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Effects of laser-irradiated dentin on shear bond strength of composite resin (레이저 처리가 상아질과 복합 레진의 결합에 미치는 영향)

  • Kim, Sung-Sook;Park, Jong-Il;Lee, Jae-In;Kim, Gye-Sun;Cho, Hye-Won
    • The Journal of Korean Academy of Prosthodontics
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    • v.46 no.5
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    • pp.520-527
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    • 2008
  • Purpose: This study was conducted to evaluate the shear bond strength of composite resin to dentin when etched with laser instead of phosphoric acid. Material and methods: Recently extracted forty molars, completely free of dental caries, were embedded into acrylic resin. After exposing dentin with diamond saw, teeth surface were polished with a series of SiC paper. The teeth were divided into four groups composed of 10 specimens each; 1) no surface treated group as a control 2) acid-etched with 35%-phosphoric acid 3) Er:YAG laser treated 4) Er,Cr:YSGG laser treated. A dentin bonding agent (Adapter Single Bond2, 3M/ESPE) was applied to the specimens and then transparent plastic tubes (3 mm of height and diameter) were placed on each dentin. The composite resin was inserted into the tubes and cured. All the specimens were stored in distilled water at $37^{\circ}C$ for 24 hours and the shear bond strength was measured using a universal testing machine (Z020, Zwick, Germany). The data of tensile bond strength were statistically analyzed by one-way ANOVA and Duncan's test at ${\alpha}$= 0.05. Results: The bond strengths of Er:YAG laser-treated group was $3.98{\pm}0.88$ MPa and Er,Cr:YSGG laser-treated group showed $3.70{\pm}1.55$ MPa. There were no significant differences between two laser groups. The control group showed the lowest bond strength, $1.52{\pm}0.42$ MPa and the highest shear bond strength was presented in acid-etched group, $7.10{\pm}1.86$ MPa (P < .05). Conclusion: Laser-etched group exhibited significantly higer bond strength than that of control group, while still weaker than that of the phosphoric acid-etched group.

Effects of Soybean and DJI Chungkukjang Powder on Blood Glucose and Serum Lipid Reduction in db/db Mice (대두 및 DJI 청국장 분말이 db/db 마우스의 혈당과 혈청 지질 감소에 미치는 영향)

  • Lee, Jae-Joon;Kim, Ah-Ra;Chang, Hae-Choon;Jung, Hae-Ok;Lee, Myung-Yul
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.8
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    • pp.1086-1093
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    • 2012
  • The hypoglycemic and hypolipidemic effects of autoclaved soy flour and DJI chungkukjang powder fermented using Bacillus subtilis DJI were investigated in type 2 diabetic animal models. After a 2-week adaptation period, the diabetic animal model db/db mice were divided into the diabetic control group (D-C group), a diabetic group fed with soybean (D-S group), and a diabetic group fed with DJI chungkukjang (D-CJ group). The body weight gain, food intake, water intake, liver, and adipose tissue weights were not significantly different between the experimental groups. The supplementation of DJI chungkukjang or autoclaved soy flour diet induced a marked reduction of fasting blood glucose, blood glycosylated hemoglobin levels, and glucose levels in the oral glucose tolerance test and AUC for glucose compared with the diabetic control group. However, DJI chungkukjang showed a much stronger antidiabetic effect than unfermented autoclaved soy flour. Serum insulin levels were the same among the groups. The supplementation of DJI chungkukjang or autoclaved soy flour diet also significantly lowered the serum triglyceride, total cholesterol, and LDL-cholesterol levels compared with the control diabetic group, while it elevated the HDL-cholesterol level in the serum. This data suggests that the dietary supplementation of autoclaved soy flour or DJI chungkukjang may be useful in the control of blood glucose in animals with type 2 diabetes.

A Comparative Study on the Effects of Wearing Reverse Geometry Lenses by Degrees of Myopia (근시정도에 따른 역기하렌즈 착용효과에 관한 비교 연구)

  • Yoon, Min-Hwa;Lee, Ki-Young
    • Journal of Korean Ophthalmic Optics Society
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    • v.17 no.3
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    • pp.265-272
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    • 2012
  • Purpose: To compare the results on myopia correction with reverse geometry lenses, effects of wearing reverse geometry lenses were evaluated for the children with low-level and high-level myopia. Methods: The research investigated the corrective effects of having worn reverse geometry lenses for one week, one month, three months and six months on a total of thirty-six persons (sixty-two eyes) between the ages of seven and fifteen, divided into three groups by the degree of their myopia; nineteen eyes(Group One) with myopia of -2.00 D and under, twenty-eight eyes(Group Two) with myopia between -2.25 D and -4 D, and fifteen eyes(Group Three) with myopia of -4.25 D and above; as shown by changes in uncorrected vision and the degree of refraction in the corneal topography, and tested for statistical similarity among the pursued results. Results: After wearing reverse geometry lenses, Group One showed an improvement in vision of 0.5, from 0.45 to 0.95, after one week, and improvements to 0.91 after one month and 1.02 after three months but, after six months, the group's vision regressed to 0.95. Group Two showed an improvement in vision of 0.43, from 0.34 to 0.77, after one week of wearing and to 0.91 after one month, to 0.97 after three months and this was statistically maintained through the remainder of six months. Group Three showed an improvement in vision of 0.55, from 0.15 to 0.7, after wearing for one week, to 0.87 after one month and to 0.91 after three months but saw a regression to 0.86 after six months. The average Sim K (simulated keratometry reading) value for Group One started from $42.84{\pm}1.17D$ and decreased to $41.48{\pm}0.98D$ after one week of wearing and continued declining through three months before increasing during the remainder of six months. Group Two began from $42.91{\pm}1.57D$ and recorded $41.78{\pm}1.58 D$ after one week, continuing the decline through three months before increasing during the remainder of six months. Group Three began at $42.64{\pm}1.64D$ and showed its Sim K value decrease to $40.77{\pm}1.20D$ after one week of wearing, increase after one month and decrease after three months and continue the decline through the remainder of six months. Conclusions: From the results of this study, wearing reverse geometry lenses had myopia-correcting effects after one week of wearing. Although there were variations in the time for such effect to take place but myopia-correcting effects were evident in all test groups.

Controlled Release of 2,4-D(2,4-Dichlorophenoxy Acetic Acid ) from the Complex of Rice Husk Lignin and 2,4-D-IV. Variation of Herbicidal Activity by Soil Environmental Factors (조곡(組穀) Lignin과 2,4-D (2,4-Dichlorophenoxy Acetic Acid) 결합체(結合體)의 방출제어(放出制御) 연구(硏究)- IV. 토양환경요인(土壤環境要因)에 따른 제초활성(除草活性)의 변이(變異))

  • Guh, J.O.;Lee, D.J.;Lim, K.P.;Kwon, S.L.
    • Korean Journal of Weed Science
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    • v.10 no.2
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    • pp.114-121
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    • 1990
  • Inactivation in soil absorption, translocation of 2, 4-D by plants vary depending upon soil environments and herbicide formulations. Experiment was conducted in a glasshouse using rectangular pots($1350cm^2$) to evaluate the growth responses of barnyardgrass (Echinochloa crus-galli) and Indian jointvetch (Aesehyrcomene indica) to two formulations of 2, 4-D. The formulations used were 40% 2, 4-D amin salt (2, 4-D/AS) and 19.7% complex of rice husk lignin and 2, 4-D (2, 4-D/LG) which were applied at 200g ai/ha. Soil environments included fertilizer levels, soil pH, organic matter contents, and soil textures, Each treatment was replicated three times. The herbicidal activity of 2.4-D increased and lasted with increased levels of fertilizer. The activity also increased and lasted with low soil pH and decreased content of organic matter. Generally 2, 4-D/LG showed higher and longer herbicidal activity than 2. 4-D/AS for both test plants under all conditions applied. However, the herbicidal activity was influenced by the formulations more than by soil textures. It was thought that 2, 4-D/AS was released in a short time and inactivated readily while 2, 4-D/LG was slowly released and gave an opportunity of absorption by plants for a long period.

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Identification of Lettuce Germplasms and Commercial Cultivars Using SSR Markers Developed from EST (EST로부터 개발된 SSR 마커를 이용한 상추 유전자원 및 유통품종의 식별)

  • Hong, Jee-Hwa;Kwon, Yong-Sham;Choi, Keun-Jin;Mishra, Raghvendra Kumar;Kim, Doo Hwan
    • Horticultural Science & Technology
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    • v.31 no.6
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    • pp.772-781
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    • 2013
  • The objective of this study was to develop simple sequence repeat (SSR) markers from expressed sequence tags (EST) of lettuce (Lactuca sativa) and identify 9 germplasms from 3 wild species of lettuce and 61 commercial cultivars using the developed EST-SSR markers. A total of 81,330 lettuce ESTs from NCBI databases were used to search for SSR and 4,229 SSR loci were identified. The highest proportion (59.12%, 2500) was represented by trinucleotide, followed by dinucleotide (29.70%, 1256) and hexanucleotide (6.62%, 280) among SSR repeat motifs. Totally 474 EST-SSR primers were developed from EST and a random set of 267 primers was used to assess the genetic diversity among 9 germplasms and 61 cultivars. Out of 267 primers, 47 EST-SSR markers showed polymorphism between 7 cultivars. Twenty-six EST-SSR markers among 47 EST-SSR markers showed high polymorphism, reproducibility, and band clearance. The relationship between 26 markers genotypes and 70 accessions was analyzed. Totally 127 polymorphic amplified fragments were obtained by 26 EST-SSR markers and two to nine SSR alleles were detected for each locus with an average of 4.88 alleles per locus. Average polymorphism information content was 0.542, ranging from 0.269 to 0.768. Genetic distance of clusters ranged from 0.05 to 0.94 between 70 accessions and dendrogram at a similarity of 0.34 gave 7 main clusters. Analysis of genetic diversity revealed by these 26 EST-SSR markers showed that the 9 germplasms and 61 commercial cultivars were discriminated by marker genotypes. These newly developed EST-SSR markers will be useful for cultivar identification and distinctness, uniformity and stability test of lettuce.

The Composition of the Root of Ixeris dentata var. albiflora Nakai. and Cell Viability and DPPH Radical Scavenging Activities of its Extract (흰씀바귀 (고채(苦菜), Ixeris dentata var. albiflora Nakai)뿌리의 성분 분석과 추출물의 세포 생존율 및 DPPH 라디칼 소거 활성)

  • Hong, Seul-Gi;Jeong, Dong-Myong;Kim, Ki-Young;Hwang, Eun-Hee
    • Journal of Nutrition and Health
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    • v.43 no.2
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    • pp.105-113
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    • 2010
  • Ixeris dentata var. albiflora Nakai, a herbal plant, is often used to make a strong stomach as an antiphlogistic used when dyspepsia and to improve appetite in Korea and China. And also it is used for adult diseases such as diabetes and liver diseases as Korean traditional medicine. In this study, the composition and DPPH radical scavenging activities of the root of Ixeris dentata var. albiflora Nakai and its effects on cell viability on vero and chang cells were investigated. Moisture, crude ash, crude protein and crude lipid were 79.14, 2.49, 8.28 and 2.56 g/100 g respectively. The highest mineral content was K. The major free sugars were glucose, fructose and sucrose. Major fatty acid are linoleic acid, palmic acid and linolenic acid. Major amino acids were glutamic acid, arginine and aspartic acid and the total contents of amino acids were 28.12 mg/g. The methanol extracts were further fractionated with n-hexane, chloroform, ethylacetate, butanol and water to get an active fraction. In addition, cell viabilities in each fraction were determined. Methanol extract, butanol, and aqueous fraction showed strong survival rates in vero cell and chang cell viability test, and hexane, chloroform, and ethylacetate fraction were examined for toxin in a cell. The root of Ixeris dentata var. albiflora Nakai had scavenging activities against DPPH radicals in a dose-dependent assay. Ethylacetate fraction's SC50 was $6.8\{\mu}g/mL$, very strong DPPH radical scavenging activities, but water fraction did not show any activity.

Effects of Isoflavone Supplementation on Lipid Profiles and Antioxidant Systems in Rats Fed with Cholesterol Diet (고콜레스테롤 식이를 섭취한 흰쥐에서 이소플라본 보충이 혈청 지질패턴 및 항산화체계에 미치는 영향)

  • Kim, Soo-Yeon;Kim, Soon-Young;Chung, Chung-Eun;Yoon, Sun;Park, Jung-Hwa
    • Journal of Life Science
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    • v.20 no.11
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    • pp.1683-1690
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    • 2010
  • There is an increasing interest in the potential of isoflavone in reducing the risk of cardiovascular disease and cancer, however, although several effects of isoflavone as a component of soy protein are well established, the hypolipidemic and antioxidant effects of purified isoflavone are still controversial. This study was to investigate the effects of isoflavone on serum lipid profiles and antioxidant status in rats. 7-week old male Sprague Dawley rats were fed one of the following diets for 8 weeks: basal diet (B), basal+0.3% isoflavone (BI), basal+0.5% cholesterol (BC), or basal+0.3% isoflavone +0.5% cholesterol (BIC). Two-way ANOVA was used to test the effects of dietary isoflavone and cholesterol supplementation and their interaction on variables. Serum lipid profiles and total antioxidant status (TAS) were examined spectrophotometrically. Degree of serum lipid peroxidation was measured by malondialdehyde (MDA) assay. The activities of serum antioxidant enzymes (GSH-Px, total-SOD) was determined. Levels of serum total cholesterol, VLDL+LDL-cholesterol and Atherogenic index were significantly lower in BI than those levels in group B (p=0.0002, p<0.0001, and p=0.0042, respectively). Serum total antioxidant status (TAS) levels were significantly higher, in both isoflavone supplemented groups (BI, BIC) compared to those levels in each control group (B, BC) (p<0.0001). Activity of total-SOD was significantly higher in BI compared to the activities in group B (p=0.0317). There was no interaction between isoflavone and cholesterol supplementation. In conclusion, isoflavone supplementation showed positive effects on the serum lipid profiles and total antioxidant activities in both conditions, either when fed a diet with or without cholesterol. These effects of isoflavone were independent of cholesterol supplementation.

Thermal Property and Fire Resistance of Cellulose Insulation (섬유질 단열재의 열적 특성 및 내화성능)

  • Kwon, Young-Cheol;Seo, Seong Yeon;Kim, Sung Young
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.9 no.3
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    • pp.203-212
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    • 2005
  • Cellulose insulation is primarily manufactured from recycled newsprint and treated with fire retardants for the fire resistance. Thanks to the fire retardants, it is not combustible and flammable. In addition to that, Its thermal resistance is much better than that of fiberglass or rock wool. It is made from waste paper and easily decayed when it is demolished, and it has small embodied energy. So it is very environment-friendly building material. For broader use of cellulose insulation in buildings in Korea, it is necessary to test its physical performance to compare the results with the requirements on the Korean Building Code. To this end, apparent thermal conductivity (ka) measurements of Korean-made loose-fill cellulose insulations were recently completed using equipment that was built and operated in accordance with ASTM C 518 and the fire resistance was tested in accordance with ASTM C 1485. Korean loose-fill cellulose has thermal conductivity about 5% greater than the corresponding U.S. product at the same density. This is likely due to differences in the recycled material being used. Both spray-applied and loose-fill cellulose insulation lose about 1.5% of their thermal resistivity for $5.5^{\circ}C$ increase in temperature. The fire resistance of cellulose insulation is increased in linear proportion to the increase of the rate of fire retardant. Thanks to the high fire resistance, cellulose insulation can be used as a substitution of Styrofoam or Urethane foam which is combustible. The thermal conductivity of cellulose insulation was $0.037-0.043W/m{\cdot}K$ at the mean specimen temperature from $4-43^{\circ}C$. It corresponds to the thermal resistance of "Na Grade" according to the Korean Building Code. The effect of chemical content on thermal conductivity was negligible for all but the chemical-free specimen which had the highest value for the thermal conductivity over the temperature range tested. The thermal resistance of cellulose insulation is better than that of fiberglass or rock wool, and its fire resistance is higher than that of Styrofoam or Urethane foam. Therefore it can be substituted for those above considering its physical performance. Cellulose insulation is no more expensive than Styrofoam or rock wool, so it is recommended to use it more widely in Korea.

Distribution of the Korean Dental Hygiene Research Agenda Suggested by the Korean Dental Hygienists Association and Affiliated Groups (대한치과위생사협회 및 산하단체에서 제안한 한국 치위생학 연구의제 분포)

  • Lee, Ga-Yeong;Lee, Sun-Mi;Kim, Chang-Hee;Bae, Soo-Myoung;Kim, and Nam-Hee
    • Journal of dental hygiene science
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    • v.16 no.5
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    • pp.393-399
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    • 2016
  • The objective of this study was to realize research demands by gathering the research agenda from the Korean Dental Hygienists Association and affiliated groups through a Delphi survey. The study subjects were the heads of eight groups. The Delphi survey was conducted by e-mail after a researcher explained the objective of the study to the heads. The research agenda was classified according to the Korean dental hygiene academic classification and the National Dental Hygiene Research Agenda classification. To compare the number of research agendas according to classification among the groups, we conducted a chi-squared test by using IBM SPSS 20.0. In total, 316 agendas were submitted. According to the Korean dental hygiene academic classification, there was a significant difference among the groups, and the agendas associated with clinical dental hygiene (36.7%) were the best. According to the National Dental Hygiene Research Agenda classification, there was no significant difference and the agendas associated with health promotion and disease prevention (27.8%) were the best. In conclusion, the Korean Dental Hygienists Association and affiliated groups demanded similar research agendas, corresponding to the purpose of each establishment. However, the groups did not seem to show unique characteristics according to the purpose of each establishment. In other words, the groups should corroborate such characteristics and suggest a corresponding agenda to establish specific areas of research in dental hygiene. Also, dental hygiene researchers should mark the academic classification of the studies they want to submit.

Seedling Emergence and Growth Affected by Priming and $GA_3$ Treatments to Three Campanulan Plant Seeds (Priming과 $GA_3$ 처리에 따른 도라지, 더덕 및 만삼의 출아(出芽)와 생장(生長))

  • Kang, Jin-Ho;Kim, Dong-Il;Kang, Shin-Yun;Shim, Young-Do;Han, Kyung-Soo
    • Korean Journal of Medicinal Crop Science
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    • v.5 no.4
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    • pp.307-313
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    • 1997
  • Seed germination test done in laboratory does not coincide with field emergence in general. The experiments were carried out to examine the effect of priming and $GA_3$, treatment to seeds of Platycodon grandiflorum; Codonopsis lanceolata and C. pilosula on lapsed time to first seedling emergence, seedling emergence, morphological characters and growth and the cause of poor emergence of C. pilosula. No-treatment as Control (water), priming or $GA_3$ treatment was done with only distilled water for 2 days, $CA(NO_3)_2$ 150 mM for 2 days or $GA_3$ 0.1 mM for 3 days, respectively. Seedling emergence rate was counted every 2 days but morphological characters and dry weight of shoot and root were measured on 38 days after sowing. Their internal seed structures were examined with Scanning Electron Microscope. C. pilosula had poorer seedling emergence rate than P grandiflorum and C. lanceolata showing nearly same rate: Compared to the other treatment (s) P. grandiflorum displayed higher rate in priming and $GA_3$, treatments but C. lanceolata or C. pilosula did the greatest rate in only $GA_3$ or priming treatment, respectively. $GA_3$ treatment to seeds of P. grandiflorum and C. lanceolata shortened the lapsed time to seedling emergence in comparison with Control, 2-days water imbibition before sowing. In all the species plant height and number of leaves per seedling became shorter and less in priming treatment than the other treatments except plant height of C. Pilosula while their hypocotyl length was nearly same in all treatments. Although priming treatment had nearly similar effect to morphological characters, $GA_3$ treatment forced greater shoot, root and aftermath total dry weight per seedling. Poor seedling emergence of C. pilosula was caused by its seed defect like cleavage or lack of embryo, poor development of embryo and endosperm or their separation.

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