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Root Colonization and ISR-mediated Anthracnose Disease Control in Cucumber by Strain Enterobacter asburiae B1

  • Bharathkumar, S.;Park, Jin-Woo;Han, Ji-Hee;Park, Kyung-Seok
    • The Plant Pathology Journal
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    • v.25 no.4
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    • pp.333-343
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    • 2009
  • Here, we show that an endophytic bacterial strain, Enterobacter asburiae B1 exhibits the ability to elicit ISR in cucumber, tobacco and Arabidopsis thaliana. This indicates that strain B1 has a widespread ability to elicit ISR on various host plants. In this study, E. asburiae strain B1 did not show antifungal activity against tested major fungal pathogens, Colletotrichum orbiculare, Botrytis cinerea, Phytophthora capsici, Rhizoctonia solani, and Fusarium oxysporum. Moreover, the siderophore production by E. asburiae strain B1 was observed under in vitro condition. In greenhouse experiments, the root treatment of strain B1 significantly reduced disease severity of cucumber anthracnose caused by fungal pathogen C. orbiculare compared to nontreated control plants. By root treatment of strain B1 more than 50% disease control against anthracnose on cucumber was observed in all greenhouse experiments. Simultaneously, under the greenhouse condition, the soil drench of strain B1 and a chemical inducer benzothiadiazole (BTH) to tobacco plants induced GUS activity which is linked with activation of PR promoter gene. Furthermore, in Arabidopsis thaliana plants the soil drench of strain B1 induced the defense gene expression of PR1 and PDF1.2 related to salicylic acid and jasmonic acid/ethylene signaling pathways, respectively. In this study, for the main focus on root colonization by strain B1 associated with defense responses, bacterial cells of strain B1 was tagged with the gfp gene encoding the green fluorescent protein in order to determine the colonization pattern of strain B1 in cucumber. The gfp-tagged B1 cells were found on root surface and internal colonization in root, stem, and leaf. In addition to this, the scanning electron microscopy observation showed that E. asburiae strain B1 was able to colonized cucumber root surface.

Changes of Emergent Period and Body Volume of Chironomus riparius Exposure to Di(2-ethyl-hexyl)-phthalate

  • Kwak Inn-Sil;Soh Ho Young;Lee Wonchoel
    • Korean Journal of Environmental Biology
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    • v.23 no.4
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    • pp.357-361
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    • 2005
  • The exposed strain of C. riparius treated with di (ethyl- hexyl)-phthalate (DEHP) did not result in a consistent relationship between mortality or sex ratio and chemical concentrations. And after treating with DEHP, the emergent female from the exposed strain appeared to be fatty with a large body volume comparing with the non - exposed strain. The emergent period (EP) was especially different between the exposed fourth strain and the non-exposed strain; generally the exposed strain was $7\~10$ days, and non - exposed strain was $17\~24$ days. Regarding sustainable exposed effects, the EP, the first emergent day (FED), and the body volume (BV) could be suggested as suitable bio - markers for detecting of exposure to various EDCs.

Temperature Compensation of a Fiber Optic Strain Sensor Based on Brillouin Scattering

  • Cho, Seok-Beom;Lee, Jung-Ju;Kwon, Il-Bum
    • Journal of the Optical Society of Korea
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    • v.8 no.4
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    • pp.168-173
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    • 2004
  • Brillouin scattering-based fiber optic sensors are useful to measure strain or temperature in a distributed manner. Since the Brillouin frequency of an optical fiber depends on both the strain and temperature, it is very important to know whether the Brillouin frequency shift is caused by the strain change or temperature change. This article presents a temperature compensation technique of a Brillouin scattering-based fiber optic strain sensor. Both the changes of the Brillouin frequency and the Brillouin gain power is observed for the temperature compensation using a BOTDA sensor system. Experimental results showed that the temperature compensated strain values were highly consistent with actual strain values.

Rate-sensitive analysis of framed structures part II: implementation and application to steel and R/C frames

  • Fang, Q.;Izzuddin, B.A.
    • Structural Engineering and Mechanics
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    • v.5 no.3
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    • pp.239-256
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    • 1997
  • The companion paper presents a new three-parameter model for the uniaxial rate-sensitive material response, which is based on a bilinear static stress-strain relationship with kinematic strain-hardening. This paper extends the proposed model to trilinear static stress-strain relationships for steel and concrete, and discusses the implementation of the new models within an incremental-iterative solution procedure. For steel, the three-parameter rate-function is employed with a trilinear static stress-strain relationship, which allows the utilisation of different levels of rate-sensitivity for the plastic plateau and strain-hardening ranges. For concrete, on the other hand, two trilinear stress-strain relationships are used for tension and compression, where rate-sensitivity is accounted for in the strain-softening range. Both models have been implemented within the nonlinear analysis program ADAPTIC, which is used herein to provide verification for the models, and to demonstrate their applicability to the rate-sensitive analysis of steel and reinforced concrete structures.

Mechanical and electro-mechanical analysis in differently stabilized GdBCO coated conductor tapes with stainless steel substrate

  • Nisay, Arman R.;Shin, Hyung-Seop
    • Progress in Superconductivity and Cryogenics
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    • v.15 no.2
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    • pp.29-33
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    • 2013
  • The understanding of the strain dependence of critical current, $I_c$, in the reversible region is important for the evaluation of the performance of coated conductor (CC) tapes in practical applications. In this study, the stress/strain tolerance of $I_c$ in GdBCO CC tapes with stainless steel substrate stabilized by additional Cu and brass laminate was analyzed quantitatively through $I_c$-strain measurement at 77 K under self-field. The variation in irreversible strain limits of CC tapes by the addition of stabilizing layers was analyzed through the consideration of the pre-strain induced on the GdBCO coating film. The results were then compared with the ones previously reported for GdBCO CC tapes with Hastelloy substrate. As a result, GdBCO CC tapes with stainless steel substrate showed much higher strain tolerance of $I_c$ as compared with those adopting Hastelloy substrate.

Temperature effect analysis of a long-span cable-stayed bridge based on extreme strain estimation

  • Yang, Xia;Zhang, Jing;Ren, Wei-Xin
    • Smart Structures and Systems
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    • v.20 no.1
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    • pp.11-22
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    • 2017
  • The long-term effect of ambient temperature on bridge strain is an important and challenging problem. To investigate this issue, one year data of strain and ambient temperature of a long-span cable-stayed bridge is studied in this paper. The measured strain-time history is decomposed into two parts to obtain the strains due to vehicle load and temperature alone. A linear regression model between the temperature and the strain due to temperature is established. It is shown that for every $1^{\circ}C$ increase in temperature, the stress is increased by 0.148 MPa. Furthmore, the extreme value distributions of the strains due to vehicle load, temperature and the combination effect of them during the remaining service period are estimated by the average conditional exceedance rate approach. This approach avoids the problem of declustering of data to ensure independence. The estimated results demonstrate that the 95% quantile of the extreme strain distribution due to temperature is up to $1.488{\times}10^{-4}$ which is 2.38 times larger than that due to vehicle load. The study also indicates that the estimated extreme strain can reflect the long-term effect of temperature on bridge strain state, which has reference significance for the reliability estimation and safety assessment.

Strain Improvement of Candida tropicalis for the Production of Xylitol: Biochemical and Physiological Characterization of Wild-type and Mutant Strain CT-OMV5

  • Rao Ravella Sreenivas;Jyothi Cherukuri Pavanna;Prakasham Reddy Shetty;Rao Chaganti Subba;Sarma Ponnupalli Nageshwara;Rao Linga Venkateswar
    • Journal of Microbiology
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    • v.44 no.1
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    • pp.113-120
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    • 2006
  • Candida tropicalis was treated with ultraviolet (UV) rays, and the mutants obtained were screened for xylitol production. One of the mutants, the UV1 produced 0.81g of xylitol per gram of xylose. This was further mutated with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), and the mutants obtained were screened for xylitol production. One of the mutants (CT-OMV5) produced 0.85g/g of xylitol from xylose. Xylitol production improved to 0.87 g/g of xylose with this strain when the production medium was supplemented with urea. The CT-OMV5 mutant strain differs by 12 tests when compared to the wild-type Candida tropicalis strain. The XR activity was higher in mutant CT-OMV5. The distinct difference between the mutant and wild-type strain is the presence of numerous chlamvdospores in the mutant. In this investigation, we have demonstrated that mutagenesis was successful in generating a superior xylitol-producing strain, CT-OMV5, and uncovered distinctive biochemical and physiological characteristics of the wild-type and mutant strain, CT-OMV5.

Multiscale features and information extraction of online strain for long-span bridges

  • Wu, Baijian;Li, Zhaoxia;Chan, Tommy H.T.;Wang, Ying
    • Smart Structures and Systems
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    • v.14 no.4
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    • pp.679-697
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    • 2014
  • The strain data acquired from structural health monitoring (SHM) systems play an important role in the state monitoring and damage identification of bridges. Due to the environmental complexity of civil structures, a better understanding of the actual strain data will help filling the gap between theoretical/laboratorial results and practical application. In the study, the multi-scale features of strain response are first revealed after abundant investigations on the actual data from two typical long-span bridges. Results show that, strain types at the three typical temporal scales of $10^5$, $10^2$ and $10^0$ sec are caused by temperature change, trains and heavy trucks, and have their respective cut-off frequency in the order of $10^{-2}$, $10^{-1}$ and $10^0$ Hz. Multi-resolution analysis and wavelet shrinkage are applied for separating and extracting these strain types. During the above process, two methods for determining thresholds are introduced. The excellent ability of wavelet transform on simultaneously time-frequency analysis leads to an effective information extraction. After extraction, the strain data will be compressed at an attractive ratio. This research may contribute to a further understanding of actual strain data of long-span bridges; also, the proposed extracting methodology is applicable on actual SHM systems.

Fabrication of Inkjet Printed Strain Gauge Using PEDOT:PSS (PEDOT:PSS기반 잉크젯 프린팅 스트레인 게이지의 제작)

  • Kye, Ji Won;Han, Dong Cheul;Shin, Han Jae;Yeom, Se-hyuk;Lee, Wanghoon
    • Journal of Sensor Science and Technology
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    • v.26 no.1
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    • pp.56-59
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    • 2017
  • This paper presents the Inkjet-printed strain gauge using PEDOT:PSS. The strain gauge (width 0.6 mm, length 20 mm, thickness $0.3{\mu}m$) was printed on the PET film using PEDOT:PSS ink. The resistance variation of the fabricated strain gauge was measured by the digital multi-meter with the displacement range of -4 to 10 mm. As the measured result, resistance variation (${\Delta}R/R_0$) has approximately 0.75%, linearity of 99.87%. The fabricated strain gauge is expected to the various applications such as tape type pressure sensor, PMS(pressure mapping sensor), wearable devices.

Development of the Pin Type Load-cell Using Strain Gauge (Strain Gauge를 이용한 핀형 로드셀 개발)

  • Lee, Dong-Wook;Park, Min-Hyuk;Lee, Gye-Gaong;Kim, In-Hwan;Lee, Seok-Soon
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.13 no.4
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    • pp.75-82
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    • 2014
  • A pin-type load-cell which uses shear-type strain gauges was developed to measure the tension of a wire in a winch. A finite element analysis was performed to determine the locations of the strain gauges. All of the shear-type strain gauges were attached onto parts that undergo regularly shear stress distributions. A Wheatstone bridge circuit was used to connect each of the gauges and to measure the strains. Linearity within the 5% error range was noted when testing the pin-type load-cell.