• Title/Summary/Keyword: K strain

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Ettect of Diets Containing Dried Whey and Chitin on Growth Rate of Broiler Chicks (Dried Whey와 Chitin의 첨가(添加)가 Broiler Chick의 성장(成長)에 미치는 영향(影響)에 관한 연구(硏究))

  • Lee, Mee-Sook;Mo, Su-Mi
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.14 no.2
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    • pp.192-201
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    • 1985
  • Two experiments, utilizing 204 broiler chicks of the Maniker strain, were conducted to study the effects of dried whey and chitin on the growth of chicks. In the first experiment, diets containing 5%, 10%, or 15% dried whey, or a control diet with no whey, were fed to chicks from 1 day to 4 weeks of age. There were no significant differences among the dietary groups, with respect to the rate of growth or protein or feed efficiency. In the second experiment, chicks were fed with diets containing no whey, 2% chitin, 20% dried whey, or 20% dried whey plus 2% chitin, from 1 day to 4 weeks of age. Adverse effects (diarrhea, crooked toes and enlarged cecum) were observed in the group fed 20% dried whey. The protein efficiency ratio (PER) and feed efficiency ratio (FER) tended to improve in the dietary groups with dried whey, as compared to the control group. No significant differences were observed in the total carcass nitrogen and lipid levels of dietary groups. But the chitin-supplemented diets tended to improve on the growth rate compared to the groups without chitin. The results of the two experiments suggest that the growing broiler chick can tolerate up to 15% dried whey in the diet, without any harmful effect on growth. Also, chitin may improve the poorer growth rate of chicks fed dried whey, but not all of the adverse effects of whey.

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Effects of Surface Offcut Angle of GaAs Substrate on Dislocation Density of InGaP Epilayers (GaAs기판의 표면 Offcut각도가 InGaP 에피막의 전위밀도에 미치는 영향)

  • 이종원;박경수;이종식
    • Journal of the Microelectronics and Packaging Society
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    • v.9 no.3
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    • pp.49-56
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    • 2002
  • In this study, the InGaP epilayers were grown on the exact and the $2^{\circ}$, $6^{\circ}$, $10^{\circ}$ of cut GaAs substrates by metal-organic vapor phase epitaxy, and the effects of interfacial elastic strains determined by the substrate offcut angle upon the resulting dislocation density of epilayer were investigated for the first time. The elastic strains were obtained from lattice mismatch and lattice misfit by TXRD, and the dislocation densities from epilayer x-ray FWHM. For the offcut angle range used in this study, the elastic strain was maximum and x-ray FWHM minimum at offcut angle $6^{\circ}$. From 11K PL measurements, PL wavelength was found to decrease with an increase of offcut angle. PL intensity was maximum at offcut angle $6^{\circ}$. TEM results showed that the electron diffraction pattern was of typical zincblende structure, and that the dislocation density was minimum for substrate offcut angle $6^{\circ}$. The results obtained in this study, along with the device fabrication process and beam characteristics, clearly demonstrated that the optimum substrate offcut angle for the InGaP/GaAs heterostructures is $6^{\circ}$.

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Interspecific Variation in the Protoplast Formation of the Genus Cellulomonas (Cellulomonas속 종간의 원형질체 형성조건의 차이에 대하여)

  • Lee, Eun-Ju;Bae, Moo
    • Korean Journal of Microbiology
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    • v.24 no.2
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    • pp.154-160
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    • 1986
  • In order to develope interspecific fusion of the genus Cellulomonas capable of assimilation cellulose, the optimun conditions for the protoplast formation was investigated to examine the susceptibility of cell wall, between different species of the same genus using scanning electron microscope. The variation in the susceptibilities of Cellulomonas sp. CS 1-1 and C. flavigena to lysozyme treatment were considerably remarkable, although they belong to the same genus. The rate of protoplast formation of CS1-1 was 99.9% being treated with lysozyme $(100{\mu}g/ml)$ for 30 minute and that of C. flavigena was about 80% being treated at the concentration of $600{\mu}g/ml$ of lysozyme for 6 hours. The susceptibility of cell wall to the lysozyme treatment on protoplast formation of the strain, CS1-1 seems not to be depend on the cultural periods of cells. On the contrary, that of C. flavigena was considerably depend on the periods. Cells of C. flavigena at mid exponential phase could be more efficiently converted to protoplast cells than those at late exponential phase be done. The rate of the protoplast formation was 95%, when cells of C. flavigena at mid exponential phase were treated with lysozyme $600{\mu}g/ml$ for 6 hours and observed by SEM. In the evalution of protoplast formation of the CS1-1 results of counting method in plate after osmotic shock treatment were similar to the results of the direct observation method by means of SEM. But in the case of C. flavigena the latter method was much more reliable than the former, because the differences between the number of spheroplasts and protoplasts were not able to figure out on conuting the number of protoplast after osmotic shock tretment.

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MOLECULAR CLONING AND SEQUENCE ANALYSIS OF THE GENE FOR THE HEMIN-BINDING PROTEIN FROM Prevotella intermedia (Prevotella intermedia에서의 Hemin 결합 단백질 유전자의 분리 및 염기서열 분석)

  • Kim, Shin;Kim, Sung-Jo
    • Journal of the korean academy of Pediatric Dentistry
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    • v.33 no.2
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    • pp.304-310
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    • 2006
  • Prevotella intermedia is one of the most frequently implicated pathogens in human periodontal disease and has a requirement for hemin for growth. This study has identified a hemin-binding P. intermedia protein by expression of a P. intermedia genomic library in Escherichia coli, a bacterium which does not require or transport exogenous hemin. The genomic library of P. intermedia was constructed into plasmid pUC18, transformed into Escherichia coli strain $DH5{\alpha}$, and screened for recombinant clones using heminbinding activity by plating onto hemin-containing agar. Approximately 5,000 recombinant E. coli colonies were screened onto LB-amp-hemin agar, single clone(pHem1) was exhibited a clearly pigmented phonotype. The 2.5kb insert DNA of pHem1 was determined by restriction enzyme mapping. Southern blot analysis of BamHI, BglII, EcoRI, HindIII and PstI-digested P. intermedia DNA indicated that single copy of the gene was present in the genome. Northern blot analysis revealed that the size of transcript was approximately 1.8 kb. The cloned gene contained a single ORF, consisting of approximately 850-residue amino acids. A BLAST search of the Institute for Genomic Research genes with similar nucleotide sequence revealed no significant similarity It needs further investigation to clarify the mechanisms of heme uptake in P. intermedia.

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Shear Strength of Ultra-High Performance Fiber-Reinforced Concrete(UHPFRC) I-shaped Beams without Stirrup (강섬유 보강 초고성능 콘크리트(UHPFRC) I형 보의 전단 강도)

  • Lee, Ji-Hyung;Hong, Sung-Gul
    • Journal of the Korea Concrete Institute
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    • v.29 no.1
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    • pp.53-64
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    • 2017
  • Ultra-high performance fiber-reinforced concrete (UHPFRC) is characterized by a post-cracking residual tensile strength with a large tensile strain as well as a high compressive strength. To determine a material tensile strength of UHPFRC, three-point loading test on notched prism and direct tensile test on doubly notched plate were compared and then the design tensile strength is decided. Shear tests on nine I-shaped beams with varied types of fiber volume ratio, shear span ratio and size effect were conducted to investigate shear behavior in web. From the test results, the stress redistribution ability represented as diagonal cracked zone was quantified by inclination of principal stress in web. The test results shows that the specimens were capable of resistance to shear loading without stirrup in a range of large deformation and the strength increase with post-cracking behavior is stable. However at the ultimate state all test specimens failed as a crack localization in the damaged zone and the shear strength of specimens is affected by shear span ratio and effective depth. Strength predictions show that the existing recommendations should be modified considering shear span ratio and effective depth as design parameters.

Studies of Insecticide Resistance in Green Peach Aphids, Myzus persicae(Sulz) III. Acephate Resistance, Cross-Resistance, and Esterase Isozymes (복숭아혹진딧물의 살충제 저항성(抵抗性)에 관한 연구(硏究) III. Acephate저항성(抵抗性) 발달(發達), 교차저항성(交叉抵抗性) 및 Esterase Isozymes)

  • Choi, Seung-Yoon;Kim, Gil-Hah
    • Korean journal of applied entomology
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    • v.25 no.2 s.67
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    • pp.99-105
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    • 1986
  • The green peach aphids(Myzus persicae) collected in a field had been successively selected by acephate(O, S-dimethyl N-acetyl phosphoroamidothioate) in the laboratory. The selected aphid strain in the 20th generation demonstrated relatively high resistance to acephate as well as relatively high cross-resistance to cypermethrin and oxydemeton-methyl, except pirimicarb. The different esterase isozymes with the strains were detected by the agarose gel electrophoresis and among the isozymes the band of ${\beta}-2$ was only specific for the acephate resistant strains.

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Optimum Reinforcement Conditions of Large Diameter Reinforcement for Steep Slope of Conventional Railway Embankment under Train Loading (기존선 성토사면 급구배화를 위한 열차 하중 하 대구경 봉상보강재의 최적 보강조건)

  • Kwak, Chang-Won;Kim, Dae-Sang
    • Journal of the Korean Geotechnical Society
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    • v.32 no.11
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    • pp.43-50
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    • 2016
  • A reinforcement is required to ensure the structural safety in case of railway embankment excavation under railway load. A large diameter soil nailing with concrete wall is applied as the reinforcement method instead of the conventional soil nailing system. In this study, a series of 3 dimensional numerical analyses are performed to investigate the optimum reinforcement considering 15 different conditions based on the length, lateral spacing, diameter, and inclination of the reinforcement. The interface between soil nail and perimetric grout is considered by means of cohesion, stiffness and perimeter of the grout. 0.3 m of reinforcement diameter is assessed as the most appropriate based on the economical viewpoint though ground displacement decreases with the increase of diameter, however the difference of displacement is negligible between 0.4 m and 0.3 m of diameter. Surface settlement, lateral displacement of wall, and stress of reinforcement are calculated and economic viewpoint to reinforce embankment considered. Consequently, the optimum reinforcement conditions considering those factors are evaluated as 3 m in length, 0.3 m in diameter, 1.5 m in lateral spacing, and 10 degree of inclination angle in the case of 3 m of excavation depth. Additionally, inclined potential failure surface occurs with approximately 60 degrees from the end of nails and the surface settlement and wall lateral displacement are restrained successfully by the large diameter soil nailing, based on the result of shear strain rate.

Fermentation of Cucurbita maxima Extracts with Microganisms from Kimchi (김치 유래 유산균을 이용한 단호박 발효음료 제조 기술 개발)

  • Roh, Hyun-Ji;Kim, Gi-Eun
    • KSBB Journal
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    • v.24 no.2
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    • pp.149-155
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    • 2009
  • 19 strains, which could be identified as Lactobacillus sp. were isolated. The Cucurbita maxima has been known as a traditional healthy food and variable positive effects on the human body were already reported. In this study we tried to develop a production process for a healthy fermented drink with Cucurbita maxima and strains originated from Kimchi. Many kinds of lacctobacci species existed in the fermented food cannot survive in the acidic conditions in the stomach. So we tried to search and select a strain, which can arrive to the small intestine. A species of a Lactobacillus named as C332 was identifed as Lactobacillus plantarum and selected for the fermentation process. With the treatment with artificial gastric juice and artificial bile the survival rate of the cells could be calculated. The physiological characteristics at the variable conditions have been tested. After fermentation process the sensoric tests on the product with panels were tried. The most of the cells could survive in the acidic conditions and falcultive anaerobe. Especially some antibacterial effects aganinst E.coli were also found. With all kinds of the results from our research the fermented Cucurbita maxima drink can be a successful item in the market.

Electrochemical Characteristics of Ru Added Li4Ti5O12 as an Anode Material (Ru를 첨가한 음극활물질 Li4Ti5O12의 전기화학적 특성)

  • Cho, Woo-Ram;Na, Byung-Ki
    • Clean Technology
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    • v.20 no.4
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    • pp.433-438
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    • 2014
  • There is an increasing interest in the development of rechargeable batteries suitable for use in both hybrid electric vehicles and energy storage systems that require higher charge & discharge rates, bigger battery sizes and increased safety of the batteries. Spinel-type lithium titanium oxide ($Li_4Ti_5O_{12}$) as a potential anode for lithium ion batteries has many advantages. It is a zero-strain materials and it experiences no structural change during the charge/discharge precess. Thus, it has long cycle life due to its structural integrity. It also offers a stable operation voltage of approximately 1.55 V versus $Li^+/Li$, above the reduction potential of most organic electrolyte. In this study, Ru added $Li_4Ti_5O_{12}$ composites were synthesized by solid state process. The characteristics of active material were investigated with TGA-DTA, XRD, SEM and charge/discharge test. The capacity was reduced when Ru was added, however, the polarization decreased. The capacity rate of $Li_4Ti_5O_{12}$ with Ru (3%, 4%) addition was reduced during the charge/discharge precess with 10 C-rate as a high current density.

Structural Analysis of the Dual Thickness Laser Welded Frame (이종두께 레이저 용접 프레임의 구조해석)

  • 이영신;윤충섭;오재문
    • Computational Structural Engineering
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    • v.10 no.4
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    • pp.165-175
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    • 1997
  • In this paper, the stress, buckling and vibration analyses have been performed for several case with the spot weld stiffened rear side frame, the unstiffened rear side frame and the dual thickness laser weld rear side frame. For stress and vibration analyses, the clamped boundary condition with spring supports are used. But for the buckling analyses, the both ends simply supported boundary conditions are used. For the nummerical analyses, ANSYS 5.0 code is adopted. Maximum stress of the spot weld stiffened rear side frame occurs in the main frame and is 80.9 MPa. Maximum strain is 501 .mu.. The maximum stress of the dual thickness laser weld rear side frame of 1.8mm thickness structure is equal with the stress of spot weld stiffened frame. The weight of dual thickness laser weld frame can be reduced about 17.2%. For the stiffened spot weld rear side frame with both ends simply supported boundary conditon, the bucking load is 52.54 kN. When the thickness of the dual thickness laser weld rear side frame become 1.9mm thickness structure, the buckling load of the stiffenerd rear side frame is equal to that of dual thickness laser weld frame. The reduction of the structure weight is about 5%. The fundamental natural frequency of the stiffened spot weld rear side frame for bending mode is 163.6 Hz and that of the dual thickness laser weld rear side frame is 179.8 Hz.

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