• Title/Summary/Keyword: K-Ras

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Effects of environmental carcinogens and genetic polymorphisms on the hypermethylation of hMLH1 gene promoter, microsatellite instability and mutations of the p53 and Ki-ras genes in gastric cancer (환경성 발암물질 및 유전자 다형성이 위암의 hMLH1 유전자 promoter의 과메틸화와 반복 서열 불안정성, 그리고 p53 및 Ki-ras 돌연변이에 미치는 영향에 대한 분자역학적 연구)

  • Nan Hong-Mei;Park Joo-Seung;Yun Hyo-Yung;Song Young-Jin;Hyun Tai-Sun;Kang Jong-Won;Kim Heon
    • 대한예방의학회:학술대회논문집
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    • 2001.10a
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    • pp.314-315
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    • 2001
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Flood Inundation Analysis in a Low-lying Rural Area using HEC-HMS and HEC-RAS (HEC-HMS와 HEC-RAS를 이용한 농촌 저지대 침수해석)

  • Kim, Hak-Kwan;Kang, Moon-Seong;Song, In-Hong;Hwang, Soon-Ho;Park, Ji-Hoon;Song, Jung-Hun;Kim, Ji-Hye
    • Journal of The Korean Society of Agricultural Engineers
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    • v.54 no.2
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    • pp.1-6
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    • 2012
  • The objective of this study is to analyze the flood inundation in a low-lying rural area. The study watershed selected for this study includes the Il-Pae and Ahn-Gok watersheds. It is located in the Namyangju, Korea and encompasses $3.64km^2$. A major flood event that occurred in July 2011 was chosen as the case for the flood inundation analysis. The Hydrologic Engineering Center's Hydrologic Modeling System (HEC-HMS) and River Analysis System (HEC-RAS) were used to simulate flood runoff and water surface elevation at each cross-section, respectively. The watershed topographic, soil, and land use data were processed using the GIS (Geographic Information System) tool for the models. The contribution to the total flood volume was estimated based on the results simulated by HEC-HMS and HEC-RAS. The results showed that the overflow discharge from the Il-Pae stream constituted 80% of the total flood volume. The contributions of rainfall falling directly on the inundation area and overflow discharge from the Ahn-Gok stream were 15 % and 5 %, respectively. The simulation results in different levee scenarios for the Ahn-Gok stream were also compared. The results indicated that the levee could reduce the flood volume a little bit.

Effects of Stocking Density and Dissolved Oxygen Concentration on the Growth and Hematology of the Parrotfish Oplegnathus fasciatus in a Recirculating Aquaculture System (RAS) (순환여과시스템에서 사육밀도와 용존산소 농도가 돌돔(Oplegnathus fasciatus)의 성장과 혈액성상에 미치는 영향)

  • Kim, Pyong-Kih
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.44 no.6
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    • pp.747-752
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    • 2011
  • The parrotfish Oplegnathus fasciatus is a sub-tropical species that is difficult to culture during the winter in South Korea. As a result, a recirculating aquaculture system (RAS) was developed to rear parrotfish. This study investigated the effects of stocking density and dissolved oxygen (DO) concentration on the growth and hematology of the parrotfish in the RAS. The experimental stocking densities were 5 (SD05), 10 (SD10), 15 (SD15), and 20 kg/m3 (SD20) total body weight to tank water volume. As the stocking density increased, the mean weight gain, feed efficiency, and specific growth rate tended to decrease. However, SD10 and SD15 did not differ statistically from SD05 in feed efficiency (P>0.05). Although better growth was achieved in SD05, SD10 and SD15 appeared to be acceptable, practically and economically, in terms of feed efficiency. The experimental DO concentration ranges were 3-4 (DO3), 5-6 (DO5), and 7-8 mg/L (DO7). The mean weight gain and survival tended to increase with the DO concentration, but there were no differences among treatments (P>0.05). Although the feed efficiency did not differ among the treatments, the specific growth rates and daily feed intake increased with the DO concentration and were the highest in DO7 (P<0.05). The cortisol concentration was the highest in DO3 (P<0.05), while there was no difference between DO5 and DO7 (P>0.05).

Development of a Product Risk Assessment System using Injury Information in Korea Consumer Agency (한국소비자원 위해정보를 활용한 제품 리스크 평가시스템 개발)

  • Suh, Jungdae
    • Journal of Digital Convergence
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    • v.15 no.4
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    • pp.181-190
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    • 2017
  • Recently, safety accidents of daily necessities such as humidifier disinfectant, mobile phones, and infant diapers, have occurred frequently. To protect consumers from these accidents, product safety management is required, and a product risk assessment tool is needed to evaluate the degree of safety of the product. In this paper, we have constructed RAS, which is a system that can evaluate product risk based on injury information of product accident in Korea Consumer Agency. RAS consists of an injury information analysis system for analyzing accident-related information and a risk assessment system for assessing risk using information derived from the system. The Bayesian network - based probabilistic method is applied to reflect the causal relationships that affect product risk in the risk assessment process. We used RAS to evaluate 33 children's products and compared them with the results of EU RAPEX RAG. Subsequent tasks include reducing the subjectivity of the input of the accident impact scale, and linking the above two systems.

Effects of ${\alpha}-,\;{\beta}-Adrenergic$, and Calcium Channel Blockers on Renin- Angiotensin System in Perfused Rat Heart

  • Park, Chang-Gyo
    • The Korean Journal of Physiology and Pharmacology
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    • v.2 no.1
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    • pp.55-62
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    • 1998
  • ${\alpha},\;{\beta}-Adrenergics$, and calcium channels were known to be related to inducing cardiac hypertrophy. Recently, it was reported that the cardiac renin-angiotensin system (RAS) was an important factor in ventricular hypertrophy. The present study was aimed to investigate the effects of ${\alpha},\;{\beta}-adrenergic$, and calcium channel blockers that might be involved in the regulation of cardiac RAS. The reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of renin gene in the perfused rat heart. Changes in angiotensin converting enzyme (ACE) activity and cyclic AMP (cAMP) content which were thought to play a role in inducing cardiac hypertrophy were measured in the perfused rat heart. The expression of renin gene was not only increased by isoproterenol with metoprolol-pretreatment but also increased by vasopressin treatment in the presence of calcium channel blocker, nifedipine or verapamil. Either prazosin alone or norepinephrine with prazosin-pretreatment significantly increased the ACE activity. However, isoproterenol with metoprolol-pretreatment significantly decreased the ACE activity. On the other hand, the ACE activity was not changed by vasopressin, nifedipine, or verapamil treatments. The content of cAMP was significantly increased by either isoproterenol or vasopressin treatment. According to these results, renin gene expression was associated with ${\beta}2$ - adrenoceptor and calcium channel. ACE activity was associated with ${\alpha}-\;and{\beta}2$ - adrenoceptor. In conclusion, ${\beta}2$ - adrenoceptor was important in cardiac renin gene expression and ACE activity and ${\alpha},\;{\beta}$ -adrenergic, and calcium channel blockers might be involved in the regulation of cardiac RAS in a complicated way.

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Protective Effects of Hwansodan(Huanshao-dan) Water Extract in Serum Deprivation-induced Apoptosis of PC12 Cells (환소단이 영양혈청 결핍성 PC12 신경세포의 apoptosis에 미치는 영향)

  • 임준식;김명선;소홍섭;이지현;한상혁;허윤;박래길;문병순
    • The Journal of Korean Medicine
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    • v.21 no.4
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    • pp.64-72
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    • 2000
  • Objectives : This study was designed to investigate the neuroprotective effect of Hwansodan(Huanshao-dan) on the apoptosis induced by withdrawal of neurotrophic support. Methods : PCl2 pheochromocytoma cells have been used extensively as a model for studying the cellular and molecular effects of neuronal cells. The viability of cells was measured by MTT assay. We used DNA fragmentation and caspase 3-like protease activation assay. Results : The water extract of Hwansodan(Huanshao-dan) significantly showed protective effects on serum and glucose deprivation-induced apoptotic death. Hwansodan(Huanshao-dan) also prevents DNA fragmentation and caspase 3-like protease activation, representing typical neuronal apoptotic phenomena in PCl2 pheochromocytoma cells and induces tyrosine phosphorylation of proteins around 44 kDa, which was identified as ERK1 with electrophoretic gel mobility shift by Western blot. In addition, MAPK kinase(MEK) inhibitor PD98059 and Ras inactivator, ${\alpha}-hydroxyfarnesylphosphonic$ acid attenuated the neuroprotective effects of Hwansodan(Huanshao-dan) in serum-deprived PCl2 cells. Conclusions : These results indicate that Ras/MEK/ERK signaling pathway plays a key role in neuroprotective effects of Hwansodan(Huanshao-dan) in serum-deprived PCl2 cells. Taken together, we suggest the possibility that Hwansodan(Huanshao-dan) might provide a neurotrophic-like activity in PCl2 cells.

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