• 한국약용작물학회:학술대회논문집
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• 2010.10a
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• pp.505-506
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• 2010

• Journal of the Korean Applied Science and Technology
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• v.38 no.1
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• pp.136-146
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• 2021

This study is a descriptive research to determine the degree of self-esteem, menopausal symptom, spousal support of middle-aged women and investigate the factors affecting aging anxiety. For 3 weeks from May 23 to June 13, 2020, data total of 110 people were collected using online questionnaires for late middle-aged women. As a result of date analysis, factors affecting aging anxiety in late middle-aged women were menopausal symptoms(B=17.943, p<.001), self-esteem(B=-.585, p=.001) and the explanatory power of the regression model was 41.4%. Therefore, in order to lower againg anxiety, active intervention for menopausal symptoms of late middle-aged women is required, and measures should be taken to increase self-esteem.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.117-117
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• 2019

Kirengeshoma koreana Nakai (K. koreana)was Saxifragaceae and rare plants in Korea, which is classified as an Critically Endangered (CR) species in Korea. Therefore, most of the studies on it were ecological and taxonomic, and there are no studies on biological activity. In this study, we evaluated the whitening activity of K. koreana extract (KKE). Melanogenesis Inhibitory effects were demonstrated by western-bot and RT-PCR for the effects of KKE on MITF, tyrosinase, TRP-1 and TRP-2 in IBMX-treated B16F10 melanoma cells. IBMX were reported as melanin synthesis enhancers. It could increase intracellular melanin synthesis by activation of the microphthalmia-associated transcription factor (MITF) signaling pathway. KKE showed no cytotoxicity at B16F10. In addition, KKE effectively inhibited the protein and mRNA levels of MITF, tyrosinase, TRP-1 and TRP-2. In conclusion, KKE inhibited melanin synthesis by inhibiting the expression of MITF and its downstream pathways tyrosinase, TRP-1 and TRP-2. Therefore, it was confirmed that K. koreana is a valuable resource for functional cosmetic and biomaterials.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.53-53
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• 2023

Abeliophyllum distichum, one of the Korean endemic plant, is a significant pharmaceutical plant resource. A. distichum with phenylethanoid glycoside can use to regulate the development of cancer, DNA damage with radicals, and the generation of inflammatory mediators. In this study, we investigated whether the biomass, content of phenylethanoid glycoside, and growth rate of callus derived from A. distichum (cultivar Okhwang1, CAD) change in the absence or presence of plant hormones (2,4-Dichlorophenoxyacetic acid; 2, 4-D and 1-Naphthaleneacetic acid; NAA). The results showed that the best biomass, the growth rate of callus, and the contents of phenylethanoid glycoside were cultivated on Murashige and Skoog (MS) growth medium fortified with 1 ppm 2,4-D + 2 ppm NAA after 4 weeks. In a further study, CAD was cultivated on MS growth medium fortified with an elicitor (Methyl Jasmonate, MeJA). The results showed that CAD turned to brown color and fragile form with the elicitor. HPLC-PDA analysis revealed that the contents of phenylethanoid glycoside in the elicitor-treated group were higher than in the elicitor-non-treated group. These results are consistent with the findings of Arano-Varela H et al.,'s study which is that acteoside production can increase after the treatment of MeJA. Therefore, this study can be used to develop an effective and sustainable production of useful substances as an alternative to plant cultivation.

• Journal of Industrial Convergence
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• v.19 no.5
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• pp.129-137
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• 2021

The present study attempted to identify how the knowledge and risk perception of nursing students regarding COVID-19 impact COVID-19 preventive behaviors. This study collected data from May 1st to September 20th, 2021, using a self-reported questionnaire targeting 184 nursing students. The collected data were analyzed using Independent t-test, One-way ANOVA, and Multiple regression. The research results indicated that the knowledge(r=.15, p=.019) and risk perception(r=.13, p=.036) of nursing students regarding COVID-19 had a positive correlation with COVID-19 preventive behaviors. The factor that impacted the COVID-19 preventive behaviors of the subjects was COVID-19 knowledge, which had an explanatory power of 4%. Therefore, it is necessary to develop an educational intervention program that enables nursing students to obtain the correct knowledge regarding COVID-19 and improves preventive behaviors.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.53-53
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• 2021

Abeliophyllum distichum Nakai (A. distichum), endemic species of Korea, is classified according to the petals and calyx colors. Recently, A. distichum cv. Okhwang 1, which has the golden flower, designated the first official cultivar improved from A. distichum species. The study on the chloroplast genome of A. distichum cv. Okhwang 1 have been reported, but no studies on bioactivity such as antioxidant, anti-inflammatory, and anti-cancer have been progressed. This study was conducted to evaluate the inhibition on melanogenesis of the flower from A. distichum cv. Okhwang 1 (FAO). Antioxidant activity was measured using DPPH and ABTS radical scavenging assays. Inhibition effects on melanogenesis of FAO were confirmed by expression of tyrosinase-related proteins and mRNAs using immunoblotting and RT-qPCR. Tyrosinase is an enzyme that regulates both stimulation and inhibition of melanogenesis. Stimulated MITF in cellular levels increases the expressions of tyrosinase, TRP-1, and TRP-2 to induce melanogenesis. As a result, FAO inhibited the expression of MITF, followed by down-regulated tyrosinase, TRP-1, and TRP-2, which lead to inhibit melanin overproduction. In conclusion, these results indicated that FAO reduced reactive oxygen species (ROS) and markedly inhibited the expression of melanin-related factors. The present study suggested providing that FAO has the potential for development as a functional cosmetic material derived from natural.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.54-54
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• 2021

The Melanoma Research Coalition reported melanoma affects humans of various races. This study was conducted to confirm the inhibitory effect of melanogenesis in B16 F10 cells of Nypa fruticans Wurmb of ethyl acetate fraction (NEF). Nypa fruticans Wurmb is an important component of the East Asian mangrove vegetation. It belongs to Araceae family. Traditionally, N. fruticans was used to treat various diseases such as asthma, sore throat, liver disease, a pain reliever, and can also be used as sedative and carminative. The present study, the inhibitory effect on melanogenesis was determined by Western blotting and RT-qPCR. The level of expression of tyrosinase, TRP-1, and TRP-2 is regulated by microphthalmia-associated transcription factor (MITF) and cAMP, and cAMP affects the activity of protein kinase A (PKA). Activated PKA stimulates the phosphorylation of cAMP-reactive element-binding protein (CREB) in the nucleus, thereby increasing the amount of MITF expression and enhancing melanogenesis. Western blotting and RT-qPCR analysis showed that NEF treatment decreased the expression of tyrosinase. Similarly, TRP-1 and TRP-2 levels were decreased, which were decreased significantly at compared with the untreated control. Also, NEF attenuated the IBMX mediated increase in the intracellular cAMP level and the phosphorylation of PKA. In conclusion, NEF significantly inhibited the expressions of melanogenesis through cAMP/PKA/CREB signaling pathways.

• Journal of Industrial Convergence
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• v.20 no.5
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• pp.101-110
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• 2022

This study aims to understand the effects of nursing college students' anxiety about clinical practice and stress from it during the practice on their nursing professionalism during COVID-19 and be used as baseline data to promote this professionalism. The target is 187 juniors and seniors in nursing college located in Chungnam, Chungbuk, and Gyeongbuk with experience in the clinical practice, and the survey was conducted from September to November 2021. The data was analyzed by t-test, ANOVA, Pearson correlation coefficient, and multiple regression using SPSS 22.0. Nursing professionalism was significantly correlated with clinical practice stress(r=-.151, p=.039). In terms of the factors that influenced the nursing professionalism, the coefficient of determination of satisfaction in major was 5.2%(β=-.24, p=.001), and that of stress from the clinical practice was 3.0%(β=-.17, p=.015), which explains a total of 8.2%. Major satisfaction was the most influential factor predicting greater nursing professionalism. The results of this study demonstrate the necessity to increase nursing students' satisfaction in major and reduce their stress from clinical practice in order to improve the nursing professionalism during COVID-19.

• Journal of the Korean Applied Science and Technology
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• v.40 no.3
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• pp.521-533
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• 2023

The purpose of this study was to investigate the effect on the clinical competence by the face-to-face, non-face-to-face clinical practice stress and the stress coping. A survey was conducted among nursing students of university in Seoul and Chungcheong City from June 10 to July 10, 2021. 201 copies were included in the final data analysis, Pearson's correlation coefficient and hierarchical regressions was used. As a result, in the first stage, nursing students grades, major satisfaction, and face-to-face practice satisfaction explained 19.4% of their clinical performance ability, in the second step, stress coping was added to increase explanatory power by 19.6% allowing a total of 39.0% of randomness to be explained. Therefore, this study could be used as a basic data for the counseling, development, and education programs for stress coping to increase clinical competence.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.118-118
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• 2019

Abeliophyllum distichum Nakai belonging to Oleaceae is only species in Korean endemic genus, Abeliophyllum. Abeliophyllum distichum (AD) is divided into various types according to flower shape and color. AD is known to have various colors such as white, pink, and ivory. Recently, light yellow flowers have been registered as new varieties (Okhwang 1ho). To date, various ecological and morphological studies on AD have been carried out, but no studies have been made on the phytochemicals and activities according to various traits. In this study, we analyzed the phytochemicals and antioxidative activities of from four kinds of flowers (white, pink, ivory, light yellow) in full bloom. The contents of phytochemicals such as chlorogenic acid, Hirsutrin, Rutin, Acteoside and Isoacteoside were analyzed by HPLC. Antioxidant activity was evaluated by DPPH, ABTS. As a result, the content of each substance varied according to the flower color. These results will provide basic data for evaluating the usefulness of genetic resources in Korea and developing new functional materials in preparation for the Nagoya Protocol.