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Globotriaosylceramide (Gb3) content in HeLa cells is correlated to Shiga toxin-induced cytotoxicity and Gb3 synthase expression

  • Shin, In-Sun;Ishii, Satoshi;Shin, Jong-Seo;Sung, Kyong-Il;Park, Byung-Sung;Jang, Hyun-Yong;Kim, Byong-Wan
    • BMB Reports
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    • v.42 no.5
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    • pp.310-314
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    • 2009
  • Globotriaosylceramide (Gb3) and globotetraosylceramide (Gb4) are the proposed functional receptors for Shiga toxin (Stx). To elucidate the effect of Gb3 content on Stx-induced cytotoxicity in HeLa cells, we cloned HeLa cells and determined the correlation between glycolipids content and Stx-induced cytotoxicity. The 29 HeLa cell clone (HLCC) lines used showed a wide range of sensitivity to Stx, compared to Gb3-rich cells which were more sensitive, showing as little as 20% viability to 100 pg/ml Stx. In contrast, Gb3-deficient cells proved resistant as they were more than 80% viable to 100 ng/ml Stx. Gb3 content in the HLCC lines corresponded with Stxs-induced cytotoxicity as well as Gb3 synthase expression, but no correlation with Gb4 content was noted. These data show that Gb3 content, which is regulated by the expression of Gb3 synthase, determines the sensitivity of HeLa cells toward Stx.

3D Figure Creation System Based on Content-Awareness for 3D Printing (3D 프린팅을 위한 콘텐츠 인지 기반 3D 개인 피규어 생성 시스템)

  • Lim, Seong-Jae;Hwang, Bon-Woo;Yoon, Seung-Uk;Jeon, Hye-Ryeong;Park, Chang-Joon;Choi, Jin-Sung
    • Journal of the Korea Computer Graphics Society
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    • v.21 no.3
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    • pp.11-16
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    • 2015
  • We present a system for generating 3D personalized figures. This system provides 3D figures model modification and combination functions based on the content-awareness. The integrity of the 3D model must be guaranteed at the time of slicing of the 3D model for 3D printing. In addition to this, with 3D printing, we generally have to print a hollow model in order to save money, time, and the integrity of the print. This paper proposes the automatic algorithm that creates the 3D individual figures with depth sensor and the easy UI functions for deformation, thickness adjustment, and combination of the generated 3D figures model based on the content-awareness. Our proposed method maintains the unique features of the generated 3D figures and ensures the successful 3D printing.

Swelling Pressures of a Potential Buffer Material for High-Level Waste Repository

  • Lee, Jae-Owan;Cho, Won-Jin;Chun, Kwan-Sik
    • Nuclear Engineering and Technology
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    • v.31 no.2
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    • pp.139-150
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    • 1999
  • The swelling pressure of a potential buffer material was measured and the effect of dry density, bentonite content and initial water content on the swelling pressure was investigated to provide the information for the selection of buffer material in a high-level waste repository. Swelling tests were carried out according to Box-Behnken's experimental design. Measured swelling pressures were in the wide range of 0.7 Kg/$\textrm{cm}^2$ to 190.2 Kg/$\textrm{cm}^2$ under given experimental conditions. Based upon the experimental data, a 3-factor polynomial swelling model was suggested to analyze the effect of dry density, bentonite content and initial water content on the swelling pressure The swelling pressure increased with an increase in the dry density and bentonite content, while it decreased with increasing the initial water content and, beyond about 12 wt.% of the initial water content, levelled off to nearly constant value.

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Comparing the Formats and Content of the State Science Content Standards of Six States in U. S. with Emphasis on Earth Science

  • Kim Chan-Jong;Lee Sun-Kyung;Hwang Eunjee
    • Journal of the Korean earth science society
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    • v.26 no.4
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    • pp.336-346
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    • 2005
  • This study identifies and compares science content standards that are approved by departments of education in six states: California, Connecticut, Michigan, New Jersey, Texas, and Virginia. Specifically, the study examines the goals/visions, the organizing/progression principles, the strands of science content, and earth science content found in the states curriculum standards compared to National Science Education Standards. Although many states followed the recommendations of NSES or Project 2061, the format and content of the state science standards reviewed are very diverse. The diversity seems to reflect the diverse perspectives and needs of the states. The results of this study provide Korean educators and teachers with useful models or examples to incorporate Korean national science curriculum guides into the science curriculum frameworks of their regions or schools.

Numerical study on the effect of crack network representation on water content in cracked soil

  • Krisnanto, Sugeng;Rahardjo, Harianto;Leong, Eng Choon
    • Geomechanics and Engineering
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    • v.21 no.6
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    • pp.537-549
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    • 2020
  • The presence of cracks changes the water content pattern during seepage through a cracked soil as compared to that of intact soil. In addition, several different crack networks may form in one soil type. These two factors result in a variation of water contents in the soil matrix part of a cracked soil during seepage. This paper presents an investigation of the effect of crack network representation on the water content of the soil matrix part of cracked soil using numerical models. A new method for the numerical generation of crack networks incorporating connections among crack endpoints was developed as part of the investigation. Numerical analysis results indicated that the difference in the point water content was large, whereas the difference in the average water content was relatively small, indicating the uniqueness of the crack network representation on the average water content of the soil matrix part of cracked soil.

Design of 8K Broadcasting System based on MMT over Heterogeneous Networks

  • Sohn, Yejin;Cho, Minju;Paik, Jongho
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.11 no.8
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    • pp.4077-4091
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    • 2017
  • This paper presents the design of a broadcasting scenario and system for an 8K-resolution content. Due to an 8K content is four times larger than the 4K content in terms of size, many technologies such as content acquisition, video coding, and transmission are required to deal with it. Therefore, high-quality video and audio for 8K (ultra-high definition television) service is not possible to be transmitted only using the current terrestrial broadcasting system. The proposed broadcasting system divides the 8K content into four 4K contents by area, and each area is hierarchically encoded by Scalable High-efficiency Video Coding (SHVC) into three layers: L0, L1, and L2. Every part of the 8K video content divided into areas and hierarchy is independently treated. These parts are transmitted over heterogeneous networks such as digital broadcasting and broadband networks after going through several processes of generating signal messages, encapsulation, and packetization based on MPEG media transport. We propose three methods of generating streams at the sending entity to merge the divided streams into the original content at the receiving entity. First, we design the composition information, which defines the presentation structure for displays. Second, a descriptor for content synchronization is included in the signal message. Finally, we define the rules for generating "packet_id" among the packet header fields and design the transmission scheduler to acquire the divided streams quickly. We implement the 8K broadcasting system by adapting the proposed methods and show that the 8K-resolution contents are stably received and serviced with a low delay.

Changes of asparagine content in potato (Solanum tuberosum L.) tuber during storage (저장 중 감자 아스파라진 함량의 변화)

  • Jin, Yong Ik;Cho, Ji Hong;Chang, Dong Chil;Im, Ju Sung;Park, Young Eun;Yu, Hong Sub;Jeong, Jin Cheol;Park, Kyeong Hun;Chung, Il Min
    • Journal of Plant Biotechnology
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    • v.39 no.4
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    • pp.300-304
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    • 2012
  • In order to research changes of asparagine content according to stage after harvest to storage, it was analysed asparagine content of potato tuber and potato sprout. The results of the asparagine content in potato cultivars after harvest ranked in cv. Superior, cv. Gahwang, cv. Atlantic and cv. Goun in descending order. The content of asparagine in potato was approximately 1300 mg/100g (D.W.) The changes the asparagine content according to storage condition and the difference of asparagine between tuber and sprout can be summarized as follows. In the asparagine content of potato cultivars, Little variation in the content was observed while potato tubers were stored at $4^{\circ}C$. However, after 3 months of storage at $10^{\circ}C$, the content increased by 9.7%, indicating that the content increases as the storage temperature and period increases. The content of asparagine also increased during sprouting of potato tubers. In the sprout, the proximal region contained 21% higher content of asparagines than the distal region, which suggests that the sprout develops with the increasing of the content of asparagines.

A Geochemical Study on the Chindong and Yucbeon - Eonyang Granites in Relation to Mineralization (진동화강암 및 유천-언양화강암의 광화작용에 관한 지화학적 연구)

  • Lee, Jae Yeong
    • Economic and Environmental Geology
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    • v.22 no.1
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    • pp.21-34
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    • 1989
  • Chindong granites are classified into granodiorite, tonalite and quartz-diorite, and Yucheon - Eonyang granites into monzo-granite by the Streckeisen diagram. These granitic rocks of Cretaceous age show trend of calc-alkaline magma, and the magmatic evolution from basic to acidic rocks is consistant with the general crystallization path of the Cretaceous granitic rocks in the Gyeongsang basin. On the basis of petrological and petrochemical data, variation of major elements (K, Na, Ca, Mg) and trace elements (Rb, Sr, Ba) including ore metals (Cu, Pb, Zn) in the Cretaceous granitic rocks were studied in detail in order to investigate geochemical difference of the granitic rocks in relation to mineralization between Cu province and Pb-Zn province in the Gyeongsang basin. There is clear difference in content of the major elements between Chindong granites and Yucheon-Eonyang granites : Chindong granites have low content of K (1.62%) and Na (2.53%), and high content of Ca (3.75%) and Mg (1.42%) whereas Yucheon-Eonyang granites have high content of K (3.56-3.60%), and low content of Ca (0.96-0.26%) and Mg (0.26-0.21%). There is also clear difference in content of trace lithophile elements between Chindong granites and Yucheon-Eonyang, granites : Chindong granites have low content of Rb (86ppm) and Ba (330ppm), and high content of Sr (405ppm) while Yucheon-Eonyang, granites have high content of Rb (144-161ppm) and Ba (983-1030ppm), and low content of Sr (157-136ppm). The lithophile trace elements of Rb and Sr vary with close relationship to major elements of K and Ca, respectively. Therefore, Chindong granites are much easily distinguished from Yucheon-Eonyang granites by using relationship of K with Rb and Ca with Sr : K<3%, Rb<100ppm, Ca<2% and Sr>200ppm for Chindong granites, and K>3%, Rb>100ppm, Ca<2%, and Sr<200ppm for Yucheon-Eonyang granites. There is not clear difference in content of trace ore metals between Chindong granites and Yucheon-Eonyang granites : Chindong granites of the Cu province have low Cu content (15ppm) which is nearly equal to 13-14ppm of Yucheon-Eonyang granites of the Pb-Zn province, and Yucheon-Eonyang granites have Pb content (29-27ppm) which is rather lower than 37ppm of Chindong granites. But Cu is anomalously high in the mineralized part of Chindong granites in Gunbuk-Haman area, and Zn is apparently higher in Yucheon-Eonyang granites (51-37ppm) than in Chindong granites (29ppm). K/Pb ratio is also c1early distinguishable between Chindong granites (<850) and Yucheon-Eonyang granites (>850). Thus, it may be possible to apply geochemical difference of the granites to distinguish whether a Cretaceous granitic body is Cu related rock or Pb-Zn related rock, and whether it belongs to Cu province or Pb-Zn province in the Gyeongsang basin.

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Milled Rice Recovery Rate of Paddy with Various Moisture Contents (벼의 함수율에 따른 도정수율의 변화)

  • 하유신;박경규;김혁주;홍동혁;나규동
    • Journal of Biosystems Engineering
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    • v.27 no.2
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    • pp.125-132
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    • 2002
  • In order to investigate the optimum moisture content of paddy for milling process, a series of tests were conducted by examining the recovery rate and whiteness of milled rice in relation with the various moisture content. Hwabong-byeo and Dongjin-byeo varieties which were major paddies cultivated in Korea were used for the experiment. The test was performed with small experimental milling machines. In order to minimize the unexpected factors, environment conditions were kept in constant during the experiment. As a result, the recovery rate of milled rice were varied as the changes in milling time and degree of whiteness. However, the recovery rate of milled rice increases as its moisture content increases untill a certain point of moisture content and decreases slowly afterward. This certain point can be called optimum moisture content for rice milling. Also, it has a different value depending on the variety. In this experiment, optimum moisture content of Hwabong-byeo and Dongjin-byeo were considered around 14.8% and 15.3%, respectively. It is not sure that these optimum moisture contents for the two varieties would assume the same values irrespective of harvest year and place. However, it could be concluded that the optimum moisture content for rice milling is around 15%(w.b.) for Hwabong-byeo and 15.5%(w.b.) for Dongjin-byeo, respectively.

Effect of cooking methods on the phytosterol content in nine selected vegetables

  • Shin, Jung-Ah;Park, Jong-Min;Lee, Ki-Teak
    • Korean Journal of Agricultural Science
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    • v.43 no.1
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    • pp.87-99
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    • 2016
  • Phytosterol contents in nine vegetables such as paprika (red, yellow, and orange), kohlrabi, bamboo shoot, cherry tomato, cucumber, Chinese chive, and corn were analyzed by gas chromatography. Individual contents of ${\beta}$-sitosterol, campesterol, and stigmasterol in fresh and cooked vegetables (boiling, grilling, stir-frying, deep-frying, steaming, roasting, and microwaving) were determined and compared. Total phytosterol content of paprika, cucumber, Chinese chive and cherry tomato ranged from 23.19 to 46.51 mg/kg (0.002-0.005%) of fresh weight of raw vegetables. Total phytosterol content variation (%) was obtained as follows: [(the content of phytosterol after cooking) - (the content of phytosterol before cooking)] / (the content of phytosterol before cooking) ${\times}100$. Total phytosterol content was found to be high in raw kohlrabi at 138.99 mg/kg fw (0.01%), in corn at 302.86 mg/kg fw (0.03%), and in bamboo shoot at 443.15 mg/kg fw (0.04 %). Total phytosterol content variation (%) in orange paprika ranged from 27.5 to 267.3 while that in cherry tomato ranged from -11.0 to 337.5. Generally, high content variation of total phytosterol was found in stir-fried and deep-fried vegetables. Therefore, higher phytosterol levels were obtained from cooked vegetables than raw vegetables. We suggest that these data will be useful to investigate cooking methods for increased intake of phytosterols.