• 한국발생생물학회지:발생과생식
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• 제17권1호
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• pp.9-16
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• 2013

Coactivator-associated arginine methyltransferase 1 (CARM1) is included in the protein arginine methyltransferase (PRMT) family, which methylates histone arginine residues through posttranslational modification. It has been proposed that CARM1 may up-regulate the expression of pluripotency-related genes through the alteration of the chromatin structure. Mouse embryonic stem cells (mESCs) are pluripotent and have the ability to self-renew. The cells are mainly used to study the genetic function of novel genes, because the cells facilitate the transmission of the manipulated genes into target mice. Since the up-regulated methylation levels of histone arginine residue lead to the maintenance of pluripotency in embryos and stem cells, it may be suggested that CARM1 overexpressing mESCs elevate the expression of pluripotency-related genes in reconstituted embryos for transgenic mice and may resist the differentiation into trophectoderm (TE). We constructed a fusion protein by connecting CARM1 and 7X-arginine (R7). As a cell-penetrating peptide (CPP), can translocate CARM1 protein into mESCs. CPP-CARM1 protein was detected in the nuclei of the mESCs after a treatment of 24 hours. Accordingly, the expression of pluripotency-related genes was up-regulated in CPP-CARM1-treated mESCs. In addition, CPP-CARM1-treated mESC-derived embryoid bodies (EBs) showed an elevated expression of pluripotency-related genes and delayed spontaneous differentiation. This result suggests that the treatment of recombinant CPP-CARM1 protein elevates the expression of pluripotency-related genes of mESCs by epigenetic modification, and this protein-delivery system could be used to modify embryonic fate in reconstituted embryos with mESCs.

• 로봇학회논문지
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• 제13권1호
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• pp.72-78
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• 2018

This paper proposes a Dual Controller System for Precision Control (DCSPC) for control of the gripper. The DCSPC consists of two subsystems, CDSP (Controller based DSP) and CARM (Controller based ARM processor). The CDSP is developed on a DSP processor and controls the gripping motor and LVDT. In particular, the CARM is implemented using Linux and ARM processor according to recent research related to open-source. The robot for high-precision assembly is divided into the robot control and the gripper control section and controls CARM and CDSP systems respectively. In this paper, we also proposed and measured the performance of communication API. As a result, it is expected to recognize improvements in communication between CARM and the robot controller, and will continue to conduct relevant research among other commercial robot controllers.

• 한국가스학회지
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• 제11권4호
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• pp.23-28
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• 2007

정량적 원인분석이 가능한 위험기반검사(KS-RBI) 프로그램을 사용하여 화학설비의 사고 피해범위를 산출하고, 일반적인 정량적 위험성 평가를 수행하는 프로그램인 K-CARM에서 얻은 결과와 비교하였다. 그 결과, KS-RBI 프로그램에 의해 산출한 사고피해 크기는 K-CARM의 결과와 비교적 잘 일치하여 KS-RBI 프로그램이 정량적 위험성 평가에 적극 활용할 수 있을 것으로 기대된다. 또한 사업장에서는 가중평균에 의한 사고 피해영역을 활용함으로써 현실성 있는 피해완화조치와 비상조치계획을 수립할 수 있을 것으로 생각된다.

• 한국균학회지
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• 제32권2호
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• pp.145-147
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• 2004

구름버섯(Coriolus versicolor), 상황버섯(Phellinus linteus) 노루궁뎅이버섯(Hericium erinaceus)의 균사체 배양액의 ethyl acetate 추출물이 돼지와 가금류의 설사 유발 세균류인 E. coli K88, E. coli K99, E. coli 987P와 Salmonella typhimurium 14058 균주에 대하여 항균활성을 보였으며, 특히 구름버섯 추출물은 가장 높은 항균활성을 보였다. 한편, kanamycin과 ampicillin에 대해 내성을 보이는 Staphylococus aureus CARM3230과 E. coli CARM1381 균주에 대해서도 세 가지 버섯 배양 추출물 모두 항균활성을 보였으며, 특히, 구름버섯 추출물은 가장 높은 항균 활성을 보였다. 이는, 이들 버섯 균사체 배양 추출물이 기존 항생제 대체용으로 가축 사료에 첨가제로 사용될 수 있다는 가능성을 보였다. 또한 이들 버섯류로부터 항생제 내성균을 억제할 수 있는 신규 항균성 물질의 탐색을 위한 기초자료로서 활용될 수 있을 것이다.

• BMB Reports
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• 제39권3호
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• pp.304-310
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• 2006

Transcription factor NF-E2-related factor 2 (Nrf2) regulates the induction of Phase II detoxifying enzymes and antioxidant enzymes in response to many cancer chemopreventive compounds. In this study, we investigated the role of receptor associated coactivator (RAC3) or steroid receptor coactivator-3 (SRC3) and other nuclear co-regulators including CBP/p300 (CREB-binding protein), CARM1 (Coactivator-associated arginine methyltransferase), PRMT1 (Protein arginine methyl-transferase 1), and p/CAF (p300/CBP-associated factor) in the transcriptional activation of a chimeric Gal4-Nrf2-Luciferase system containing the transactivation domain (TAD) of Nrf2 in HepG2 cells. The results indicated that RAC3 up-regulated the transactivation activity of Gal4-Nrf2-(1-370) in a dose-dependent manner. The enhancement of transactivation domain activity of Gal4-Nrf2-(1-370) by RAC3 was dampened in the presence of dominant negative mutants of RAC3. Next we studied the effects of other nuclear co-regulators including CBP/p300, CARM1, PRMT1 and p/CAF, and the results showed that they had different level of positive effects on this transactivation domain activity of Gal4-Nrf2-(1-370). But importantly, synergistic effects of these co-regulators in the presence of RAC3/SRC3 on the transactivation activity of Gal4-Nrf2-(1-370) were observed. In summary, our present study showed for the first time that the 160 RAC3/SRC3 is involved in the functional transactivation of TAD of Nrf2 and that the other nuclear co-regulators such as CBP/p300, CARM1, PRMT1 and p/CAF can also transcriptionally activate this TAD of Nrf2 and that they could further enhance the transactivation activity mediated by RAC3/SRC3.

• 한국가스학회지
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• 제16권4호
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• pp.38-43
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• 2012

이 논문은 2008년 5월 H석유화학에서 발생한 염화수소 누출사고에 대한 염화수소 누출량 및 피해범위를 정량적으로 산정하고 예방대책을 제시하는데 그 목적이 있다. 염화수소 Column의 안전밸브를 통해 누출된 양은 안전밸브 배출용량, 이상상태방정식 및 기계적 에너지 수지 식을 사용하여 계산한 결과, 최소한 76.8 kg의 염화수소가 누출된 것을 알 수 있었다. 또한, PHAST 등의 프로그램을 활용하여 안전밸브 설치 지점(높이 24 m)으로 부터 약 350 m 떨어진 곳에서의 염화수소 농도를 예측한 결과, 지표면 누출로 가정하여 계산하는 ALOHA 및 K-CARM 프로그램에서는 각각 304 ppm과 1,700 ppm로 예측되었고 누출 높이에서의 지표면 값을 계산하는 PHAST 프로그램에서는 1 ppm 이하로 예측되었다. 위 결과는, 사고당시 염화수소가 안전밸브를 통해 최소 76.8 kg이 누출되었고 누출지점으로부터 약 350 m 떨어진 곳에 있던 근로자들이 1 ppm 이하의 농도의 HCl 가스에 폭로되었음을 말해준다. 또한, 이러한 사고를 예방하기 위해서는 염화수소와 같은 독성물질은 스크러버 등을 거쳐 세정 후 안전하게 대기로 배출(방출) 시켜야 한다는 사실을 제시한다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제32권6호
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• pp.567-572
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• 2010

Intraoperative breakage of instruments can be occurred unexpectedly. To prevent damage of neighboring important anatomic structures and consequent complications, broken instruments should be removed as soon as possible. There have been several methods to remove broken instruments. One of them is the Carm fluoroscopy which is commonly used for locating metal foreign bodies. However, its application for removal of broken instruments in the oral and maxillofacial area is not common. In our experiences with the removal of two broken instruments in mandibular area, the newly developed dental mini C-arm was used to find broken instrument in soft tissue, because it gives real-time in situ information for the intraoperative location. We report two cases with broken instruments, a broken dental needle in the pterygomandibular space and a broken straight bur in the mandibular angle area. They were identified and could be removed safely using a dental mini C-arm.