• Title/Summary/Keyword: K21

Search Result 50,099, Processing Time 0.067 seconds

Pcp-2 Interacts Directly with Kinesin Superfamily KIF21A Protein (Kinesin superfamily KIF21A와 직접 결합하는 Pcp-2의 규명)

  • Park, Hye-Young;Kim, Sang-Jin;Ye, Sung-Su;Jang, Won-Hee;Lee, Sang-Kyeong;Park, Yeong-Hong;Jung, Yong-Wook;Moon, Il-Soo;Kim, Moo-Seong;Seog, Dae-Hyun
    • Journal of Life Science
    • /
    • v.18 no.8
    • /
    • pp.1059-1065
    • /
    • 2008

  • KIF21A is a member of the Kinesin superfamily proteins (KIFs), which are microtubule-dependent molecular motors, anterograde axonal transporters of cargoes. Recently, congenital fibrosis of the extraocular muscles 1 (CFEOM1) has been shown to result from a small number of recurrent heterozygous missense mutations of KIF21A. CFEOM1 results from the inability of mutated KIF21A to successfully deliver cargoes to the development of the occulo-motor neuron or neuromuscular junction. Here, we used an yeast two-hybrid system to identify a protein that interacts with the WD-40 repeat domain of KIF21A and found a specific interaction with Purkinje cell protein-2 (Pcp-2), a small protein also known as L7. Pcp-2 protein bound to the WD-40 domain of KIF21A and KIF21B but not to other KIFs in yeast two-hybrid assays. In addition, this specific interaction was also observed in the glutathione S-transferase pull-down assay. An antibody to Pcp-2 specifically co-immunoprecipitated KIF21A associated with Pcp-2 from mouse brain extracts. These results suggest that Pcp-2 may be involved in the KIF21A-mediated transport as a KIF21A adaptor protein.

  • https://doi.org/10.5352/JLS.2008.18.8.1059 인용 PDF KSCI

Episcleritis in Eleven Dogs

  • Park, Shin-Ae;Jeong, Man-Bok;Kim, Won-Tae;Kim, Se-Eun;Chae, Je-Min;Park, Young-Woo;Son, Won-Gyun;Seo, Kang-Moon
    • Proceedings of the Korean Society of Veterinary Clinics Conference
    • /
    • 2007.05a
    • /
    • pp.91-91
    • /
    • 2007

  • PDF