• Asian Pacific Journal of Cancer Prevention
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• 제15권24호
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• pp.10825-10830
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• 2015

The present study was conducted to investigate effects and mechanisms of action of phenolic alkaloids of Menispermum dauricum (PAMD) on gastric cancer in vivo. In vitro, cell apoptosis of human gastric cancer cell line SGC-7901 was observed using fluorescence staining. In vivo, a mice model was constructed to observe tumor growth with different doses. Cell apoptosis was examined using flow cytometry and K-RAS protein expression using Western blotting. The mRNA expression of P53, BCL-2, BAX, CASPASE-3, K-RAS was examined by real-time PCR. PAMD significantly suppressed tumor growth in the xenograft model of gastric cancer in a dose-dependent manner (p<0.01). Functionally, PAMD promoted cell apoptosis of the SGC-7901 cells and significantly increased the rate of cell apoptosis of gastric tumor cells (p<0.05). Mechanically, PAMD inhibited the expression of oncogenic K-RAS both at the mRNA and protein levels. In addition, PAMD affected the mRNA expression of the cell apoptosis-related genes (P53, BCL-2, BAX, CASPASE-3). PAMD could suppress gastric tumor growth in vivo, possibly through inhibiting oncogenic K-RAS, and induce cell apoptosis possibly by targeting the cell apoptosis-related genes of P53, BCL-2, BAX, CASPASE-3.

• 대한한의학회지
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• 제33권2호
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• pp.73-77
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• 2012

Objectives: This report reports a clinical case of a patient with recurrent aphthous stomatitis (RAS) for 10 years who was completely cured using Korean medicine. Methods: A male patient with terribly severe RAS was hospitalized, and then treated with Jibaekpalmi-hwon, indirect moxibustion (KI 1 and CV 4) and acupuncture. The clinical outcome was observed by self-reported VAS measurement and macroscopy. Results: The severity of stomatitis became improved gradually and completely recovered during 17 hospital days, and RAS has not recurred for three months as an outpatient. The blood test including complement activity was in the normal range. Conclusions: This case report provides information about a therapeutic effect of Jibaekpalmi-hwon and Korean medicine on RAS.

• 한국분말재료학회지
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• 제27권6호
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• pp.464-467
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• 2020

Nickel nanopowders are obtained by the spark discharge method, which is based on the evaporation of the electrode surface under the action of the discharge current, followed by vapor condensation and the formation of nanoparticles. Nickel electrodes with a purity of 99.99% are used to synthesize the nickel nanoparticles in the setup. Nitrogen is used as the carrier gas with a purity of 99.998%. XRD, TEM, and EDX analyses of the nanopowders are performed. Moreover, HRTEM images with measured interplanar spacings are obtained. In the nickel nanopowder samples, a phase of approximately 90 wt% with an expanded crystal lattice of 6.5% on average is found. The results indicate an unusual process of nickel nanoparticle formation when the spark discharge method is employed.

• 대한예방의학회:학술대회논문집
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• 대한예방의학회 1999년도 제51차 추계 학술대회 연제집
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• pp.138-139
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• 1999

• 한국환경성돌연변이발암원학회:학술대회논문집
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• 한국환경성돌연변이발암원학회 2003년도 춘계학술대회
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• pp.100-101
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• 2003

• Bulletin of the Korean Chemical Society
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• 제26권2호
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• pp.327-330
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• 2005

• The Korean Journal of Physiology and Pharmacology
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• 제19권4호
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• pp.299-307
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• 2015

Severe acute pancreatitis (SAP) is normally related to multiorgan dysfunction and local complications. Studies have found that local pancreatic renin-angiotensin system (RAS) was significantly upregulated in drug-induced SAP. The present study aimed to investigate the effects of angiotensin II receptors inhibitor valsartan on dual role of RAS in SAP in a rat model and to elucidate the underlying mechanisms. 3.8% sodium taurocholate (1 ml/kg) was injected to the pancreatic capsule in order for pancreatitis induction. Rats in the sham group were injected with normal saline in identical locations. We also investigated the regulation of experimentally induced SAP on local RAS expression in the pancreas through determination of the activities of serum amylase, lipase and myeloperoxidase, histological and biochemical analysis, radioimmunoassay, fluorescence quantitative PCR and Western blot analysis. The results indicated that valsartan could effectively suppress the local RAS to protect against experimental acute pancreatitis through inhibition of microcirculation disturbances and inflammation. The results suggest that pancreatic RAS plays a critical role in the regulation of pancreatic functions and demonstrates application potential as AT1 receptor antagonists. Moreover, other RAS inhibitors could be a new therapeutic target in acute pancreatitis.

• 한국수자원학회논문집
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• 제45권9호
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• pp.959-968
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• 2012

주운수로 홍수기 수위 산정을 위하여 미 공병단에서 개발한 HEC-RAS 모형을 적용하였다. 아라뱃길은 다양한 수리구조물의 조작을 통하여 선박운항 및 홍수처리가 이루어지며 유입홍수, 한강수위, 그리고 서해조위 변동과 연계하여 귤현보, 서해배수문, 체절수문, 그리고 배수펌프장등의 대표적인 구조물 운영을 지원할 수 있는 홍수위 계산모형이 필요하다. 특히 서해배수문의 개도를 결정하기 위하여 외조위와 내수위 변동을 동시에 고려한 수문운영이 필요하여 HEC-RAS의 Rule-script 기능을 적용하여 이러한 특성을 반영하였다. 모형의 검보정을 위하여 2010년 9월 홍수사상을 적용하였으며 모형으로부터 계산된 수위값과 관측된 수위값이 서로 잘 일치함을 확인하였다. 본 연구에서 구축된 HEC-RAS모형은 홍수기 주운수로 운영에 기여할 것으로 판단된다.

• 천문학회지
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• 제35권4호
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• pp.209-220
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• 2002

In order to increase the completeness of the investigations of stellar abundances, we can use spectrum synthesis method, new atomic data and observation of stellar spectra with resolution comparable to solar spectral atlases. We made a brief review of main problems of these three ways. We present new results of abundance determinations in the atmospheres of four stars. The first is the implementation of new atomic data to well known Przybylski's star. We show that the number of spectral lines, which can be identificated in the spectrum of this star, can be significantly higher. The second example is the investigation of $\zeta$ Cyg. We found the abundances of 51 elements in the atmosphere of this mild barium star. The third example is halo star HD221170. Our preliminary abundance pattern consists of 42 elements. The heaviest elements in this pattern are U and Th. The last star is the spectroscopic binary HD153720. The number of elements investigated in the spectra of components of this star is not large, but the results show that the components are Am-stars.

• 생명과학회지
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• 제22권4호
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• pp.538-544
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• 2012

에탄올은 사람에 대한 발암물질로 잘 알려져 있다. 또한 여러 조직이나 세포에서의 에탄올에 의한 세포증식억제효과도 잘 알려져 있다. 본 연구에서는 여러 암세포에서 에탄올에 의한 세포증식억제 효과를 조사하였는데 특히 발암원성 $ras$로 형질전환되거나 미세주입된 세포에서의 영향을 조사하였다. 에탄올은 여러 정상세포들의 증식을 억제하였다. 반면에 여러 암세포나 발암원성 Ras에 의한 세포증식은 억제하지 못 하였다. 또한 발암원성 단백질의 세포내 미세주사에 의한 DNA합성 유도도 에탄올에 의해 억제 되지 않았다. 이러한 에탄올의 세포증식억제 효과는 $N$-acetylcysteine이나 4-methylpyrazole과 같은 항산화제에 의해 제거되었다. 이러한 실험 결과는 에탄올에 의한 세포증식억제 효과는 Ras단백질의 upstream에 있거나 또는 Ras와 독립적으로 작용하며, 활성산소 형성과 밀접한 관계가 있다는 것을 알려준다.