• Journal of Animal Science and Technology
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• v.51 no.2
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• pp.171-176
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• 2009

A bacterial strain producing intracellular phytase was isolated from cultivable soil near cowsheds and identified as a fluorescent Pseudomonas sp. BUN1. The BUN1 phytase, partially purified by cation and anion exchange chromatography, exhibited its optimal activity at $40^{\circ}C$ and pH 5.5. As for substrate specificity, it was very specific for phytate and showed little activity on other phosphorylated conjugates. Its activity was greatly inhibited by metal ions such as $Cu^{2+}$, $Cd^{2+}$, and $Zn^{2+}$. Addition of corn starch to PSM (phytasesynthetic medium) [0.5% sodium phytate, 0.5% $(NH_4)_2SO_4$, 0.5% KCl, 0.01% $MgSO_4\cdot7H_2O$, 0.01% $CaCl_2\cdot2H_2O$, 0.01% NaCl, 0.001% $FeSO_4\cdot7H_2O$, 0.001% $MnSO_4\cdot4H_2O$; pH 6.5] for the phytase production significantly induced its enzyme activity in comparison with other carbon sources tested.

• Applied Biological Chemistry
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• v.14 no.1
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• pp.99-102
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• 1971

These experiment were conducted to study the effects of various nitrogen sources and inorganic salts upon the growth of Saccharomyces rouxii T-9, and the results obtained were as follows: 1) The strain showed the most rapid growth in the case of 2.5 to 5.0% addition of peptone as an organic nitrogen source, and 1.0% addition of $(NH_4)_2HPO_4$ as an inorganic nitrogen source respectively to the media containing no NaCl. 2) Its growth was the most rapid in the case of 1.5% addition of yeast extracts as an organic nitrogen sources, and 5% addition of urea as an inorganic nitrogen source respectively to the media containing 20% of NaCl. 3) Its growth was the most rapid in the case of 1% addition of $NaNO_3$ as an inorganic salt to the media containing no NaCl, while to the media containing 20% or 26% of NaCl its growth was more rapid in the case of 5% addition of $NaNO_3$ than 1% addition. 4) Its growth was more rapid in the case of 0.5% addition of $MgSO_4\;and\;CaCl_2$ respectively to the media containing 20% of NaCl than to the media containing no NaCl, and was rapid in the case of 1% addition of $MgCl_2$ to the media containing 26% of NaCl.

• Journal of the Korea institute for structural maintenance and inspection
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• v.20 no.5
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• pp.109-115
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• 2016

A demand of high bearing capacity of piles to resist heavy static loads has been increased. For this reason, the utilization of large diameter PHC piles including a range from 700 mm to 1,200 mm have been increased and applied to the construction sites in Korea recently. In this study, in order to increase the flexural strength capacity of the PHC pile, the large diameter composite PHC pile reinforced by in-filled concrete and reinforcement was developed and manufactured. All the specimens were tested under four-point bending setup and displacement control. From the strain behavior of transverse bar, it was found that the presence of transverse bar was effective against crack propagation and controlling crack width as well as prevented the web shear cracks. The flexural strength and mid-span deflection of LICPT specimens were increased by a maximum of 1.08 times and 1.19 times compared to the LICP specimens. This results indicated that the installed transverse bar is in an advantageous ductility performance of the PHC piles. A conventional layered sectional analysis for the pile specimens was performed to investigate the flexural strength according to the each used material. The calculated bending moment of conventional PHC pile and composite PHC pile, which was determined by P-M interaction curve, showed a safety factor 1.13 and 1.16 compared to the test results.

• Journal of the Korea institute for structural maintenance and inspection
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• v.21 no.6
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• pp.10-15
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• 2017

The refraction expansion joints have been newly developed by complementing the problems of shock, noise, replaceability, displacement in the direction perpendicular to bridge, vertical difference, which are problems of existing expansion joints. The Refraction expansion joints are characterized by continuous surfaces with small impact and low noise. The behavior of the Refraction expansion joints performs the bridge expansion behavior by rotation of the link. In the rotational behavior of the link, the bolt is the central axis of the behavior. Therefore, it can be said that the durability of the bolt is very important. However, the theoretical and experimental verification of the bolt durability of the Refraction expansion joint is lacking. In this paper, to verify the fatigue durability of the bolt, test specimens with a 300 mm Refraction expansion joint were fabricated. A strain gauge dedicated to the bolt was installed inside the manufactured test specimen bolt. The test method was applied in accordance with KS F 4425. The fatigue durability of the bolts assembled inside the diaphragm expansion joint was confirmed by the repeated fatigue test of 2,000,000 cycles.

• Journal of the Korea institute for structural maintenance and inspection
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• v.21 no.6
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• pp.196-205
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• 2017

In precast deck system, structural performance and serviceability are mostly determined by the connection methods between the precast decks. This study proposes precast deck system with asymmetric ribbed connection details using High Strength Fiber Reinforced Concrete(HSFRC) with filler. To verify the proposed method, the flexural performance experiment was carried out with variation of joint cross section type and splice rebar details. From the test results, regardless of joint details, spliced tensile rebars of specimens were deformed to yielding strain level. Also, all types of specimens resulted in sufficient flexural performance. These test results show that the minimum lap splice length specified in current Korea Highway Bridge Design Code is conservative for precast deck joint using HSFRC. Therefore, splice details can be simplified and joint width can be reduced by using HSFRC with filler between the precast decks, and the proposed precast deck systems can be applied to the connection part of precast decks effectively.

• Journal of the Korea institute for structural maintenance and inspection
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• v.22 no.4
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• pp.1-9
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• 2018

The development of new technology and process in industrial Plant which builds integrated structures, facilities and systems. Has become a key element for strengthening its competitiveness. Although domestic industrial Plant has demonstrated excellence in technology with a persistent increase in order quantity and orders received, the technology gap between countries has narrowed due to global construction trend. Therefore, it is necessary to develop new technology that could help overcome constraints and limitations of the current one to follow the trend in the age of unlimited competition. This study has focused on assembly technology of Pipe-rack joint connection in an effort to strengthen technological competitiveness in industrial Plant. Through an analysis of earlier studies on Pipe-rack and a coMParative analysis of strengths and weaknesses of current assembly technology of it, a new design plan has been made to improve it efficiently. In doing this, standards for design factors of both structural and performance features have been drawn, and value of stress, strain, moment and rotation has been calculated using finite element analysis. As a result, installation technology of modular type Pipe-rack, which has not been developed in Korea and is differentiated from the current one, has been developed. It is considered that the technology reduces work time and saves cost due to simplified joint connection of steel structure, unlike the current one. Moreover, since it is installed without a welding process in the field, industrial accidents would be reduced, which is likely to have economic competitiveness and satisfy.

• Journal of the Korea institute for structural maintenance and inspection
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• v.22 no.4
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• pp.100-107
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• 2018

The objective of this study is to assess the strengthening effects of fiber reinforced polymer(FRP) sheets such as Carbon fiber, Glass fiber, and PET(polyethylene terephthalate) on reinforced concrete flexural members. Variables of theoretical analysis are types of strengthening materials, material properties and amount of strengthening materials. A virtual flexural member without FRP sheets was created as a control specimen to understand the structural behavior of the non-strengthened specimen in terms of elastic and ultimate cross section. In total, 11 specimens including one non-strengthened and ten strengthened specimens were investigated. Various variables such as types of strengthening, strengthening properties, and amount of strengthening were studied to compare the behavior of the control specimen with those of strengthened specimens with regard to moment-curvature relationship. Results of theoretical analysis showed that the moment capacity of strengthened specimens was superior to that of the control specimen. However, the control specimen indicated the best ductility among all the specimens. As the amount of strengthening increased, flexural performance was improved. Furthermore, the results indicated that the ductile effect of members was affected by the ultimate strain of FRP sheets. The strengthening effect on the damaged member was similar to that on the non-damaged one since there was less than 10% difference in terms of flexural strength and ductility. Therefore, even if a damaged member is treated as non-damaged for analysis there is probably no noticeable difference.

• Geotechnical Engineering
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• v.13 no.4
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• pp.37-54
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• 1997

This paper is to investigate the bearing capacity and the failure mechanism of slope subjected to strip surcharges adjassent to embankment slope of sandy soil. Parametric model tests under plain strain condition were performed by changing width of footing, relative density of slope materials, and position of footing from the crest of slopes. For model tests, Jumunjin standard sand was used as the slope material and its relative density was 45% and 70%, respectively. The angle of slope was formed with 1 : 1.5 and 1 2. Rigid model footings, made of aluminuu were used with their widths of 4, 7, 10 and 12cm. For the position of model footing, position ratios, distance of model footing from the crest of slope divided by footing width, were 0, 0.5, 1, 2, 3, 4, 5. Failure mechanism was observed by using ink colored sands and markers inserted in model slopes. Ultimate bearing capacity obtained from tests was analyzed and compared with limit equilibrium method, limit analysis method and empirical equation. Characteristics of load-settlement curves and failure mechanism were also analyzed and compared with the existing theories. Thus, their effects on ultimate bearing capacity of model footing adjacent to slope were assessed.

• Journal of the Korean Geosynthetics Society
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• v.16 no.4
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• pp.83-92
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• 2017

This paper deals with numerical analysis of behavior of curved mechanically stabilized earth(MSE) walls with geosynthetics reinforcement. Unlike typical concrete retaining walls, MSE wall enables securing stability of higher walls without being constrained by backfill height and is currently and widely used to create spaces for industrial and residential complexes. The design of MSE walls is carried out by checking external stability, similarly to the external checks of conventional retaining wall. In addition, internal stability check is mandatory. Typical stability check based on numerical analysis is done assuming 2-dimensional condition (plane strain condition). However, according to the former studies of 3-dimensional MSE wall, the most weakest part of a curved geosynthetic MSE wall is reported as the convex location, which is also identified from the studies of the laboratory model tests and field monitoring. In order to understand the behaviour of the convex location of the MSE wall, 2-dimensional analysis clearly reveals its limitation. Furthermore, laboratory model tests and field monitoring also have restriction in recognizing their behaviour and failure mechanism. In this study, 3-dimensional numerical analysis was performed to figure out the behaviour of the curved part of the geosynthetic reinforced wall, and the results of the straight-line and curved part in the numerical analysis were compared and analysed. In addition, the behaviour characteristics at each condition were compared by considering the overburden load and relative density of backfill.

• The Korean Journal of Zoology
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• v.38 no.3
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• pp.340-347
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• 1995

We have used two kinds of P element constructs, Pc[(ry+)B] and p[(ry+)$\Delta$SX9], for genetic transformation by microinjection of D. melanogaster. Pc[(ry+)B] construct carrying the rosy gene within an autonomous P element was injected into a true M strain caring the ry506. mutation. The source of transposase for microinjection and transformation was provided by a P element helper plasmid designated p-$\Delta$2-3hs$\pi$, which was co-injected with nonautonomous P[(ry+)$\Delta$SX9] construct into same ry506 M strains. A dechorination method was adopted and 35 independent transformed lines were obtained froin 1143 G0 Injected (35/1143). About 20% of the injected embryos eclosed as adults. Among G0 eclosed flies, approximately 40% exhibited eye color that was similar to wild-type (ry+), but about 60% of fertile G0 transformed lines appeared to have no G1 transformants. Therefore it is unlikely that G0 expression requires integration of the rosy transposon into chromosomes. Pc[(ry+)B] and P[(ry+)$\Delta$SX9] constructs were found to be nearly same in the frequency of element-mediated transformation. On the basis of these results, nonautonomous P elements constructs could he used as same effective vectors in P element-mediated transformation for introducing and fixing genes in insect populations.