• Korean Journal of Microbiology
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• v.32 no.1
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• pp.12-19
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• 1994

KEM1 is known to control the spindle pole body or microtubule function, probably in response to the cellular nutritional conditions in Saccharomyces cerevisiae. Transposon insertions were performed in the cloned KEM1 gene using mini-Tn10-LUK element carrying E. coli ${\beta}$-galactosidase structural gene. A collection of ranfom Tn10-LUK insertions defined an approximately 3.5 kb region required for the KEM1 function. From this collection functional KEM1::lacZ protein fusions were identified. Indirect immunofluorescence using anti-${\beta}$-galacatosidase antibodies localized the KEM1::lacZ fusion protein to the periphery of the nucleus.

• Korean Journal of Microbiology
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• v.30 no.1
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• pp.37-41
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• 1992

The KEM1 gene is known to affect microtubule and spindle pole body function during the cell division cycle in Saccharomjyces cerevisiae. To identify new genes with functions similar or related to those of KEM1, we isolated a high copy suppressor gene (ROK1) that suppresses the kem1 mutation when cloned on a high copy number plasmid but not on a low copy number plasmid. Two clones which suppress both the benomyl hypersensitivity and the $Kar^{-}$ enhancing phenotype of kem1 null mutation were isolated and were shown to have a 9.0 kb identical insert by restriction endonuclease analysis. The restriction map constructed indicates that this suppressor gene, ROK1 is not KEM1. Subcloning experiments suggest that the functional region of ROK1 is at least 3.0kb in size.

• Korean Journal of Microbiology
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• v.54 no.1
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• pp.81-83
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• 2018

We sequenced the genome of the Neisseria sp. KEM232 isolated from the smooth surface caries of human cavity of a 7-year old male in Republic of Korea by using the standard dilution plating technique. The genome comprises a single circular 2,371,912 bp chromosome with a G + C content of 58.5%, 2,210 protein-coding genes, 108 pseudo genes, 51 RNA genes, and one CRISPR array. Based on the 16S rRNA gene sequence similarity and average nucleotide identity, the strain KEM232 is most closely related to Neisseria baciliformis.

• 대한공업교육학회지
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• v.30 no.1
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• pp.133-148
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• 2005

This research attempts to establish a strategy through which to secure the introduction of the KEM 2.0 system. Another aim of this research is to eventually establish a service system which can be used to automatically generate and provide the metadata information contained in the Cylearn system. in order to facilitate Korean vocational high school students access to teaching-learning materials. The main research tasks associated with this research were: 1) To establish the components of the file server system while taking into consideration the environment in which each educational organization operates; 2) To utilize the KEM 2.0 in order to optimize the configuration of the Cylearn system and the related software. To do so, the KEM 2.0. should be applied in close coordination with Edunet: The results of this research can be summarized as follows: First, a strategy to introduce the KEM 2.0 was established. To achieve this, the researcher analyzed the characteristics of the sequencing and presentation methods and suggested teaching-learning materials based on the KEM 2.0.s sequencing system. Second, the file server was constructed using the KEM 2.0. The established file server took into account the environmental conditions in which the Edunet system operates so as to facilitate the creation of a network system with Edunet. Third, the Cylearn service system was linked to Edunet. The researcher developed a module using the KEM 2.0. that could be used to transmit the metadata related to teaching-learning materials to Edunet. To achieve this, an input device and a databank which could be used to transfer the generated metadata to Edunet were developed. The input device and databank developed using the KEM 2.0. were used to transfer the metadata to Edunet by linking the Cylearn system to the Edunet system.

• Proceedings of the Korea Information Processing Society Conference
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• 2005.05a
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• pp.509-512
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• 2005

인터넷과 웹과 같은 정보사회의 교육환경 변화에 따른 교육정보의 교류 수요의 급속한 증가에 따라 교육정보 자료에 대한 메타데이터의 표준이 필요하게 되었다. 이에 따라 국내에서는 개별적으로 개발되고 있는 교육정보 자료를 통합적으로 공유, 재사용하기 위하여 KEM(Korea Education Metadata) Ver 2.0을 한국교육학술정보원에서 개발하였다. 또한 해외에서는 최근 급증하고 있는 멀티미디어 데이터의 적절한 표현을 위하여 MPEG-7 표준이 제정되었다. 본 논문에서는 다양한 형태의 교육정보 자료 중에서 교육정보 이미지에 대한 검색시스템을 설계하였다. 본 검색시스템의 설계는 한국교육학술정보원에서 개발된 KEM Ver 2.0을 수용하기 위하여 교육정보 이미지의 메타데이터 표현을 KEM Ver 2.0에서 제안된 메타 데이터 표현을 MEPG-7으로 확장한 XML 스키마를 사용하였다. KEM Ver 2.0 기반의 high-level 메타데이터와 MEPG-7 기반의 low-level 메타데이터를 이용함에 따라 국내에서 사용되는 교육정보 이미지의 상호교환 뿐 아니라 체계적이고 빠른 검색과 추출이 가능할 것이다.

• The Journal of Korean Association of Computer Education
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• v.12 no.3
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• pp.75-83
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• 2009

Moving picture has become the important media in education with expanded e-learning paradigm, but Korea Educational Metadata has limitation about representing information of lots of events and objects in moving picture. Announcing the MPEG-7 specification the information of lots of events and objects in it can be presented in terms of semantic and structural description of moving pictures. In this paper moving picture searching system model that integrates two metadata specifications, such as KEM and MPEG-7, is proposed. In this model one ontology to combine two metadata specifications is designed, and the other ontology about knowledge of a subject matter is added to search efficiently in searching system. As some moving picture data from Edunet were selected and stored in our server, our prototype of searching system using MPEG-7 and KEM shows the results that we are expected.

• Journal of the Korea Society of Computer and Information
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• v.10 no.4 s.36
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• pp.155-164
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• 2005

WThe education information have been increased. Accordingly, the necessary of developing on education information metadata standards has been increased. By the reason, the Korea Education & Research Information Service developed KEM(Korea Educational Metadata) 2.0. And MPEG-7 was developed to describe metadata of multimedia data. In this paper, we developed a education information image retrieval system. This system used XML schema to accept education information image metadata. We integrated contents-based retrieval and a semantic-based retrieval to overcome there problems that content-based retrieval system can not support semantic-based retrieval and a semantic-based retrieval can not support content-based retrieval. As a results, we expect to handle metadata more efficiently.

• The Microorganisms and Industry
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• v.14 no.3
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• pp.3-6
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• 1988

본고에서는 kem1 유전자의 기능에 대해 중점적으로 연구한 결과를 서술하려 한다.

• Journal of Digital Contents Society
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• v.7 no.2
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• pp.125-131
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• 2006

In the recent year, the need for effective searching for e-learning contents has emerged as a revitalization of e-learning. Therefore, there are many studies for the contents searching method that make use of semantic web technology. Also, there are many studies to apply the ontology as the core technology of sematic web to e-learning. In this study, we bind the KEM(Korea Education Metadata) that enacted as the first technical standard of e-learning, KS X7001, in Dec. 2004 with OWL to use the metadata for a effective searching with ontology through the solving of the problem of metadata. And, we discuss some problems that occur in progress of binding and the solutions.

• Proceedings of the KIEE Conference
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• 2009.04a
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• pp.365-367
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• 2009

본 논문에서는 전기공사 단위시방서 작성 및 운영에 필요한 KEM-SPEC. 운영시스템을 개발하였다. KEM-SPEC. 운용시스템은 전기설비 시공에 있어, 필요한 공사시방서 작성 시, 누락과 오류, 중복적 피해를 최소화 하고, 전기공사 시공품질과 성능을 확보할 수 있다.