• Journal of Microbiology and Biotechnology
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• 제11권2호
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• pp.234-241
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• 2001

The C-terminal 393 bp region of the human interleukin-16 (IL-16) gene was cloned and expressed in E. coli along with mammalian cell lines. Recombinant IL-16 expressed from E. coli was 22 kDa on SDS-PAGE and showed 260% of chemoattractant activity at a concentration of $0.1\;{\mu}g/ml$. HeLa, COS, and Neuro-2a cells were transduced by recombinant retrovirus vector pLNC/IL-16/IRES/TK and the intracellular and secreted amounts of IL-16 produced by HeLa/IL-16/TK, COS/IL-16/TK, and Neuro-2a/IL-16/TK cells were determined by enzyme-linked immunosorbent assay (ELISA). HeLa/IL-16/TK $(1{\times}10^5)$ and COS/IL-16/TK $(1{\times}10^5)$ cells secreted 36.1 and 13.3 ng of IL-16 for 48 h, respectively. Forty-nine ng and 86.4 ng of IL-16 remained in the cell lysates of HeLa/IL-16/TK and COS/IL-16/TK. Intracellular and secreted amounts of IL-16 from Neuro-2a/IL-16/TK $(5{\times}10^5)$ cells during 24 h cultivation were 50 ng and 3.3 ng, respectively. Also, HeLa and COS cells wee stably transfected with mammalian expression vector pCRIII/IL-16. Both culture media and cell lysates prepared from HeLa/IL-16 cells and COS/IL-16 cells showed chemoattractant activity ranging from 190% to 460% as compared to the control experiment. Expression of the herpes simplex virus thymidine kinase (HSV0tk) gene in pLNC/IL-16/ IRES/TK bicistronic retroviral expression vector was verified by performing a genciclovir (GCV) sensitivity assay. Finally, IL-16 repressed Tat-transactivated human immunodeficiency virus type 1 long terminal repeat (HIV-1 LTR) promoter activity.

• Genomics & Informatics
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• 제1권2호
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• pp.101-107
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• 2003

Loss of heterozygosity (LOH) has been used to detect deleted regions of a specific chromosome in cancer cells. LOH on chromosome 16q has been reported to occur frequently in progressed hepatocellular carcinoma (HCC). Liver tissues from 37 Korean HCC patients were analyzed for LOH by using 25 polymorphic microsatellite markers distributed along 16q. Out of the 37 HCC patients studied, 21 patients (56.8%) showed LOH in various regions of 16q with at least one polymorphic marker. Puring the analysis of these 21 LOH cases, 6 patients showed interstitial LOHs in which the boundary of the LOH region was defined. With two rounds of LOH analysis, five commonly occurring interstitial LOH regions were identified; 16q21-22.1, 16q22.2 - 22.3, 16q22.3, 16q23.2 and 16q23.3 - 24.1. Among the five LOH regions the 16q23.3 - 24.1 region has been reported to be related with chromosome instability. A complete physical map, which covers the 3.2 Mb region of 16q23.3 - 24.1 (D16S402 and D16S486), was constructed to identify novel candidate tumor suppressor genes. We provide the minimally tiling path map consisting of 28 BAC clones. There was one gap between NT_10422.11 and NT_019609.9 of the human genome sequence contig (NCBI sequence build 33, April 29, 2003). This gap can be filled by sequencing the R-1425M20 clone which bridges these sequence contigs.

• Advances in Traditional Medicine
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• 제5권2호
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• pp.144-149
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• 2005

The present study was designed to investigate the hypoglycaemic and hypolipidaemic activities of Qurs-e-Ziabetus 16 (QZ-16) in Streptozotocin (STZ) induced diabetic rats. QZ-16, a polypharmaceutical herbomineral formulation developed on the principles of Unani medicine is used for non-insulin dependent diabetes mellitus (NIDDM). The elevated levels of fasting blood glucose, serum levels of cholesterol, triglycerides and urea observed in rats treated with STZ (55 mg/kg body wt.) were significantly reduced by the treatment of QZ-16 (240 mg/kg, p.o.) and gliclazide (30 mg/kg, p.o.). The reduced HDL cholesterol levels were also increased by the QZ-16 and gliclazide treatments in the STZ induced diabetic rats. These data show that QZ-16 has hypoglycaemic, hypolipidaemic properties in STZ induced diabetic rats.

• 한국안전학회지
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• 제29권4호
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• pp.54-60
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• 2014

This paper describes electric shock accidents on a 4.16 kV class circuit breaker for power plant. Electric shock accidents mostly involve damage of human life, in comparison with electrical fire, rate of human death tend to be higher in electric shock accidents. Specially, in a high voltage facilities rate of human death comprised about 43.7% by electric shock accidents. If electric shock accidents happen in a 4.16 kV class circuit breaker for power plant, then the power plant discontinue power production. Electric shock accidents in a power plant have a great ripple effect such as an electric power shortage. In this paper, we analyzed electric shock accidents on a 4.16 kV class circuit breaker for power plant. From the analysis results, we confirmed a cause of electric shock accidents on a 4.16 kV class circuit breaker, it happened by defect of interlock equipment or occurrence of breakdown between first feeder contactor and shielding plate. In order to reduce electric shock accidents on a 4.16 kV class circuit breaker, the power plant should consider improvement of interlock equipment and insulation of feeder contactor in circuit breaker.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제38권2호
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• pp.101-109
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• 2012

Objectives: The inactivation of the tumor suppressor gene $p16^{INK4a}$ plays an important role in the development of malignant tumors, including oral squamous cell carcinoma. The p16 gene is involved in the p16/cyclin-dependent kinase/retinoblastoma (Rb) gene pathway of cell cycle control. The p16 protein is considered a negative regulator of this pathway. The p16 gene encodes an inhibitor of cyclin-dependent kinases 4 and 6 which regulate the phosphorylation of the retinoblastoma gene and G1 to S phase transition in the cell cycle. However, the p16 gene can lose its functionality through point mutations, loss of heterozygosity or methylation of its promoter region. Materials and Methods: In this study, the authors analyzed the correlation between various clinicopathological findings- patient age, gender and smoking, disease recurrence, tumor size, stage, and differentiation- and p16 protein expression or p16 promoter hypermethylation in 59 cases of head and neck squamous cell carcinoma. Results: The results revealed p16 protein expression and p16 promoter hypermethylation in 28 cases (47.5%) and 21 cases (35.6%), respectively, of head and neck squamous cell carcinoma. However, neither p16 protein expression nor p16 promoter hypermethylation had any statistical influence on clinicopathological findings or survival rate. Conclusion: This data, and a review of the literature, suggest that p16 promoter hypermethylation cannot yet be used as an independent prognostic factor influencing carcinogenesis, but must be considered as an important factor along with other genetic alterations affecting the pRb pathway.

• 한국방송∙미디어공학회:학술대회논문집
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• 한국방송공학회 2009년도 추계학술대회
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• pp.63-66
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• 2009

기존의 H.264/AVC 비디오 표준은 고화질 비디오 부호화를 지원하지만 고해상도에 특화된 요소 기술이 도입되지 않아 만족할만한 성능을 보이지 못한다. 현존하는 동영상 압축 표준 중 가장 뛰어난 H.264/AVC 표준의 인트라 $16{\times}16$ 예측은 매크로블록에 인접한 최대 33개의 주변 화소를 이용하여 매크로블록에 속한 256개의 화소 값을 예측한다. 특히, 전체 예측 모드 중 수직과 수평 예측 모드에서는 16개의 수직 또는 수평 위치에 위치한 주변 화소로 전체 매크로블록 내의 화소 값을 예측하므로 매크로 블록의 끝으로 갈수록 예측의 정확도가 떨어져 부호화 비트가 증가한다. 고화질 영상에서는 인트라 $16{\times}16$ 모드로 부호화되는 블록이 많으므로 수행되므로 인트라 $16{\times}16$ 예측의 정확도를 높일 수 있는 기술이 필요하다. 본 논문에서는 기존의 H.264/AVC의 예측 방법보다 예측 정확도가 높은 새로운 라인 기반 $16{\times}16$ 인트라 예측 방법을 제안한다. 일반적으로 편평한 특성을 보이는 인트라 $16{\times}16$ 블록이라도 좀 더 가까운 화소를 참조 화소로 사용하면 예측의 정확도를 높여 부호화 비트를 줄일 수 있다. 이를 이용하여 제안하는 알고리즘에서는 인트라 $16{\times}16$ 블록에서 16개 화소 한 줄을 단위로 예측 및 부호화를 수행한다. 1080p HD급 테스트 영상을 이용하여 실험한 결과, 기존의 H.264/AVC FRExt High 프로파일에 비해 평균 약 6.92%의 부호화 비트를 감소시킬 수 있음을 보였다.

• 한국통신학회논문지
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• 제37C권11호
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• pp.1045-1053
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• 2012

Link-16은 가장 널리 사용 중인 전술데이터링크로써 TDMA(Time Division Multiple Access)를 기반으로 동작한다. Link-16은 안정적이지만 전송 속도가 매우 낮아 전술 메시지, 음성 등 작은 사이즈의 데이터 전송을 지원한다. 그러나 최근 효과중심작전(EBO: Effect-Based Operation)에 대한 관심이 증가하면서, Link-16을 통해 이미지와 같은 상황 인식 정보를 전송하려는 동향이 있다. 이미지는 기존 메시지에 비해 크기가 매우 크기 때문에 Link-16의 Static TDMA가 아닌 별도의 TDMA 스케줄링이 필요하다. 따라서 본 논문에서는 Link-16의 MAC을 진화시킨 Link-16K를 제안하였다. Link-16K는 Link-16과 호환성을 유지한다. 그리고 이미지 전송을 효과적으로 지원하기 위해 DTDMA(Dynamic TDMA), 새로운 재전송 방법, 새로운 패킹 방법을 포함한다. 제안하는 아이디어의 시뮬레이션 결과를 통해 이미지 전송 시간이 단축되었고, 채널 효율성이 높아졌음을 확인하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권16호
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• pp.7161-7165
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• 2015

Background: Esophageal squamous cell carcinoma (ESCC) is a common cancer in the north east of India. The present study concerned the prevalence of human papilloma virus (HPV) in the ESCC in north eastern India and its impact on response to chemotherapy. Materials and Methods: p16 expression, a surrogate marker for HPV infection was assessed in 101 pre-treatment biopsies of locally advanced ESCC, reported from a comprehensive cancer centre in north east India, using immunohistochemistry. All patients received neo-adjuvant chemotherapy. Response was assessed clinically and histopathologically with attention to p16 expression. Results: p16 was expressed in 22% of ESCC (22 out of 101) and was more prevalent in patients who were more than 45 years of age (P=0.048). p16 positive tumors appeared more commonly in the upper 2/3 of the thoracic esophagus (18 in 22). Nine of the 22 (41%) p16 positive tumors achieved pathologic complete response following neo-adjuvant chemotherapy (P=0.008). There was a trend towards reduced mortality in this group (P=0.048). Some 9 of the 20 (45%) patients who achieved pathologic complete response were p16 positive. Conclusions: Expression of p16 in ESCC correlates with higher rate of pathologic complete remission in patients undergoing neo adjuvant chemotherapy and could be a predictive marker for response assessment.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2006년도 추계학술대회 논문집 Vol.19
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• pp.141-142
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• 2006

Thermoelectric properties and microstructures of $Sr_{8-x}Ba_xGA_{16}Ge_{30}$ alloys fabricated by the arc-melting method were investigated. The alloys with the nominal composition of $Sr_8Ga_{16}Ge_{30}$ and $Ba_8Ga_{16}Ge_{30}$ were the single-phase alloys, while those of $Sr_4Ba_4Ga_{16}Ge_{30}$ and $Sr_2Ba_6Ga_{16}Ge_{30}$ were two-phases alloys. Electrical resistivity and the Seebeck coefficient for both two-phases alloys were higher in magnitude than those of the single-phase alloys between room temperature and 873K The thermal conductivities for both two-phase alloys were reduced with respect to those of the single-phase alloys in the whole temperature range. The maximum values of ZT for $Sr_4Ba_4Ga_{16}Ge_{30}$ and $Sr_2Ba_6Ga_{16}Ge_{30}$ were achieved with the values of 0.69 at 753K and 0.51 at 754K, respectively, while those of $Sr_8Ga_{16}Ge_{30}$ and $Ba_8Ga_{16}Ge_{30}$ were 0.86 at 758K and 0.76 at 943K, respectively.

• 한국식품과학회지
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• 제30권5호
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• pp.1018-1023
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• 1998

방사선 조사한 라면 건조 스프에서 hydrocarbon류를 검출하여 이를 라면 스프의 방사선 조사 여부를 검지하는 데 활용하고자 하였다. 스프 재료를 개별, 또는 배합 전후에 방사선 조사하여, hexane으로 지방을 추출하고 Florisil column으로 분리한 hydrocarbon류를 GC를 사용하여 분석하였다. 10 kGy 조사한 팜유, 고춧가루, 참깨에서 비조사 시료에서는 검출되지 않는 hydrocarbon C17:2, C16:3, C17:1, C16:2 등이 검출되었다. 비조사 재료만을 사용하여 배합한 스프에서는 C17:2, C16:3, C17:1, C16:2가 검출되지 않았다. 팜유 또는 참깨만을 방사선 조사한 것을 사용한 배합 스프에서는 C17:2, C16:3, C17:1, C16:2이 검출되었으며, 고춧가루만 방사선 조사한 것을 사용한 배합 스프에서는 C17:2와 C16:3이 검출되었다. 비조사 재료를 배합한 후 방사선 조사하였을 때, 1 kGy 조사 시료에서 C17:2, C17:1, C16:2가 검출되었고, 5 kGy 및 10 kGy 조사한 시료에서 C17:2, C16:3, C17:1, C16:2가 다량 검출되었다.