• Journal of Plant Biotechnology
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• v.34 no.1
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• pp.7-10
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• 2007

Plant regeneration system from floral stem of Mymphoides indica via somatic embryogenesis was established. After four weeks of culture onto 1/2MS medium containing 2,4-D, pale-yellow globular structures and calluses were formed on the cut surface of floral stem explants. Upon transfer to 1/2MS basal medium, pale-yellow globular structures were developed into somatic embryos and normal plantlets. These results indicated that pale-yellow globular structures and calluses from floral stem were globular embryos and embryogenic calluses, respectively. The frequency of embryogenic callus formation from floral stem was reached to nearly 100% when floral stem was cultured onto 1/2Ms medium supplemented with low concentration of 2,4-D (0.1 to 0.3 mg/L). However, the higher concentration of 2,4-D resulted in decrease of the frequency of embryogenic callus formation. In this study, low concentration of 2,4-D had a stimulative role in embryogenic callus formation, whereas BA showed inhibitory role in callus formation. In comparison to floral stem, leaf explants showed low frequency of embryogenic callus formation. The highest frequency of embryogenic callus formation from leaf explants was 9.5% when leaf explants were cultured onto 1/2MS medium supplemented with 0.3 mg/L of 2,4-D. The plant regeneration system of Nymphoides indica established in this study, might be applied to mass proliferation, conservation of genetic resources and genetic transformation for molecular breeding.

• Journal of Plant Biotechnology
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• v.34 no.2
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• pp.145-152
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• 2007

Lactoferrin is an 80-kDa iron-binding glycoprotein known to exert many biological activities, such as facilitating iron absorption and having antimicrobial and anti-inflammatory effects. Rice can be a useful target for edible food plants to introduce human lactoferrin, because it has lower allergenicity and is likely to be safer than microorganisms or transgenic animals. A cDNA fragment encoding human lactoferrin (HLF) driven by the maize polyubiquitin promoter, along with herbicide resistance gene (bar) driven by CaMV 35S promoter, was introduced into rice (Oryza sativa L. cv. Dong Jin) using the Agrobacterium -mediated transformation system. Putative transformants were initially selected on the medium containing bialaphos. The stable integration of the bar and HLF genes into transgenic rice plants was further confirmed through polymerase chain reaction (PCR) and Southern blot analyses. The expression of the full length HLF protein from various tissues such as grains and young leaves of transgenic rice was verified by Western blot analysis. Analysis of progeny also demonstrated that introduced genes were stably inherited to the next generation at the Mendelian fashion.

• Journal of the Korean Mathematical Society
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• v.44 no.4
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• pp.1025-1050
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• 2007

Let $X_k(x)=({\int}^T_o{\alpha}_1(s)dx(s),...,{\int}^T_o{\alpha}_k(s)dx(s))\;and\;X_{\tau}(x)=(x(t_1),...,x(t_k))$ on the classical Wiener space, where ${{\alpha}_1,...,{\alpha}_k}$ is an orthonormal subset of $L_2$ [0, T] and ${\tau}:0 is a partition of [0, T]. In this paper, we establish a change of scale formula for conditional Wiener integrals $E[G_{\gamma}|X_k]$ of functions on classical Wiener space having the form $$G_{\gamma}(x)=F(x){\Psi}({\int}^T_ov_1(s)dx(s),...,{\int}^T_o\;v_{\gamma}(s)dx(s))$$, for $F{\in}S\;and\;{\Psi}={\psi}+{\phi}({\psi}{\in}L_p(\mathbb{R}^{\gamma}),\;{\phi}{\in}\hat{M}(\mathbb{R}^{\gamma}))$, which need not be bounded or continuous. Here S is a Banach algebra on classical Wiener space and $\hat{M}(\mathbb{R}^{\gamma})$ is the space of Fourier transforms of measures of bounded variation over $\mathbb{R}^{\gamma}$. As results of the formula, we derive a change of scale formula for the conditional Wiener integrals $E[G_{\gamma}|X_{\tau}]\;and\;E[F|X_{\tau}]$. Finally, we show that the analytic Feynman integral of F can be expressed as a limit of a change of scale transformation of the conditional Wiener integral of F using an inversion formula which changes the conditional Wiener integral of F to an ordinary Wiener integral of F, and then we obtain another type of change of scale formula for Wiener integrals of F.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.19 no.6
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• pp.557-564
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• 2007

The effect of wave and current interactions on regular wave transformation over a submerged elliptic shoal is investigated based on numerical simulations of the Vincent and Briggs experiment [Vincent, C.L., Briggs, M.J., 1989. Refraction-diffraction of irregular waves over a mound. Journal of Waterway, Port, Coastal and Ocean Engineering, 115(2), pp. 269-284]. The numerical simulations are conducted by constituting two numerical model systems: a combination of SWAN(a wave model) plus SHORECIRC(a current model) and a combination of REF/DIF 1(a wave model) plus SHORECIRC. A time dependent phase-resolving wave-current model, FUNWAVE, is also utilized to simulate the experiment. In the simulations, the breaking-induced currents defocus waves behind the shoal and bring on a wave shadow zone that shows relatively low wave height distributions. The computed results of the two model systems agree better with the measurements than the computed results obtained by neglecting wave-current interaction do. However, it is found that the radiation stresses for standing waves are misevaluated in the wave models. In addition, the results of FUNWAVE show very good agreement with the measurements. The agreement indicates that it is necessary to take into account the effect of breaking-induced current on wave refraction when wave-breaking occurs over a submerged shoal.

• Current Research on Agriculture and Life Sciences
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• v.21
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• pp.39-47
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• 2003

The object of this study was to analyze influence which Black locust afforestation affected to soil, to research change of soil environment of Black locust planted stand and adjacent stands through comparative analyzing physichemical property of soil of them. It was investigated that the soil texture of Black locust planted stand and the adjacent stands was mostly SL. and LS. According to this result I could know that transformation of soil texture by vegetation easily happened because transformation soil texture results from the parent rock. I could ascertain that difference existed between Black locust planted stand and the other stands in soil pH and Carbon content of planted stand of Black locust pH, contents of Available $P_2O_5$ Carbon, Total Nitrogen. In other words, pH and Carbon content of Black locust planted stand were lower than that of Oak forest, higher than that of Pine forest. These results showed that Black locust was which fix nitrogen. I could infer that Black locust afforestation made forest soil fertilized. In soil of Black locust planted stand and the adjacent stands, statistical value appeared highly in pH and existed in content of Carbon, Total nitrogen, too. And high interrelationship appeared in the order of pH, C/N Ratio, CEC, Total nitrogen content was in each stands. I suppose that the trespassing extent of Black locust to Oak forest and Pine forest which are adjacent to planted stand of Black locust is different because of low pH, lackness of Available $P_2O_5$ Carbon, and what not.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.13 no.8
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• pp.804-810
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• 2002

The code division multiple access(CDMA) system capacity is limited by the amount of interference of the system. To reduce the unnecessary interference, this paper proposes optimized cell identification codes for site selection diversity transmission(SSDT) power control in wideband code division multiple access system of third generation partnership project(3GPP). The main objective of SSDT power control is to transmit on the downlink from the primary cell, and thus reducing the interference caused by the multiple transmission. In order to select a primary cell, each cell is assigned a temporary identification(ID) and user equipment(UE) periodically informs a primary cell ID to the connecting cells during soft handover. The non-primary cells selected by UE do not transmit the dedicated physical data channel(DPDCH) to reduce the interference. A major issue with the SSDT technology is the impact of uplink symbol errors on its performance. These errors can corrupt the primary ID code and this may lead to wrong decoding in the base station receivers. The proposed SSDT cell ID codes are designed to minimize the problem and to be easily decoded using simple fast Hadamard transformation(FHT) decoder.

• Archives of Pharmacal Research
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• v.21 no.6
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• pp.657-663
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• 1998

The enzyme responsible for the synthesis of nitric oxide (NO) from L-arginine in mammalian tissues is known as nitric oxide synthase (NOS) (EC.1.14.13.39). In the present study, the role of NO in the regulation of exocrine secretion was investigated in rat pancreatic acinar cells. Treatment of rat pancreatic acinar cells with cholecystokinin-octapeptide (CCK-OP) resulted in an increase in the arginine conversion to citrulline, the amount of $NO_X$, the release of amylase, and the level of CGMP. Especially, CCK-OP-stimulated increase of arginine to citrulline transformation, the amount of $NO_X$, and CGMP level were completely counteracted by the inhibitor of NOS, NG-monomethyl-L-arginine (MMA), by contrast, that of amylase release was partially reduced. Furthermore, MMA-induced decrease of NOS activity and amylase release showed dose-dependent pattern. The data on the time course of CCK-OP-induced citrulline formation and CGMP rise indicate that NOS and guanylate cyclase were activated by treatment of CCK-OP. However, the mechanism of agonist-stimulated guanylate cyclase activation in acinar cells remains unknown. Therefore, activation of NOS is one of the early events in receptor-mediated cascade of reactions in pancreatic acinar cells and NO, not completely, but partially mediate pancreatic enzyme exocrine secretion.

• Journal of Welding and Joining
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• v.32 no.5
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• pp.44-49
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• 2014

The effect of weldbond hybrid joining process on the mechanical behavior of single lap and L-tensile joints was investigated for the newly developed 1.2GPa grade ultra high strength TRIP(transformation induced plasticity) steel. In the case of single lap shear behavior, the weldbond joint of 1.2GPa TRIP steel showed lower maximum tensile load and elongation than that of the adhesive bonding only. It was considered to be due to the reduction of real adhesion area, which was caused by the degradation of adhesive near the spot weld, and the brittle fracture behavior of the spot weld joint. In the case of L-tensile behavior, however, the maximum tensile load of the weldbond joint of 1.2GPa TRIP steel was dramatically increased and the fracture mode was change to the base metal fracture which is desirable for the spot weld joint. These synergic effect of the weldbond hybrid joining process in 1.2GPa TRIP steel was considered to be due to the stress dissipation around the spot weld joint by the presence of adhesive which resulted in the change of crack propagation path.

• Journal of the Korean Ceramic Society
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• v.30 no.6
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• pp.449-456
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• 1993

Sintering behaviour of zirconia powders prepared by different processing treatment was discussed. About >99% densities of theoretical were obtaiend on sintering at 140$0^{\circ}C$ for 2h in case of 300MPa uniaxially cold-pressed compact. But the lower densities were obtained on sintering above this temperature due to abnormal grain growth enabling the tetragonal to monoclinic phase transformation during cooling resulted in microcracks. All kinds of different dried powders exhibited nearly the same shrinkage behaviour with end-point shrinkage between 19 and 20%, and had maximum shrinkage rate (0.99~1.27%/min) around 120$0^{\circ}C$. During whole sintering process densification was mainly governed by grain growth and rearrangement of agglomerates. Heterogeneous abnormal grain growth and abrupt decrease in shrinkage were observed when continuous interagglomerate pore collapsed into isolated pores.

• Journal of Microbiology and Biotechnology
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• v.24 no.7
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• pp.998-1003
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• 2014

Aspartate aminotransferase (AST; E.C. 2.6.1.1), a vitamin B6-dependent enzyme, preferentially promotes the mutual transformation of aspartate and ${\alpha}$-ketoglutarate to oxaloacetate and glutamate. It plays a key role in amino acid metabolism and has been widely recommended as a biomarker of liver and heart damage. Our study aimed to evaluate the extensive preparation of AST and its application in quality control in clinical laboratories. We describe a scheme to express and purify the 6His-AST fusion protein. An optimized sequence coding AST was synthesized and transformed into Escherichia coli BL21 (DE3) strain for protein expression. Ideally, the fusion protein has a volumetric productivity achieving 900 mg/l cultures. After affinity chromatography, the enzyme activity of purified AST reached 150,000 U/L. Commutability assessment between the engineered AST and standard AST from Roche suggested that the engineered AST was the better candidate for the reference material. Moreover, the AST showed high stability during long-term storage at $-20^{\circ}C$. In conclusion, the highly soluble 6His-tagged AST can become a convenient tool for supplying a much better and cheaper standard or reference material for the clinical laboratory.