars has been evaluated. Dongjinbyeo was infected by four KJ races and two KI races, while other varieties were susceptible to four to six KJ or KI races. Among the single spores reisolated from the leaf blast lesion of Dongjinbyeo after mixed inoculation of 10 races, 63% was race KJ-301 which produced a small number and small size lesions, while 30% was race KI-313 which produced a large number of lesions. However, 93% of single spores reisolated from Palgongbyeo was a highly virulent race KJ-105. On the other hand, all the races were equally reisolated from the susceptible cultivar Jinmibyeo. Frequency of races isolated from the naturally infected leaf blast lesions in the field was similar to that of reisolated races from the cultivars inoculated with 10 mixed races in the greenhouse. However, 30% of single spores isolated from the naturally infected Dongjinbyeo was race KI-329 ut ace KI-313 was not detected. Genetic relationship of the isolates collected from leaf and neck blast fungus of Jinmibyeo and Nakdongbyeo showed specific bands on RFLP-P64, However, their genetic similarity was 80%.

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도열병에 감염된 벼의 엽조직에서 Peroxidase의 활성 (Peroxidase Activity in Leaf Tissue of Rice Infected by Pyricularia oryzae)

  • 박원목;이용세;박상호
    • 한국식물병리학회지
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    • 제1권3호
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    • pp.178-183
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    • 1985
  • 수도품종 농백, 진흥, 낙동, 태백 등 4품종과 도열병균 KJ-101과 KJ-301의 2race를 사용하여 peroxidase 활성증가와 저항성과의 관계를 관찰하였다. peroxidase 활성은 수도유균가 성장할수록 모든 품종에서 증가되는 경향은 있었으나 품종간 차이는 크지 않았다. 도열병균을 접종 후 시간이 경과함에 따라 이병 잎에서는 무접종 건전잎보다 효소활성이 높았고 접종된 균주에 비친화적인 품종보다 발병이 심한 친화적 품종에서 더욱 높았다. 전기영동법에 의한 peroxidase pattern은 이병잎에서는 도열병균의 peroxidase band를 관찰할 수 없었다. 질소시비량을 증가시켰을 지라도 peroxidase 활성은 변함이 없었다.

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경제적 에탄올 생산을 위한 균주분리 및 특성 (Isolation and Characterization of a Strain for Economical Ethanol Production)

  • 한효정;김성준
    • KSBB Journal
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    • 제21권4호
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    • pp.267-272
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    • 2006
  • 주정공장 근처 토양에서 분리한 에탄올 발효균주 KJ는 Saccharomyces italicus로 판명되었으며, glucose가 최적의 탄소원으로 확인되었다. 균주 KJ의 최적배양조건은 온도 $30^{\circ}C$, 초기 당 농도 10%, 초기 pH 5 근방, 혐기조건으로 판명되었다. 초기 탄소원이 동일한 조건에서 YM배지(glucose 10 g/L)와 SFW배지(RS 10 g/L)에서 에탄올 생산은 각각 5.34, 5.68 g/L로 나타나, KJ균주는 음식물쓰레기 당화액을 에탄올 발효배지로 적절하게 사용될 수 있음이 확인되었다. 에탄올 생산배양에 SFW의 이용은 에탄올생산단가를 대폭 낮추게 하여 대체에너지인 에탄올의 대량생산기술의 경제성을 확보하는데 크게 기여할 것이다.

An ${\beta}$-1,4-Xylanase with Exo-Enzyme Activity Produced by Paenibacillus xylanilyticus KJ-03 and Its Cloning and Characterization

  • Park, Dong-Ju;Lee, Yong-Suk;Chang, Jie;Fang, Shu-Jun;Choi, Yong-Lark
    • Journal of Microbiology and Biotechnology
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    • 제23권3호
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    • pp.397-404
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    • 2013
  • Paenibacillus xylanilyticus KJ-03 was isolated from soil samples obtained from a field with Amorphophallus konjac plants. A gene encoding xylanase was isolated from KJ-03 and cloned using a fosmid library. The xynA gene encodes xylanase; it consists of 1,035 bp and encodes 345 amino acids. The amino acid sequence deduced from the P. xylanilyticus KJ-03 xylanase showed 81% and 69% identities with those deduced from the P. polymyxa E681 and Paenibacillus sp. HPL-001 xylanases, respectively. The xynA gene comprises a single domain, consisting of a catalytic domain of the glycosyl hydrolase (GH) 10 family. The xynA gene was expressed in Escherichia coli BL21 (trxB), and the recombinant xylanase was purified by Niaffinity chromatography. The purified xylanase showed optimum activity with birchwood xylan as a substrate at $40^{\circ}C$ and pH 7.4. Treatment with $Mg^{2+}$ and $Li^+$ showed a slight decrease in XynA activity; however, treatment with 5 mM $Cu^{2+}$ completely inhibited its activity. The results of the thin layer chromatography analysis indicated that the major hydrolysis product was xylobiose and small amounts of xylose and xylotriose. XynA showed increased activity with oat spelt xylan and birchwood xylan, but showed only slight activity with locust bean gum.

Large-scale Atmospheric Patterns associated with the 2018 Heatwave Prediction in the Korea-Japan Region using GloSea6

  • Jinhee Kang;Semin Yun;Jieun Wie;Sang-Min Lee;Johan Lee;Baek-Jo Kim;Byung-Kwon Moon
    • 한국지구과학회지
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    • 제45권1호
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    • pp.37-47
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    • 2024
  • In the summer of 2018, the Korea-Japan (KJ) region experienced an extremely severe and prolonged heatwave. This study examines the GloSea6 model's prediction performance for the 2018 KJ heatwave event and investigates how its prediction skill is related to large-scale circulation patterns identified by the k-means clustering method. Cluster 1 pattern is characterized by a KJ high-pressure anomaly, Cluster 2 pattern is distinguished by an Eastern European high-pressure anomaly, and Cluster 3 pattern is associated with a Pacific-Japan pattern-like anomaly. By analyzing the spatial correlation coefficients between these three identified circulation patterns and GloSea6 predictions, we assessed the contribution of each circulation pattern to the heatwave lifecycle. Our results show that the Eastern European high-pressure pattern, in particular, plays a significant role in predicting the evolution of the development and peak phases of the 2018 KJ heatwave approximately two weeks in advance. Furthermore, this study suggests that an accurate representation of large-scale atmospheric circulations in upstream regions is a key factor in seasonal forecast models for improving the predictability of extreme weather events, such as the 2018 KJ heatwave.

Bacillus stearothermophilus KJ16이 생산하는 Cyclodextrin Glucanotransferase 의 정제와 효소특성 (Purification and Characterization of Cyclodextrin Glucanotransferase from Bacillus stearothermophilus KJ16)

  • 권현주;남수완;김광현;송승구;윤종원;김병우
    • 생명과학회지
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    • 제8권3호
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    • pp.326-332
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    • 1998
  • Cyclodextrin glucanotransferase from B. stearothermophilus KJ16 that can produce both cyclodextrin glucanotransferase and cyclodextrinase was purified by ammonium sulfate precipitation, DEAE-cellulose chromatography, Sephadex G-100 chromatography, and FPLC. The molecular weight of the purifice enzyme was about 65,000 dalton by SDS-PAGE. The optimal pH and temperature were 6.0 and $60^{\circ}C$, respectively. The enzyme was stable at $50^{\circ}C$ for 1 hr and in the pH range of 5.5 and 8.5. Mercaptoethanol and dithiothreitol inhibited the enzyme activity strongly. The enzyme produced 60% cyclodextrin(CD) from 5% soluble starch with the $^{\alpha}$, $^{\beta}$, $^{\gamma}$-CD ratio of 42:46:12. Amylopectin was the most suitable substrate with 67% conversion to CD.

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Solar Ultraviolet Irradiance Incident on a Horizontal Surface At taegu In Korea During 1995-1998 : (I) Ultraviolet -A

  • Suh, Kye-Hong
    • Journal of Photoscience
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    • 제6권1호
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    • pp.1-3
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    • 1999
  • Solar ultraviolet-A (UV-A) irradiances indicent on a horizontal surface at Taegu of Korea during 1995-1998 were calculated with 5 min averages of measurements taken every 30 seconds by a broadband UV- A sensor. The maximum and minimum of monthly averages of daily UV-A dose were 499.37 KJ m-2 day-1 inJuly and 171.09 KJ m--2 day-1 in December for 4 years of the observation period. The maxima of daily UV-A dose and instandaneous UV-A were observed as 846.46KJ m-2 day-1 June 7, 1998 and 37.22 W m-2 at 12 : 15, July 16, 1998, respectively. Increasing trends in annual maxima of daily UV-A dose and instantaneous UV-A were averaged to 6.4% and 6.7% , respectively, per year during 1995-1998 at Taegu, Korea.

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25KJ 초전도 에너지 저장장치의 전력제어와 발생고조파 분석 (A Power Control of 25KJ Superconducting Magnetic Energy Storage and Analysis of AC Side Current Harmonics)

  • 홍원표;원종수;이승원;황석영
    • 대한전기학회논문지
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    • 제37권11호
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    • pp.762-771
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    • 1988
  • This paper presents the experimental study to confirm the basic properties extract the operating problems of SMES. A new fast control system including u-computer and 12pulse power converter are designed and fabricated. The experiment of 25KJ SMES discharge and charge is carried out by I-C (Inductor-Converter) unit using a new proposed control system. The proposed control algorithm is proved by the simulations and control properties tests. It is also confirmed experimetally that SMES connected I-C unit controlled by this control scheme has an effects on compansating freguency fluctuation by independent active power control.

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경남지방에서 1982~1983년의 벼도열병균레이스 분포 (Races of Pyricularia oryzae in Gyeong-nam Province in 1982~1983)

  • 강수웅;최승락;조동진;유창영
    • 한국응용곤충학회지
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    • 제23권3호
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    • pp.190-192
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    • 1984
  • The 98 and 116 isolates of Pyricularia oryzae collected from Gyeong-nam Province in 1982 and 1983 were differentiated by using Korean differential rice cultivars. Twelve races from98 isolates and 11 races from 116 isolates of P. oryzae were classified indicating that KJ races group was $87\%$ and KI group was $13\%$ in total. The races KJ301 and KJ 401 were ca. $50\%$ in frequency among races identified.

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Cyclomaltodextrinase를 생산하는 Alkalophilic Bacillus sp. KJ-133의 분리와 효소생산 조건 (Isolation of Alkalophilic Bacillus sp. KJ-133 Producing Cyclomaltodextrinase and Its Enzyme Production)

  • 정혜진;권호정
    • 한국미생물·생명공학회지
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    • 제28권4호
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    • pp.219-222
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    • 2000
  • To produce and utilize microbial cyclomaltodextrinase being industrially useful, we isolated an alkalophilic Bacillus strain from soil which was capable of degrading cyclodextrins. The newly isolated strain was aerobic, gram-positive, spore-forming, motile, rod shape(0.2~0.4$\times$1.4~4.4 $\mu\textrm{m}$), and 35.8 mol% of DNA base composition. Based on its morphological, phisiological, and biochemical properties, it was identified as alkalophilic Bacillus sp. KJ-133 and cultivated well in the ranges of $30~40^{\circ}C$ and pH 8.0~9.0 . The cyclomaltodextrinase of the strain showed maximal production after 48h of cultivation at $37^{\circ}C$, and the activity was inhibited by Ag2+, Hg2+, Cu2+, and p-chloromercuribenzoate.

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