• Title/Summary/Keyword: KIT-6

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Effect of High Temperature Steam Oxidation on Yielding of Zircaloy-4 PWR Fuel Cladding -Expanding Copper Mandrel Test- (가압경수형 핵연료 피복관 지르칼로이-4의 항복현상에 대한 고온 수증기 산화의 영향 -구리 맨드렐 팽창시험법-)

  • Kye-Ho Nho;Sun-Pil Choi;Byong-Whi Lee
    • Nuclear Engineering and Technology
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    • v.21 no.2
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    • pp.111-122
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    • 1989
  • With the Zircaloy-4 tube oxidized in high temperature (1323 K) steam for 5, 10, 30 and 60 minutes, the expanding copper mandrel test was carried out over a temperature range of 673-l173k at $\varepsilon\;=\;3.0\times10^5S\;^1$. The oxidation parameters $(K_i)$ in the present study were linearly proportional to square root of time $(Ki= \delta_{kit})$ and their rate constants ($\delta_{ki}$) are 0.281, 2.82, and 2.313 for weight gain and thickness of Zr02 and $\alpha$(0) layer, respectively. Activation energy for high temperature (873-1073k) plastic deformation of Zircaloy-4 increases from 251 KJ/mol to 323 KJ/mol with increase in oxidation time from 5 minutes to 60 minutes due to the high strengthened Zr02. With the oxide layer thickness [K ; expressed in "Equivalent Cladding Reacted" (ECR,%)] and the yield stress obtained from the mandrel test, an empirical relation was derived as ($\sigma/C)^n=K^mexp$ (Q/RT) with n=6.9, m=5.7, C=0.155, 0.138, 0.051, and 0.046 MPa for Q=251, 258, 316, 323 KJ/mol, respectively.

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Prevalence of Canine Giardiasis in the Daejeon and Chungnam Area (대전·충남지역 개에서 지알디아증 유병률)

  • Chung, Dae-Wook;Lee, Sang-Eun;You, Myung-Jo;Seo, Kyoung-Won;Song, Kun-Ho
    • Journal of Veterinary Clinics
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    • v.32 no.6
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    • pp.477-480
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    • 2015
  • A total number of 100 fecal samples were examined for the presence of Giardia intestinalis infection in dogs using a Giardia ELISA kit (SNAP$^{(R)}$ test, IDEXX Laboratories, Inc., Westbrook, ME). 49 males and 51 females were examined for a G. intestinalis infection in Daejeon and Chungnam area of South Korea. The overall positive rate of G. intestinalis infection was 12.00%. G. intestinalis infection resulted to be more prevalent in males (12.24%) than in females (11.76%), and in symptomatic dogs (18.18%) than in asymptomatic dogs (11.54%). There were no significant differences between the two groups. 19.60% being found in the < 2-year-old group, 4.08% in the over 2 year-old group. $X^2$ analysis revealed a significantly higher prevalence (p < 0.05) in the < 2-year-old group than in the other, and a significantly higher prevalence in dogs kept in a shared kennel (36.00%, p < 0.001). This study is the first survey of G. intestinalis infection prevalence in South Korea according to life style (particularly between dogs kept in a shared kennel and that of dogs kept separately) using an ELISA kit, and this study is expected to provide a useful reference for clinicians and breeders.

A Study Regarding Measurements of Bacterial Contamination Levels in Radiology Room Equipment (방사선과 촬영실 장비의 세균오염도 측정)

  • Choi, Eun-Jin;Song, Hyeon-Je;Dong, Kyung-Rae;Kim, Chang-Bok;Ryu, Jae-Kwang;Kwak, Jong-Gil
    • Journal of Radiation Industry
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    • v.11 no.1
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    • pp.1-6
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    • 2017
  • Reported some level of bacteria in areas that are well made contact in Radiology imaging room evaluate the importance of cleanliness in the hospital management of equipment to check for the presence of pathogenic bacteria. Gwang-ju and Jeol-la city and medium-sized hospitals in the material with a cotton swab and rub evenly Radiology selection cassette, a handle, Apron of the imaging apparatus having the most contact with patients from July 2016 to August 2016 as a target in place and special studios 6, and saline solution will placed in a test tube containing. The swab sample was diluted 1,000 times, you can see the bacteria and the intestinal bacterial selective medium Trypticase Soy Agar (TSA), Muller-Hinton Agar (MHA), Eosin-Methylene Blue (EMB), ENDO(BD, NJ, USA) then incubated smear to. In the incubator (incubator, SANYO, Japan) was observed after incubation of bacteria and counting the total number of bacteria also Colonies (colony) suspected intestinal bacteria were isolated and cultured on KIA medium (BD, NJ, USA). As a result, it was found that this came Gram positive Coccus A hospital handle the F hospital, from the C Gram positive Coccus cassette and handle the F hospital. The striking yellow coloring Staphylococcus aureus 110 agar (STA 110) in the medium sample, but it is suspected staphylococcal Coccus to the final identification in the laboratory is not a single specimen of the two samples from Gram positive Coccus biochemical identification Identification Kit is an API could not, it was thought to be non-Staphylococcus aureus was cultured on blood agar suggesting that (BAP) blood of dance. Dynamic tests were conducted biochemical API kit of the two samples were identified from Gram positive Coccus bacteria Escherichia coli (E. coli) is F hospital cassette was confirmed Eenterobacter cloaca in A hospital possession. Did not aggregate O-26, O-111, O-157 and the serum test was conducted in the laboratory from the E. coli F cassette hospital.

Anti-inflammatory Effects of Achyranthes japonica Nakai and Aralia continentalis Kitagawa Complex Fermented Extracts on LPS-stimulated RAW264.7 Macrophage (LPS로 자극된 RAW264.7 대식세포에서 우슬 및 땅두릅 복합 발효추출물의 항염증 효과)

  • Woo, Young Min;Jo, Eun Sol;Kim, Ok Ju;Lee, Young-Ho;Ahn, Mee Young;Lee, Dong-Geun;Lee, Sang-Hyeon;Ha, Jong-Myung;Kim, Andre
    • Applied Chemistry for Engineering
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    • v.30 no.4
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    • pp.479-486
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    • 2019
  • This study investigated the anti-inflammatory effects of mixed extracts of Achyranthes japonica Nakai (Aj) and Aralia continentalis Kitagawa (Ac) (ratios of 1 : 2, 1 : 3, 1 : 5, 2 : 1, 3 : 1 and 5 : 1) on RAW264.7 macrophages. Cell toxicity was determined using a cell counting kit (CCK) assay. We evaluated anti-inflammatory effects of the mixed extracts of Aj and Ac by measuring interleukin $(IL)-1{\beta}$, IL-6, and tumor necrosis factor $(TNF){\alpha}$ using an enzyme-linked immunosorbent assay (ELISA) kit assay. The mixed extracts of Aj and Ac inhibited lipopolysaccharide (LPS)-induced $IL-1{\beta}$ and $TNF{\alpha}$ in LPS-stimulated macrophages. Comparing different ratios of the mixed extracts, the 2 : 1 ratio of Aj and Ac has much more potency and inhibited the production of $TNF{\alpha}$ in LPS-induced RAW264.7 cells. The results of the present study showed that the mixed extracts of Aj and Ac have potential anti-inflammatory effects on RAW264.7 macrophages. Therefore, these extracts may be used as a good source of functional foods for the protection against inflammatory diseases.

Usability of CPR Training System based on Extended Reality (확장현실 기반의 심폐소생술 교육 시스템의 사용성 평가)

  • Lee, Youngho;Kim, Sun Kyung;Choi, Jongmyung;Park, Gun Woo;Go, Younghye
    • Journal of Internet of Things and Convergence
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    • v.8 no.6
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    • pp.115-122
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    • 2022
  • Recently, the importance of CPR training for the layperson has been emphasized to improve the survival rate of out-of-hospital cardiac arrest patients. An accurate and realistic training strategy is required for the CPR training effect for laypersons. In this study, we develop an extended reality (XR) based CPR training system and evaluate its usability. The XR based CPR training system consisted of three applications. First, a 3D heart anatomy image registered to the manikin is transmitted to the smart glasses to guide the chest compression point. The second application provides visual and auditory information about the CPR process through smart glasses. At the same time, the smartwatch sends a vibration notification to guide the compression rate. The 'Add-on-kit' is a device that detects the depth and speed of chest compression via sensors installed on the manikin and sends immediate feedback to the smartphone. One hundred laypersons who participated in this study agreed that the XR based CPR training system has realism and effectiveness. XR based registration technology will contribute to improving the efficiency of CPR training by enhancing realism, immersion, and self-directed learning.

Investigation of microbial contamination in meal kit products purchased via online shopping (온라인에서 구매한 밀키트 제품의 미생물 오염도 조사)

  • Lee, Seung Hun;Nam, Su Jin;Kim, Dong Woo;Kim, Gi Ryeon;Park, Seon Jeong;Lee, Eun Ji;Je, Hyeon Ji;Koo, Ok Kyung
    • Korean Journal of Food Science and Technology
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    • v.54 no.2
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    • pp.235-240
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    • 2022
  • In this study, the delivery conditions and microbial contamination in Mille-feuille Nabe and fresh spring roll meal kits purchased online were assessed. The average surface temperature on the product arrival was 14.4℃. The average number of total aerobic bacteria and coliforms in Mille-feuille Nabe was 3.27 log CFU/g and 1.27 log CFU/g, respectively, in meat, and 6.66 log CFU/g and 2.94 log CFU/g, respectively, in vegetables. The average number of total aerobic bacteria and coliforms in fresh spring rolls was 3.82 log CFU/g and 1.93 log CFU/g in meat, and 5.62 log CFU/g and 3.31 log CFU/g in vegetables, respectively. Escherichia coli was detected in bok choy and perilla leaves, with an average of 0.86 log CFU/g. Salmonella spp. and E. coli O157:H7 were not detected in any of the samples; however, Listeria monocytogenes was detected in all three beef samples. Therefore, before consuming meal kits, sufficient washing and heating are recommended to prevent occurrences of food poisoning.

A Study on Precise Tide Prediction at the Nakdong River Estuary using Long-term Tidal Observation Data (장기조석관측 자료를 이용한 낙동강 하구 정밀조위 예측 연구)

  • Park, Byeong-Woo;Kim, Tae-Woo;Kang, Du Kee;Seo, Yongjae;Shin, Hyun-Suk
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.28 no.6
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    • pp.874-881
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    • 2022
  • Until 2016, before discussions on the restoration of brackish water of the Nakdong River Estuary started in earnest, the downstream water level was predicted using the data of existing tide level observatories (Busan and Gadeokdo) several kilometers away from the estuary. However, it was not easy to carry out the prediction due to the dif erence in tide level and phase. Therefore, this study was conducted to estimate tide prediction more accurately through tidal harmonic analysis using the measured water level affected by the tides in the offshore waters adjacent to the Nakdong River Estuary. As a research method, the storage status of observation data according to the period and abnormal data were checked at 10-minute intervals in the offshore sea area near the Nakdong River Estuary bank, and the observed and predicted tides were measured using TASK2000 (Tidal Analysis Software Kit) Package, a tidal harmonic analysis program. Regression analysis based on one-to-one comparison showed that the correlation between the two components was high correlation coef icient 0.9334. In predicting the tides for the current year, if possible, more accurate data can be obtained by harmonically analyzing one-year tide observation data from the previous year and performing tide prediction using the obtained harmonic constant. Based on this method, the predicted tide for 2022 was generated and it is being used in the calculation of seawater inflow for the restoration of brackish water of the Nakdong River Estuary.

Effect of Polydeoxyribonucleotide on Human Periodontal Ligament Cells as a Storage Medium for Avulsed Tooth (탈구치 저장 매체로서 치주인대 세포에 미치는 Polydeoxyribonucleotide의 효과에 대한 연구)

  • Sang Tae Ro;Yong Kwon Chae;Ko Eun Lee;Mi Sun Kim;Ok Hyung Nam;Hyoseol Lee;Sung Chul Choi
    • Journal of the korean academy of Pediatric Dentistry
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    • v.50 no.3
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    • pp.347-359
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    • 2023
  • Objective: This study aimed to evaluate the suitability of polydeoxyribonucleotides (PDRN) as a storage medium for avulsed teeth. Materials and Methods: The viability of human periodontal ligament (PDL) cells stored in Hank's balanced salt solution and PDRN solutions (concentrations, 10, 25, 50, and 100 ㎍/mL) and tap water was measured using the Cell Counting Kit-8 and Live/Dead assays. In addition, Nitric oxide detection and quantitative real-time polymerase chain reaction (qRT-PCR) were performed to evaluate the anti-inflammatory effect of PDRN. Results: The viability of PDL cells stored in a 100 ㎍/mL PDRN solution was significantly higher than that of cells stored in the other solutions (p < 0.01). Furthermore, cells stored in 100 ㎍/mL PDRN solution demonstrated a significantly reduced NO production (p < 0.0001), and cells stored in 50 and 100 ㎍/mL PDRN solutions expressed significantly lower levels of tumor necrosis factor α, interleukin (IL) -4, IL-6, and IL-10 (p < 0.01) compared to cells stored in HBSS. Conclusion: The PDRN solution exhibited cell-preserving and anti-inflammatory effects on the PDL cells. The findings of this study can serve as a basis for further experiments directed at the development of an effective storage medium for avulsed teeth.

Fruit Morphology, Citrulline, and Arginine Levels in Diverse Watermelon (Citrullus lanatus) Germplasm Collections

  • Awraris Derbie Assefa;On-Sook Hur;Na-Young Ro;Jae-Eun Lee;Ae-Jin Hwang;Bit-Sam Kim;Ju-hee Rhee;Jung Yoon Yi;Ji Hyun Kim;Ho-Sun Lee;Jung-Sook Sung;Myung-Kon Kim;Jae-Jong Noh
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.08a
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    • pp.33-33
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    • 2020
  • Watermelon (Citrullus lanatus) is a non-seasonal, economically important, cucurbit cultivated throughout the world with Asia as a continent contributing the most. As part of the effort in diversifying watermelon genetic resources in the already cultivated group, this study was devoted to providing baseline data on morphological quality traits and health-beneficial phytonutrients of watermelon germplasm collections, thereby promoting watermelon research and cultivation programs. To this end, we reported morphological traits, citrulline, and arginine levels of watermelon genetic resources obtained from the gene bank of Agrobiodiversity Center, Republic of Korea, and discussed the relationship between each other. Diverse characteristics were observed among many of the traits. But, most of the genetic resources (>90%) were either red or pink-fleshed. Korean origin fruits contained intermediate levels of soluble solid content (SSC) while The USA, Russian, Tajikistan, Turkmenistan, Taiwan, and Uruguay originated had generally the highest levels of soluble solids. The citrulline and arginine contents using HPLC method were ranged from 6.9 to 52.1 mg/g (average, 27.3 mg/g) and 1.8 to 21.3 mg/g (average, 9.8 mg/g), respectively. The citrulline content determined using Citrulline Assay Kit was ranged from 6.5 to 42.8 mg/g (average, 27.0 mg/g). Resources with high citrulline and arginine levels contained low SSC. Whereas, red- and pink-colored flesh samples had less citrulline compared to yellow and orange. In addition to the profiling of morphological characters and phytonutrients, molecular marker characterization and identification of sources of resistance to diseases and pests are recommended for a more complete diversity analysis of watermelon genetic resources.

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Afatinib Reduces STAT6 Signaling of Host ARPE-19 Cells Infected with Toxoplasma gondii

  • Yang, Zhaoshou;Ahn, Hye-Jin;Park, Young-Hoon;Nam, Ho-Woo
    • Parasites, Hosts and Diseases
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    • v.54 no.1
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    • pp.31-38
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    • 2016
  • Specific gene expressions of host cells by spontaneous STAT6 phosphorylation are major strategy for the survival of intracellular Toxoplasma gondii against parasiticidal events through STAT1 phosphorylation by infection provoked $IFN-{\gamma}$. We determined the effects of small molecules of tyrosine kinase inhibitors (TKIs) on the growth of T. gondii and on the relationship with STAT1 and STAT6 phosphorylation in ARPE-19 cells. We counted the number of T. gondii RH tachyzoites per parasitophorous vacuolar membrane (PVM) after treatment with TKIs at 12-hr intervals for 72 hr. The change of STAT6 phosphorylation was assessed via western blot and immunofluorescence assay. Among the tested TKIs, Afatinib (pan ErbB/EGFR inhibitor, $5{\mu}M$) inhibited 98.0% of the growth of T. gondii, which was comparable to pyrimethamine ($5{\mu}M$) at 96.9% and followed by Erlotinib (ErbB1/EGFR inhibitor, $20{\mu}M$) at 33.8% and Sunitinib (PDGFR or c-Kit inhibitor, $10{\mu}M$) at 21.3%. In the early stage of the infection (2, 4, and 8 hr after T. gondii challenge), Afatinib inhibited the phosphorylation of STAT6 in western blot and immunofluorescence assay. Both JAK1 and JAK3, the upper hierarchical kinases of cytokine signaling, were strongly phosphorylated at 2 hr and then disappeared entirely after 4 hr. Some TKIs, especially the EGFR inhibitors, might play an important role in the inhibition of intracellular replication of T. gondii through the inhibition of the direct phosphorylation of STAT6 by T. gondii.