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http://dx.doi.org/10.5352/JLS.2012.22.4.538

Anti-Proliferative Effect of Ethanol on Normal and Cancer Cells  

Oh, Myung-Ju (Division of Clinical Trials Management, Bureau of Risk Prevention Policy, Korea Food and Drug Administration)
Kim, Ji-Hyun (Department of Nanomedical Engineering, Pusan National University)
Park, Su-Hyun (Department of Nanomedical Engineering, Pusan National University)
Jeong, Young-Hwa (Department of Nanomedical Engineering, Pusan National University)
Wang, Kun (Department of Animal Sciences, Pusan National University)
Cho, Byung-Wook (Department of Animal Sciences, Pusan National University)
Jhun, Byung-H. (Department of Nanomedical Engineering, Pusan National University)
Publication Information
Journal of Life Science / v.22, no.4, 2012 , pp. 538-544 More about this Journal
Abstract
Ethanol is known as being carcinogenic to humans. In addition, the anti-proliferative effects of ethanol have been described for a variety of tissues and cells. In this study, we investigated the anti-proliferative effects of ethanol on various cancer cells, particularly on oncogenic $ras$-transformed or-injected cells. Ethanol treatment inhibited the cell proliferation of normal control cells, but did not suppress the proliferation of various cancer cells and oncogenic $ras$-transformed cells. Furthermore, ethanol treatment did not interfere with DNA synthesis, which was induced by microinjecting the oncogenic $H-Ras^{V12}$ protein. The anti-proliferative effect of ethanol was rescued by antioxidants, such as $N$-acetylcysteine and 4-methlpyrazole. These results suggest that ethanol cytotoxicity is exerted through free radical formation, and that the anti-proliferative action site of ethanol cytotoxicity either lies upstream, or is independent of Ras.
Keywords
Ethanol; cell proliferation; Ras; microinjection; antioxidant;
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