• Title/Summary/Keyword: K-Ras

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In vitro Interaction of Recombinantly Expressed Kringle 5 (rK5) with Ras Guanine Nucleotide Dissociation Stimulator-like Factor (Rgl2)

  • Lee, Jung-Whoi;Kim, Sun-Hee;Park, Yong-Sung;Woo, Je-Wan;Lim, Dong-Yeol;Lee, Kyung-Hee
    • Bulletin of the Korean Chemical Society
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    • v.25 no.12
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    • pp.1863-1868
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    • 2004
  • Kringle 5 (K5), located outside of angiostain (K1-4) in human plasminogen, displays more potent antiangiogenic activity on endothelial cell proliferation than angiostatin itself. Using a yeast two-hybrid system in vivo, we have recently identified Rgl2 (guanine nucleotide dissociation stimulator (RalGDS)-like factor 2) as a binding protein of human K5. In order to confirm in vitro protein interaction between K5 and Rgl2, we developed bacterial recombinant expression systems for them. K5 and Rgl2 proteins were expressed in high yields and purified into pure forms with His tags and GST fusion, respectively. GST-pull down experiments clearly demonstrated that K5 interacts specifically with Rgl2 in vitro. These results indicate that Rgl2 functions as a receptor protein for K5 in vitro as well as in vivo, leading to anti-angiogenesis through regulating Ras signaling pathways.

Capsaicin-induced apoptosis in H-ras MCF10A cells involves activation of MAPkinases

  • Kang, Hye-Jung;Yunjo Soh;Lee, Eun-Jung;Kim, Mi-Sung;Surh, Young-Joon;Aree Moon
    • Proceedings of the Korean Society of Toxicology Conference
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    • 2001.05a
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    • pp.157-157
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    • 2001
  • Capsaicin (8-methyl-N-vanillyl-6-nonenamide), the principal pugent ingredient found in hot red pepper, has been recently known to have anticarcinogenic or chemopreventive properties. In the previous study, we showed that capsaicin selectively induces apoptosis in H-ras MCF10A cells.(omitted)

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The Propagation Effects on the National Economy induced by u-City Construction: I-O Analysis (u-City 구축에 따른 생산 파급효과 추정)

  • Kim, Pang-Ryong
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2007.06a
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    • pp.139-142
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    • 2007
  • 본 연구는 RAS 계수법과 동태적 산업연관 모형을 동시에 적용하여 u-City 구축의 경제적 파급효과를 분석하였다는 점에서 통상적인 산업연관분석과 차별성을 지닌다. 한국은행이 가장 최근 발표한 산업연관표는 2000년도 자료이다. 본 연구는 RAS 계수법으로 알려져 있는 예측기법을 이용하여 2000년 산업연관표를 2010년 표로 연장시켜 u-City 산업의 경제적 파급효과를 분석한다. 한편 산업연관분석에 통상적으로 사용되고 있는 모형은 정태적 분석모형인 기본 산업연관 모형이지만, 본 연구에서는 동태적 산업연관 모형을 적용하여 u-City 산업의 경제적 파급효과를 동태적으로 분석하였다. u-City 구축에 따른 총 생산파급효과는 약 3.3배로 추정되었으며, u-City 산업은 전방연쇄효과는 매우 높지만 후방연쇄효과는 그다지 높지 않은 것으로 나타났다.

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The System of Plasma Ignition for Coal-Dust and Water-Coal Fuels Ignition

  • Park, Hyun-Seo;I. M. Zasypkin;A. N. Timoshevskii
    • Resources Recycling
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    • v.12 no.2
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    • pp.54-61
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    • 2003
  • In this paper a system of plasma ignition(SPI) which is applied for the ignition and stabilization of coal-dust fuel burning for decreasing fuel black oil consumption is described. The advantages of SPI are demonstrated, and the positive results of SPI which is operated at the thermal-clamping boilers installed in production and heating plants are described. The similar system was tested in demonstration and industrial installations to confirm the results. The improvement of economical, operating and ecological performances of the boiler are shown.

Hydraulic Analysis Using a Two-Dimensional Model(I) : Flow Analysis around Bridge Piers with Pier Shapes (2차원 모형을 이용한 수리해석(I) : 교각형상별 주변부 흐름해석)

  • Kim, Eung-seok;Lee, Seung-hyun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.7
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    • pp.4936-4941
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    • 2015
  • This study(I) has analyzed hydraulic characteristics with pier shapes by the bridge construction. The pier shapes are classified into total six types such as square, rhombus, octagon, oval, round, and no-piers. One-dimensional model(HEC-RAS) and two-dimensional model (RMA-2) were employed to analyze hydraulic characteristics around bridge piers. Square and rhombus shapes of piers showed velocity vectors in the upstream direction, which has a significant impact on the river bed changes by erosion and sediment transport around the piers. The flow characteristics of the oval type pier was most similar to that of no-pier situation almost without disrupting the river flow. This analysis can help to select pier types in the new bridge construction for the future.