• Title/Summary/Keyword: K-C

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Identification of Curvularia spp. Isolated from Gramineous Plants in Korea (화본과식물에서 분리한 Curvularia spp.의 동정)

  • Yi, Jeong-Hye;Kim, Jin-Won;Lee, Du-Hyung;Shim, Gyu-Yul
    • The Korean Journal of Mycology
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    • v.30 no.1
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    • pp.56-60
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    • 2002
  • Sixteen Curvularia isolates were collected from leaf spots and blights on gramineous plants of field crops, grass pasture plants, turfgrasses and wild weeds in Korea in 1998. These isolates were identified as C. cymbopogonis, C. inaequalis, C. intermedia, C. lunata, C. lunata var. aeria, C. ovoidea, C. pallescens and C. senegalensis based on the mycological characteristics. Of these, C. cymbopogonis, C. lunata var. aeria, C. ovoidea and C. senegalensis were recorded for the first time in Korea.

Involvement of Crosstalk Between cAMP and cGMP in Synaptic Plasticity in the Substantia Gelatinosa Neurons

  • Kim, Tae-Hyung;Chung, Ge-Hoon;Park, Seok-Beom;Chey, Won-Young;Jun, Sung-Jun;Kim, Joong-Soo;Oh, Seog-Bae
    • International Journal of Oral Biology
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    • v.36 no.2
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    • pp.83-89
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    • 2011
  • Substantia gelatinosa (SG) neurons receive synaptic inputs from primary afferent $A{\delta}$- and C-fibers, where nociceptive information is integrated and modulated by numerous neurotransmitters or neuromodulators. A number of studies were dedicated to the molecular mechanism underlying the modulation of excitability or synaptic plasticity in SG neurons and revealed that second messengers, such as cAMP and cGMP, play an important role. Recently, cAMP and cGMP were shown to downregulate each other in heart muscle cells. However, involvement of the crosstalk between cAMP and cGMP in neurons is yet to be addressed. Therefore, we investigated whether interaction between cAMP and cGMP modulates synaptic plasticity in SG neurons using slice patchclamp recording from rats. Synaptic activity was measured by excitatory post-synaptic currents (EPSCs) elicited by stimulation onto dorsal root entry zone. Application of 1 mM of 8-bromoadenosine 3,5-cyclic monophosphate (8-Br-cAMP) or 8-bromoguanosine 3,5-cyclic monophosphate (8-Br-cGMP) for 15 minutes increased EPSCs, which were maintained for 30 minutes. However, simultaneous application of 8-BrcAMP and 8-Br-cGMP failed to increase EPSCs, which suggested antagonistic cross-talk between two second messengers. Application of 3-isobutyl-1-methylxanthine (IBMX) that prevents degradation of cAMP and cGMP by blocking phosphodiesterase (PDE) increased EPSCs. Co-application of cAMP/cGMP along with IBMX induced additional increase in EPSCs. These results suggest that second messengers, cAMP and cGMP, might contribute to development of chronic pain through the mutual regulation of the signal transduction.

Classification of Cordyceps Species Based on Protein Banding Pattern (단백질 분석을 기초로한 Cordyceps속 동충하초의 분류)

  • Sung, Jae-Mo;Lee, Hyun-Kyung;Yoo, Young-Jin;Choi, Young-Sang;Kim, Sang-Hee;Kim, Yong-Ook;Sung, Gi-Ho
    • The Korean Journal of Mycology
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    • v.26 no.1 s.84
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    • pp.1-7
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    • 1998
  • In order to find relationship within and between entomopathogenic species, analysis of protein band pattern in mycelia of 25 isolates was conducted by UPGMA. The results allowed differentiation of three groups on 85% similarity coefficient. Similarity coefficient within C. militaris was $0.787{\sim}1.000$, C. kyushuensis was 0.958-1.000 and C. pruinosa was 0.993-1.000. C210 and C298 isolates which had somewhat immersed perithecia, comparable to other C. militaris isolates, had 91% similarity. C108, C225-1 and C228 isolates pathogenic on Lepidopterous larvae had 89% similarity. Closely related species to C. militaris were C. kyushuensis and C. pruinosa. And similarity between C. pruinosa and C. kyushuensis was 88%. Similarity between C. bifusispora formed conidia on media and Paecilomyces tenuipes was 89%. C. scarabaeicola pathogenic specifically on adult Scarabaeidae had 82% similarity with above two species. C118 identified as C. militaris showed different protein banding patterns.

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Mechanical and Electrical Properties of Si-SiC Fabricated Using SiC-C Composite Powders Synthesized by Sol-gel Process (Sol-gel 법으로 합성된 SiC-C 복합분말을 사용하여 제조된 Si-SiC의 기계적 특성 및 전기저항 특성)

  • Youn, Sung Il;Cho, Gyung Sun;Youm, Mi Rae;Lim, Dae Soon;Park, Sang Whan
    • Journal of the Korean Ceramic Society
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    • v.51 no.5
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    • pp.459-465
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    • 2014
  • In this study, Si-SiC composites were fabricated using a Si melt infiltration method using ${\beta}$-SiC/C composite powders synthesized by the carbothermal reduction of $SiO_2-C$ precursors made from a TEOS and a phenol resin. The purity of the synthesized SiC-C composite powders was higher than 99.9993 wt% and the average particle size varied from 4 to $6{\mu}m$ with increasing carbon contents of the $SiO_2-C$ precursors. It was found that the Si-SiC composites fabricated in this study consist of ${\beta}$-SiC and residual Si, without any trace of ${\alpha}$-SiC. The 3-point bending strengths of the fabricated Si-SiC composites were measured and found to be higher than 550 MPa, although the density of the fabricated Si-SiC composite was less than $2.9g/cm^3$. The bending strengths and the densities of the fabricated Si-SiC composites were found to decrease with increasing C/Si mole ratios in the SiC-C composite powders. The specific resistivities of the Si-SiC composites fabricated using the SiC-C composite powders were less than $0.018{\Omega}cm$. With increasing C content in the SiC-C composite powders used for the fabrication of Si-SiC composites, the specific resistivity of the Si-SiC composites was found to slightly increase from 0.0157 to $0.018{\Omega}cm$.

Evaluation of Distribution Characteristics for Petroleum Hydrocarbon in Groundwater by TPH Fraction Analysis (석유계 총 탄화수소(Total Petroleum Hydrocarbons, TPH) 분획분석법을 이용한 지하수 중 유류오염물질 분포특성 평가)

  • Kim, Deok Hyun;Park, Sunhwa;Choi, Min-Young;Kim, Moonsu;Yoon, Jong Hyun;Lee, Gyeong-Mi;Jeon, Sang-Ho;Song, Dahee;Kim, Young;Chung, Hyen Mi;Kim, Hyun-Koo
    • Journal of Soil and Groundwater Environment
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    • v.23 no.5
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    • pp.26-36
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    • 2018
  • Total petroleum hydrocarbon (TPH) is a mixture of various oil substances composed of alkane, alkene, cycloalkane, and aromatic hydrocarbons (benzene, toluene, ethylbenzene, xylene, etc.). In this study, we investigated 92 groundwater wells around 36 gas stations to evaluate distribution characteristics of petroleum hydrocarbons. Groundwater in the wells was sampled and monitored twice a year. The fraction analysis method of TPH was developed based on TNRCC 1006. The test results indicated aliphatic and aromatic fractions accounted for 28.6 and 73.8%, respectively. The detection frequencies of TPH in the monitoring wells ranged in 21.6 - 24.2%. The average concentration of TPH was 0.11 mg/L with the concentration range of 0.25~0.99 mg/L. In the result of TPH fraction analysis, in aliphatic fractions were 19% (C6-C8 : 0.2%, C8-C10 : 0.4%, C10-C12 : 0.4%, C12-C16 : 0.5%, C16-C22 : 1.0%, C22-C36 : 16.6%), and aromatic fractions were 81% (C6-C8 : 1.1%, C8-C10 : 0%, C10-C12 : 2.9%, C12-C16 : 0.3%, C16-C22 : 4%, C22-C36 : 66.8%). Fractions of C22-C36 were detected in about 83% of the monitoring wells, suggesting non-degradable characteristics of hydrocarbons with high carbon content.

Comparison of Pyrolysis Patterns of Different Tobacco Leaves by Double-Shot Pyrolysis-GC/MSD Method

  • Lee, Chang-Gook;Lee, Jae-Gon;Jang, Hee-Jin;Kwon, Young-Ju;Lee, Jang-Mi;Kwag, Jae-Jin;Kim, Soo-Ho;Sung, Yong-Joo;Shin, Chang-Ho;Kim, Kun-Soo;Rhee, Moon-Soo
    • Journal of the Korean Society of Tobacco Science
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    • v.30 no.2
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    • pp.94-102
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    • 2008
  • In this paper, we describe our study on the characterization of tobacco leaves by their pyrolysis patterns. Two kinds of tobacco leaves were pyrolyzed and analyzed by Double-Shot Pyrolysis-Gas Chromatography/Mass Spectroscopy (Py-GC/MS) methods. Three grades of Korean flue-cured tobacco leafsuch as B1O, AB3O, CD3L and burley tobacco leaves such as B1T, AB3T, CD3W were pyrolyzed with six discrete but stepwise heating temperature ranges, those are from 100$^{\circ}C$ to 150$^{\circ}C$, 150$^{\circ}C$ to 200$^{\circ}C$, 200$^{\circ}C$ to 250$^{\circ}C$, 250$^{\circ}C$ to 300$^{\circ}C$, 300$^{\circ}C$ to 350$^{\circ}C$ and finally from 350$^{\circ}C$ to 400$^{\circ}C$. Using the resultant 52 pyrolytic components identified in the programs as components, principal component analysis (PCA) showed statistical classification between flue-cured and burley tobacco lamina. Among six pyrolysis temperature ranges, the best discrimination was achieved at the temperature range from 250$^{\circ}C$ to 300$^{\circ}C$ and from 300$^{\circ}C$ to 350$^{\circ}C$.

Macroscopic Wear Behavior of C/C and C/C-SiC Composites Coated with Hafnium Carbide

  • Lee, Kee Sung;Sihn, Ihn Cheol;Lim, Byung-Joo;Lim, Kwang Hyun
    • Journal of the Korean Ceramic Society
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    • v.52 no.6
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    • pp.429-434
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    • 2015
  • This study investigates the macroscopic wear behaviors of C/C and C/C-SiC composites coated with hafnium carbide (HfC). To improve the wear resistance of C/C composites, low-pressure chemical vapor deposition (LPCVD) was used to obtain HfC coating. The CVD coatings were deposited at various deposition temperatures of 1300, 1400, and $1500^{\circ}C$. The effect of the substrate material (the C/C substrate, the C/C-CVR substrate, or the C/C-SiC substrate deposited by LSI) was also studied to improve the wear resistance. The experiment used the ball-on-disk method, with a tungsten carbide (WC) ball utilized as an indenter to evaluate the wear behavior. The HfC coatings were found to effectively improve the wear resistance of C/C and C/C-SiC composites, compared with the case of a non-coated C/C composite. The former showed lower friction coefficients and almost no wear loss during the wear test because of the presence of hard coatings. The wear scar width was relatively narrower for the C/C and C/C-SiC composites with hafnium coatings. Wear behavior was found to critically depend on the deposition temperature and the material. Thus, the HfC-coated C/C-SiC composites fabricated at deposition temperatures of $1500^{\circ}C$ showed the best wear resistance, a lower friction coefficient, and almost no loss during the wear test.

Effects of Cynanchum spp. on the hyperlipidemia in rats induced by Triton WR-1339 (국산 및 중국산 백수오(白首烏)의 Triton WR-1339 유발 고지혈증(高脂血症) 흰쥐에 대한 효능 연구)

  • Choi, Ho-Young;Ham, In-Hye;Lee, Ju-Yeong;Yoon, Ye-Jin;Yang, Gab-Sik;Tae, Jin;Bu, Young-Min;Kim, Ho-Cheol
    • The Korea Journal of Herbology
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    • v.22 no.4
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    • pp.279-286
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    • 2007
  • Objective : In order to compare of hyperlipidemia effect of C. wilfordii, C. auriculatum in korea and C. auriculatum in China, it was performed on the hyperlipidemia in rats induced by Triton WR-1339. Method : The 70% MeOH extracts from dried root of C. wilfordii and C. auriculatum in korea and C. auriculatum in china were fractionated into H2O layer and CHCl3 layer. The level of total cholesterol, triglyceride, HDL-cholesterol, LDL-cholesterol, AST and ALT in serum were used to observe the effectiveness of C. wilfordii korean C. auriculatum and chinese C. auriculatum. Results : In the hyperlipidemic rats induced by Triton WR-1339, C. wilfordii and korean C. auriculatum significantly decreased total cholesterol, triglyceride, otherwise significantly increased HDL. And C. wilfordii and chinese C. auriculatum significantly decreased AST and ALT. Conclusion : This study indicate that C. wilfordii korean C. auriculatum and chinese C. auriculatum were effective in the hyperlipidemic rats induced by Triton WR-1339. According to the results, C. wilfordii korean C. auriculatum and chinese C. auriculatum need to considered for the application in hyperlipidemia.

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Molecular Phylogenetic Analysis of HIV -1 vif Gene from Korean Isolates

  • Park, Chan-Seung;Kim, Mi-Sook;Lee, Sung-Duk;Kim, Sung-Soo;Lee, Keon-Myung;Lee, Chan-Hee
    • Journal of Microbiology
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    • v.44 no.6
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    • pp.655-659
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    • 2006
  • Phylogenetic studies of nef, pol, and env gene sequences of HIV-1 isolated from Koreans suggested the presence of a Korean clade in which Korean sequences are clustered to the exclusion of foreign sequences. We attempted to identify and characterize the Korean clade using all vif gene sequences isolated from Koreans registered in the NCBI GenBank database (n = 233). Most (77 %) of the Korean isolates belonged to the Korean clade as a large subcluster in subtype B, designated the Korean clade subtype B ($K_{C}B$). $K_{C}B$ sequences were relatively homogenous compared to Korean subtype B sequences that did not belong to the $K_{C}B$ (non-Korean clade subtype B; $NK_{C}B$). Comparison of amino acid frequencies of $K_{C}B$ and $NK_{C}B$ sequences revealed several positions where the amino acid frequencies were significantly different. These amino acid residues were critical in separating $K_{C}B$ from $NK_{C}B$ or from foreign sequences, since substitution of these amino acids in $K_{C}B$ with the $NK_{C}B$ amino acids relocated the $K_{C}B$ sequences to $NK_{C}B$, and vice versa. Further analyses of $K_{C}B$ will help us to understand the origin and evolutionary history of $K_{C}B$.

Unity in HIV-1 Sequence Diversity: Identification and Characterization of Korean Clade in HIV-1 Isolated from Korean

  • Lee, Chan-Hee
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2006.05a
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    • pp.129-131
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    • 2006
  • Through molecular phylogenetic analysis using the nef gene sequences of HIV-l isolated from Korean registered in the NCBI GenBank together with 41 reference strains and 94 foreign isolates, we verified that most (${\sim}80%$) of Korean isolates belonged to subtype B and 78% of subtype B were clustered together exclusively of foreign isolates, and this cluster was named Korean clade subtype B ($K_cB$). Similarity study suggested that the $K_cB$ cluster was more homogeneous than and clearly distinctive from the non-Korean subtype B ($NK_cB$). Comparison of the consensus amino acid sequences of the $K_cB\;or\;NK_cB$ revealed characteristic $K_cB$ signature amino acid pattern comprised of 13 amino acid residues. The $K_cB$ signature amino acid residues were critical in separating the $K_cB$ ftom the $NK_cB$, since substitution of the $NK_cB$ sequences with $K_cB$ signature amino acids relocated them to the Koran clade, and vice versa. Synonymous and nonsynonymous substitution rate study suggested positive selection event for the $K_cB$.

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