• 제목/요약/키워드: K-Ras

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환경성 발암물질 및 유전자 다형성이 위암의 hMLH1 유전자 promoter의 과메틸화와 반복 서열 불안정성, 그리고 p53 및 Ki-ras 돌연변이에 미치는 영향에 대한 분자역학적 연구 (Effects of environmental carcinogens and genetic polymorphisms on the hypermethylation of hMLH1 gene promoter, microsatellite instability and mutations of the p53 and Ki-ras genes in gastric cancer)

  • 남홍매;박주승;윤효영;송영진;현태선;강종원;김헌
    • 대한예방의학회:학술대회논문집
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    • 대한예방의학회 2001년도 제53차 추계 학술대회 연제집
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    • pp.314-315
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    • 2001
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HEC-HMS와 HEC-RAS를 이용한 농촌 저지대 침수해석 (Flood Inundation Analysis in a Low-lying Rural Area using HEC-HMS and HEC-RAS)

  • 김학관;강문성;송인홍;황순호;박지훈;송정헌;김지혜
    • 한국농공학회논문집
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    • 제54권2호
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    • pp.1-6
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    • 2012
  • The objective of this study is to analyze the flood inundation in a low-lying rural area. The study watershed selected for this study includes the Il-Pae and Ahn-Gok watersheds. It is located in the Namyangju, Korea and encompasses $3.64km^2$. A major flood event that occurred in July 2011 was chosen as the case for the flood inundation analysis. The Hydrologic Engineering Center's Hydrologic Modeling System (HEC-HMS) and River Analysis System (HEC-RAS) were used to simulate flood runoff and water surface elevation at each cross-section, respectively. The watershed topographic, soil, and land use data were processed using the GIS (Geographic Information System) tool for the models. The contribution to the total flood volume was estimated based on the results simulated by HEC-HMS and HEC-RAS. The results showed that the overflow discharge from the Il-Pae stream constituted 80% of the total flood volume. The contributions of rainfall falling directly on the inundation area and overflow discharge from the Ahn-Gok stream were 15 % and 5 %, respectively. The simulation results in different levee scenarios for the Ahn-Gok stream were also compared. The results indicated that the levee could reduce the flood volume a little bit.

순환여과시스템에서 사육밀도와 용존산소 농도가 돌돔(Oplegnathus fasciatus)의 성장과 혈액성상에 미치는 영향 (Effects of Stocking Density and Dissolved Oxygen Concentration on the Growth and Hematology of the Parrotfish Oplegnathus fasciatus in a Recirculating Aquaculture System (RAS))

  • 김병기
    • 한국수산과학회지
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    • 제44권6호
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    • pp.747-752
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    • 2011
  • The parrotfish Oplegnathus fasciatus is a sub-tropical species that is difficult to culture during the winter in South Korea. As a result, a recirculating aquaculture system (RAS) was developed to rear parrotfish. This study investigated the effects of stocking density and dissolved oxygen (DO) concentration on the growth and hematology of the parrotfish in the RAS. The experimental stocking densities were 5 (SD05), 10 (SD10), 15 (SD15), and 20 kg/m3 (SD20) total body weight to tank water volume. As the stocking density increased, the mean weight gain, feed efficiency, and specific growth rate tended to decrease. However, SD10 and SD15 did not differ statistically from SD05 in feed efficiency (P>0.05). Although better growth was achieved in SD05, SD10 and SD15 appeared to be acceptable, practically and economically, in terms of feed efficiency. The experimental DO concentration ranges were 3-4 (DO3), 5-6 (DO5), and 7-8 mg/L (DO7). The mean weight gain and survival tended to increase with the DO concentration, but there were no differences among treatments (P>0.05). Although the feed efficiency did not differ among the treatments, the specific growth rates and daily feed intake increased with the DO concentration and were the highest in DO7 (P<0.05). The cortisol concentration was the highest in DO3 (P<0.05), while there was no difference between DO5 and DO7 (P>0.05).

한국소비자원 위해정보를 활용한 제품 리스크 평가시스템 개발 (Development of a Product Risk Assessment System using Injury Information in Korea Consumer Agency)

  • 서정대
    • 디지털융복합연구
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    • 제15권4호
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    • pp.181-190
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    • 2017
  • 최근 가습기살균제 사건을 비롯하여 휴대폰, 유아용 기저귀 등 생활용품의 안전사고가 빈번히 발생하고 있다. 이러한 사고로부터 소비자를 보호하기 위해서는 제품 안전관리가 필요하며, 제품의 안전성 정도를 평가할 수 있는 제품 리스크 평가 도구가 필요하다. 본 논문은 한국소비자원의 제품 사고관련 위해정보를 바탕으로 제품 리스크를 평가할 수 있는 시스템인 RAS(Risk Assessment System)를 구축하였다. RAS는 사고관련 정보를 분석하는 위해정보 분석시스템과 이 시스템으로부터 도출된 정보를 활용하여 리스크를 평가하는 리스크 평가시스템으로 구성되어 있다. 리스크 평가과정에서 제품 리스크에 영향을 미치는 인과관계를 반영한 베이지안 네트워크 기반의 확률적 기법을 적용하였다. RAS를 사용하여 어린이 제품 33품목에 대해 평가를 실시했으며 EU RAPEX의 RAG의 평가결과와 비교해 보았다. 그 결과 본 연구의 RAS의 결과는 전반적으로 EU RAPEX RAG의 평가 결과에 비해 낮은 수준을 보임을 알 수 있었다. 추후과제로는 사고영향척도 입력값의 주관성 저감, 위해정보 분석시스템과 리스크 평가시스템의 연동 등을 들 수 있다.

Effects of ${\alpha}-,\;{\beta}-Adrenergic$, and Calcium Channel Blockers on Renin- Angiotensin System in Perfused Rat Heart

  • Park, Chang-Gyo
    • The Korean Journal of Physiology and Pharmacology
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    • 제2권1호
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    • pp.55-62
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    • 1998
  • ${\alpha},\;{\beta}-Adrenergics$, and calcium channels were known to be related to inducing cardiac hypertrophy. Recently, it was reported that the cardiac renin-angiotensin system (RAS) was an important factor in ventricular hypertrophy. The present study was aimed to investigate the effects of ${\alpha},\;{\beta}-adrenergic$, and calcium channel blockers that might be involved in the regulation of cardiac RAS. The reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of renin gene in the perfused rat heart. Changes in angiotensin converting enzyme (ACE) activity and cyclic AMP (cAMP) content which were thought to play a role in inducing cardiac hypertrophy were measured in the perfused rat heart. The expression of renin gene was not only increased by isoproterenol with metoprolol-pretreatment but also increased by vasopressin treatment in the presence of calcium channel blocker, nifedipine or verapamil. Either prazosin alone or norepinephrine with prazosin-pretreatment significantly increased the ACE activity. However, isoproterenol with metoprolol-pretreatment significantly decreased the ACE activity. On the other hand, the ACE activity was not changed by vasopressin, nifedipine, or verapamil treatments. The content of cAMP was significantly increased by either isoproterenol or vasopressin treatment. According to these results, renin gene expression was associated with ${\beta}2$ - adrenoceptor and calcium channel. ACE activity was associated with ${\alpha}-\;and{\beta}2$ - adrenoceptor. In conclusion, ${\beta}2$ - adrenoceptor was important in cardiac renin gene expression and ACE activity and ${\alpha},\;{\beta}$ -adrenergic, and calcium channel blockers might be involved in the regulation of cardiac RAS in a complicated way.

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환소단이 영양혈청 결핍성 PC12 신경세포의 apoptosis에 미치는 영향 (Protective Effects of Hwansodan(Huanshao-dan) Water Extract in Serum Deprivation-induced Apoptosis of PC12 Cells)

  • 임준식;김명선;소홍섭;이지현;한상혁;허윤;박래길;문병순
    • 대한한의학회지
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    • 제21권4호
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    • pp.64-72
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    • 2000
  • Objectives : This study was designed to investigate the neuroprotective effect of Hwansodan(Huanshao-dan) on the apoptosis induced by withdrawal of neurotrophic support. Methods : PCl2 pheochromocytoma cells have been used extensively as a model for studying the cellular and molecular effects of neuronal cells. The viability of cells was measured by MTT assay. We used DNA fragmentation and caspase 3-like protease activation assay. Results : The water extract of Hwansodan(Huanshao-dan) significantly showed protective effects on serum and glucose deprivation-induced apoptotic death. Hwansodan(Huanshao-dan) also prevents DNA fragmentation and caspase 3-like protease activation, representing typical neuronal apoptotic phenomena in PCl2 pheochromocytoma cells and induces tyrosine phosphorylation of proteins around 44 kDa, which was identified as ERK1 with electrophoretic gel mobility shift by Western blot. In addition, MAPK kinase(MEK) inhibitor PD98059 and Ras inactivator, ${\alpha}-hydroxyfarnesylphosphonic$ acid attenuated the neuroprotective effects of Hwansodan(Huanshao-dan) in serum-deprived PCl2 cells. Conclusions : These results indicate that Ras/MEK/ERK signaling pathway plays a key role in neuroprotective effects of Hwansodan(Huanshao-dan) in serum-deprived PCl2 cells. Taken together, we suggest the possibility that Hwansodan(Huanshao-dan) might provide a neurotrophic-like activity in PCl2 cells.

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