• Title/Summary/Keyword: K2PS

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Effect of Salicornia herbacea Polysaccharides on the Activation of Immune Cells in vitro and in vivo

  • Ryu, Deok-Seon;Kim, Seon-Hee;Lee, Dong-Seok
    • Food Science and Biotechnology
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    • v.18 no.6
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    • pp.1481-1486
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    • 2009
  • The immunomodulating effect of Salicornia herbacea polysaccharides on BALB/c mice splenocytes was investigated. Crude (CS) and fine polysaccharide (PS) extracts with potential biological activity were prepared from S. herbacea. For in vitro experiments, splenocytes and separated T cells were treated with CS and PS (0.5, 1, 2, and 4 mg/mL). For in vivo experiments, the CS and PS were orally administered to BALB/c mice every day for 2 weeks. For basic data analysis, physiological parameters were recorded. Cell proliferation of splenocytes and T cells was used as an index for immunomodulating activity. The proliferation of splenocytes and separated T cells was 3.2 and 3.5 times higher than the control, respectively. Moreover, when splenocytes were treated with mitogen, the highest proliferation rate was observed in splenocytes cultured with PS. Interestingly, the stimulative activity of PS was more strongly exerted through $CD4^+$ T cells than through $CD8^+$ T cells.

Optimization of Culture Conditions for Production of Pneumococcal Capsular Polysaccharide Type I

  • Kim, Su-Nam;Min, Kwan-Ki;Kim, Seung-Hwan;Choi, In-Hwa;Lee, Suhk-Hyung;Pyo, Suhk-Noung;Rhee, Dong-Kwon
    • Journal of Microbiology
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    • v.34 no.2
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    • pp.179-183
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    • 1996
  • Streptoccus Pneumoniae (pneumococcus), the most common cause of bacterial pneumonia, has an ample polysaccharide (PS) capsule that is highly antigenic and is the source of PS vaccine. This investigation was undertaken to optimize the culture conditions for the production of capsulard PS by type 1 pneumococcus. Among several culture media, brain heart infusion (BHI) and Casitone based media were found to support luxuriant growth of pneumococcus type 1 at the same level. Because BHI medium is rather expensive and more complex than the Casitone based media, the Casitone based media was uwed to study optimization of the culture condition. The phase of growth which accomodated maximum PS production was logarithmic phase. Concentrations of glucose greater than 0.2% did not ehnahce growth or PS production. Substitution of netrogen sources with other resources or supplementation of various concentrations of metal ion (with the exception of calcium ion) had adverse affects on growth and PS production. On the other hand, low level aeration was beneficial for increased PS production. Addition of 3 mg/1 concentration of methionine, phenylalanine, and threonine were found to enhance growth and PS production. The synerigistic effect of all the favorable conditions observed in pneumococcal growth assays provided a two-fold cummulative increase in capsular PS production.

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Preparation of Solid-Phase Extractant by Immobilizing Di-(2-ethylhexyl)phosphoric Acid (D2EHPA) and Tri-butyl-phosphate (TBP) in Polysulfone and Removal Characteristics of Cu(II) (Polysulfone에 추출제 Di-(2-ethylhexyl)phosphoric acid (D2EHPA)와 tri-butyl-phosphate(TBP)를 고정화한 고체상 추출제의 제조와 Cu(II)의 제거 특성)

  • Kam, Sang-Kyu;Jeon, Jin-Woo;Lee, Min-Gyu
    • Journal of Environmental Science International
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    • v.24 no.1
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    • pp.1-7
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    • 2015
  • The solid-phase extractant PS-D2EHPA/TBP was prepared by immobilizing two extractants D2EHPA and TBP in polysulfone (PS). The prepared PS-D2EHPA/TBP was characterized by using fourier transform infrared spectrometer (FTIR) and scanning electron microscopy (SEM). The removal of Cu(II) from aqueous solution was investigated in batch system. The experiment data were obeyed the pseudo-second-order kinetic model. Equilibrium data were well fitted by Langmuir model and the removal capacity of Cu(II) by solid extractant PS-D2EHPA/TBP obtained from Langmuir model was 3.11 mg/g at 288 K. The removal capacity of Cu(II) was increased according to increasing pH from 2 to 6, but the removal capacity was decreased below pH 3 remarkably.

Design of a Low Phase Noise Voltage Tuned Planar Composite Resonator Oscillator Using SIW Structure (SIW 구조를 이용한 저 위상잡음 전압 제어 평판형 복합공진기 발진기 설계)

  • Lee, Dong-Hyun;Son, Beom-Ik;Yeom, Kyung-Whan
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.25 no.5
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    • pp.515-525
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    • 2014
  • In this paper, we present a design and implementation of a Voltage-tuned Planar Composite Resonator Oscillator(Vt-PCRO) with a low phase noise. The designed Vt-PCRO is composed of a resonator, two phase shifters, and an amplifier. The resonator is designed using a dual mode SIW(Substrate Integrated Waveguide) resonator and has a group delay of about 40 nsec. Of the two phase shifters (PS1 and PS2), PS1 with a phase shift of $360^{\circ}$ is used for the open loop gain to satisfy oscillation condition without regard to the electrical lengths of the employed microstrip lines in the loop. PS2 with a phase shift of about $70^{\circ}$ is used to tune oscillation frequency. The amplifier is constructed using two stages to compensate for the loss of the open loop. Through the measurement of the open loop gain, the tune voltage of the PS1 can be set to satisfy the oscillation condition and the loop is then closed to form the oscillator. The oscillator with a oscillation frequency of 5.345 GHz shows a phase noise of -130.5 dBc/Hz at 100 kHz frequency offset. The oscillation power and the electrical frequency tuning range is about 3.5 dBm and about 4.2 MHz for a tuning voltage of 0~10 V, respectively.

Porous silicon-based chemical and biosensors (다공질 실리콘 구조를 이용한 화학 및 바이오 센서)

  • Kim, Yun-Ho;Park, Eun-Jin;Choi, Woo-Seok;Hong, Suk-In;Min, Nam-Ki
    • Proceedings of the KIEE Conference
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    • 2005.07c
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    • pp.2410-2412
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    • 2005
  • In this study, two types of PS substrate were fabricated for sensing of chemical and biological substances. For sensing of the humidity and chemical analyzes such as $CH_3OH$ or $C_2H_5OH$, PS layers are prepared by photoelectrochemical etching of silicon wafer in aqueous hydrofluoric acid solution. To evaluate their sensitivity, we measured the resistance variation of the PS diaphragm. As the amplitude of applied voltage increases from 2 to 6Vpp at constant frequency of 5kHz, the resistance variation for humidity sensor rises from 376.3 to $784.8{\Omega}$/%RH. And the sensitivities for $CH_3OH$ and $C_2H_5OH$ were 0.068 uA/% and 0.212 uA/%, respectively. For biological sensing application, amperometric urea sensors were fabricated based on porous silicon(PS), and planar silicon(PLS) electrode substrates by the electrochemical methods. Pt thin film was sputtered on these substrates which were previously formed by electrochemical anodization. Poly (3-methylthiophene) (P3MT) were used for electron transfer matrix between urease(Urs) and the electrode phase, and Urs also was by electrochemically immobilized. Effective working area of these electrodes was determined for the first time by using $Fe(CN)_6^{3-}/Fe(CN)_6^{4-}$ redox couple in which nearly reversible cyclic voltammograms were obtained. The $i_p$ vs $v^{1/2}$ plots show that effective working electrode area of the PS-based Pt thin film electrode was 1.6 times larger than the PLS-based one and we can readily expect the enlarged surface area of PS electrode would result in increased sensitivity by ca. 1.6 times. Actually, amperometric sensitivity of the Urs/P3MT/Pt/PS electrode was ca 0.91uA/$mM{\cdot}cm^2$, and that of the Urs/P3MT/Pt/PLS electrode was ca. 0.91uA/$mM{\cdot}cm^2$ in a linear range of 1mmol/L to 100mmol/L urea concentrations

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Evaluation of Yield and Quality from Red Pepper for Application Rates of Pig Slurry Composting Biofiltration (고추에서 SCB액비 시용량 설정을 위한 수량 및 품질 평가)

  • Lim, Tae-Jun;Hong, Soon-Dal;Kim, Seung-Heui;Park, Jin-Myeon
    • Korean Journal of Environmental Agriculture
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    • v.27 no.2
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    • pp.171-177
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    • 2008
  • The application in agricultural fields of pig slurry composting biofiltraton amending smell and nutrient unevenness, it is important for the appropriate nitrogen nutrient management to promote the availability of the crops and to minimize the risk of adversely environmental effects. The objective of this study was to determine the application rates of the preplant pig slurry composting biofiltration for red pepper(Capsicum annuum L.) by considering the yield response and the fruit quality such as sugar, capsaicinoid content. Red peppers were grown on plastic film ground under five different pig slurry(PS) application rates and mineral fertilizer(MF 100%) as a control. The effects of a single application of five different doses of PS: PS 0%(no kg N $ha^{-1}$), PS 50%(51.5 kg N $ha^{-1}$), PS 75%(77.3 kg N $ha^{-1}$), PS 100%(103 kg N $ha^{-1}$) and PS 125%(129 kg N $ha^{-1}$) were compared with the recommended mineral treatment(103 kg N $ha^{-1}$) in the pre-planting. The sidedressing N application(87 kg N $ha^{-1}$) was applied to the mineral fertilizer in all treatments. Red peppers were harvested at the mature red stage through five times from 72 days after transplanting(DAT) to 133 DAT. The results indicated that the yield of red pepper was increased with the increase of the N application rates from PS 0% to PS 100%. The highest yield was obtained in PS 100% by 20,843 kg $ha^{-1}$, although there were no significant differences in yield among PS 100, PS 125% and MF 100%. In addition, The contents of soluble sugar and capsaicinoids were not significantly different in all treatments. Accordingly, fertilization recommendations of red pepper to substitute PS for the mineral fertilizer were considered to PS 100%.

Effect of PEO of PS-P2VP photonic gel films

  • Shin, Sung-Eui;Kim, Su-Young;Shin, Dong-Myung
    • 한국정보디스플레이학회:학술대회논문집
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    • 2009.10a
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    • pp.1405-1407
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    • 2009
  • We prepared polystyrene-b-poly(2-vinyl pyridine) (PSb-P2VP) lamellar films which is hydrophobic block-hydrophilic polyelectrolyte block polymer have 57 kg/mol-b-57 kg/mol. The result of UV-visible absorption spectra supported that effect of poly(ethylene oxide) on the band gap tuning of PS-P2VP photonic gel like salt effect.

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Electronic Structures of ANb2PS10 (A=Ag, Na) and AuNb4P2S20

  • Jung, Dong-Woon;Kim, Sung-Jin
    • Bulletin of the Korean Chemical Society
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    • v.24 no.6
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    • pp.739-743
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    • 2003
  • New quaternary compounds $ANb_2PS_{10}$ (A = Na, Ag) and $AuNb_4P_2S_{20}$ were synthesized and characterized. The structures of three compounds consist of one-dimensional infinite chains built by [$Nb_2S_{12}$] and [$PS_4$] units. Cation atoms are occupied within the van der Waals gap of sulfur atoms between infinite chains to make -S…$M^+$…S- contacts. There is only one Au atom site and so crystallographically a unit cell contains four equivalent Au atoms in $AuNb_4P_2S_{20}$. This is only the half of the numbers of Na or Ag atoms in $NaNb_2PS_{10}$ or $AgNb_2PS_{10}$. The ratio between $Nb_2PS_{10}$ matrix vs the cation is, therefore, 1 : 1 for Ag and Na, but it is 2 : 1 for Au. Mixed valency in Au or Nb was expected to balance the charge in the latter compound. The electronic structures calculated based on the extended Huckel tight-binding method show that $ANb_2PS_{10}$ (A = Ag, Na) are semiconducting, while $AuNb_4P_2S_{20}$ is metallic, which is not consistent with the experimental results of these three compounds that all exhibit semiconducting property. The result of calculation suggests that $AuNb_4P_2S_{20}$ might be a magnetic insulator. Magnetic measurement experiment exactly proved that the compound is a Slater antiferromagnetic material with the Neels' temperature of 45 K. It is recognized, therefore, that electronic structure analysis is very useful to understand the properties of compounds.

OPTIMIZATION OF CULTURE CONDITIONS FOR PRODUCTION OF PNEUMOCOCCAL CAPSULAR POLYSACCHARIDE TYPE I

  • Kim, S.N.;K.K. Min;Kim, S.H.;Park, I.H.;Lee, S.H.;S.N. Pyo;D.K. Rhee
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1996.04a
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    • pp.186-186
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    • 1996
  • Streptococcus pneumoniae (pneumococcus), the most common cause of bacterial pneumonia, has an ample polysaccharide(PS) capsule that is highly antigenic and is the source of PS vaccine. This investigation was undertaken to optimize the culture conditions for the production of capsular PS by type 1 pneumococcus. Among several culture media, brain heart infusion (BHI) and Casitone based media were found to support luxuriant growth of pneumococcus type 1 at the same level. Because BHI medium is rather expensive and more complex than the Casitone based media, the Casitone based media was used to study optimization of the culture condition. The phase of growth which accomodated maximum PS production was logarithmic phase. Concentrations of glucose greater than 0.2% did not enhance growth or PS production. Substitution of nitrogen sources with other resources or supplemention of various concentrations of metal ion (with the exception of calcium ion) had adverse effects on growth and PS production. On the other hand, low level aeration was beneficial for increased PS production. Addition of 3 mg/I concentration of methionine, phenylalanine, and threonine were found to enhance growth and PS production. The synergistic effect of all the favorable conditions observed in pneumococcal growth assays provided a two-fold cumulative increase in capsular PS production.

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Universal Existence of One Chlorophyll a' Molecule in Photosystem I of Oxygenic Photosynthetic Organisms

  • Nakamura, Akimasa;Yoshida, Emi;Taki, Takashi;Watanabe, Tadashi
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.367-369
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    • 2002
  • Chlorophyll (ChI) a' is the Cl3$^2$-epimer of ChI a which is the constituent of P700, the primary electron donor of Photosystem (PS) I, of a thrmophilic cyanobacterium, Synechococcus elongatus, whose structure was recently determined by X-ray crystallography. To determine whether PS I of diverse oxygenic photosynthetic organisms universally contain one molecule of ChI a ’, pigment compositions of thylakoid membranes and PS I complexes isolated from cyanobacteria, green algae, red algae and higher plants were determined by reversed-phase HPLC. The results show that involvement of one ChI a'molecule in PS I is the universal feature for Chi a-based PS I of oxygenic photosynthetic organisms.

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