• Asian Pacific Journal of Cancer Prevention
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• v.16 no.4
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• pp.1443-1448
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• 2015

To investigate the significance of FOXO3a and Ki67 in human lung adenocarcinomas. Envision immunohistochemical staining and Western blotting were used to examine the protein expression of FOXO3a in 127 cases of human lung adenocarcinoma specimens. The positive rate in lung adenocarcinoma (55.9%) was lower than that in normal tissues (80%). We found that the expression of FOXO3a was closely related with the degree of differentiation, TNM staging, lymph node metastasis and survival. In addition, significant differences in the different pathological types of lung adenocarcinoma cases (P<0.01). The FOXO3a positive rate of the acini as the main type (APA) (86.7%) and the lepidic as the main type (LPA) (82.4%) was higher than the solid as the main type (SPA) (50.0%), the papilla as the main type (PPA) (42.9%) and the micropapilla as the main type (MPA) (9.4%). Moreover, the expression of FOXO3a was negatively related with Ki67 expression. Our results suggested that the expression of FOXO3a is closely correlated with the aggressiveness of lung adenocarcinoma. It was indicated that disregulation of FOXO3a might play key roles in the occurrence and development of lung a denocarcinoma and joint detection of the two markers might play an important role in diagnosing tumors.

• The Journal of Korean Medicine
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• v.23 no.4
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• pp.98-104
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• 2002

Objectives: Calpain, a calcium-dependent cysteine proteinase, may be one of the proteolytic enzymes that mediate cartilage degradation associated with rheumatoid arthritis. The object of this study is to ascertain immunohistochemically whether calpain is present in the inflamed joints of collagen-induced arthritis of rats, and examine the effect of Cortex Acanthopanacis Senticosi on the expression of calpain. Methods: Male Lewis rats, around 200g of body weight, were immunized with bovine type II collagen. After 3 weeks from first immunization, rats were divided into arthritic control (n=6) group and Cortex Acanthopanacis Senticosi-treated (n=6) group. Non-immunized rats served as the normal (n=6) group. All animals were sacrificed at 15 days post-treatment and tibiotarsal joints were removed. Calpain immunohistochemistry was performed on the midsagittal section of the tibiotarsal joint. Results: All animals of the control and treated groups showed ankylosing osteoarthritis. However, the animals of the treated group showed alleviation in the fibrous ankylosis, destruction of articular cartilage and destruction of subchondral bony tissue compared with the animals of the control group. Calpain was expressed in the chondrocyte lacunae of growing articular cartilage, in the skeletal muscle fibers, in the peripheral nerves, and in the vessel walls around the joints of all groups. In the control and treated groups, calpain was also expressed in proliferating synovial epithelia, subsynovial stroma cells, surface of articular cartilage, and fibrous pannus around destructive subchondral bony tissue. However, the expression density of calpain in the treated group was diminished compared with the control group, especially in surface of articular cartilage and fibrous pannus. Conclusions: These observations indicated that calpain plays an important role in the destruction of cartilage and bone in collagen-induced arthritis of rats, and also indicated that Cortex Acanthopanacis Senticosi inhibits the development of arthritis by decreasing the expression of calpain.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.10
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• pp.733-738
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• 2017

In this study, we selected chlorine, a typical toxic material used in many workplaces, as the leakage material, and through the analysis of alternative scenarios based on the meteorological conditions in the summer frequently encountered in accidents, we suggest ways to improve the (method of analysis/accident scenario modeling). The analysis of 296 chemical accidents from January 2014 to December 2016 found that the highest rate of occurrence was in summer, accounting for 35.81% of the total. According to the risk assessment, the influence range and number of inhabitants in the influence area were 712.4 m and 20,090 under the annual mean weather conditions and 796.2 m and 27,143 people under the summer mean weather conditions, respectively. This result implies that, under certain conditions, the range of impacts in the current alternative scenario is incomplete. Therefore, risk assessment systems need to be improved in order to take into consideration the characteristics of each chemical substance.

• Journal of The Korean Association of Information Education
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• v.8 no.2
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• pp.177-187
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• 2004

It soaks but from 2003 the gifted child whom it is propelling as the enterprise of real national dimension oneself will know and it will do well the case which with the thought which goes wrong it lets to let alone is many but about lower social unsuitable Eung and melancholia, nervous characteristic anorexia cung with the back the same multi branch problem point occurs with emotional vulnerability of the gifted person. From the gifted people it grasps the emotional vulnerability which occurs from the dissertation which it sees consequently and the gifted person cyber counseling system which does the hazard web which solves a problem point in base plan and it embodies. One side, it applied the gifted child and the parents who are participating to an Dae-jeon 6th area joint the gifted person class in the gifted person cyber counseling system which it develops the result and it analyzed. Emotional vulnerability of the analysis result the gifted child considerable portion there is a possibility the fact that it overcomes, if facing each other it will be able to complement the portion which is insufficient from consultation.

• Journal of Acupuncture Research
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• v.19 no.2
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• pp.177-188
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• 2002

Objective : The purpose of this study is to investigate the immunohistological effect of Buthus martensi Karsch Herbal-acupuncture in treating the arthritis, performed several experimental items : those are paw edema, $IL-1{\beta}$, IL-6, IL-8, $TNF-{\alpha}$ and $PGE_2$. Methods : All the male Sprague Dawley rats used in this study were bred and maintaned in our pathogen-free rat colony and were 8 weeks of age at the start of to experiment. The experimental model of arthritis was induced by injection of $50{\mu}g/{\mu}{\ell}$ adjuvant(mineral oil mixed Mycobacterium butyricum). Buthus martensi Karsch Herbal-acupuncture was injected into ST36(足三里) of rats daily for 21 days. Immunohistological analysis was carried out to assess paw edema, $IL-1{\beta}$, IL-6, IL-8, $TNF-{\alpha}$ and $PGE_2$ expression in synovial membrane and sera Buthus martensi Karsch Herbal-acupuncture injected. Results : Buthus martensi Karsch Herbal-acupuncture group showed a decrease with statistical significance, in paw edema, $IL-1{\beta}$, IL-6, IL-8, $TNF-{\alpha}$ and $PGE_2$ in synovial membrane and sera compared with control group. Conclusion : Buthus martensi Karsch Herbal-acupuncture stimulation inhibited the development of immunity to adjuvant induced arthritis in rats. Thus, Buthus martensi Karsch Herbal-acupuncture may have preventive effects on autoimmune inflammatory joint diseases as arthritis. The effect of Buthus martensi Karsch Herbal-acupuncture on the immune function and the disease activity in patients with arthritis warrants further investigation.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2007.10a
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• pp.105-108
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• 2007

In this paper, we have developed a UPnP-OSGi Bundle for digital convergence based on UPnP middleware. UPnP-OSGi bundle is demanded sustaining realtime monitering system based on UPnP middleware that is possible multimedia ability of a piece with a multiplicity of service and joint user data , service lifecycle , management division service for home network system offered convergence service. This bundle is possible a multiplicity of control and monitering service segmentation so develop a multiplicity of service is easy. and provide zero-configuration system.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.26 no.1
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• pp.7-14
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• 2002

In this study, a stress analysis is made fur an ankle foot orthosis (AFO) in the view point of structural stability. The investigated AFO is a solid and standard type. To analyze the stress distribution on the neck of AFO, the maximum AFO angular translation data is obtained by gait analysis. The material test of polypropylene is made to obtain the mechanical properties of AFO. The maximum dorsiflexion appears at the midstance in the gait analysis. The experimental angular translation at the top of AFO is about 10.3$^{\circ}$ at mid stance. Three models of AFO with different width of neck are made and analyzed with ABAQUS 6.1. The stress levels and distributions of 3 different width(W$_1$, W$_2$=0.85W$_1$, W$_3$=0.60W$_1$) AFO are investigated. As a result, the standard type(W$_1$) appears to the maximum stresses at the medial edge of cutout area surrounding ankle joint. The maximum stresses of the narrower type(W$_2$) are occured on medial edge and center of ankle. The narrowest type(W$_3$) appears to the maximum stresses at center of ankle. The maximum stresses become smaller as ankle width of AFO is narrower.

• Journal of Wetlands Research
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• v.8 no.1
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• pp.97-106
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• 2006

This study discusses two canceled dam projects, Youngwol Dam in South Korea and Two Forks Dam in Colorado of the United States. Both of them illustrate how the new paradigm applies to regional water projects because they became victims of environmental opposition in the new paradigm. While the cases have no apparent close relationships and they occurred in different decades, they offer interesting comparisons. They were basically struggles between water development coalitions and environmental protection coalitions on regional water conflicts. The two proposed projects brought about fierce debates on large dam as they embraced a wide-range of environmental, social, and political issues rather than construction of dams themselves. Huge anti-dam oppositions scrapped them at the cost of nearly ten years for decision-makings and enormous financial resources for feasibility studies respectively. It identifies who the policy actors were, what the policy strategies were, and how the water policies evolved in both countries. The decision-makings on the two projects appeared at first glance to be made under formal institutional frameworks, but in actuality, they relied significantly on decisions of the two important political actors. The Korean society began to learn negotiation and cooperation approaches to solve the water conflict by establishing the Joint Task Force Team on Youngwol project in 1999. The team is recognized as a new conflict resolution method in South Korea because a diverse of stakeholder interests voluntarily participated in the decision-making process and discussed water issues directly. Even though the projects resulted in futile fruits in each country, they illustrate the images of the new paradigm that significantly affected in formulating regional water policies in South Korea and the United States.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.3
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• pp.1390-1395
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• 2014

The objective of this study is to offer a basic data for the establishment of marketing strategies such as fixing price of a new product and creation of the development direction of the product through estimating consumers value by attributes of the newly developed university made multi cream. The Choice Experiment was used for analysis, and conditional logit model was estimated to derive the marginal willingness to pay(MWTP) of each attributes of the multi cream. Brand, container type, functionality, price were included as the attributes. As a result, MWTP were estimated at 21,754 Won/unit for large company product, 11,033 Won/unit for small company product and 16,178 Won/unit for university product, 7,476 Won/unit for enriched moisturizing, 12,107 Won/unit for enriched improvements in wrinkles. Consumers have a preference for university brand over small company brand, therefore, if university and small company cooperate and proceed a joint-venture, it will strengthen the competitive power in the low price brand market. Also, it is essential to develop products with enriched functionalities such as moisturizing and improvements in wrinkles.

• The Journal of Korean Medicine
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• v.28 no.4
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• pp.158-167
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• 2007

Background & Objective : Uncaria rhynchophylla is traditional medicine herb used for enhancing body resistance against various diseases. The aim of this study was to identify if Uncaria rhynchophylla extracts induce osteogenic activity in human osteoblast-like SaOS-2 cells. Methods : The osteogenic activity of Uncaria rhynchophylla was evaluated on cell proliferation assay by WST-8, and osteoblast-specific genes, such as VEGF, type I collagen (Col I), osteocalcin (OCN), and osteopontin (OPN) by RT-PCR analysis and ELISA assay in osteoblasts-like SaOS-2 cells. Bone mineralization was stained with Alizalin red method. Results : Uncaria rhynchophylla had significantly increased cell proliferation at a dose dependent manner in human osteoblast-like SaOS-2 cells. Uncaria rhynchophylla markedly increased alkaline phosphatase (ALP), vascular endothelial growth factor (VEGF) mRNA expression at 7 days and dose dependently increased ALP activity and VEGF secretion in human osteoblast-like SaOS-2 cells. Also, Uncaria rhynchophylla time-dependently increased type I collagen (Col I), osteopontin (OPN), and osteocalcin (OCN) mRNA in SaOS-2 cells. Extracellular accumulation of proteins such as Col I and OCN was maximal increased by Uncaria rhynchophylla at 10 ${\mu}g/ml$. Also, Uncaria rhynchophylla significantly induced mineralization in the culture of SaOS-2 cells. Conclusion : This study showed that Uncaria rhynchophylla had enhanced proliferation, ALP activity, VEGF, bone matrix proteins such as OCN, OPN, and Col I, and mineralization in SaOS-2 cells. These results propose that Uncaria rhynchophylla can play an important role in osteoblastic bone formation, osteogenesis, and may possibly lead to the development of bone-forming drugs.