• Journal of Environmental Science International
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• v.19 no.9
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• pp.1143-1152
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• 2010

The purpose of this study is to degradation of Rhodamine B (RhB, dye) and N, N-Dimethyl-4-nitrosoaniline (RNO, indicator of the electro-generation of OH radical) in solution using boron doped diamond (BDD) electrode. The effects of applied current (0.2~1.0 A), electrolyte type (NaCl, KCl, and $Na_2SO_4$) and electrolyte concentration (0.5~3.0 g/L), solution pH (3~11) and air flow rate (0~4 L/min) were evaluated. Experimental results showed that RhB and RNO removal tendencies appeared with the almost similar thing, except of current. Optimum current for RhB degradation was 0.6 A, however, RNO degradations was increased with increase of applied current. The RhB and RNO degradation of Cl type electrolyte were higher than that of the sulfate type. The RhB and RNO degradation were increased with increase of NaCl concentration and optimum NaCl dosage was 2.5 g/L. The RhB and RNO concentrations were not influenced by pH under pH 7. Optimum air flow rate for the oxidants generation and RhB and RNO degradation were 2 L/min. Initial removal rate of electrolysis process was expressed Langmuir - Hinshelwood equation, which is used to express the initial removal rate of UV/$TiO_$2 process.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.2 no.2
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• pp.93-104
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• 1969

사후 저장기간 중 어육내에 진행되는 단백질 분해에 어육 자체효소와 새균활동 중 어느 쪽이 기여하는 바 큰가를 분별하기 위하여 금방 죽인 볼낙류(Sebastodes)의 어육을 무균방법으로 잘라내어 4 OZ 유리용기에 넣고 플라스틱으로 된 나사뚜껑으로 밀봉한 후 감마선 조사하지 않는 군(무균육과 무군육에 단백질 분해성 Pseudomonas 를 접종한 것으로 세분)과 0.5과 2.0 Mrad 선량 조사한 군(무균육, 조사 전에 접종시킨 어육, 그리고 조사후에 접종시킨 어육으로 세분)으로 나누며 $0^{\circ}C$에서의 저장 중의 어육의 총균수, pH, 관능 상해, 그리고 0.5M KCl 및 $70\%$ ethanol 가용성 총질소와 Amino-N을 검측함으로써 어육 단백질 분해 과정을 조사하였다. 0.5 Mrad 감마선량은 무균 어육에 접종된 $10^6$ per gm Muscle Pseudomonas sp.를 완전 사멸시키는데 충분하였으므로 완전한 무균상태와 처음부터 단백질 분해성 세균활동이 활발한 상태에 놓여 있는 어육 내에 진행되는 단백질 분해과정을 비교할 수 있었다. 기름지지 않는 어류에 속하는 볼박 어육의 저장 중에 일어나는 pH의 상승, 관능상태의 저락, 그리고 단백질 분해에서 오는 총질소와 Amino-N의 축적은 모두 세균의 번식활동과 직접 관련되어 있으며, 완전 무균 어육의 단백질 분해는 전혀 검측되지 안했으므로 통상 저장중에 일어나는 어육 담백질 분해는 전부가 세균활동에 기인됨이 확실해 졌다. 감마선 조사 직후 단백질 분해성 세균을 접종시킨 어육을 조사치 않고 접종시킨 어육에 비교할 때, 전자의 경우 세균번식이 현저히 장해 받았으며, 이에 따라 PH 상승과 총질소 및 Amino-N 축적도 상대적으로 부진하였다. 이 방사선을 조사받은 어육에 접종된 단백질 분해성 세균활동의 장해는 조사선량에 비례함이 밝혀졌다. 관능상태로 판단할 때 0.5 Mrad선을 조사받은 어육은 조사 받지 않은 것보다 선도유지기간이 2배 이상으로 연장되었다. 그러나 2.0 Mrad 선량 조사받은 어육의 선도유지는 오히려 조사 받지 않은 것보다 못하였다. 이 고선량 조사로 인한 식품의 물리적 및 화학적 변화의 해독성은 접종시킨 세균 번식 장해현상으로 나타났으며, 이 현상은 조사식품의 안전성 검측을 위한 하나의 도구로 사용될 가능성을 말해준다.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.3
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• pp.666-671
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• 2008

This study was undertaken to define the effect of ChungGongGo extract on norepinephrine-induced arterial contraction in rabbit. In order to investigate the effect of ChungGongGo extract on rabbit's contracted vascular ring detached from common carotid artery, vascular ring with intact or damaged endothelium was used for the experiment using organ bath. To analyze the mechanism of ChungGongGo extract-induced relaxation, ChungGongGo extract was infused into contracted vascular ring which had been pretreated by $N{\omega}$-nitro-L-arginine(L-NNA), Methylene blue(MB), and $Ca^{2+}$ was infused into contracted vascular ring induced by NE or KCl after treatment of ChungGongGo in $Ca^{2+}$-free solution. The results were as follows: ChungGongGo extract had an effective relaxation to the contracted vascular ring by NE in 1.0mg/ml and 0mg/ml level. ChungGongGo extract had an effective relaxation to the intact endothelium vascular ring, but when endothelium was removed, vascular ring did not relax. ChungGongGo extract-induced relaxation was inhibited by the pretreatment of L-NNA and MB. Pretreatment of ChungGongGo extract inhibit the contraction by influx of extra-$Ca^{2+}$ in contracted vascular ring induced by NE in $Ca^{2+}$-free solution. As mentioned above, we suggest that ChungGongGo relaxes vascular ring through suppress influx of extra-cellular $Ca^{2+}$ by the action of nitric oxide from endothelium.

• Journal of Plant Biology
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• v.39 no.1
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• pp.41-48
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• 1996

Two forms of homo serine dehydrogenase have been isolated from 8-day-old cotyledons of Canavalin lineata by a heat denaturation, ammonium sulfate fractionation, DEAE-8ephacel ion exchange and Sephacryl 8-300 gel filtration chromatographies, and Pro cion red dye, Cibacron blue dye and Resource Q column chromatographies. The molecular weights of T -form (threonine-sensitive) and K-form(threonine- insensitive) were estimated to 230 kD and 135 kD, respectively. In the presence of 10 mM threonine, the activity of T-form was inhibited with almost 70%, but that of K-form was not at all. The Km values tor homo serine of T- and Kform were 1.6 mM and 0.3 mM, respectively. The Km values for NAD of T- and K-form were 2.34 mM and 0.03 mM, respectively. And Km values for NADP of two isozymes were the same as 0.01 mM. The activities of T- and K-form were markedly stimulated up to 4.9and 2.8-fold, respectively, by 400 mM KCI. The partial purified(gel filtration) enzymes(Tform and K-form) can be reversibly converted.verted.

• BMB Reports
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• v.37 no.4
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• pp.466-473
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• 2004

A new acid deoxyribonuclease (DNase) was purified from the cultured mycelia of Cordyceps sinensis, and designated CSDNase. CSDNase was purified by $(NH_4)_2SO_4$ precipitation, Sephacryl S-100 HR gel filtration, weak anion-exchange HPLC, and gel filtration HPLC. The protein was single-chained, with an apparent molecular mass of ca. 34 kDa, as revealed by SDS-PAGE, and an isoelectric point of 7.05, as estimated by isoelectric focusing. CSDNase acted on both double-stranded (ds) and single- stranded (ss) DNA, but preferentially on dsDNA. The optimum pH of CSDNase was pH 5.5 and its optimum temperature 55. The activity of CSDNase was not dependent on divalent cations, but its enzymic activity was inhibited by high concentration of the cation: $MgCl_2$ above 150 mM, $MnCl_2$ above 200 mM, $ZnCl_2$ above 150 mM, $CaCl_2$ above 200 mM, NaCl above 300 mM, and KCl above 300 mM. CSDNase was found to hydrolyze DNA, and to generate 3-phosphate and 5-OH termini. These results indicate that the nucleolytic properties of CSDNase are essentially the same as those of other well-characterized acid DNases, and that CSDNase is a member of the acid DNase family. To our knowledge, this is the first report of an acid DNase in a fungus.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.9 no.6
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• pp.1760-1767
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• 2008

In this study, we analyzed the physical and chemical properties such as proximate analysis, ultimate analysis, heating values, and thermogravimetric analysis for the organic sludges discharged from an industrial complex in Ulsan. The average water, combustible, and ash content of organic sludges were 72.9, 18.5, and 8.6%, respectively. And according to the ultimate analysis of organic sludges, the C, O, H, N, and S compositions were 33.9, 26.4, 4.4, 4.4, and 0.6%, respectively. According to the results of investigating the lower heating values, 6 sludges were on the range of $1,500{\sim}2,000\;kcal/kg$ and 4 sludges were on the range of over 2,000 kcal/kg. Therefore, these 10 sludges could be directly applied to industries which try to use the energy by direct incineration.

• Restorative Dentistry and Endodontics
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• v.17 no.1
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• pp.1-9
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• 1992

The intradental nerve activity was recorded from single pulp nerve unit dissected from the inferior alveolar nerve in canine teeth of anesthetized cats. The effects of various test solutions on intradental nerve activity evoked by 4M NaCl applied to the deep dentinal cavities were investigated. 1. Total 15 single pulp nerve units were recorded. Of these 9 were Mi - fibers and 6 were C -fibers. The mean conduction velocity and electrical threshold of $A{\delta}$ - fiber were $6.3{\pm}3.7m/sec$, $1.2{\pm}0.7V$ and those of C - fiber were $1.0{\pm}0.2m/sec$, $2.3{\pm}1.3V$, respectively. The response to cold stimuli of $A{\delta}$ - fiber began immediately and that of C - fiber began after a latency. 2. When applied to deep dentinal cavity, 4M NaCl induced irregular bursts of action potential in 4 $A{\delta}$ - fibers and 3 C - fibers, which continued until the solution was washed away. 3. In the $A{\delta}$ - fiber, histamine failed to induce any nerve acitivity and did not produce an increase in intradental nerve activity evoked by 4M NaCl. However following the application of 1M KCl, the response to 4M NaCl was eliminated. 4. In the C - fiber, histamine generated some nerve activity and produced a significant increase in intradental nerve activity evoked by 4M NaCl, but 5M $CaCl_2$ did not abolish this enhandced response.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.20 no.2
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• pp.193-207
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• 2022

Thermodynamically, TRUO_x, REO_x, and SrO_x can be chlorinated using ammonium chloride (NH₄Cl) as a chlorinating agent, whereas uranium oxides (U₃O₈ and UO₂) remain in the oxide form. In the preliminary experiments of this study, U₃O₈ and CeO₂ are reacted separately with NH₄Cl at 623 K in a sealed reactor. CeO₂ is highly reactive with NH₄Cl and becomes chlorinated into CeCl₃. The chlorination yield ranges from 96% to 100%. By contrast, U₃O₈ remains as UO₂ even after chlorination. We produced U/REO_x- and U/SrO_x-simulated fuels to understand the chlorination characteristics of the oxide compounds. Each simulated fuel is chlorinated with NH₄Cl, and the products are dissolved in LiCl-KCl salt to separate the oxide compounds from the chloride salt. The oxide compounds precipitate at the bottom. The precipitate and salt phases are sampled and analyzed via X-ray diffraction, scanning electron microscope-energy dispersive spectroscopy, and inductively coupled plasma-optical emission spectroscopy. The analysis results indicate that REO_x and SrO_x can be easily chlorinated from the simulated fuels; however, only a few of U oxide phases is chlorinated, particularly from the U/SrO_x-simulated fuels.

• The Plant Pathology Journal
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• v.37 no.3
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• pp.215-231
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• 2021

Brown leaf spot disease caused by Nigrospora guilinensis on Phellodendron chinense occurs in a large area in Dayi County, Chengdu City, Sichuan Province, China each year. This outbreak has severely reduced the production of Chinese medicinal plants P. chinense and caused substantial economic losses. The bacterial isolate JKB05 was isolated from the healthy leaves of P. chinense, exhibited antagonistic effects against N. guilinensis and was identified as Bacillus megaterium. The following fermentation medium and conditions improved the inhibitory effect of B. megaterium JKB05 on N. guilinensis: 2% glucose, 0.1% soybean powder, 0.1% KCl, and 0.05% MgSO₄; initial concentration 6 × 10⁶ cfu/ml, and a 42-h optimal fermentation time. A composite of 0.1% nano-SiO₂ JKB05 improved the thermal stability, acid-base stability and ultraviolet resistance by 16%, 12%, and 38.9%, respectively, and nano-SiO₂ was added to the fermentation process. The best formula for the wettable powder was 35% kaolin, 4% polyethylene glycol, 8% Tween, and 2% humic acid. The following quality test results for the wettable powder were obtained: wetting time 87.0 s, suspension rate 80.33%, frequency of microbial contamination 0.08%, pH 7.2, fineness 95.8%, drying loss 1.47%, and storage stability ≥83.5%. A pot experiment revealed that the ability of JKB05 to prevent fungal infections on P. chinense increased considerably and achieved levels of control as high as 94%. The use of nanomaterials significantly improved the ability of biocontrol bacteria to control this disease.

• Korean Journal of Soil Science and Fertilizer
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• v.36 no.1
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• pp.1-7
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• 2003

Time domain reflectometry (TDR) is a newly developed method for measuring simultaneously solute concentrations and volumetric water content of soil. Bulk electrical conductivity ($EC_a$) of soil is obtained from TDR signal using several equations proposed, and electrical conductivity of soil solution ($EC_w$) can be calculated using the linear relationship $EC_a=EC_w\theta(a\theta+b)+EC_s$ between $EC_a$ and $EC_w$ at constant soil water content. The objectives of this study were to evaluate $EC_a$ proposed by several workers and to obtain the empirical constants (a, b, and $EC_s$) for $EC_w$ of the soils from A, Bl, and B2 horizon of an agricultural field (Coarse loamy, Fluvaquentic Eutrudepts). The $EC_a$ proposed by Yanuka et al. responded most sensitively to the KCl solute concentrations. The empirical constants of a, b, and $EC_s$ for $EC_w$ were -0.249, 1.358, and 0.054 for A horizon, -2.518, 2.708, and 0.097 for Bl horizon, and 2.490, -0.250, and 0.103 for B2 horizon, respectively. Therefore, the results of this study showed that Yanuka et al. equation was most useful one in determining $EC_a$, from TDR signal for agricultural soil with low salinity and that the empirical constants for the calculation of $EC_w$, from $EC_a$ can be obtained through a simple calibration experiment.