• Natural Product Sciences
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• v.10 no.3
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• pp.124-128
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• 2004

Previously, $(1R,9S)-{\beta}-Hydrastine$ hydrochloride has been found to lower dopamine content in PC12 cells (Kim et al., 20001). In this study, the effects of $(1R,9S)-{\beta}-Hydrastine$ hydrochloride on L-DOPA-induced cytotoxicity in PC12 cells were investigated. Treatment with $(1R,9S)-{\beta}-Hydrastine$ hydrochloride at concentrations higher than $500\;{\mu}M$ caused cytotoxicity in PC12 cells. In addition, $(1R,9S)-{\beta}-Hydrastine$ hydrochloride at non-cytotoxic or cytotoxic concentrations significantly enhanced L-DOPA-induced cytotoxicity (L-DOPA concentration, $50\;{\mu}M$). Treatment of PC12 cells with $750\;{\mu}M$ $-1R,9S)-{\beta}-Hydrastine$ hydrochloride and $50\;{\mu}M$ L-DOPA, alone or in combination, also induced cell death via a mechanism which exhibited morphological and biochemical characteristics of apoptosis, including chromatin condensation and membrane blebbing. Exposure of PC12 cells to $(1R,9S)-{\beta}-Hydrastine$ hydrochloride, L-DOPA and $(1R,9S)-{\beta}-Hydrastine$ hydrochloride plus L-DOPA for 48 h resulted in a marked increase in the cell loss and percentage of apoptotic cells compared with exposure for 24 h. These data indicate that $(1R,9S)-{\beta}-Hydrastine$hydrochloride at higher concentration ranges aggravates L-DOPA-induced neurotoxicity cytotoxicity in PC12 cells. Therefore, it is proposed that the long-term L-DOPA therapeutic patients with $(1R,9S)-{\beta}-Hydrastine$ hydrochloride could be checked for the adverse symptoms.

• Journal of Periodontal and Implant Science
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• v.35 no.3
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• pp.675-685
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• 2005

Bone resorption involves sequential stages of osteoclast precursor migration and differentiation of osteoclast precursors into multinucleated osteoclasts. Stromal cell derived factor (SDF)-1 is a chemotactic factor for osteoclast precursor migration. Matrix metalloproteinase (MMP)-9 is involved in migration of osteoclast precursors and activation of $interleukin(IL)-1{\beta}$. Alveolar bone destruction is a characteristic feature of periodontal disease. Treponema lecithinolyticum is a oral spirochete isolated from the periodontal lesions. The effect of lipopolysaccharide(LPS) from T. lecithinolyticum on expression of SDF-1 and MMP-9 was examined in cocultures of bone marrow cells and osteblasts derived from mouse calvariae. T. lecithinolyticum LPS increased expression of MMP-9 in the coculture. Polymyxin B, an inhibitor of LPS, abolished the increase of MMP-9 mRNA expression by LPS. LPS did not increase the expression of SDF-1, $IL-1{\beta}$ and tumor necrosis $factor(TNF)-{\alpha}$ mRNA in cocultures. Prostaglandin $E_2(PGE_2)$ up-regulated the expression of MMP-9 and NS398, an inhibitor of $PGE_2$ synthesis, down-regulated the induction of MMP-9 expression by T. lecitbinolyticm LPS. These results suggest that T. lecitbinolyticm LPS increases MMP-9 expression in bone cells via $PGE_2$ and that the induction of MMP-9 expression by T. lecitbinolyticm LPS is involved in alveolar bone destruction of periodontitis patients by the increase of osteoclast precursor migration and the activation of bone resorption-inducing cytokine.

• Korean Journal of Materials Research
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• v.9 no.11
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• pp.1137-1143
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• 1999

Microstructure and mechanical properties of Mod.9Cr-1Mo and W added 9Cr-0.5Mo2W steels were investigated for liquid metal reactor (LMR) heat exchange tube. The tempering temperatures at which cell structure was formed were $700^{\circ}C$ for Mod.9Cr-1Mo steel and $750^{\circ}C$ for W added 9Cr0.5Mo-2W steel. indicating the recovery of dislocation was delayed by the addition of W. 9Cr-0.5Mo-2W steel had the same kinds of precipitates with Mod.9Cr-1Mo steel, but the W was included in the precipitates in 9Cr-0.5Mo-2W steel. Micro-hardness and ultimate tensile strength of 9Cr-0.5Mo-2W steel were higher than those of Mod.9Cr-1Mo steel. The impact property of Mod.9Cr-1Mo steel was superior to that of 9Cr-0.5Mo-2W steel.

• 전기의세계
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• v.46 no.7
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• pp.25-27
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• 1997

우리나라 배전전압은 1917년 경성전기주식회사가 표준 배전전압으로 3.3kV를 채택한 이래 1960년대 중반까지는 3.3kV, 5.7kV-y, 6.6kV이었다. 1965년 10월, 경북 칠곡군 약목변전소 관내의 당시 박정희 대통령 생가 인근을 통과하는 22kV 비접지 송전선로 9km에 다중접지된 중성선을 추가시설, 3상 4선식 배전선로화하여 4개부락 40kV의 부하를 공급함으로써 우리나라에 22.9kV-y 중성선 다중접지 배전방식 도입의 효시가 된 이래, '22.9kV-y승압'사업은 1970년부터 1986년까지 한전의 배전분야 최우선사업으로 전국 방방곡곡에 걸쳐 시행되었다. 이제는 서울 일부와 제주도에서만 시행중으로 종료단계인 오늘에 이르기까지, '22.9kV-y승압'은 한전의 본사와 전국의 사업장에서 수많은 배전분야 종사자들이 총력을 기울여 이룩한 세계 전력 산업사에 그 유례가 드문 격상에 의한 배전전압 단일화 사업으로써, 배전사업 현대화의 중심사업이었다 하지 않을 수 없다.

• The Korean Journal of Zoology
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• v.30 no.3
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• pp.261-271
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• 1987

계배 중추신경계의 분화에 따른 단백질 합성 양상을 SDS-PAGE와 2차원 전기영동으로 분석하였다. 약 24∼26개의 band가 densitomete떼 의해 기록되었고, 주요 band들의 분자량은 90K에서 lIft 범위에 분포하였다. 대뇌, 시엽 및 소뇌에서 가장 현저한 band는 분자량이 44.7K로 actin으로 사료되었다. 대뇌의 85.5광 및 47.9K, 시엽의 85.5K, 71.6K 및 34.9K 소뇌의 47.9K 및 34.9K band들은 부화전까지 는 증가하다가 부화후 감소하였다. 기록된 약 300개의 polypeptide중 약 9%가 분화가 진행됨에 따라 그들의 합성율에 변화를 보였으며, 대뇌의 spot 19(Mr, 63K; pl, 6.95)와 소뇌의 spot 22(Mr, 43K; pI, 6.5) 는 부화후 4일배에서 새로이 합성되었다.

• Journal of Life Science
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• v.20 no.2
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• pp.275-280
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• 2010

Sulforaphane is a naturally occurring member of the iosothiocyanate family, which reveals chemopreventive capacities including anti-cancer, anti-inflammation and inhibition of MMP-9 activities. In this study, we investigated the effect of sulforaphane on the expression of matrix metalloproteinase-9 (MMP-9) in lipopolysaccharide (LPS)-induced Raw 264.7 cells. Sulforaphane strikingly suppressed the LPS-induced MMP-9 activity and mRNA expression in a dose-dependent manner. In addition, sulforaphane inhibited not only the LPS-induced MMP-9 promoter activity but also LPS-mediated activator protein-1 (AP-1) and nuclear factor-kB (NF-${\kappa}B$) promoter activity. Transient transfection by MMP-9 constructs, in which specific transcriptional factors were mutagenized, indicated that the effects of LPS and sulforaphane were mediated via AP-1 and NF-${\kappa}B$ response elements. We found that sulforaphane had the ability to suppress LPS-induced invasion in vitro. Taken together, these results demonstrated that sulforaphane effectively suppressed LPS-induced MMP-9 expression via modulation of promoter elements (AP-1 and NF-${\kappa}B$) in MMP-9 transcriptional activation.

• The Korean Journal of Physiology and Pharmacology
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• v.15 no.4
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• pp.211-216
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• 2011

Glioblastoma multiforme is one of the most common and aggressive tumors in central nervous system. It often possesses characteristic necrotic lesions with hemorrhages, which increase the chances of exposure to thrombin. Thrombin has been known as a regulator of MMP-9 expression and cancer cell migration. However, the effects of thrombin on glioma cells have not been clearly understood. In the present study, influences of thrombin on glioma cell migration were examined using Boyden chamber migration assay and thrombin-induced changes in MMP-9 expression were measured using zymography, semi-quantitative RT-PCR, and Western blotting. Furthermore, underlying signaling pathways by which thrombin induces MMP-9 expression were examined. Thrombin-induced migration and MMP-9 expression were significantly potentiated in the presence of wortmannin, a PI3K inhibitor, whereas MAPK inhibitors suppressed thrombin-induced migration and MMP-9 expression in C6 glioma cells. The present data strongly demonstrate that MAPK and PI3K pathways evidently regulate thrombin-induced migration and MMP-9 expression of C6 glioma cells. Therefore, the control of these pathways might be a beneficial therapeutic strategy for treatment of invasive glioblastoma multiforme.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.17 no.1
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• pp.1-8
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• 1984

The author carried out an experiment to determine the resistance of trawl net aboard M/S Saebada, training ship of National Fisheries University of Pusan, 2,275 G/T and 3,600ps. Total tension loaded on warp were measured by the recording tension meter. Resistance of the net is estimated by subtracting the resistance of otter boards and warps from the total tension. Coefficient k and exponent n of the formula on the trawl net deduced by Koyama, $R_N=k\frac{d}{l}abv^n$ were calculated from the resistance of the net obtained. The results obtained are can be summarized as follows : 1. Six seamed net with two net pendant k=11, n=1.8 2. Eight seamed net with three net pendant k=11, n=1.8 3. Ten seamed net with three net pendant k=9, n=1.9 4. Ten seamed net with four net pendant k=9, n=1.9

• Archives of Pharmacal Research
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• v.12 no.1
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• pp.5-7
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• 1989

A series of 1-chloro-4-methyl-6-arylazo-9H-thioxanthen-9-ones were synthesized and schistosomicidal activity evaluated. It was found that, 1-chloro-4-methyl-6-(2-hydroxy-1-naphthylazo)-9H-thioxanthen-9-one possessed a promising activity against Schisto-soma mansoni in mice.

• Journal of the Korean Society of Tobacco Science
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• v.9 no.1
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• pp.57-64
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• 1987

The pyrolysis of geometrical isomers of 9-octadecenoic acid were conducted at $600^{\circ}C$ under nitrogen, and its pyrolytic behaviors were investigated. The pyrolytic products of both fatty acids, cis-9-octadecenoic acid(oleic acid) and trans-9-octadecenoic acid(elaidic acid), were analyzed using a gas chromatograph and GC/MS spectrometer. Twenty-seven different compounds were identified from these pyrolyzates, and the chief products of them were ketones, alcohols and aromatic hydrocarbons. And also, the pyrolytic mechanisms of two geometrical isomers of this fatty acid were discussed by a comparison of experimental results.