• KSBB Journal
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• v.23 no.5
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• pp.403-407
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• 2008

Recently, Candida antarctica lipase B (CalB) draws attention from industries for various applications for food, detergent, fine chemical, and biodiesel, because of its characteristics as an efficient biocatalyst. Since many industrial processes carry out in organic solvent and at high temperature, CalB, which is stable under harsh condition, is in demand from many industries. In order to reform CalB promptly, the expression system which has advantages of ease to use and low cost for gene libraries screening was developed using E. coli. The E. coli strains, Rosettagami with competence for enhanced disulfide bond formation, Novablue, and $DH5{\alpha}$, were exploited in this study. To obtain the soluble CalB, the pCold I vector expressing the cloned gene at $15^{\circ}C$ and the chaperone plasmids containing groES/groEL, groES/groEL/tig, tig, dnaK/dnaJ/grpE, and dnaK/dnaJ/grpE/groES/groEL were used for coexpression of CalB and chaperones. The colonies expressing functional lipase were selected by employing the halo plate containing 1% tributyrin, and the CalB expression was confirmed by SDS-PAGE. E. coli Rosettagami and $DH5{\alpha}$ harbouring groES/groEL chaperones were able to express soluble CalB effectively. From a facilitative point of view, E. coli $DH5{\alpha}$ is more suitable for further mutation study.

• Proceedings of the KIEE Conference
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• summer
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• pp.1474-1476
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• 2004

l5kW building integrated photovoltaic (BIPV) system had been installed and monitored at KIER in May 2003. Data acquisition system (DAS) is constructed for measuring and analyzing performance of PV system to observe the overall effect of environmental conditions on their operation characteristics. Performances of BIPV system have been evaluated and analyzed for component perspective (PV module and array, power conditioning system) and global perspective (system efficiency, capacity factor, electrical power energy) by long-term field test.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.24 no.5
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• pp.428-432
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• 2012

In the waste collection system, living waste is collected through the piping network. There are many confluent pipes in a piping network. These confluent pipes were often clogged up with waste. The optimal configuration of the confluent pipes has been defined by the design guide. However, nobody know how this design guide were determined. Therefore, in this paper, we found the design data for the confluent pipe configuration in order to avoid the waste clogging in a piping network. The distance between connected point on the confluent pipes should be longer than the previous design guide.

• Structural Engineering and Mechanics
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• v.11 no.5
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• pp.547-564
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• 2001

The numerical formulation of a two-phase interface element appropriate for porous lining system is presented. The formulation is isoparametric and can be applied both for 2-D and 3-D analysis. Biot's theory is utilized as the basis for the development of the element constitutive theory. In order to be capable of simulating the reinforcing characteristics of some geotextiles utilized as lining system, a reinforcement component has also been implemented into the formulation. By employing this specially developed interface finite element, the influence of soil consolidation on the stress distribution along the lining system of a reservoir and a landfill has been investigated.

• Journal of the Korea Institute of Military Science and Technology
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• v.21 no.5
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• pp.630-639
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• 2018

This paper proposes the design and implementation of adaptive naval gun fire simulator on a naval combat system. The proposed simulator can log data, analysis logged data, modify the BCU(Ballistic Computing Unit) S/W in real-time, and evaluate gun fire performance to check it satisfy requirement or not. When the simulation result satisfies the requirement, the BCU S/W is installed on onboard system. The simulation results show that similar result with actual naval gun fire reslult.

• Applied Chemistry for Engineering
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• v.5 no.5
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• pp.904-909
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• 1994

Cure kinetics of DGEBA(diglycidyl ether of bisphenol A)/MDA(4,4'-methylene dianiline)/GN(glutaronitrile) system with and without HQ(hydroquinone) as a catalyst was studied by Kissinger equation and Fractional life method. The activation energy of the system with HQ was somewhat lower and the pre-exponential factor of that was higher by about 30% than those of the system without HQ. As 1.25phr of HQ was added, reaction rates increased about 1.8 times.

• Journal of the Korea Institute of Military Science and Technology
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• v.16 no.5
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• pp.583-589
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• 2013

In this paper, we proposed a system that efficiently provides support maps which includes the grid based terrain analysis information. To do this, we use the FDB which is defined as a GIS database that contains features with attributes attached to the features. The FDB is composed of a number of features and feature classes. In order to create support maps, it is necessary to classify feature classes that are associated with each support map and to search them in a grid map. The proposed system use a ontology model to classify semantically feature classes and the quad-tree data structure to find them in a grid map quickly. Therefore, our system is expected to be utilized for global path planning of UGV. In this paper, we show the possibility through an experimental implementation.

• Journal of Electrical Engineering and Technology
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• v.8 no.5
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• pp.969-974
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• 2013

Protection issues in distribution systems which are to be operated in open and closed-loop modes in a smart grid are studied and a generalized protection setting method is proposed to meet the new requirements. The developed setting method assumes a conventional over-current protection scheme for the system with reclosers and over-current relays. In a closed-loop mode, it identifies protective devices that have to be sacrificed in order to maximize protection coordination for bidirectional fault current flow. The proposed setting method has been tested on many systems with different complexity and is proved effective.

• Journal of Biosystems Engineering
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• v.35 no.5
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• pp.287-293
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• 2010

In order to optimize an agricultural tractor clutch mechanism system, its structural static and kinematic mechanism were analyzed. The operating force of the mechanical tractor clutch system is currently not appropriate to drive comfortably. So it is needed to reduce the clutch operating force by applying advanced engineering design techniques. In the present study, an optimization technology is applied to the design of tractor clutch systems to reduce the operating force. As a result of the optimization using 2 link-angles and 1 link-length which are the main design variables of the clutch linkage system, the maximum pushing force of the maximum clutch pedal was found 182.8N, 14% decreased compared to the existing clutch system. The effectiveness of the optimum design is certified by menas of an experiment.

• Journal of Electrical Engineering and Technology
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• v.12 no.5
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• pp.2043-2050
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• 2017

This paper presents two MPC (Model Predictive Control) based charging and discharging algorithms of BESS (Battery Energy Storage System) for capacity firming of wind generation. To deal with the intermittency of the output of wind generation, a single BESS is employed. The proposed algorithms not only make the output of combined systems of wind generation and BESS track the predefined reference, but also keep the SoC (State of Charge) of BESS within its physical limitation. Since the proposed algorithms are both presented in simple if-then statements which are the optimal solutions of related optimization problems, they are both easy to implement in a real-time system. Finally, simulations of the two strategies are done using a realistic wind farm library and a BESS model. The results on both simulations show that the proposed algorithms effectively achieve capacity firming while fulfilling all physical constraints.