• Title/Summary/Keyword: K-L transformation

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A Study on the Stimulus Reaction of PBLG (Poly-${\gamma}$-Benzyl $_L$-Glutamate의 자격반응에 관한 연구)

  • Kim, Beyung-Geun;Lee, Kyung-Sup
    • Proceedings of the KIEE Conference
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    • 2002.07c
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    • pp.1413-1415
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    • 2002
  • The Displacement current measurement system used in this experiment because detecting the dynamic behavior of monolayers at the air-water interface is possible. It basically consists of a film balance, a pair of electrodes connected to each other through a sensitive ammeter. Here, one electrode is suspended in air and the other electrode is placed in the water. PBLG phase transformation measured by Maxwell-displacement-current-measurement method in surface of the water. Measured (surfacc pressure, displacement current and dipole moment) of monolayers of PBLG on the water surface. We measured displacement current that occur when changed temperature(15, 20, 25$^{\circ}$ ) and the compression speed(30, 40, 50(mm/min)). From the result, it is known that curren generated in the range of high surface pressur compression velocity and temperature become faste.

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Formal Modeling and Verification of an Enhanced Variant of the IEEE 802.11 CSMA/CA Protocol

  • Hammal, Youcef;Ben-Othman, Jalel;Mokdad, Lynda;Abdelli, Abdelkrim
    • Journal of Communications and Networks
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    • v.16 no.4
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    • pp.385-396
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    • 2014
  • In this paper, we present a formal method for modeling and checking an enhanced version of the carrier sense multiple access with collision avoidance protocol related to the IEEE 802.11 MAC layer, which has been proposed as the standard protocol for wireless local area networks. We deal mainly with the distributed coordination function (DCF) procedure of this protocol throughout a sequence of transformation steps. First, we use the unified modeling language state machines to thoroughly capture the behavior of wireless stations implementing a DCF, and then translate them into the input language of the UPPAAL model checking tool, which is a network of communicating timed automata. Finally, we proceed by checking of some of the safety and liveness properties, such as deadlock-freedom, using this tool.

Studies on Ozonation of Nicotine (니코틴의 오존화 반응에 관한 연구)

  • Taek-Kyu Park;Ki-Hwan Kim;Tae-Sung Huk
    • Journal of the Korean Chemical Society
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    • v.28 no.5
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    • pp.323-327
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    • 1984
  • Nicotine was reacted with an equimolar amount of ozone in methylene chloride and distilled water. The reaction mixture was separated by column chromatography or thin layer chromatography and then purified by vacuum distillation. The six compounds obtained from this reaction were characterized by NMR, IR and Mass spectrometry. These were identified as unchanged nicotine, nornicotine, anabasine, ${\beta}$-nicotyrine, cotinine and nicotine-N-oxide. From these results, reaction mechanism for the ozonolysis ofon nicotine was proposed; the pyrrolidine ring is attacked by ozone at the 1'-position followed by further transformation.

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Selection of Herbicide Resistant Potatoes Transformed with Phosphinothricin Acetyltransferase Gene (Phosphinothricin Acetyltransferase 유전자 도입에 의한 제초제 저항성 감자의 선발)

  • Han, S.S.;Jeong, J.H.;Bang, K.S.;Yang, D.C.
    • Korean Journal of Weed Science
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    • v.17 no.4
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    • pp.390-399
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    • 1997
  • This experiment was conducted to introduce PAT (phosphinothricin acetyltransferase, non-selective herbicide bialaphos resistant gene) gene into potato (Solanum tuberosum. cv. Desiree). Optimal shoot regeneration from leaf discs and stem segments was obtained in MS medium supplemented with 0.1 mg/L IBA and 0.5 mg/L BA, and the frequency of shoot regeneration was 54% in left discs and 46% in stem segments. In this condition, leaf discs and stem segments of potato were co-cultivated with A. tumefaciens MP90 which contained binary vector with GUS: :NPTII gene and PAT gene. Transgenic shoots were regenerated from leaf and stem-derived calli on selection medium with 100mg/L kanamycin. The 100${\mu}M$ acetosyringone treatment during the co-cultivation highly enhanced(4 times than the control) the shoot regeneration on selection medium. When the putative transgenic plants were transferred to medium with 10mg/L basta, all of them were survived. After PCR. GUS test, and Southern blot analysis of the survived plant, we confirmed that the gene was stably integrated into the potato genome and expressed. After the transgenic plants were transplanted in soil, and the transgenic plants were sprayed with the herbicide basta (300ml/10a), the transgenic plants remained green but control plants were died.

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High Frequency Bulblet Formation in Scale and Stem Thin Cell Layer Explant Cultures of Lilium Oriental Hybrids (오리엔탈 백합의 인편 및 줄기의 박판 세포층 절편으로부터 고빈도 자구형성)

  • Oh, Seung-Cheol;Chung, Myung-Hee;Kim, Suk-Won;Liu, Jang-Ryol
    • Journal of Plant Biotechnology
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    • v.30 no.3
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    • pp.251-255
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    • 2003
  • An efficient system for in vitro bulblet formation of Lilium oriental hybrids(cvs). Casa Blanca and Siberia is described. Transverse thin cell layer(tTCL)(1mm thick) explants of 'Casa Blanca' formed bulblets at a frequency of 97.7% when cultured on Murashige and Skoog (MS) medium supplemented with 1mg/L 2,4 dichlorophenoxyacetic acid(2,4-D) (On average 15.6 bulblets were formed per explant). The frequency of bulblet formation was drastically reduced when the explant ghickness was thinner than 1 mm. Explants from the outermost layer of bulb scale produced greater frequency of bulblet formation than middle or innermost layer. Among auxins supplemented to culture medium at 1 mg/L, 2,4-D led to greater frequency of bulblet formation on explants than dicamba, picamba, or phenylacetic acid(PAA). tTCL explants from the middle region of the outermost layer bulb scale yielded greater frequency of bulblet formation than the upper or lower region. tTCL stem explants of 'Siberia' formed bulblets at a frequency of 95.3% when cultured on MS medium with 1 mg/L 2,4-D(On average 9.1 bulblets were formed per explant). The system estabilished in this study will be useful for in vitro rapid propagation and genetic transformation of Lilium Oriental hybrids.

Production of Transgenic Orchardgrass Overexpressing a Thermotolerant Gene, DgP23 (내열성 유전자 DgP23을 도입한 형질전환 오차드그라스의 생산)

  • Kim Ki-Yong;Jang Yo-Soon;Park Geun Je;Choi Gi Jun;Seong Byung Ryul;Seo Sung;Cha Joon-Yung;Son Daeyong
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.25 no.4
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    • pp.267-274
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    • 2005
  • To develop transgenic orchardgrass (Dactylis glomerata L.) resistant to high temperature, a thermptolerance gene, DgP23, was introduced into orchardgrass using Agrobacterium - mediated transformation method. PCR and Southern blot analyses using genomic DNA showed specific DNA band on agarose gel and hybridization signal on X- ray film in transgenic orchardgrass harboring the recombinant DgP23 gene, but not in the wild type and empty vector control plants. RT-PCR and Southern blot analyses using total RNA also showed specific DNA band and hybridization signal. Transgenic orchardgrass did not showed ny morphological aberration both in the green house and field cultivation. Thermotolerance of transgenic plants was not detected in laboratory test. but may detected in field test.

Effects of Medium Supplements on Seed-derived Callus Culture of Italian Ryegrass (배지첨가물질이 이탈리안 라이그래스의 종자유래 캘러스 배양에 미치는 영향)

  • Woo, H.S.;Lee, B.H.
    • Journal of Animal Science and Technology
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    • v.46 no.2
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    • pp.243-250
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    • 2004
  • In an effort to optimize tissue culture responses of Italian ryegrass(Lolium multiflorum Lam.) for future genetic manipulations to improve forage characteristics, the effects of culture medium supplements on tissue culture responses were investigated with mature seeds of three cultivars, 'Jeanne', 'Florida-80' and 'Metro', as explant tissues. For all explants, MS medium containing 5mg/L 2,4-D was optimal for embryogenic callus induction from mature seed and had a strong effect on successive plant regeneration. The optimal concentration of dicamba for the induction of embryogenic callus from mature seeds was 7mg/L. The highest plant regeneration frequency was observed when embryogenic callus was transferred to N6 medium supplemented with 1mg/L 2,4-D and 5mg/L BA. Plant regeneration frequency of callus cultured in the dark was higher than that of cultured in the light. Casein hydrolysate and L-proline improved both in embryogenic callus induction from mature seeds and plant regeneration. High-frequency regeneration system established in this study will be useful for molecular breeding of Italian ryegrass through genetic transformation.

A Spatial Analysis Supporting System Based On CRM And Data Mining Technique

  • Seo, Jeong-Min;Wei, Hu Xiao;Lee, Sang-Moon
    • Journal of Korea Multimedia Society
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    • v.12 no.6
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    • pp.777-784
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    • 2009
  • Recently, the importance of geoCRM (geographic Customer Relationship Management) systems are growing rapidly. So, result of the recognition that their applications extend well beyond the traditional CRM systems with the advent of ubiquitous environment and generalized location based services. A majority of traditional CRM systems are either incapable of managing spatial data or are not user-friendly when doing so. On the other hand, the geoCRM systems can be built as providing the geographic-based functions about CRM, including spatial and market analyses and the visualization of customer data, etc. However, it lacks the specific model and implementation of the geoCRM systems, being caused by the incomprehension of needs, the absence of related standards and the difficulties of development, and so on. In this paper, we develop a new spatial analysis supporting system that to enhance productivity through the convenient use and management of spatial data. The functionality provided by our system includes a set of analysis functions based on data mining techniques which allow a user to affect powerful transformation on spatial data. Particularly, both spatial data and non-spatial attributes can be efficiently handled as an object through our OODBMS.

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A study on the wave control function of ecosystem control structures (생태계제어 구조물의 파랑제어 효과에 관한 연구)

  • 김현주;류청로;손원식
    • Journal of Ocean Engineering and Technology
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    • v.10 no.4
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    • pp.149-159
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    • 1996
  • Multipurpose development of the coast and ocean can be considered as multifunction construction combining the functions of coastal protection, waterfront amenity and creation or rehabilitation of habitats. Multfunction development of coastal and ocean spaces can be accomplished by applying the ecosystem control structure of artificial habitats which will cultivate fishing ground with ecological harmony to the coastal protection system. To evaluate the applicability of ecosystem control structures as as fundamental coastal protection structure, wave control function of the structure is studied by numerical and physical analyses. Dimensional analysis and hydraulic experiment point out the importance of width and crest depth of ecosystem control structure, construction water depth and wave steepness. Wave control efficiency is estimated by the attenuation coefficient $(K_H)$ according to wave steepness $(H_0/L_0)$, relative constructed water depth $(h_i/H_0)$, relative berm width $(B/L_0)$ and relative crest depth $(h_B/H_0)$ of eosystem control structure. Empirical fomulas are suggested based on the results of model test by applying the multiple model based on this experimental results and numerical wave shoaling-dissipation-breaking model appears to be valid for the analysis of wave transformation around ecosystem control structure in the coastal waters.

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Evolution of the Vortex Melting Line with Irradiation Induced Defects

  • Kwok, Wai-Kwong;L. M. Paulius;Christophe Marcenat;R. J. Olsson;G. Karapetrov
    • Progress in Superconductivity
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    • v.3 no.1
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    • pp.5-12
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    • 2001
  • Our experimental research focuses on manipulating pinning deflects to alter the phase diagram of vortex matter, creating new vortex phases. Vortex matter offers a unique opportunity for creating and studying these novel phase transitions through precise control of thermal, pinning and elastic energies. The vortex melting transition in untwinned YB $a_2$C $u_3$ $O_{7-}$ $\delta$/ crystals is investigated in the presence of disorder induced by particle irradiation. We focus on the low disorder regime, where a glassy state and a lattice state can be realized in the same phase diagram. We follow the evolution of the first order vortex melting transition line into a continuous transition line as disorder is increased by irradiation. The transformation is marked by an upward shift in the lower critical point on the melting line. With columnar deflects induced by heavy ion irradiation, we find a second order Bose glass transition line separating the vortex liquid from a Bose glass below the lower critical point. Furthermore, we find an upper threshold of columnar defect concentration beyond which the lower critical point and the first order melting line disappear together. With point deflect clusters induced by proton irradiation, we find evidence for a continuous thermodynamic transition below the lower critical point..

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