• BMB Reports
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• 제36권6호
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• pp.572-579
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• 2003

An expressed sequence tag (EST) library was established from the hypopharyngeal glands of Apis cerana. Sixty-six recombinant clones, possessing inserts >500 bp, were randomly selected and unidirectional sequenced. Forty-two of these (63.6%) were identified as homologues of Major Royal Jelly Proteins families 1, 2, 3, and 4 of A. mellifera (AmMRJP) for which MRJP1 was the most abundant family. The open-reading frame of the MRJP1 homologue (AcMRJP1) was 1299 nucleotides that encoded 433 deduced amino acids with three predicted N-linked glycosylation sites. The AcMRJP1 sequence showed 93% and 90% homologies with nucleotide and deduced amino acid sequences of AmMRJP1, respectively. Two complete transcripts of apisimin, and one and two partial transcripts of $\alpha$-glucosidase and glucose oxidase, were also isolated. In addition, the royal jelly proteins of A. cerana were purified and characterized using Q-Sepharose and Sephadex G-200 column chromatography. The native forms of protein peaks A1, A2, B1, and C1 were 115, 55, 50, and 300 kDa, respectively. SDS-PAGE analysis indicated that A1 and C1 were dimeric and oligomeric forms of the 80 kDa and 50 kDa subunits, respectively. The ratio of the total protein quantities of A1 : A2 : B1 : C1 were 2.52 : 4.72 : 1 : 12.21. Further characterization of each protein, using N-terminal and internal peptide sequencing, revealed that the respective proteins were homologues of MRJP3, MRJP2, MRJP1, and MRJP1 of A. mellifera.

• Journal of Community Nutrition
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• 제8권2호
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• pp.107-113
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• 2006

Sensory factors are important determinants of appetite and food choices but little is known about factors affecting taste acuity and preference of Koreans. Any factors causing deficits in sweet taste perception may lead to over consumption of simple sugar, which is related to several chronic diseases. This study was conducted to determine factors affecting sweet taste sensitivity and preference. Subjects were 30 government employees who were serving as school dietitians or in the area of public health while they were studying in the program for the qualification to become nutrition teachers. Sweet taste threshold and the optimally-preferred sweetness of omija jelly were determined by a sensory evaluation and general characteristics, health-related lifestyles, dietary habits and food preferences were determined using a self-administered questionnaire. For the subjects of this study, detection threshold concentration of sucrose solution was $0.184{\pm}0.06%$ and optimally-preferred sucrose concentration of omija jelly was $13.88{\pm}2.28%$ and there was no significant correlation between the sweet taste sensitivity and preference. Subjects who had higher(${\ge}4$ out of 10) physical or psychological stress and who had late getting-up time (after 7am) tended to have lower sweet taste threshold (higher sensitivity) than their counterpart. The sweet taste preference determined by optimally-preferred sucrose concentration of omija jelly tended to be lower in the subjects who eat slowly. Those who answered in the questionnaire to prefer sweet foods did have significantly higher optimally-preferred sucrose concentration of omija jelly. Further research is required to determine whether decreased sensitivity and increased preference for sweetness can increase the actual intake of simple sugar. (J Community Nutrition 8(2): 107 -113, 2006)

• 한국식품영양학회지
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• 제14권5호
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• pp.405-410
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• 2001

영지, 표고, 눈꽃동충하초 그리고 번데기동충하초를 이용한 버섯젤리의 제조와 제품의 색도, 물성 및 기호도를 비교 조사하였다. 버섯 젤리 제조를 위한 추출액의 적정혼합비율을 선발한 결과, 영지버섯 젤리는 버섯추출액 85%, 대추추출액 10%, 황기추출액 5%를 혼합했을 때, 표고버섯 젤리는 버섯추출액 80%, 대추추출액 10%, 감초추출액 5%, 오미자추출액 5%를 혼합했을 때, 눈꽃동충하초와 번데기동충하초 젤리는 버섯추출액 05%, 대추추출액 10%, 감초추출액 5%를 혼합했을 때 가장 우수하였다. 젤화제 종류별로 버섯 추출액에 대한 응고 효과는 모든 버섯에 대해 carrageenan이 가장 효과적이었다 버섯 젤리의 색도는 carrageenan 첨가량에 따라 큰 차이가 없었고, hardness, gumminess, chewiness는 증가하는 경향이었다. 관능은 젤화가 완전히 이루어지면서 hardness가 낮은 carrageenan 0.6% 첨가가 가장 우수하였다.

• 한국수산과학회지
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• 제18권1호
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• pp.44-51
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• 1985

빙장 및 동결저장한 잉어와 붕장어의 어묵 가공원료로서의 적성에 대하여 실험한 것을 요약하면 다음과 같다. 1. K값, VBN, 생균수, pH및 관능검사 결과로 미루어 보아 어묵가공원료로서의 빙장한계는 잉어인 경우 약 16일이었고 붕장어는 약 13 일이었다. 또한 $-30^{\circ}C$, 3개월 동결저장중에는 양시료 모두 그 원료의 품질이 양호한 편이었다. 2. 저온저장에 대한 내성은 잉어가 붕장어보다 다소 강한 편이었다. 3. 제품의 품질특성시험으로서 겔강도, 절곡시험, 보수력, 색조, texture의 측정결과와 관능검사결과로 미루어 보아 빙장시료중 잉어는 빙장 10 일까지 제품품질이 우수하였고 빙장 16 일까지 어묵원료로서 이용 가능하다고 인정되었으며 붕장어인 경우, 빙장 8일까지 제품이 우수하였고 빙장 13일까지 이용가치가 있는 것으로 나타났다. 4. 동결저장한 시료로 만든 어묵제품은 양시료 모두 원료 저장 3개월까지 어묵제품 품질이 양호하였으며, 3개월간 동결저장한 원료의 어묵제품 품질은 잉어인 경우 빙장 $3{\sim}4$일째의 것과, 붕장어는 빙장 $4{\sim}6$ 일째의 것과 그 품질이 각각 서로 유사하였다.

• 한국식품영양과학회지
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• 제44권7호
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• pp.1100-1103
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• 2015

크랜베리 농축액의 첨가량을 0~15%로 달리하여 젤리를 제조한 후 물리화학적 품질, 항산화 특성 및 소비자 기호도를 비교하였다. 크랜베리 농축액의 첨가 비율이 증가함에 따라 젤리의 pH와 경도는 감소하였고, 가용성 고형분 함량은 증가하는 경향을 보였다(P<0.05). 밝기를 나타내는 $L^*$값은 농축액의 첨가량이 증가할수록 유의적으로 감소하였고, 적색도를 나타내는 $a^*$값은 현저하게 증가하였다(P<0.05). 황색도를 나타내는 $b^*$값은 농축액 첨가에 따라 현저하게 감소하였으나, 첨가 농도와 직접적인 연관관계는 발견되지 않았다. 한편 농축액의 첨가량이 증가함에 따라 항산화 활성을 나타내는 DPPH와 ABTS 라디칼 소거능은 유의적 차이를 나타내며 증가하였다(P<0.05). 소비자 기호도 검사 결과 6% 첨가군이 대부분의 평가항목에서 유의적으로 높은 점수를 얻었고, 특히 전체적인 기호도 측면에서도 유의적으로 가장 높게 평가되어 젤리의 관능품질과 건강기능성 효과 등을 고려할 때 최적 첨가 농도로 6%가 가장 적합한 것으로 판단된다.

• 한국식품저장유통학회지
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• 제20권1호
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• pp.62-68
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• 2013

동결건조 로얄제리를 이용하여 세립차를 제조하고자 가공특성을 조사하였다. 동결건조 로얄제리를 이용하여 총당에 대한 포도당 함량($X_1$, 0~100%), 에탄올 농도($X_2$, 75~95%) 및 에탄올 용액 분무량($X_3$, 8~12%)의 비율로 혼합하여 세립형성에 따른 수율, 교반에 의한 분쇄율 및 관능적 특성의 최적조건을 반응표면분석을 통하여 조사하였다. 최대 수율은 총당에 대한 포도당 함량 59.30%, 알콜 농도 88,64% 및 알콜 용액 분무량 11.83%일 때 89.99%로 높게 나타내었다. 교반에 의한 최소 분쇄율은 총당에 대한 포도당 함량 22.35%, 알콜 농도 77.21% 및 알콜 용액 분무량 10.59%에서 0.82%로 낮게 나타내었다. 전반적인 기호도에 대한 최대 관능평점은 총당에 대한 포도당 함량 31.81%, 알콜 농도 93.96% 및 알콜 용액 분무량 10.51%에서 7.45로 나타났다.

• 생약학회지
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• 제45권3호
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• pp.262-267
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• 2014

Royal jelly composes of many components, especially protein. Protein is a major factor which cause allergy. We focused on water soluble royal jelly (WSRJ) that was removed allergy - inducing protein. 10-hyroxy-2-decenoic acid content of WSRJ is 2.42 g/100 g, which is double compared to that of lypophilized RJ. To further access WSRJ as a cosmetic ingredient and potential external treatment for topical use, we investigated its ability to inhibit tyrosinase activity and melanin biosynthesis on melanogenesis in B16F1 melanoma cells. We found that WSRJ increased the cell viability in B16F1 melanoma cell and WSRJ (1~10 mg/ml) inhibited melanin synthesis in with 10 nM ${\alpha}$-melanocyte-stimulating hormone (${\alpha}$-MSH) for 48 h. WSRJ inhibited direct tyrosinase activity, which decreased melanin synthesis in ${\alpha}$-MSH stimulated B16F1 melanoma cells. Thease findings suggest that WSRJ induces the down regulation of melanogenesis by inhibiting tyrosinase activation.

• 한국식품과학회지
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• 제23권3호
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• pp.336-340
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• 1991

시판되고 있는 7종의 젤리를 가지고 rheometer를 사용하여 저작실험을 한 결과 관능적 물성인 hardness 및 shortness와 가장 상관제수가 높은 기계적 물성은 maximum force로서 2900-3136($10^3\;dyne$) 범위에서 적당하다고 나타났으며 또한 stickiness는 adhesiveness force와 관능적인 물성 springiness는 기계적 물성값 springiness로 예측할 수 있었다. 그러나 종합평가와의 회귀식에서 얻은 적당하다고 계산된 값과는 약간의 차이를 나타내었으며 특히 종합평가와 결정계수가 가장 높은 기계적 물성은 cohesiveness이었다. 종합평가와 각 기계값과의 관계에서 종합평가에 가장 영향을 미치는 기계값은 cohesiveness, adhesiveness force로 이들의 결정계수는 각각 0.93, 0.89이었으며 종합평가를 종속변수로 하고 cohesiveness 및 adhesiveness를 독립변수로 하여 multiple linear regression 한 결과 종합평가=2.70+6.25(cohesiveness) -0.095(adhesiveness)의 상관식을 얻었으며 이 때의 중상관계수 0.94이었다.

• Journal of Dental Anesthesia and Pain Medicine
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• 제20권6호
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• pp.397-402
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• 2020

Background: Inferior alveolar nerve block (IANB) is the most common, painful, and anxiety-provoking procedure involving needle insertion for anesthetic solution deposition. DentalVibeⓇ (DV) delivers vibration at a sustained frequency as a counter-stimulation to the site of injection, thereby alleviating pain. The aim of this study was to evaluate and compare the effectiveness of DV and lignocaine hydrochloride 2% gel (Lox 2% jelly) in pain reduction during IANB in children. Methods: A split-mouth randomized clinical trial was designed with a sample of 60 children (age, 6 to 12 years) requiring bilateral IANB for various dental procedures; DV was used while administering IANB and Lox 2% jelly was used as the topical anesthetic before administering IANB at subsequent appointments. During both appointments, pain perception was measured using the sound, eye, motor (SEM) scale and Wong-Baker faces pain rating scale (WBFPRS); oxygen saturation (SpO2) and pulse rate were measured using a pulse oximeter before, during, and after the IANB procedure. The obtained values were tabulated and subjected to statistical analysis. Wilcoxon test was used for intergroup comparison, and Friedman test, for intragroup comparison of measured variables at different treatment phases. Results: The medians and interquartile ranges of the WBFPRS scores recorded during the IANB procedure for DV and Lox 2% jelly were 2 (2-4) and 2 (0-2), respectively (P < 0.05). The SEM scale scores, mean SpO2, and pulse rate did not show any significant differences during the IANB procedure between both treatments. Conclusion: Both DV and Lox 2% jelly were found to be effective in pain reduction during IANB in children.

• Preventive Nutrition and Food Science
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• 제14권2호
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• pp.148-155
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• 2009

The purpose of this study was to find new applications for Rubus coreanus and to produce new products. Jelly prepared with Rubus coreanus was studied and analyzed with regard to its composition, rheology and sensory evaluation. The technique of response surface methodology was used to analyze the results, which showed 16 experimental points including 2 replicates for Rubus coreanus juice, gelatin and sucrose. Rheological characteristics such as lightness, redness, yellowness and hardness, springness, chewiness and gumminess were shown to be significant, and these were mostly influenced by the content of Rubus coreanus juice. Significant sensory characteristics such as color, flavor, transparency, taste and the overall quality were also found to be greatly influenced by Rubus coreanus juice and gelatin in general. Based on the overlapping categories of factors that satisfied all sensory categories, the maximally optimized point was taken as the middle point. This value was determined to be 43.28 g of Rubus coreanus juice and 9.19 g of gelatin for every 140.00 g of sucrose.