• Tuberculosis and Respiratory Diseases
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• v.50 no.2
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• pp.205-212
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• 2001

Background : The information on nasal transport and the metabolism of peptides have been obtained from pharmacokinetic investigations in experimental animals. However, there are no transport and metabolic studies of human nasal epithelial cells. In this study, the permeability characteristics and the metabolic properties of in vitro human nasal cell monolayers were investigated. Material and Methods : Normal human inferior nasal conchal tissue samples were obtained from patients undergoing endoscopic nasal cavitary surgery. The specimens were cultured in a transwell using an air-liquid Interface (ALI) culture, and the transepithelial electrical resistance (TEER) value of the blank filter and confluent cell monolayers were measured. To determine the % leakage of mannitol, $4{\mu}mol%$ $^{14}C$-labelled mannitol was added and the % leakage was measured every 10 minute for 1 hour. Result : Human nasal epithelial cells in the primary culture grew to a confluent monolayer within 7 days and expressed microvilli. The tight junction between the cells was confirmed by transmission electron microscopy. The TEER value of the blank filter, fifth day and seventh day reached $108.5\;ohm.cm^2$, $141\;ohm.cm^2$ and $177.5\;ohm.cm^2$, respectively. Transcellular % leakage of the $^{14}$-mannitol at 10, 20, 30, 40, 50 and 60 minutes was $35.67{\pm}5.43$, $34.42{\pm}5.60$, $32.75{\pm}5.71$, $31.76{\pm}4.22$, $30.96{\pm}3.49$ and $29.60{\pm}3.68\;%$, respectively. Conclusion : The human nasal epithelial monolayer using ALI culture techniques is suitable for a transcellular permeability study. The data suggests that human nasal epithelial cells In an ALI culture technique shows some promise for a nasal transport and metabolism study.

• Tuberculosis and Respiratory Diseases
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• v.50 no.4
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• pp.409-414
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• 2001

Background : Legionella pneumophila has been recognized as an important cause of community-acquired pneumonia(CAP) requiring hospitalization. However, epidemiological data on the occurrence of legionella-related pneumonia is unavailable in Korea. The purpose of this study was to evaluate the etiological importance of legionella pneumophila serogroup 1 in patients hospitalized with CAP. Method : The CAP patients over 16 year-old were recruited from July 1999 to June 2000 at the Chunchon Sacred Heart Hospital. Fifty four patients (male 29, female 25, average age $63.8{\pm}15.3$) were included in this study. A diagnosis of a legionella pneumophila infection was based on a urinary antigen test using the Binax Company enzyme immunoassay. The severity of pneumonia was assessed using the Fine's PORT scoring system. Result : The average Fine's PORT score was 99.7(${\pm}44.9$). According to the risk classification proposed by the Infectious Disease Society of America, the number of patients in each class(from class I to class V) were 6(11.1%), 13(24.1%), 9(16.7%), 14(25.8%), and 12(22.2%), respectively. Thirty two patients(59.3%) were initially admitted to the intensive care unit. The mortality rate was 16.7%(9 in 54). In all patients, urinary antigens to Legionella pnewnophila serogroup 1 were not detected. Conclusion : Legionella pnewnophila may play little role in causing adult CAP in Korea. Therefore, the routine use of macrolide in the empirical treatment of the CAP patients based upon the ATS guidelines(1993) in Korea should be reevaluated.

• Tuberculosis and Respiratory Diseases
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• v.64 no.6
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• pp.427-432
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• 2008

Background: A paradoxical response is defined as the radiological and clinical worsening of a previous lesion or the development of new lesion after initial improvement during theprocess of antituberculous treatment. The related factors for the development of a paradoxical response in patients with tuberculous pleurisy are not certain. Methods: We selected patients with tuberculous pleurisy who had been treated for more than 4 months. The changes onthe serial chest X-ray findings before and after treatment were reviewed. Paradoxical responses were regarded as any worsening or development of new lesion at least 2 weeks after the initiation of treatment. The baseline clinical characteristics and laboratory findings of the peripheral blood and pleural fluid were compared between the patients with a paradoxical response and the patients without a paradoxical response. Results: Paradoxical responses appeared in sixteen patients (21%) among the 77 patients.It took a mean of 38.6 days after the treatment and the time to resolve the paradoxical response was a mean of 32.1 days. For the patients with a paradoxical response, the median age was younger (30.5 years vs 39.0 years, respectively) and the lymphocytic percentage of white blood cells in the pleural fluid was higher (82.1% vs 69.6%, respectively) than for the patients without a paradoxical response. Conclusion: The development of a paradoxical response during the treatment of patients with tuberculous pleurisy was not rare and this was related with the age of the patients and the percentage of lymphocytic white blood cells in the pleural fluid.

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.1
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• pp.30-38
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• 2018

This study was conducted to explore the effects of seeding rate of a seed mixture on grassland productivity and botanical composition. Seeding rates were composed of four different treatments varying by the amount of seed: T1, 50% decrease in seeding rate from standard; T2, standard seeding rate; T3, 50% increase in seeding rate from standard; and T4, 100% increase in seeding rate from standard. In 2016, seasonal changes in grass botanical composition revealed T2, T3, and T4 in portion of grass ratio were the highest with a range of 87-100%, whereas T1 was the lowest of all treatments. In 2017, the botanical composition in T1 showed that the grass ratio in of the first cutting was 91%, but in the fourth cutting its ratio decreased by 75%. The results from both years were combined for each treatment, T4 ($11,435kg\;ha^{-1}$) and T3 ($11,162kg\;ha^{-1}$) demonstrated the highest dry matter yield of the treatments (T1: $8,196kg\;ha^{-1}$; T2: $9,521kg\;ha^{-1}$) (p<0.05). As a result, a 50-100% increase in the seeding rate from the standard demonstrated the best grassland yield and botanical composition.

• Korean Journal of Environmental Agriculture
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• v.26 no.2
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• pp.107-115
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• 2007

Objective of this study was to assess the efficient rice cultivation practice to mitigate the non-point source pollutants loading to the adjacent watershed. Cultivation practices consisted of machine transplanting, direct seeding on dry paddy, and no tillage in which no fertilizer and pesticide were applied to paddy field. Water in drainage canal was used as irrigation source during the entire rice growing season. Loading of the non-point source pollutants to the adjacent small stream was mitigated by all treatments. Rice yield, total biomass (rice + weeds), and uptake T-N, $P_2O_5$, and $K_2O$ were higher in machine transplanting practice than those in direct seeding and no tillage practices. However, the purification effects of non-point source pollutants were followed in orders of no tillage > direct seeding > machine transplanting due to quantity of irrigation water. The annual purification quantity of T-N, T-P, and K by rice cultivations ranged from 46 to 369 kg $ha^{-1}$, 4.1 to 16.4 kg $ha^{-1}$, and 55 to 238 kg $ha^{-1}$, respectively, during the entire rice growing season. Results revealed that no tillage practice of rice cultivation was the best management option in reducing the loading of the non-point source pollutants from the drainage canal into stream.

• Korean Journal of Environmental Agriculture
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• v.26 no.3
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• pp.246-253
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• 2007

This study was performed to enhance the cleanup efficiency of methoxyfenozide and bentazone by pH adjustment in the course of liquid-liquid partition and to develop an optimum analytical conditions using HPLC coupled with DAD for two matrices, brown rice and rice straw. Preparation procedure of brown rice sample was "extraction${\rightarrow}$coagulation${\rightarrow}$liquid-liquid partition$\rightarrow$-florisil C.C", and this procedure was samely applied to two compounds. In rice straw, preparation procedure of methoxyfenozide sample was "extraction$\rightarrow$-alkalization$\rightarrow$liquid-liquid extraction$\rightarrow$coagulation$\rightarrow$florisil C.C", and in the case of bentazone, "extraction$\rightarrow$alkalization$\rightarrow$liquid-liquid partition$\rightarrow$acidification$\rightarrow$liquid-liquid extraction$\rightarrow$florisil C.C". All these purified samples were redissolved in the mobile phases, acetonitile : 20 mM sodium acetate (75:25, v/v) for methoxyfenozide and acetonitrile : 75 mM sodium acetate, pH 6.0 (40:60, v/v) for bentazone. Recoveries of methoxyfenozide analysis in brown rice and rice straw were 83.5-97.4 and 86.4-97.3%, and detection limits were 0.02 and 0.04 mg/kg, respectively. Recoveries of bentazone in brown rice and rice straw were 86.8-101.9 and 88.3-94.5% and detection limits were 0.005 and 0.01 mg/kg, respectively. This methods seem to be usefully applied to the residue analysis of two compounds in the view of producing stable analytical condition and fair reproducibility.

• Korean Journal of Plant Resources
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• v.31 no.2
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• pp.124-135
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• 2018

The study was performed to explore the molecular changes in the vegetative stage (3-and 5-leaf) of sorghum under waterlogging stress. A total of 74 differentially expressed protein spots were analyzed using LTQ-FT-ICR MS. Among them, 12 proteins were up-regulated and 3 proteins were down-regulated. Mass spectrometry (MS) results showed that about 50% of the proteins involved in various metabolic processes. The level of protein expression of malate dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase related to carbohydrate metabolic process increased in both 3 and 5-leaf stage under waterlogging stress. These proteins are known to function as antistress agents against waterlogging stress. The expression of oxygen-evolving enhancer protein 1 protein related to photosynthesis was slightly increased in the treated group than in the control group, however the expression level was increased in the 5-leaf stage compared to the 3-leaf stage. Probable phospholipid hydroperoxide glutathione peroxidase protein and superoxide dismutase protein related to response to oxidative stress showed the highest expression level in 5-leaf stage treatment. This suggests that the production of reactive oxygen species by the waterlogging stress was the most abundant in the 5-leaf treatment group, and the expression of the antioxidant defense protein was increased.

• Journal of dental hygiene science
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• v.7 no.3
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• pp.141-146
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• 2007

A questionnaire survey of patients for practical training of dental hygiene department of E college was conducted from August 28th 2006 to December 8th 2006. Before scaling, the purpose of the study and the questionnaires were fully explained and the patients were requested to complete the questions personally. The survey were conducted under their consent and 256 copies were collected and used for the analysis. The survey shows the patients' habit for oral health care according to the gender and age and their standards for the quality of life. 1. For gender, male occupied 117(45.7%) and female occupied 139(54.3%). For age, 158 of the patients were in their twenties(61.7%) and 60 of them were aged from 10 to 19(23,4%). 16 patients were aged from 40 to 49(6.3%) and 11 patients were aged from 30 to 39(4.3%) and the number of patients who are older than 50 was 11(4.3%). 2. For the mouth health care, according to gender, 37.6% of male answered that they had scaling and 46.8% of female patients answered that they had scaling experiences. Without distinction of gender, most patients answered they brushed their teeth twice a day. For 91.5% of male patients and 89.9% of female patients answered that they brushed their teeth after meals. Also, 41% of male patients and 53.2% of female patients use rolling method. For using mouth aids, 68.4% of male patients and 65.5% of female patients do not use any aids. 3. For brushing way, most teenagers and patients in their twenties use rolling method and patients older than thirty use vertical and horizontal method. The frequency of brushing shows that most of the respondents brush their teeth twice a day after meals. 4. The standard of quality of life according to gender, under 'clear pronunciation' head, male patients mark 4.42 points and female patients mark 4.17 points. Under 'every day going out' head, male patients mark 4.53 points and female patients mark 4.29 points. Under 'maintenance of emotion' male patients mark 4.27 points and female patients mark 4.27 points. 5. The standard of quality of life according to age, teenage patients show 4.28 points under 'having meals' and under 'clear pronunciation' head, patients in their forties mark 4.44 points. Under 'keeping teeth or denture clean' head, patients in their thirties mark 4.55 points. Patients in their fifties show the lowest grades in most questionnaires.

• Journal of dental hygiene science
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• v.13 no.4
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• pp.493-500
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• 2013

The study aims to determine the status of oral health of mental illness patients and establish the preliminary data. The examinations and questionnaire survey were done 92 psychiatric patients to measure sociodemographic characteristics, decayed, missing and filled teeth (DMFT) index, patient hygiene performance (PHP) index, community periodontal index of treatment need (CPITN). Result of the missing teeth index in the state of oral health was higher in the hospital group (6.42) while the filled teeth index was higher in the center group (4.78). In the DMFT index, mental illness patients were higher than the national sample. The oral health status of medical aid recipients was poorer as the subjects were older and less educated (p<0.05). The PHP index was 3.41, close to the bad oral hygiene state. The hospital group (81.7%) required higher need for periodontal treatment. The periodontal health state was much poorer especially when the subject was in the age of 40's and 60's, received less education, and had no family (p<0.05). $CPITN_3$ was higher in the hospital group (13.3%) than the national sample (5.7%). The mental illness patients were socially vulnerable, therefore oral health care program should be needed and age, education level, health insurance type, presence of family and other factors needs to be considered in this approach.

• Korean Journal of Environment and Ecology
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• v.23 no.1
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• pp.41-49
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• 2009

This research conducted a survey on the feeding crane's size, formation and distribution distance subsequent to road pattern and feeding flock's size during the wintering season eight times at the Cheolwon basin in Korea from December 2005 until February 2006. The survey results are as follows: First, the feeding flocks of the Red-crowned and White-naped Crane were found to mainly consist of two to four individuals. Mono-specific feeding flocks were more identified than hetero-specific feeding flocks in both Red-crowned and White-naped Cranes. There existed a significant difference in the ratio by size between the feeding Red-crowned and White-naped Cranes. The crane's average distribution distance from the paved road was much farther than from the unpaved road, paved farm road and unpaved farm road. In case of the white-naped crane, there was a difference in its distance length from between the road and farm road and there existed a significant difference only in the paved road in the distance length by road pattern between these two bird species[Red-crowned and White-naped Crane]. Such a result indicates that crane species don't prefer the road with frequent vehicular traffic to the road with low vehicular traffic. 5 or more individuals of feeding flocks of the Red-crowned Crane were located at a farther distance length from the paved road comparing to less than 5 individuals of the whole feeding flocks and less than 5 individual feeding flocks. 5 or more individuals of feeding flocks of the White-naped Crane were located at a farther distance length from the paved road, paved farm road and unpaved farm road than less than 5 individuals of feeding flocks of the White-naped Crane; however, there existed no difference in distance length subsequent to their group size. Conclusively, it was found out that the flocks with a larger size were more greatly affected by interrupting factors. Considering such results, there seems to be a more relation to the fact that larger size feeding flocks of crane species have a higher efficiency in the lookout for their surroundings.