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연취급 근로자들의 생화학적 검사에 관한 연구

  • 김준연
    • 산업보건소식
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    • 제23호
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    • pp.24-31
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    • 1985
  • 연 취급 근로자 236명과 대학생 120명을 대상으로 연 폭로에 관련되는 생물학적인 지표들의 농도를 측정한 결과 PbB.Hb.PbU.ALAU에서 양군간에 유의한 차이를 시현하였으며, 연 근로자에서 PbU 및 CPU가 PbB의 증가에 따라 점증 경향을 보였다.

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Characterizations of the bovine subtype Interferon-tau Genes : Sequences of Genes and Biological Activity of Transcription Factors in JEG3 Cell

  • Kim, Min-Su;Min, Kwan-Sik;Seong, Hwan-Hoo;Kim, Chan-Lan;Kim, Dongkyo;Imakawa, Kazuhiko;Kim, Sung Woo
    • 한국수정란이식학회지
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    • 제31권4호
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    • pp.335-347
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    • 2016
  • Multiple interferon tau (IFNT) genes exist in bovine. An antiluteolytic substance secreted by the bovine conceptus and primarily responsible for maternal recognition of pregnancy is bovine trophoblast protein 1 (bIFNT1), a new type I interferon tau (IFNT) genes. The objectives of this research were to investigate whether multiple, distinct gene encode bIFNT1 and other type I bIFNT gene in the bovine genome and to examine expression of bIFNT1 and other bIFNTc1 mRNAs during conceptus development. These transcrips could be regulated through caudal-related homeobox-2 (CDX2) and ETS2 and/or AP1 (JUN) expression, a transcription factor implicated in the control of cell differentiation in the trophectoderm. The presence of mRNAs encoded by bIFNT1 and type I bIFNTc1 genes were examined quantitatively via reverse transcription-polymerase chain reaction (RT-PCR) analysis of total cellular RNA (tcRNA) extracted from on day 17, 20 and 22 bovine conceptuses. The expression level of bIFNT1 was higher on day 17 transcripts were gradually weakly detectable on day 20 and 22. However, the other bIFNTc1 gene examined transcripts was highly expressed on day 20 and transcripts were weakly detectable on day 17 and 22 bovine conceptuses. Furthermore, human choriocarcinoma JEG3 was co-transfected with an -1kb-bIFNT1/c1-Luc constructs and several transcription factor expression plasmids. Compared to each -1kb-bIFNT1/c1-Luc increased when this constructs were co-transfected with, ETS2, AP1(JUN), CREBBP and/or CDX2. Also, bIFNTc1 gene was had very effect on activity by alone ETS2, and AP1 (JUN) expression factors in choriocarcinoma JEG3 cell. However, bIFNT1 gene expression of the upstream region was not identified. We demonstrated that the activities of bIFN genes are regulated by differential, tissue-specific and developmental competence during pregnancy.

기계적 합금화 및 in-situ와 ex-situ의 혼합공정을 통한 C 도핑된 ex-situ $MgB_2$ 선재 제조 (Mechanical Alloying and Combined Process of in-situ and ex-situ to Fabricate the ex-situ C-doped $MgB_2$ Wire)

  • 황수민;이창민;임준형;최준혁;박진현;주진호;전병혁;김찬중
    • Progress in Superconductivity
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    • 제10권2호
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    • pp.79-86
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    • 2009
  • We successfully fabricated C-doped ex-situ $MgB_2$ wires using two different methods such as mechanical alloying(MA) and combined process(CP) of in-situ and ex-situ. In the MA, the precursor powder was prepared with a mixture of $MgB_2$ and 1 at% C powders by planetary ball milling for 0-100 h. In the CP, on the other hand, C-doped $MgB_2$ powder was prepared with Mg, B, and C powders by in-situ process via compaction, sintering, and crushing. The powders prepared by two methods were loaded into Fe tube and then the assemblages were drawn by a conventional powder-in-tube technique. The MA treatment of C-added $MgB_2$ decreased the particles/grains size and resulted in C-doping into $MgB_2$ after sintering, improving the critical current density($J_c$) in high external magnetic field. For the C-doped $MgB_2$ wire by MA for 25 h, the $J_c$ was $4.1{\times}10^3A/cm^2$ at 5 K and 6.4 T, which was 5.9 times higher than that of pure and untreated $MgB_2$ wire. The CP also provided C-doping into $MgB_2$ and improved the $J_c$ in high magnetic field; the C-doped $MgB_2$ wire fabricated by CP exhibited a $J_c$ being 2.3 times higher than that of the ex-situ wire used commercial $MgB_2$ powder at 5 K and 6.0 T($2.7{\times}10^3A/cm^2\;vs.\;1.2{\times}10^3A/cm^2$).

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EfficientNet 활용한 딸기 병해 진단 서비스 (Strawberry disease diagnosis service using EfficientNet)

  • 이창준;김진성;박준;김준영;박성욱;정세훈;심춘보
    • 스마트미디어저널
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    • 제11권5호
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    • pp.26-37
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    • 2022
  • 본 논문에서는 시설재배 작물 중 딸기의 초기 병해를 방제하고자 이미지를 자동으로 취득하고, EfficientNet 모델을 활용해 병해를 분석하여 농민에게 병해 여부를 알려주고, 전문가를 통한 병해 진단 서비스를 제안한다. 딸기 생육단계의 이미지를 취득하고, 학습된 EfficientNet 모델을 활용해 병해 진단 분석결과를 농민의 애플리케이션으로 전송 후 전문가의 피드백을 신속하게 받을 수 있다. 데이터 세트로는 실제 시설재배를 운영하는 농민을 섭외하여 시스템을 이용해 이미지를 취득하였고, 핸드폰으로 촬영한 이미지의 초안을 활용하여 데이터가 부족한 문제를 해결했다. 실험 결과 EfficientNet B0부터 B7까지의 정확도는 유사하여 추론 속도가 가장 빠른 B0를 채택했다. 성능향상을 위해 ImageNet으로 사전학습 된 모델을 사용해 Fine-tuning 했고, 100 Epoch부터 급격한 성능향상을 확인했다. 제안하는 서비스는 초기 병해를 빠르게 탐지하여 생산량을 증대시킬 것으로 기대한다.

SKEW COPAIRED BIALGEBRAS

  • Park, Jun Seok;Cho, Myung Sang
    • 충청수학회지
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    • 제16권1호
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    • pp.81-96
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    • 2003
  • Let ${\sigma}:k{\rightarrow}A{\otimes}B$ be a skew copairing on (A, B), where A and B are Hopf algebras of the same dimension n. Skew dual bases of A and B are introduced. If ${\sigma}$ is an invertible skew copairing then we can give a 2-cocycle bilinear form [${\sigma}$] on $A{\otimes}B$ and define a new Hopf algebra.

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B2B에서 구매의도 파악을 위한 웹 로그 분석 (Web Log Analysis for Studying the Intend to Purchasing Under B2B Environment)

  • 고재문;서준용;김운식
    • 한국경영과학회:학술대회논문집
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    • 한국경영과학회/대한산업공학회 2005년도 춘계공동학술대회 발표논문
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    • pp.601-613
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    • 2005
  • 일반적으로 B2C가 불특정 다수에 대한 서비스라면 B2B는 특정 소수에 대한 서비스라고 할 수 있다. 이러한 특성으로 B2C와 B2B에서 고객의 구매의도는 다르게 평가되어야 한다. 또한 B2B는 협상이라는 단계가 있고, 이것은 B2C와 B2B의 구매의도 평가기준에 영향을 미치게 된다. 본 연구에서는 B2B에서 구매의도 파악을 위한 웹 로그 분석 모형을 제시한다. 제시된 모형을 통해 구매의도 파악을 위한 웹 로그 분석 데이터를 추출하고, 추출된 데이터를 기업의 레거시 시스템 데이터와 통합하는 과정을 보여준다. 또한 분석 데이터를 추출하기 위한 웹마이닝 과정과 추출된 분석 데이터가 데이터베이스에 저장되는 과정을 보여준다.

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백화사설초 메탄올 추출물에 의한 HL-60 세포고사과정에 있어서의 transcriptional factors 활성변화 연구 (Study on Transcriptional Factors Activation Change of HL-60 cell Apoptosis by Hedyotis Diffusa's Methanol Extract)

  • 박상구;이지현;문구;문석재;원진희;박래길
    • 대한한방종양학회지
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    • 제6권1호
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    • pp.67-79
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    • 2000
  • Objective : Hedyotis diffusa has been used as an anticancer agent for several decades in oriental medicine. We test whether the methanol extract of the herb affects transcriptional activation factors including $NF-{\kappa}B$ and AP-1. Methods : 1. HL-60 cells were treated with various concentrations(from 200 to $50{\mu}g/ml$) of methanol extract and $H_2O$ extract($200{\mu}g/ml$)of hedyotis diffusa, After 48h later, the cells were tested for viability by MTT assay. 2. The HL-60 cells were treated with $200{\mu}g/ml$ of methanol extract for the indicated periods. First. Nuclear extracts were isolated and incubated with oligonucleotide probe of $NF-{\kappa}B$ and AP-1. Second. Nuclear extracts were isolated and reacted with p50, p65. c-rel pan-Jun, c-Jun, JunB. JunD antibody on ice for 30min. Finally The cell lysates were prepared and analyzed by western blotting using anti-Fas, anti-FasL and anti-p53 antibody. Results : 1. The methanol extract decreases the viability of human lymphoid origin leukemia HL-60 cells in a dose-dependent manner. 2. $NF-{\kappa}B$ is rapidly activated by the addition of the methanol extract, reaches a peak at 30min and gradually returns to resting level. We confirm that $NF-{\kappa}B$ is a heterodimer mainly composed of p65 subunit with c-Rel. 3. Transcriptional activation of AP-1 is detected at 30min and reaches a maximum at 1hr after stimulation of the cells with the methanol extract. AP-1 is mainly composed with Jur-D and partially Jug-B proteins. 4. the methanol extract of Hedyotis diffusa induces the expression of Fas, Fas ligand and p53 proteins of HL-60 cells in a time dependent fashion. Conclusions : These results suggest that the methanol extract of Hedyotis diffusa exerts anticancer effects to induce the death of human leukomic HL-60 cells via activation of trascriptional factors such as $NF-{\kappa}B$ and AP-1, increase in expression of Fas mediated signalling proteins, and induction of tumor suppressor gene. p53.

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Bifidobacterium sp.로 제조된 반죽의 물성적 특성 (Rheological Characteristics of Wheat Flour Dough with Bifidobacterium sp.)

  • 안덕준;홍정훈
    • 한국식생활문화학회지
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    • 제17권2호
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    • pp.165-170
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    • 2002
  • In order to economically utilize dough with B. longum, B. infantis and B. brevis as a bread improver, aerotolerance, ${\alpha}-galactosidase$ activity, organic acids, farinograph and extensograph of dough were investigated. In aerotolerance of Bifidobacterium sp., B. longum was highest among tested starters, followed by B. infantis. The ${\alpha}-galactosidase$ activity was highest in the B. longum among tested starters. In organic acids, the contents of lactic acid and acetic acid were the highest in the among tested starters, followed by B. infantis. In farinograms of dough, water absorption and peak time were highest in the B. brevis among tested dough. Extensogram showed that the area increased remarkably in B. longum and B. infantis at 135min of fermentation. Extensibility and resistance to extension of dough were highest in the B. infantis among the dough, followed B. longum.